양파농축액에 대한 일부 항갈색화제의 효과

손종연(Jong-Youn Son),손홍수(Heung-Soo Son),조원대(Won-Dai Cho) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.3

양파농축액에 대한 ascorbic acid, cystine citric acid 등의 갈색화 억제 효과 및 이들간의 조합에 의한 갈색화 억제에 대한 상승작용의 여부를 조사하고자 하였다. 양파농축액에 대한 갈색화 억제 효과는 cysteine 첨가의 경우 가장 높게 나타났으며 citric acid 첨가의 경우 비교적 낮았다. 또한 ascorbic acid 첨가시 오히려 갈색화를 촉진시켰다. Cysteine은 모든 첨가 농도(0.1~0.5%)에서 갈색화 억제 효과를 보였으나 0.3% 이상의 농도에서의 억제 효과는 큰 차이를 보이지 않았다. Cysteine에 대한 citric acid의 상승작용은 ascorbic acid 보다 큰 것으로 나타났다. 갈색화 반응에 대한 활성화 에너지는 cysteine과 citric acid를 첨가한 양파농축액이 62J/㏖, 대조 양파농축액이 73J/㏖로 대조구에 비해 낮았으며, 온도계수 또한 cysteine과 citric acid를 첨가한 양파농축액이 대조구 보다 0.3 정도 낮게 나타났다. Using cystine, ascorbic acid and citric acid, retarding and synergistic effect on the browning reaction of onion juice concentrate was evaluated. Cysteine retarded the browning reaction most effectively followed by citric acid; on the contrary, ascorbic acid accelerated rather than retarded the reaction at the concentration of 0.1%. The retarding effect of cysteine increased abruptly up to concentration of 0.3% and remained unchanged. Citric acid acted more effectively than ascorbic acid in terms of synergism. The browning reaction of onion juice concentrate exhibited activation energies of 62J/㏖ with cysteine and citric acid as compared to 73J/㏖ for control. Q_(10) was determined to be 2.52 and 2.38 for control, 2.2 and 2.09 for treatment in the temperature range of 30~40℃ and 40~50℃, respectively.

Targeted doxorubicin nanotherapy strongly suppressing growth of multidrug resistant tumor in mice

Nguyen, Dai Hai,Lee, Jung Seok,Bae, Jin Woo,Choi, Jong Hoon,Lee, Yunki,Son, Joo Young,Park, Ki Dong Elsevier 2015 International journal of pharmaceutics Vol.495 No.1

<P><B>Abstract</B></P> <P>The rational design of nanomedicine to treat multidrug resistant (MDR) tumors <I>in vivo</I> is described in the study. We prepared multifunctionalized Pluronic micelles that are already well-established to be responsive to low pH and redox in order to systemically deliver doxorubicin (DOX) to MDR tumors. Folic acids (FAs) were introduced on the micelle surface as tumor-targeting molecules. In vitro, the DOX-loaded micelles exerted high cytotoxicity in the DOX-resistant cells by bypassing MDR efflux. Cellular uptake studies clearly demonstrated that FA-conjugated DOX micelles (FA/DOX micelles) were efficiently internalized and accumulated in the MDR cells. In vivo studies indicated significant efficacy of FA/DOX micelles for MDR tumors in mice, and that the volume of tumors was 3 times smaller in this group than that of tumors in the free DOX group, and 8 times smaller than the tumors in the saline group. To the best of our knowledge, this methodology has been recognized to have significantly high efficacy, compared to previously reported DOX nanoparticle formulations. This superior anti-tumor efficacy of FA/DOX micelles in MDR tumor-bearing mice can be attributed to FA-targeted and -mediated endocytosis, inhibition of MDR effect, and subsequent DOX release triggered by dual stimuli (low pH and redox) inside the tumor. Given the promise of the multifunctional micelle mediated delivery on inhibition of MDR tumor growth, FA/DOX micelle platform is a much sought after goal for cancer chemotherapy, especially for cancers resistant to anticancer drugs.</P> <P><B>Graphical abstract</B></P> <P>Folic acid (FA) and doxorubicin (DOX)-conjugated micelles (FA/DOX micelles) that are responsive to pH and redox show superior anti-tumor efficacy in multidrug resistant (MDR) tumor-bearing mice because of FA-targeted and -mediated endocytosis, inhibition of MDR efflux, and subsequent DOX release triggered by dual stimuli (low pH and redox) inside the tumor. The volume of tumors was 8 times smaller in the micelle group than the tumors in the saline group.</P> <P>[DISPLAY OMISSION]</P>

양파껍질 추출물의 항산화 및 상승효과

조원대,손흥수,손종연 한국조리과학회 1998 한국식품조리과학회지 Vol.14 No.1

The antioxidant and synergistic effect of methanol extracts of onion skin were investigated by measuring peroxide value. The inhibitory effects of the extracts against metal catalyzed oxidation were also studied. The antioxidant activity of methanol extract (0.02%) of onion skin was stronger than that of mixed tocopherol or ascorbic acid (0.02%), but weaker than that of BHT (0.02%). However, the methanol extract at the concentration of 0.04% exhibited strong antioxidant effect, comparable to that of BHT (0.02%). The methanol extract showed very strong synergistic effect with the mixed tocopherol. The methanol extracts at the concentration of 0.03% and 0.04% acted as metal deactivator in the presence of FeCl₃. As a metal deactivator, methanol extract of onion skin appeared to be more effective than citric acid.

