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Hua, Jianming,Shen, Ning,Wang, Jingkai,Tao, Yiqing,Li, Fangcai,Chen, Qixin,Zhou, Xiaopeng Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.9
Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising for regenerating degenerated intervertebral discs (IVDs), but the low efficiency of nucleus pulposus (NP)-specific differentiation limits their clinical applications. The Sonic hedgehog (Shh) signaling pathway is important in NP-specific differentiation of ADSCs, and Smoothened Agonist (SAG) is a highly specific and effective agonist of Shh signaling. In this study, we proposed a new differentiation strategy with the use of the small molecule SAG. The NP-specific differentiation and extracellular matrix (ECM) synthesis of ADSCs were measured in vitro, and the regenerative effects of SAG pretreated ADSCs in degenerated IVDs were verified in vivo. The results showed that the combination of SAG and transforming growth factor-${\beta}3$ ($TGF-{\beta}3$) is able to increase the ECM synthesis of ADSCs. In addition, the gene and protein expression levels of NP-specific markers were increased by treatment with SAG and $TGF-{\beta}3$. Furthermore, SAG pretreated ADSCs can also improve the disc height, water content, ECM content, and structure of degenerated IVDs in vivo. Our new differentiation scheme has high efficiency in inducing NP-specific differentiation of ADSCs and is promising for stem cell-based treatment of degenerated IVDs.
Jianming Hua,Ning Shen,Jingkai Wang,Yiqing Tao,Fangcai Li,Qixin Chen,Xiaopeng Zhou 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.9
Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising for regenerating degenerated intervertebral discs (IVDs), but the low efficiency of nucleus pulposus (NP)-specific differentiation limits their clinical applications. The Sonic hedgehog (Shh) signaling pathway is important in NP-specific differentiation of ADSCs, and Smoothened Agonist (SAG) is a highly specific and effective agonist of Shh signaling. In this study, we proposed a new differentiation strategy with the use of the small molecule SAG. The NP-specific differentiation and extracellular matrix (ECM) synthesis of ADSCs were measured in vitro, and the regenerative effects of SAG pretreated ADSCs in degenerated IVDs were verified in vivo. The results showed that the combination of SAG and transforming growth factor-β3 (TGF-β3) is able to increase the ECM synthesis of ADSCs. In addition, the gene and protein expression levels of NP-specific markers were increased by treatment with SAG and TGF-β3. Furthermore, SAG pretreated ADSCs can also improve the disc height, water content, ECM content, and structure of degenerated IVDs in vivo. Our new differentiation scheme has high efficiency in inducing NP-specific differentiation of ADSCs and is promising for stem cell-based treatment of degenerated IVDs.
Soft Hemirings Related to Fuzzy Set Theory
Wu, Hua-Jun,Zhan, Jianming Department of Mathematics 2012 Kyungpook mathematical journal Vol.52 No.1
In this paper, we investigate soft hemirings by fuzzy theory. Some characterizations of hemirings are introduced by means of soft sets. In particular, the $h$-hemiregular hemirings and $h$-intra-hemiregular hemirings are also characterized.
Characterization and mapping of a Prbs gene controlling spike development in Hordeum vulgare L.
Yi Shang,Jianming Yang,Jinghuan Zhu,Wei Hua,Junmei Wang,Qiaojun Jia 한국유전학회 2014 Genes & Genomics Vol.36 No.3
The barley mutant, poly-row-and-branchedspike (prbs) showed altered inflorescence morphology:complete conversion of the rudimentary lateral spikelets intwo-rowed barley into fully developed fertile spikeletssimilar to the six-rowed phenotype, and additional spikeletsin the middle of spike. Moreover, branched spikes emergedin progeny from a cross between the mutant and a sixrowedbarley cultivar. Morphological observation of thedevelopment of immature spikes of the mutant anddescendants with branched spikes showed that the Prbsgene is involved in spikelet development in the triplemoundstage. In mutant prbs, new meristems initiated atthe flanks of lateral spikelets and middle spikelet meristemswere converted to branch meristems, developing branchedspikes. These observations suggested that the Prbs geneplays a crucial role in spikelet initiation and identitymaintenance. The Prbs gene may be an important modifierin inflorescence differentiation from a panicle into a spike. The branched spikes emerging in hybrids from a crossbetween the mutant and six-rowed barley cultivar were notconferred by the gene vrs1 or Int-c, which decide spikemorphology in six-rowed barley. These results imply thatalthough six-row genes vrs1 and Int-c and prbs have similareffects on lateral spikelet development, they have differentfunctions in branched spikes. The Prbs gene wasmapped to chromosome 3H between SSR markerBmag0023 and marker Cbic60 at a genetic distance of 3.3and 5.4 centimorgans (cM), respectively.