Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

S-344 : The Role of Skeletal Muscle Mass in Development of Metabolic Syndrome

( Byung Sam Park ),( Jun Sung Moon ),( Ji Sung Yoon ),( Kyu Chang Won ),( Hyoung Woo Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Introduction: It has been well known that abdominal adiposity is related with insulin resistance and greater risk of metabolic disorder. Skeletal muscle plays central role in insulin mediated glucose disposal of whole body, but we only know a little about the association between skeletal muscle mass and MetS (MetS). The aim of this study is to clarify the clinical role of skeletal muscle mass in developing MetS through using skeletal muscle parameters by body impedance analysis (BIA). Methods: 1,042 healthy adults aged from 20 to 75 years who visited Yeungnam university health promotion center from Jun. 2008 to Jun. 2010 were enrolled. 204 subjects who had prior MetS or chronic disease that can affect to skeletal muscle mass were excluded. After 24 months from baseline, the metabolic parameters were assessed and the development of MetS was diagnosed using modified NCEP-ATP III criteria. From the BIA (Inbody 720), we obtained skeletal muscle mass (SMM, Kg), body fat mass (BFM, Kg), and visceral fat area (VFA, cm2). Then, we had defined as follows; percent of skeletal muscle mass (SMM%, %): SMM (kg)/weight (Kg), skeletal muscle index (SMI, Kg/m2): SMM (Kg)/height (m)2, skeletal muscle to body fat ratio (MFR): SMM (kg)/BFM (Kg) and skeletal muscle to visceral fat ratio (SVR, Kg/cm2): SMM (Kg)/VFA (cm2) Results: Mean follow up periods were 28.7±5.4 months. Among total 838 subjects (46.9±9.9 years, M:F=477:361), 88 (10.5%) were newly diagnosed MetS. 5th quintile of the SMM%, MFR and SVR was associated with decreased risk of development of MetS after adjusting for confounding factors. Conclusion: Decreased skeletal muscle mass may play critical role in development of the MetS and not absolute amount of skeletal muscle mass but relative ratio to body composition may be more important.

창조도시 전략으로서의 문화예술교육 : 창조적 학습도시 발전전략에 대한 새로운 논의

이병준,박응희,박지연 한국문화교육학회 2010 문화예술교육연구 Vol.5 No.2

최근 창조도시논의가 활발해지면서 교육과 학습의 창조도시와의 연관성에 주목할 필요가 높아졌다. 2000년 이후 문화예술교육진흥을 위한 법과 제도가 정비되면서 문화예술교육이 학교와 사회속에서 확산되고 있다. 그러나 단순히 문화예술교육을 평생교육의 연장선속에서에 대한 논의하는 것도 의미가 있지만 문화와 예술을 기반으로 하는 도시발전전략인 창조도시와의 연관성속에서 바라보는 것도 흥미롭다고 볼 수 있다. 현재 창조도시논의는 크게 창조적 환경에 대한 논의, 창조인력에 대한 논의, 창조산업에 대한 논의, 그리고 문화발전에 대한 논의 네 가지로 전개되고 있다. 이러한 창조도시에 대한 논의중 문화와 예술을 경제에 활용하는 의미에서의 창조산업에 대한 논의가 가장 활발하나 이러한 논의는 매우 근시안적 사고라고 판단된다. 창조도시는 개별 도시가 가지는 자발적 시스템속에 창조성과 지역성, 네트워크와 미학화, 그리고 학습과 지속가능성이라는 핵심가치를 추구하고 있으며 또 그래야 한다. 이러한 측면에서 창조도시의 핵심가치는 바로 문화예술교육과 매우 밀접한 관련을 가지고 있다. 현재의 문화예술교육은 단순한 프로젝트성 보다는 창조도시를 만들어가기 위한 중요한 전략으로 인식될 필요가 있다. 따라서 문화예술교육은 장기적인 비전속에 지속가능성을 담보해야 하며 예술체험을 넘어서 문화역량을 강화하고 학습망을 구축하며 문화리더를 양성할 수 있는 방향으로 나아가야 할 것이다. As the discussion on creative city has become active recently, the necessary to pay attention to the correlation between education/learning and creative city was increased. While law and system for promoting Culture & Arts Education have been reorganized since 2000, Culture & Arts Education spreads into schools and society. However, it's meaningful to discuss Culture & Arts Education simply in an extension of lifelong education but it's interesting to examine it in the correlation with creative city which is a strategy to develop the city based on culture and arts. At present, the discussion on creative city is unfolded by dividing largely into 4 discussions; discussion on creative environment, discussion on creative man power, discussion on creative industry and discussion on cultural development. Among the discussions on creative city, the discussion on creative industry which means to apply culture and arts to economy is the most active but it is judged to be a very short-sighted thinking. The creative city is seeking core values such as creativity / regional characteristic, network / aestheticization and studying / sustainability in the voluntary system of each city as well as must to seek them. Those core values of creative city are related with Culture & Arts Education much closely in that aspect. Current Culture & Arts Education should be recognized by an important strategy for making creative city, rather than by simple project. Therefore, Culture & Arts Education must to guarantee sustainability in a long-term vision, strengthen cultural capability beyond the art experience, establish learning network and advance to the direction cultivating cultural leaders.

