Use of Long-chain Alcohol in Extraction and Purification of Lincomycin from Fermentation Broth

Wu, Bin,Zhu, Jiawen,Chen, Kui,Ji, Lijun,Guo, Jianguo,Zhao, Jianxi 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.2

Low selectivity for lincomycin in butanol extraction process leads to relatively higher content of impurities. A novel process for extraction of lincomycin from fermentation broth was studied in this work. Mixture of n-octanol and n-decanol is used as extractant to replace n-butanol in extraction of lincomycin. Oprimal operation conditions for the process have been studied. Due to higher extraction selectivity for lincomycin A by long-chain alcohol, content of impurity(lincomycin B) in the final product is much lower than that in product by butanol process. Furthemore, the practicability for combination of long-chain alcohol and butanol in purification of linecomycin was investigated.

An experimental study on low-velocity nonlinear flow in vuggy carbonate reservoirs

Haitao Wu,Fujun Li,Jiawen Liu,Jiang Chen,Xiaowei Lv,Wei Hu 한국자원공학회 2016 Geosystem engineering Vol.19 No.4

Vuggy carbonate reservoirs in the Middle East, which have characteristics such as high porosity, low permeability, and few fractures, are significantly different from fractured-vuggy carbonate reservoirs. In this study, single-phase flow experiments and core mercury injection experiments were conducted to analyze the pore size distribution and low-velocity nonlinear flow characteristics of vuggy carbonate rocks in Halfaya oilfield. The results show that the relationship between pseudo-threshold pressure gradient and permeability in matrix carbonate rocks is linear. However, vuggy type does not exhibit the same linear relationship. Compared with matrix type, vuggy carbonate rock has a nonlinear flow zone when the pressure gradient is comparatively low and the nonlinear flow characteristics are quite different from those of low-permeability sandstone reservoirs. Because of the multi-polarization of flow channel radius in vuggy carbonate rocks, fluid stepwise starts flowing as the pressure gradient increases. Vuggy carbonate rock has a pseudo-threshold pressure gradient during the low-velocity flow, but the actual threshold pressure gradient does not exist. The concept of whole channel flow pressure gradient is presented in this paper. The results contribute to improve the understanding of low-velocity flow characteristics of vuggy carbonate reservoirs.

Reactive extraction of 2,3-butanediol from fermentation broth

Yanyang Wu,Yanjun Li,Jiawen Zhu,Jiaxian Liu 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

Biochemical 2,3-butanediol is a renewable material, but the lack of an effective separation process limits its industrial application. We developed an effective separation process to recover 2,3-butanediol from fermentation broth by reactive-extraction with ion-exchange resin HZ732 as catalyst. n-Butylaldehyde was used as both reactant and extractant. Feasible operation conditions were obtained as follows: room temperature, Ccat=200 g·L−1, three-stage cross-current extraction, with reactant ratio (VButylaldehyde : Vfermentation broth) 0.05 for each stage. Reactive-extraction can recover over 98% of 2,3-butanediol in the form of 2-propyl-4,5-dimethyl-1,3-dioxolane from fermentation broth. Then 2,3-butanediol was obtained by hydrolyzing 2-propyl-4,5-dimethyl-1,3-dioxolane and purified by vacuum distillation. The total yield rate of 2,3-butanediol through the process was over 94% and purity of final product reached 99%.

Reactive-extraction of 2,3-butanediol from fermentation broth by propionaldehyde: Equilibrium and kinetic study

Yanyang Wu,Yanjun Li,Jiawen Zhu,Jiaxian Liu 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

An effective process was developed to separate 2,3-butanediol (2,3-BD) from fermentation broth (FB)by reactive-extraction. Propionaldehyde (PRA) was used as reactant and reaction product 2-ethyl-4,5-dimethyl-1,3-dioxolane (EDD) acted as extractant. HCl was selected as catalyst. Appropriate conditions were obtained by experiment as follows: 10 oC, CHCl=0.2mol·L−1, two-stage cross-current extraction, reactant volume ratio (VPRA : VFB) for first stage and second stage is 0.10 and 0.05, respectively. The yield rate of 2,3-butanediol for the whole process can reach 90%w/w, and 2,3-butanediol in the final product can be more than 99% w/w. The novel process required less solution and especially had advantages in treating dilute fermentation broth. Furthermore, equilibrium and kinetic study were investigated on the reaction of propionaldehyde and 2,3-butanediol to provide basic data for process development. The results reveal that reaction enthalpy and activation energy of the reaction were −21.84±2.38 KJ·mol−1 and 51.97±2.84 KJ·mol−1, respectively. Kinetics was well described by pseudo-homogeneous model.