Changes of HPLC Pattern according to the Induction of Hairy Roots and the Treatment of Methyl Jasmonate in Cirsium setidens and C. pendulum

Tae Gyu Yi(이태규),Jong Dai Son(손종대),Yeri Park(박예리),Byung Hun Um(엄병헌),Nam Il Park(박남일) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

고려엉겅퀴와 큰엉겅퀴의 모상근 유도 및 methyl jasmonate 처리에 따른 HPLC 패턴 변화

Tae Gyu Yi,Jong Dai Son,Yeri Park,Byung Hun Um,Nam Il Park 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05

Background : Cirsium plants have been used for perennial edible plants and grow wild in the mountainous regions of Korea, including Cirsium setidens and C. pendulum. In particular, C. setidens is commonly called 'gondre', and it has been used medicinally for diseases such as hematuria, hepatitis and hypertension. Hairy roots cultivation can be used as a method for increasing production through mass culture of medicinal plants, and elicitors such as methyl jasmonate (MeJa) can be treated to increase the content of certain useful ingredients. In this study, the hairy root was derived from the leaf tissues of C. setidens and C. pendulum, and the HPLC pattern was compared by MeJa treatment. Methods and Results : Agrobacterium rhizogenes R1000 was used to induce hairy roots in 1/2× MS medium. In addition, the hairy roots was treated with MeJa for different time (0, 1, 2, 5, 10, 24, 48, 72 h) and with various concentrations (0, 10, 50, 100, 200, 300 μM). The HPLC pattern changes were analyzed by on-line HPLC-ABTS. Four new peaks were observed in both Cirsium setidens and C. pendulum hairy roots, all of which showed antioxidant activity. In the case of C. pendulum, chlorogenic acid content was about 4 times higher than that of leaves. These peaks, including chlorogenic acid, were all affected by MeJa treatment. Conclusion : Four peaks were detected in the hairy roots of C. setidens and C. pendulum not in the leaves, and they were confirmed to be affected by the treatment of MeJa. It is necessary to clarify the structure through the subsequent compound separation.

Effect of Cultivars and Cooking Methods on the Trypsin Inhibitor Activities of Potatoes

Mi Yeon Kim,Chan Wok Son,Hyun Jung Shim,Jeung Hee Lee,Kun Jong Lee,Dai-Eun Sok,Hyoung Chin Kim,Hwan Mook Kim,Mee Ree Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.1

The trypsin inhibitor activities (TIA) of various potato cultivars were evaluated by measuring the inhibition of trypsin inhibitor activity using N-benzoyl-DL-arginine-p-nitroanilide (BAPNA) as substrate. The TIA values of 5 potato cultivars (1.99 to 2.88 mg/g) were significantly different among cultivars (p<0.05). When the TIA values of commercially processed potatoes were determined, no TIA was detected. During cooking, the IT₅₀ (time required to reach 50% inhibition of TIA) values were decreased as heating temperature and time increased. The IT₅₀ of moist heating was estimated to be 0.34 min at 100℃, whereas for deep-fat frying the IT50 was 0.13 min at 180oC and 5.28 min for oven baking at 100℃. The IT50 value of microwave cooking was 0.194 min at medium heat, and which was similar to that of pressure cooking at 120℃ (0.185 min). Moreover, there was a negative relationship between temperature (≥80℃) and IT₅₀values (R2=0.99, p<0.01). The TIA of potato was completely inactivated by moist heating at 100℃within 5 min, whereas the pressure cooking at 120℃ and deep-fat frying at 180℃ within 60 and 30 sec, respectively. Based on our results, deep-fat frying is the most effective cooking method to reduce TIA in potatoes.

Large-Scale Conductive Yarns Based on Twistable Korean Traditional Paper (Hanji) for Supercapacitor Applications: Toward High-Performance Paper Supercapacitors

Heo, Young-Jung,Lee, Ji Won,Son, Yeong-Rae,Lee, Jong-Hoon,Yeo, Chang Su,Lam, Tran Dai,Park, Sang Yoon,Park, Soo-Jin,Sinh, Le Hoang,Shin, Min Kyoon Wiley (John WileySons) 2018 Advanced Energy Materials Vol.8 No.27

Effect of gamma irradiation on Korean traditional multicolored paintwork

Yoon, Minchul,Kim, Dae-Woon,Choi, Jong-il,Chung, Yong-Jae,Kang, Dai-Ill,Hoon Kim, Gwang,Son, Kwang-Tae,Park, Hae-Jun,Lee, Ju-Woon Elsevier 2015 Radiation physics and chemistry Vol.115 No.-

