소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구

송만용,이삼선,이진구,이원진,허민석,이재일,민병무,최순철 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods : Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results : Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p.0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.

1년간의 자발적인 운동참여가 중년 남성 제2형 당뇨병 환자의 혈중 지질 성분에 미치는 영향

허만동(Man Dong Huh),이진훈(Jin Hoon Lee),류승필(Sung Pil Ryu) 한국발육발달학회 2006 한국발육발달학회지 Vol.14 No.3

The purpose of this study is to investigate the effects of voluntary exercise participation on blood lipids in type II diabetes mellitus middle-aged men. Eighteen male volunteers were recruited and exercised, jogging, cycle ergometer, swimming, in the sports center for 12 months without any supervision. They had educated heart rate calculation method to keep their target heart rate during exercise. Before and after the training, their body composition, maximal oxygen uptake, and blood lipids profiles were analyzed. Body weight was not changed during experimental periods; however body fat ratio was statistically decreased at the same periods. Blood glucose, TC, LDL-C, and TG were decreased significantly and HDL-C was significantly increased (p<.05). RQ was significantly lower compared to the before exercise and their maximal oxygen consumption and exercise time to fatigue were increased significantly (p<.05). These results suggested that voluntary exercise with simple exercise prescription was also effectual to enhance blood lipids in type II diabetes mellitus middle-aged men.

건진센터 수검자 자료를 이용한 비만지표에 대한 연구

김상만,최희정,이득주,김광민,윤수진 대한비만학회 1997 The Korean journal of obesity Vol.6 No.2

We have examined l5145 persons(7468 men 7677 women) to determine the role of Obesity Index(body mass index, WHR, body fat percent) on the risk of various chronic disease. All the subjects were pooled from Health Examination Center who took the annual health examination from June, 1994 through Jan, 1997, BMI(Body mass index, kg/㎡), WHR(Waist hip ratio) were measured and correlated with the frequency of chronic disease. In order to evaluated the BMI, WHR, Body Fat as an independent factors on rnorbidity, analysis of covariance was applied adjusted for age. The risk of variows chronic diseases were also analyzed with obesity, defined by BMI above 26kg/㎡ (men), 25kg/㎡ (women), by logistic regression adjusted age and sex. Risk of hypertension was 2.6 with 95% eonfidence interval of 1.9-2.8 in obese group. Risk of diabetes mel1itus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.2(2.3-4.4), 1.2(0.9-1.4), 2.1(1.7-2.8), 1.4(1.1-2.6) and 1.3(0.8-2.3) respectiveiy. The risk of various chronic diseases were also analyzed with obesity, WHR above 0.9(men), 0.81(women), by logistic regression adjusted age and sex. Risk of hypertension was 1.7 with 95% confidence interval of 1.4 -2.1 in obese group. Risk of diabetes mellitus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.1(2.3-4.0), 1.2(0.9-l.4), 1.4(1.1-2.6), 2.4(l.8-4.8) and 2.1(1.2-4.1) respectively. The BMI, WHR were independent risk factors for various chronic diseases.