Atmospheric Pressure Air Plasma Treatment to Improve Dyeability with Cortex Phellodendri Plant Powder Dye: An Ecofriendly and Energy-Efficient Approach for Silk Processing

Fei Fan,Xunxin Wu,Jiaxiang Wen,Gengyu Lin,Jiawen Lin,Qiuting Yin 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

This paper explores the effects of a dielectric barrier discharge atmospheric pressure air plasma and citric acid (CA) pretreatment on dyeing gray mulberry silk plain fabrics with a cortex phellodendri plant powder dyestuff. The atmospheric air plasma treatment formed new hydrophilic groups, such as C-O, O-C=O, and C-NH2, on the surface of the fabric. The relative contents of the C-O (C-N) and O-C=O groups indicated that the efficiency of bonding the hydrophilic groups to the fabric increased in the order of citric acid alone < collaborative treatment in different orders < original < atmospheric air plasma alone. X-ray photoelectron spectroscopy (XPS) showed that the relative content of C-O (C-N)/C=O was the highest after 9 treatments at 360 W. Erosion pits and grooves with widths of 0.5-3 µm appeared on the surface after plasma treatment. The surface of the silk fabric was negatively charged, and it interacted electrostatically, adsorbed and combined with the cationic dye. The samples were dyed at 60 °C, with a pH 8, a 60% (owf) dye concentration, a bath ratio of 1:40, and a 60 min dyeing time. After soaping, the K/S value had increased from 8.05 to 13.51, and the washing color fastness rating was 4-5.

Microstructure and Compression Strength of W/HfC Composites Synthesized by Plasma Activated Sintering

Jian Zhang,Shuai Ma,Jiawen Zhu,Kejia Kang,Guoqiang Luo,Chuandong Wu,Qiang Shen,Lianmeng Zhang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.2

In this work, W/HfC composite materials were synthesized using plasma activated sintering. The influence of the sinteringtemperature and HfC weight fraction on the relative density, microstructure and compression strength were investigated. The results demonstrated that the sintering temperature and the HfC content significantly affected the microstructure of W/HfC composites. Moreover, the grain size of the W/HC composites decreased and the mechanical properties were improvedremarkably due to the addition of HfC. The majority of HfC particles reacted with oxygen impurities to generate HfO2,whichpurified the grain boundaries and refined the grain size of the W matrix. The optimum content of HfC is 2 wt%, at which ahigh compressive strength of 1.98 GPa and a high strain of 34.7% were obtained.

Separation of 2,3-Butanediol from Fermentation Broth by Reactiveextraction Using Acetaldehyde-cyclohexane System

Yanjun Li,Yanyang Wu,Jiawen Zhu,Jiaxian Liu 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2

