The Butanol Fraction of Bitter Melon (Momordica charantia) Scavenges Free Radicals and Attenuates Oxidative Stress

Kim, Hyun Young,Sin, Seung Mi,Lee, Sanghyun,Cho, Kye Man,Cho, Eun Ju The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.1

To investigate radical scavenging effects and protective activities of bitter melon (Momordica charantia) against oxidative stress, in vitro and a cellular system using LLC-$PK_1$ renal epithelial cells were used in this study. The butanol (BuOH) fraction of bitter melon scavenged 63.4% and 87.1% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals at concentrations of 250 and $500{\mu}g/mL$, respectively. In addition, the BuOH fraction of bitter melon effectively scavenged hydroxyl radicals (${\cdot}OH$). At all concentrations tested, the scavenging activity of the BuOH fraction was more potent than that of the positive control, ascorbic acid. Furthermore, under the LLC-$PK_1$ cellular model, the cells showed a decline in viability and an increase in lipid peroxidation through oxidative stress induced by pyrogallol, a generator of superoxide anion ($O_2{^-}$). However, the BuOH fraction of bitter melon significantly and dose-dependently inhibited cytotoxicity. In addition, 3-morpholinosydnonimine (SIN-1), a generator of peroxynitrite ($ONOO^-$) formed by simultaneous releases of nitric oxide and $O_2{^-}$, caused cytotoxicity in the LLC-$PK_1$ cells while the BuOH fraction of bitter melon ameliorated oxidative damage induced by $ONOO^-$. These results indicate that BuOH fraction of bitter melon has protective activities against oxidative damage induced by free radicals.

담배연기성분 중 니코틴함량 측정결과에 대한 불확도 산정

김영신,정재훈,임흥빈,이학수,석영선,신주식 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

담배성분에 대하여 정량분석을 하여 구하는 값은 측정하고자 하는 대상의 참값에 대한 추정값이며, 이는 이상적인 참값으로부터 여러 가지 원인에 의해 벗어나게 된다. 실험 분석 결과는 실험대상에 대한 여러 가지 판단 근거를 제공하는 것이며, 이에 대한 신뢰성의 척도로 불확도가 사용되고 있다. 따라서, 본 연구는 담배연기성분 중 니코틴함량 측정결과에 대하여 신뢰성을 부가시키고, 더욱이 실험과정에서 중요한 불확도의 원인을 찾아내어 해당부분의 불확도를 줄임으로써 보다 정확한 분석결과를 얻고자 이루어졌으며 이 방법으로 추정된 CM4의 측정값과 불확도 표현은 다음과 같다. Nicotine : 1.28 ± 0.01 mg/cig. (신뢰 구간 95.45%, k=2) Measurements by quantitative analysis of tobacco components were the presumed value of a true value. But this measurement was out of ideal true value by various reasons. The aim of this study was to invest with confidence to measurement of nicotine content in tobacco smoke components and to reduce uncertainty by searching uncertainty reasons. Uncertainty of nicotine contents of CORESTA monitor 4 by this method was as follows.

급성 운동 신경 축삭성 신경병증과 동반된 기관지내 이물 1예

조영신,전웅,이호성,최재성,나주옥,서기현,김용훈 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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SiN_<i>x</i>Double Layer Antireflection Coating by Plasma-Enhanced Chemical Vapor Deposition for Single Crystalline Silicon Solar Cells

Gong, Daeyeong,Lee, Youn-Jung,Ju, Minkyu,Ko, Jisoo,Yang, Doohwan,Lee, Yongwoo,Choi, Gyuho,Kim, Sin,Yoo, Jinsu,Choi, Byoungdeog,Yi, Junsin IOP Publishing 2011 Japanese journal of applied physics Vol.50 No.8

우리나라 야생조류의 체내 납(Pb)과 카드뮴(Cd) 축척

김정수,신주렬,이화수,구태회 경희대학교 2006 環境硏究 論文集 Vol.15 No.-

This study presents concentrations of lead and cadmium in liver and kidney in Korean wild birds during 2000-2002. Lead concentrations in livers were Kharadriiformes < Anserifoimes < Kiconiiformes < Falconiformes < Passeriformes < Columbiformes. Their lead concentrations in livers and kidneys was within background level and exposed level. Cadmium concentrations in kidneys were Kharadriiformes < Ciconiiformes < Anseriformes < Falconiformes < Passeriformes < Columbifoimes. Kidney cadmium concentrations in Korean wild birds were within the range of background level, but one Cattle Egret Bibulous ibis and 7 Ancient Murrelet Synthliboramphus antiques was toxic level.

전문비서란 무엇인가? : Its Concept and Function

김주연,용주현,신동희,유민,최정윤 이화여자대학교 법정대학 비서학과 1995 비서학연구 Vol.- No.15

페놀베타인 유도체합성(XI)

우성주,박예진,황순호,홍유화,김남재,김인종,김신규 慶熙大學校 1996 論文集 Vol.25 No.-

Tetrahydroberberine-13-one was treated with ethylchloroformate to give the C_8-N bond cleaved product 1. On the treatment with Lawesson's reagent (LAS), chloroketone 2 derived from the hydrolysis of 1 afforded the thioketone 3. On the other hand, spirotype-chloroketone 4 was transformed to diaziridine 8 by a reduction, chlorination and aziridine introduction. Also, spirotype-chlorothioketone 6 was obtained from chloroketone 4.