<P><B>Abstract</B></P> <P>Gamma irradiation can destroy fungi and insects involved in the bio-deterioration of organic cultural heritages. However, this irradiation procedure can alter optical and structural properties of historical pigments used in wooden cultural heritage paintings. The crystal structure and color centers of these paintings must be maintained after application of the irradiation procedure. In this study, we investigated the effects of gamma irradiation on Korean traditional multicolored paintwork (Dancheong) for the preservation of wooden cultural heritages. The main pigments in Korean traditional wooden cultural heritages, <I>Sukganju</I> (Hematite; Fe<SUB>2</SUB>O<SUB>3</SUB>), <I>Jangdan</I> (Minium; Pb<SUB>3</SUB>O<SUB>4</SUB>), <I>Whangyun</I> (Crocoite; PbCrO<SUB>4</SUB>), and <I>Jidang</I> (Rutile; TiO<SUB>2</SUB>), were irradiated by gamma radiation at doses of 1, 5, and 20kGy. After irradiation, changes in Commision Internationale d’Eclairage (CIE) color values (<I>L</I>*, <I>a</I>*, <I>b</I>*) were measured using the color difference meter, and their structural changes were analyzed using X-ray diffraction (XRD) analysis. The slightly change in less than 1 <I>dE</I>* unit by gamma irradiation was observed, and structural changes in the Dancheong were stable after exposure to 20kGy gamma irradiation. In addition, gamma irradiation could be applied to painted wooden cultural properties from the Korean Temple. Based on the color values, gamma irradiation of 20kGy did not affect the Dancheong and stability was maintained for five months. In addition, the fungicidal and insecticidal effect by less than 5kGy gamma irradiation was conformed. Therefore, the optical and structural properties of Dancheong were maintained after gamma irradiation, which suggested that gamma irradiation can be used for the preservation of wooden cultural heritages painted with Dancheong.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of gamma irradiation on the Dancheong were evaluated. </LI> <LI> We confirmed that optical and structural properties of Dancheong were maintained. </LI> <LI> Irradiation can contribute the decontamination for wooden cultural heritages. </LI> <LI> It also can be used for preservation of painted-wooden cultural heritages. </LI> </UL> </P>

Supercapacitors: Large-Scale Conductive Yarns Based on Twistable Korean Traditional Paper (Hanji) for Supercapacitor Applications: Toward High-Performance Paper Supercapacitors (Adv. Energy Mater. 27/2018)

Heo, Young-Jung,Lee, Ji Won,Son, Yeong-Rae,Lee, Jong-Hoon,Yeo, Chang Su,Lam, Tran Dai,Park, Sang Yoon,Park, Soo-Jin,Sinh, Le Hoang,Shin, Min Kyoon Wiley (John WileySons) 2018 ADVANCED ENERGY MATERIALS Vol.8 No.27

효소에 의한 양파가수분해액의 제조

서형주(Hyung-Joo Suh),정수현(Soo-Hyun Chung),손종연(Jong-Youn Son),손흥수(Hung-Soo Son),조원대(Won-Dai Cho),마상조(Sang-Jo Ma) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.5

본 실험에서는 국내산 양파들의 일반성분, 유리아미노산 및 유리당의 함량을 비교, 분석하였으며, 또한 Celluclast 1.5L, Viscozyme L, Cereflo 및 Pectinex 등의 효소를 첨가하였을 때의 양파 가수분해액의 수율 및 성분 변화에 대하여 조사하고자 하였다. 국내산 양파의 수분, 단백질, 지방, 탄수화물 및 회분의 함량은 89~90%, 1.8~7.9%, 0.4~0.5%, 7.1~8.5% 및 0.3~0.5%인 것으로 나타났다. 주요 유리당은 glucose, fructose 및 sucrose이었으며, 주요 유리아미노산은 cysteine, histidine, arginine 및 glutamic acid였다. Cereflo, Celluclast 1.5L, Pectinex와 Viscozyme L 처리에 의한 양파의 가수분해도는 각각 69%, 71%, 80% 및 62%이었다. 효소에 의한 양파의 가수분해시 유리당의 함량은 증가한 반면 pyruvic acid 함량은 감소하는 경향을 보였다. The changes in chemical constituents of onion in Korea and the degree of hydrolysis in onion hydrolysates by treatment of viscozyme L, celluclast 1.5L, pectinex and cereflo were investigated. Proximate compositions of onion in Korea were as follows; The contents of moisture, protein, lipid, carbohydrate and ash were 89~90%, 1.8~1.9%, 0.4~0.5%, 7.1~8.5% and 0.3~0.5%, respectively. Major free sugars were identified to be glucose, fructose and sucrose and major free amino acids were as cysteine, histidine, arginine and glutamic acid. The degree of hydrolysis by treatment of viscozyme L, celluclast 1.5L, pectinex and cereflo was 69%, 71%, 80% and 62%, respectively. During the enzymatic hydrolysis, free sugar and total sugar content increased however, pyruvic acid content decreased significantly.