Biochemical 2,3-butanediol is a renewable material with the potential to be used as an alternative fuel. However, in the lack of an effective separation process has limited its industrial application. In this paper, an effective process was achieved to separate 2,3-butanediol by reactive-extraction. Acetaldehyde and cyclohexane were chosen as the reactant and extractant, respectively. Ion-exchange resin HZ732 was used as the catalyst. Reaction equilibrium and a kinetic study on the reaction between 2,3-butanediol and acetaldehyde were investigated to provide basic data for process development. The reaction enthalpy and activation energy of reaction of 2,3-butanediol and acetaldehyde were −30.05 ± 1.62 KJ/mol and 45.29 ± 2.89 KJ/mol,respectively. Feasible conditions were obtained as follows:operating temperature = 20℃, acetaldehyde: 2,3-butanediol = 0.5:1 (w/w), cyclohexane: fermentation broth = 0.5:1 (w/w), catalyst amount = 100 g/L, stirring rate = 500 rpm and three-stage counter-current extraction method was used. Under these conditions, the total yield rate of 2,3-butanediol from fermentation broth was over 90% and the mass fraction of 2,3-butanediol in the final product reached 99%. Biochemical 2,3-butanediol is a renewable material with the potential to be used as an alternative fuel. However, in the lack of an effective separation process has limited its industrial application. In this paper, an effective process was achieved to separate 2,3-butanediol by reactive-extraction. Acetaldehyde and cyclohexane were chosen as the reactant and extractant, respectively. Ion-exchange resin HZ732 was used as the catalyst. Reaction equilibrium and a kinetic study on the reaction between 2,3-butanediol and acetaldehyde were investigated to provide basic data for process development. The reaction enthalpy and activation energy of reaction of 2,3-butanediol and acetaldehyde were −30.05 ± 1.62 KJ/mol and 45.29 ± 2.89 KJ/mol,respectively. Feasible conditions were obtained as follows:operating temperature = 20℃, acetaldehyde: 2,3-butanediol = 0.5:1 (w/w), cyclohexane: fermentation broth = 0.5:1 (w/w), catalyst amount = 100 g/L, stirring rate = 500 rpm and three-stage counter-current extraction method was used. Under these conditions, the total yield rate of 2,3-butanediol from fermentation broth was over 90% and the mass fraction of 2,3-butanediol in the final product reached 99%.

Experimental study and modeling of residence time distribution in impinging stream reactor with GDB model

Lijun Ji,Kui Chen,Jiawen Zhu,Bin Wu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.4

Residence time distribution (RTD) in an impinging streams reactor with two or four nozzles was investigated with KCl solution as a tracer. The results showed that the flow pattern in the reactor was close to that in a continuous stirred tank reactor (CSTR). Macromixing process in the reactor was improved obviously when the opposite nozzles were added. Based on the analysis of flow region in the reactor, gamma distributionmodel with bypass (GDB) was applied for study on the RTD of the reactor. It was found that RTD in the impinging streams reactor could be finely described by the model. Also the effects of experimental conditions on parameters of model were analyzed according to the correlated values of the model parameters.

Simulation of protein adsorption in a batchwise affinity chromatography with a modified rate model

Mohammad Reza Aboudzadeh,Zhu Jiawen,Wu Bin 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.6

Inhibition of Fatty Acid β-Oxidation by Fatty Acid Binding Protein 4 Induces Ferroptosis in HK2 Cells Under High Glucose Conditions

Zhihua Zheng,Jiasi Chen,Keping Wu,Yan Lei,Mingcheng Huang,Lokyu Cheng,Hui Guan,Jiawen Lin,Ming Zhong,Xiaohua Wang 대한내분비학회 2023 Endocrinology and metabolism Vol.38 No.2

Background: Ferroptosis, which is caused by an iron-dependent accumulation of lipid hydroperoxides, is a type of cell death linked todiabetic kidney disease (DKD). Previous research has shown that fatty acid binding protein 4 (FABP4) is involved in the regulation of ferroptosis in diabetic retinopathy. The present study was constructed to explore the role of FABP4 in the regulation of ferroptosis in DKD. Methods: We first detected the expression of FABP4 and proteins related to ferroptosis in renal biopsies of patients with DKD. Then, we used a FABP4 inhibitor and small interfering RNA to investigate the role of FABP4 in ferroptosis induced by high glucosein human renal proximal tubular epithelial (HG-HK2) cells. Results: In kidney biopsies of DKD patients, the expression of FABP4 was elevated, whereas carnitine palmitoyltransferase-1A (CPT1A), glutathione peroxidase 4, ferritin heavy chain, and ferritin light chain showed reduced expression. In HG-HK2 cells, the induction of ferroptosis was accompanied by an increase in FABP4. Inhibition of FABP4 in HG-HK2 cells changed the redox state, suppressing the production of reactive oxygen species, ferrous iron (Fe2+), and malondialdehyde, increasing superoxide dismutase, and reversing ferroptosis-associated mitochondrial damage. The inhibition of FABP4 also increased the expression of CPT1A, reversed lipiddeposition, and restored impaired fatty acid β-oxidation. In addition, the inhibition of CPT1A could induce ferroptosis in HK2 cells. Conclusion: Our results suggest that FABP4 mediates ferroptosis in HG-HK2 cells by inhibiting fatty acid β-oxidation.