β-naphthol 유도체 합성 및 항종양활성 검토

이주영,류수진,박예진,황순호,이마세,김인종,김동현,김신규 慶熙大學校 1996 論文集 Vol.25 No.-

Sythesis of β-naphthol derivatives and their anti-tumor activity were investigated. Binaphthol 1 obtained from β-naphthol by oxidative C-C bond formation(phenoloxydation) was converted into its derivatives. Treatment of 1 with POCl_3 followed by aziridine introduction gave phosphoryl aziridine 3. Also, diaziridine 5 was obtained from 1 by chlorination and successive aziridine introdution. Typical chemical transformation of 1 to obtain ester-type afforded compound 7 and 8. Compound 5 was the most effective derivative of the tested compounds on their anti-tumor activity.

노인의 수면양상과 관련요인에 관한 연구

오진주,김신미,송미순 서울대학교 간호대학 간호과학연구소 1997 간호학 논문집 Vol.11 No.1

Sleep is an essential component in health and the quality of life of individual. Normally, sleep pattern changes with advancing age. These changes often result in sleep impairment and become a major stressor in elderly. Even though the elderly is the fastest growing population, sleep impariment among elderly has not been well documented, few studies have been conducted related to the sleep of the elderly in Korea. The purpose of this study is to explore sleep pattern, related factors, and sleep satisfaction among healthy community-dwelling elderly, in order to present basic informations regarding Korean elder's sleep. 170 subjects over 60-yer-old were participated in the study. The survey questionnaire was developed by translation and modification of two well-known sleep questionnaires which originally developed by Monroe(1967) and Ellis, et al.(1982). Pilot study was done liefore final contents of a questionnaire was organized. SPSS/PC+ was utilized for data analysis. Results as follows: 1. Sleep pattern changes were reported by 75% of the subjects: decline in sleep time: frequent awakening: difficulty in falling asleep; lack of deep sleep; falling asleep early and getting up early; sleepy; decline in early morning sleep; irregular sleep habit; early retiring in bed. 2. Most of the subjects reported to fall asleep between 9-11pm, and get up between 4-6am. Forty point one percent of the subjects stated that they have problems in falling asleep. More than 70% reported their sleep to be satisfied. Sixty one point eight responded that they hardly or never take a nap. 3. Regarding sleep-disturbing factors, physical factor(30.1%), emotional factor(44.2%), environmental factor(41.0%) were reported. 4. Nineteen point two of the subjects practiced some types of strategies for better sleep. But a few(12.8%) used hypnotics, mainly in occasional base. 5. Variables showed statistically significant differences in sleep satisfaction were; getting up right away upon waking; frequency of awakening during night time sleep; difficulty in falling asleep; feeling rested upon waking int eh morning ; difficulty in going back to sleep upon awakening during night time sleep. From the results of this study, it can be concluded that the elderly in the community do have some sleep impairment but do not practice adequate strategies for better sleep. Further research are needed to develop better sleep measurement tool, to compare sleep pattern with other age groups and to develop nursing intervention.

발아율 향상을 위한 종자처리 중 담배 종자의 지방산 변화

신주식,김영신 충북대학교 연초연구소 2006 煙草硏究 Vol.20 No.-

본 연구에서는 발아력 향상을 위해 담배품종 Nicotiana tabacum cv. KF109와 cv. KB108 종자에 종자전처리방법 중 하나인 solid matrix priming (SMP)처리를 하여 전처리 효과를 알아보고 전처리과정 중 담배종자 발아시 에너지원으로 사용되는 지방산의 함량 변화를 조사한 결과 아래와 같다. 1. 발아시험결과 전처리에 의한 처리간 발아율 차이는 보이지 않았으나, 발아세, T50은 KF109의 경우 9일처리, KB108의 경우 7일처리에서 가장 높았다. 2. 종자내 지방산을 분석한 결과 처리기간동안 함량의 변화가 큰 지방산의 종류는 palmitic acid, stearic acid, oleic acid+elaidic acid, linoleic acid+linolelaidic acid, α-linole nic acid였다. 3. 지방산 종류에 따라 다소 차이가 있으나 각 지방산의 함량은 KF109의 경우 8일 또는 9일에서, KB108의 경우 6일 또는 7일까지 증가하였다가 이후 감소하였다. 4. 특히 지방산 중 oleic acid+elaidic acid와 linoleic acid+linolelaidic acid 함량의 변화가 가장 컸으며 다른 지방산에 비하여 함량도 높았는데 KF109와 KB108 품종의 경우 8일과 6일처리까지 각각 증가하여 최고에 이른 후 급격히 감소하는 결과를 보여 종자전처리 진행과정을 확인하는 데에 이용 가능할 것으로 판단된다. We studied the effect of SMP(solid matrix priming) treatment, a pretreatment for germination enhancement, to tobacco seeds by measuring of germination rate and fatty acid, energy source for tobacco germination. The results were as follows. In result of germination test, germinative ability was the highest in KF109 at nine day treatment and in KB108 at seven day treatment. The composition of fatty acid in tobacco seed confirmed by gas chromatography were palmitic acid, stearic acid, oleic acid+elaidic acid, linoleic acid+linolelaidic acid, and a-linolenic acid. Palmitic acid, stearic acid, oleic acid and elaidic acid, and linoleic acid and linolelaidic acid were highest in KF109 at eight or nine day treatment and in KB108 at six or seven day treatment. Especially, content of oleic acid + elaidic acid, and linoleic acid+linolelaidic acid were changed largely by treatment, so these might be used for index to examine treatment effect.