진료비 상담의 효율적인 업무개선을 통한 고객만족도 향상

서지연,채은경,고영준외 39인 한국의료QA학회 2006 한국의료질향상학회지 Vol.13 No.2

문제: 최근 몇 년간, 환자 및 보호자들이 의료와 관련된 전문기관을 대상으로, 민원발생이 급, 상승되고 있음이 통계상 확인되고 있다. 목적: 최근 몇 년간, 환자 및 보호자들이 의료와 관련된 전문기관을 대상으로, 민원발생이 급, 상승되고 있음이 통계상 확인되고 있다. 의료기관: 서울에 소재한 2,200병상의 전문종합요양기관 소속 보험 심사 팀 질 향상 활동: 상담 Data base 구축을 위한 전산개발 및 핵심 문제 부분에 대한 직무프로세스 변경, 보완 및 수정 활동결과: 진료비 사례 환자 상담건수는 2003년 기준 75.4%의 증가율을 보였으며, 본원의 민원검수는 전년대비 9%의 감소효과가 확인되었다.

활동이론의 관점에서 바라본 박물관, 커뮤니케이션, 그리고 학습

박지연,이수민,이병준 한국문화교육학회 2011 문화예술교육연구 Vol.6 No.4

Museum is a medium which communicates with previous generation's experiential memories and present generation's cultural memories. Therefore, the communication is a core task in museum. Museum needs mediums like exhibition and education and we have to discuss how to structuralize these things. The museum communication could be explained through the Cultural-Historical Activity Theory(CHAT). The CHAT has been developed by several theorists like Vygotsky. Especially, the third generation's CHAT has been developing by Engeström. It is a worthy theoretical structure that can show museum as a messenger of communication in society and its communication problems. This study is based on the CHAT and analyzes museum in a social activity system and activity system in a museum from micro/macro-scopic view. Also, it produces practical models that connect museum with community art field and museum with schools. 박물관은 앞선 세대의 체험적 기억을 현 세대에게 문화적 기억의 형태로 커뮤니케이션하는 매개체이다. 따라서 박물관에서 소통(communication)이라는 개념은 핵심적인 과제일수 밖에 없다. 박물관은 그 자체로 전시와 교육과 같은 매체(medium)를 필요로 하며 이를 어떻게 구조화할 것인가의 문제가 박물관커뮤니케이션의 측면에서 논의될 필요가 있다. 이러한 거시적, 미시적 차원의 박물관커뮤니케이션의 과제는 비고츠키와 여러 이론가들의 연구성과를 정리하고 발전시킨 활동이론에 의해 잘 설명될 수 있을 것으로 보인다. 특히 Y. Engeström에 의해 발전된 제3세대 활동이론은 사회속에서의 커뮤니케이션의 전달자로서의 박물관과 박물관내에서의 커뮤니케이션의 문제를 잘 드러낼 수 있는 이론체계라고 생각된다. 본 연구는 활동이론에 근거하여 거시적·미시적 관점에서 사회활동시스템 속에서의 박물관과 박물관 내에서의 활동시스템을 분석하고 이를 통해 박물관의 커뮤니케이션을 박물관 내부 활동시스템 간의 네트워크 실천, 박물관과 지역의 예술장(藝術場)이 연계된 네트워크 실천과 박물관과 학교가 연계된 네트워크 실천 모형을 구조화하여 제시하려 한다.

의료사고에서 의료인의 역할분담에 따른 형사법적 책임에 관한 연구

전지연 한림대학교 법학연구소 2000 한림법학 FORUM Vol.9 No.-

컴퓨터犯罪에 대한 刑法的 對應

全智淵 충북대학교 법학연구소 1998 Juris Forum Vol.1 No.1

위증죄에서 진술의 허위성

全智淵 한림대학교 법학연구소 1998 한림법학 FORUM Vol.7 No.-

제어용 전원으로 사용 가능한 공진형 컨버터 시스템

지준근,임영하 순천향대학교 부설 산업기술연구소 2003 순천향 산업기술연구소논문집 Vol.9 No.1

In this paper new control strategy of series resonant converter system for control power supply is suggested. Frequency controlled series resonant converter system is robust to load variations because it is POSR(parallel output series resonant) type. And it provides stable output voltage by changing switching frequency to input voltage variations. Firstly, operation analysis about suggested series resonant converter system was carried. Then simulations using ACSL(Advanced Continuous Simulation Language) and experiments to actual system were carried to prove characteristics of suggested system.