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An ultra-effective method of generating extramultipotent cells from human fibroblasts by ultrasound
Lee, Yong Seung,Heo, Hyejung,Lee, Jonghwan,Moon, Sung Ung,Jung, Woon Yong,Park, Yong Keun,Park, Min Geun,Oh, Seung-Hun,Kim, Soonhag IPC Science and Technology Press 2017 Biomaterials Vol.143 No.-
<P><B>Abstract</B></P> <P>Multipotent cells have similar basic features of all stem cells but limitation in ability of self-renewal and differentiation compared with pluripotent cells. Here, we have developed an ultra effective, gene- and chemical-free method of generating extra multipotent (xpotent) cells which have differentiation potential more than limited cell types, by the mechanism of ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr). Ultrasound stimulus generated a massive number of Entr-mediated xpotent (x/Entr) spheroids from human dermal fibroblasts (HDFs) 6 days after treatment. The emergence of x/Entr was first initiated by the introduction of human embryonic stem cell (ESC) environments into the HDFs to start fast cellular reprogramming including activation of stress-related kinase signaling pathways, subsequent chromatin remodeling, and expression of pluripotent-related genes via transient membrane damage caused by ultrasound-induced cavitation. And then, pluripotent markers were transported into their adjacent HDFs via direct cell-to-cell connections in order to generate xpotent clusters. The features of x/Entr cells were intermediate between pluripotency and multipotency in terms of pluripotency with three germ layer markers, multi-lineage differentiation potential, and no teratoma formation. This physical stimulus-mediated reprogramming strategy was cost-effective, simple, quick, produced significant yields, and was safe, and can therefore provide a new paradigm for clinical application.</P>
A self-assembling magnetic resonance beacon for the detection of microRNA-1
Lee, Jonghwan,Kang, Hyo Jin,Lee, Yong Seung,Heo, Hyejung,Gu, Ha-Na,Cho, Sujeong,Kim, Soonhag The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.33
<P>A self-assembling magnetic resonance beacon was used to visualize the microRNA-1 expression-dependent change in magnetic resonance signal intensity.</P> <P>Graphic Abstract</P><P>A self-assembling magnetic resonance beacon was used to visualize the microRNA-1 expression-dependent change in magnetic resonance signal intensity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc10231b'> </P>