한국산 나비목(Lepidoptera)의 발향린(Androconia)에 관한 주사 전자현미경적 연구 Ⅰ. 뱀눈나비과

이종욱,차진열,류성만 嶺南大學校附設 基礎科學硏究所 1988 基礎科學硏究 Vol.8 No.-

한국산 Satyridae 에서 보고된 35종 중 북한산 11종과 채집하지 못한 3종을 제외한 21종을 재료로 광학현미경으로 발향린의 유무 및 분포를 확인하였다. 이중 발향린이 관찰된 9종을 대상으로 주사전자현미경으로 그 형태를 비교 연구하였다. 1. 발향린이 관찰된 종은 Coenonympha ammaryllis Cramer, Erebia wanga Bremer, Eumenis antonoe Esper, Lopinga achine Scopoli, Minois dryas Scopoli, Pararge deidamia Eversmann, Ypthima argus Buttler, Ypthima amphithea Menetries, Ypthima mojschulsk Bremer et Grey 9종 이었다. 2. 발향린은 암컷에서는 발견되지 않았고 수컷의 앞·뒷날개 표면(dorsal surface)에서 관찰되었다. 3. 발향린의 분포는 주로 앞날개의 둔맥과 경맥사이, 뒷날개는 주맥을 중심으로 분포하였으며, 종에 따라서 상당한 차이를 보였다. 4. Satyridae 9종의 발향린은 나발막(fimbriae), neck, 잎새(laminar), 자루병반(foot stalk)등을 갖추었으나, 부속반(accessory disk)은 가지지 않았다. 5. Satyridae 9종의 발향린은 같은 종 내의 다른 개체간, 부위에 따른 변이는 관찰되지 않았다. 6. 발향린의 성유인 물질의 분비는 샘(gland)의 생성물이 자루병반의 강(cavity)내로 들어가서 잎새(laminar), neck, 나발막(fimbrae)의 말단개구부등 발향린 전면에 걸쳐있는 구멍(pore)을 통해 분산되는 것으로 여겨진다. This paper observed through microscope the existance and the distribution of the androconia of the 21 species of Satyride among 35 ones which have been reported in Korea, excluding 11 species in north Korea and 3 one's not in collection. 9 species which were observed the androconia among these were chifely compared and studied through Scanning Electron Microscope. 1. The species observing androconia are ? Coenonympha ammaryllis Cramer, Erebia wanga Bremer, Eumenis antonoe Esper, Lopinga achine Scopoli, Minois dryas Scopoli, Pararge deidamia Eversmann, Ypthima argus Buttler, Ypthima amphithea ? Menetries, Ypthima mojschulsk Bremer et Grey. 2. The androconia was not found at the females, but only at the dorsal surface of the fore and hind wings of males. 3. The androconia was mainly distributed between the anal vein and the radius of the fore wings and at the cubitus of the hind wings, and made differences among the species. 4. The androconia of 9 species Satyridae fimbriae, nack, laminar,foot stalk, but no accessory disk. 5. The androconia of 9 species Satyridae was observed no variation at the sections and among the inviduals of the same species. 6. The secretion of the sexual attractant at the androcnia was through to be diffused into the pores at the surface of the androconia of laminar, neck, and fimbriae through the cavirty of the foot stalk of the gland.

Mixer-settler를 이용한 용매추출로 에칭폐액로부터 철과 니켈의 분리

이만승,이경주,이진영,안재우,오영주 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.12

Solvent extraction and stripping experiments were conducted to separate iron from a spent FeC13 etching solution containing nickel. In the operation of mixer-settler, 7 extraction stages with 1.0 M Alamine336 in toluene, led to 98% extraction percentage of iron at equal phase ratio, when the flow rate of organic and aqueous were 35 mL/min and 5 mL/min, respectively. At equal phase ratio of aqueous to organic, 10 stripping stages with 0.01 M HC1 solution resulted in 99% stripping percentage of iron when the flow rate of stripping solution and loaded organic were 5 mL/min and 35 mL/min, respectively. The concentration of iron in the stripped solution was 133 g/L and this solution could be reused in the etching of nickel lead frame. (Received June 15, 2004)

식용 버섯과 약용버섯의 기능성 식품으로서의 전망

이진만,이상한 東國專門大學 1999 金龜論叢 Vol.7 No.1

최근 버섯은 식용으로서 뿐만 아니라 약용으로서의 기능이 있는 중요한 식품재료로 부각되었다. 우리는 여기에서 최근 식용으로 많이 사용하고 있는 송이, 양송이, 표고, 느타리버섯과, 약용으로서 인식된 아가리쿠스, 상황, 영지, 동충하초에 대하여 이들의 식품으로서의 특수한 기능에 대하여 소개한다. 특히 아직까지 체계적으로 정립되지 않은 이들 약용 버섯 또는 이들 버섯성분의 기능을 보다 더 체계적으로 연구하기 위한 예비단계로서, 여러 가지 항암활성 assay법을 서술하고자 한다. 버섯내의 특이성분에 대한 분자의학적인 mechanism이 밝혀진다면, 암 및 다른 성인병의 치료적인 면에서 버섯류의 역할이 중요하게 인식될 것이다. 미래의 농가소득의 증대와 국민의 보건향상의 측면에서도 이들 버섯산업 및 이와 관련된 의약품개발을 매우 활성화시켜야 하겠다.

