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Na, Jong-Kuk,Kim, Jae-Kwang,Kim, Dool-Yi,Assmann, Sarah M. Oxford University Press 2015 Journal of experimental botany Vol.66 No.13
<P>The <I>Arabidopsis thaliana</I> genome encodes three RNA-binding proteins (RBPs), UBP1-associated protein 2a (UBA2a), UBA2b, and UBA2c, that contain two RNA-recognition motif (RRM) domains. They play important roles in wounding response and leaf senescence, and are homologs of <I>Vicia faba</I> abscisic-acid-activated protein kinase-interacting protein 1 (VfAKIP1). The potato (<I>Solanum tuberosum</I>) genome encodes at least seven AKIP1-like RBPs. Here, two potato RBPs have been characterized, StUBA2a/b and StUBA2c, that are homologous to VfAKIP1 and <I>Arabidopsis</I> UBA2s. Transient expression of <I>StUBA2</I>s induced a hypersensitive-like cell death phenotype in tobacco leaves, and an RRM-domain deletion assay of StUBA2s revealed that the first RRM domain is crucial for the phenotype. Unlike overexpression of <I>Arabidopsis UBA2</I>s, constitutive expression of <I>StUBA2a/b</I> in <I>Arabidopsis</I> did not cause growth arrest and lethality at the young seedling stage, but induced early leaf senescence. This phenotype was associated with increased expression of defence- and senescence-associated genes, including pathogen-related genes (<I>PR</I>) and a senescence-associated gene (<I>SAG13</I>), and it was aggravated upon flowering and ultimately resulted in a shortened life cycle. Leaf senescence of <I>StUBA2a/b Arabidopsis</I> plants was enhanced under darkness and was accompanied by H<SUB>2</SUB>O<SUB>2</SUB> accumulation and altered expression of autophagy-associated genes, which likely cause cellular damage and are proximate causes of the early leaf senescence. Expression of salicylic acid signalling and biosynthetic genes was also upregulated in <I>StUBA2a/b</I> plants. Consistent with the localization of UBA2s-GFPs and VfAKIP1-GFP, soluble-modified GFP-StUBA2s localized in the nucleus within nuclear speckles. StUBA2s potentially can be considered for transgenic approaches to induce potato shoot senescence, which is desirable at harvest.</P>
Na, Jong-Kuk,Seo, Mi-Hye,Moon, Seok-Jun,Yoon, In-Sun,Lee, Yeon-Hee,Kim, Jae Kwang,Lee, Kyun Oh,Kim, Dool-Yi 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
Polycomb group (PcG) proteins form Polycomb Repressive Complex 2 (PRC2), which regulates seed development by the epigenetic control of gene expression. Interaction assay among Arabidopsis Fertilization-independent-seed2 (FIS) class PcG proteins showed that Fertilization- independent endosperm (FIE) interacts with Medea (MEA), a SET-domain polycomb protein, of which N-terminal region is crucial for the interaction. In this study, rice SET-domain PcG protein OsEZ1, also known as OsiEZ1 in indica rice, was analyzed to identify an interacting domain of OsEZ1 required for OsEZ1-OsFIE2 protein interaction. A series of OsEZ1 deletions were generated and used to determine an interacting domain of OsEZ1 with OsFIE2 using the yeast two-hybrid system. Among OsEZ1 deletions, only OsEZ1D2 and OsEZ1D3 interacted with OsFIE2, indicating that the 155K-169R or N-proximal region of OsEZ1 is crucial for OsFIE2-OsEZ1 interaction. To examine the physiological roles of OsEZ1, 35S:OsEZ1 Arabidopsis lines were generated. OsEZ1 overexpressors exhibited altered seedling growth and seed size, implying that OsEZ1 may play important roles in seedling and seed development.