천마(天麻, Gastrodia elata Blume) 종균배양의 최적화에 대한 연구

이진만,김성호 東國專門大學 2002 金龜論叢 Vol.9 No.1

천마는 뽕나무버섯과 공생관계로 버섯에 의한 영양공급에 의해서만 생육이 가능한 것으로 알려져 있다. 따라서 천마의 다수확재배를 위한 가장 중요한 인자중의 하나로 우수한 품질의 종균배양은 천마의 재배지의 활착율에 큰 영향을 미치므로 생육 및 수확량, 재배기간 등과 직결된다고 생각된다. 한편 5-6년 전부터 경북 칠곡군 관내에서 대량인공 재배되기 시작하여 그 재배방법에 대한 기초연구가 절실한 실정이다. 따라서 천마재배에 대한 기초연구로서 천마의 종균배양의 최적화를 통해 천마의 다수확재배법의 구축을 위한 기초연구를 수행한 결과, 종균배양에 있어서 액체배양시 물에 용해될 때 친수기 부분이 이온으로 해리되지 않는 계면활성제인 Tween80의 첨가량을 0.0066%, 5mm glass bead ball 30개를 첨가하여 배양시 pulpy growth를 유도할 수 있었으며, 균사체의 질과 양적인 면에서 가장 우수하였다. 따라서 상기의 조건에서 배양한 종균을 참나무 톱밥배지에 5%접종하여 45일간 종균 배양후 25℃, 60%의 습도조건에서 참나무 활착도가 가장 우수하였다.

效率的인 下水處理 改善方案에 關한 硏究

이진희,류제천,김송만,박춘수 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

Greeting the local self-government age, local finance independece rate of local autonomy is on very poor state. So, the method of discharge a cleanest water with the least expense in sewage treatment is an important factor to supply clean water to Chung-Nam, Chung-Buk and Daejoen and to preserve a water quality of Daechong Lake. Therefore, in this study, we analysed not only a facilities outline of environment office and operating state for improvement of a water quality but also investment cost, maintenance cost, reduction method of working expenses for reduction of a budget. As a result of study, it is concluded to improve sewage pipe line of separate type and to install a flow control tank and to introduce a remove technique of T-N for the purpose of water quality improvement. Also, the relation of F/M ratio and outflow quality does not exist a regular proportional expression. But, water is the cleanest condition when the F/M ratio is 0.04. And, the most suitable injection rate of chemical material was 20㎎/ℓ for Al₂(SO₄)₃(8%) and 50㎎/ℓ(0.1%) for Polymer(0.1%). As a reduction method of operation cost, it is necessary to manage a technique support team and manage a foul water plant under township by environment office. As a result of comparison of an electric fee, a chemical cost, a by-produce recycle, and a facilities improvement, total expenses was reduced about 9% in 1996.

비누화 PC88A에 의한 NdCl₃와 SmCl₃용매추출의 화학모델

이만승,이광섭,이진영,김성돈,김준수 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.10

A chemical model was developed to estimate equilibrium constant for the solvent extraction of Nd and Sm from chloride solution with saponified PC88A. The model could be employed to predict the distribution coefficient of metal from the initial extraction conditions. The model consisted of chemical equilibria, mass balance, charge balance and solvent extraction reaction. By applying the model to the experimental conditions and data, equilibrium constants for the solvent extraction of Nd and Sm were obtained. The effect of the saponification of PC88A on the extraction of Nd was explained in terms of the equilibrium constant obtained in this study and the change of equilibrium pH. The predicted distribution coefficients of Nd and Sm agreed well with those measured. (Received July 14, 2004)