DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Seismic behavior of circular-in-square concrete-filled high-strength double skin steel tubular stub columns with out-of-code B/t ratios

Jian-Tao Wang,Yue Wei,Juan Wang,Yu-Wei Li,Qing Sun 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.49 No.4

Aiming at the development trend of light weight and high strength of engineering structures, this paper experimentally investigated the seismic performance of circular-in-square high-strength concrete-filled double skin steel tubular (HCFDST) stub columns with out-of-code width-to-thickness (B/t) ratios. Typical failure mode of HCFDST stub columns appeared with the infill material crushing, steel fracture and local buckling of outer tubes as well as the inner buckling of inner tubes. Subsequently, the detailed analysis on hysteretic curves, skeleton curves and ductility, energy dissipation, stiffness degradation and lateral force reduction was conducted to reflect the influences of hollow ratios, axial compression ratios and infill types, e.g., increasing hollow ratio from 0.54 to 0.68 and 0.82 made a slight effect on bearing capacity compared to the ductility coefficients; the higher axial compression ratio (e.g., 0.3 versus 0.1) significantly reduced the average bearing capacity and ductility; the HCFDST column SCFST-6 filled with concrete obviously displayed the larger initial secant stiffness with a percentage 34.20% than the column SCFST-2 using engineered cementitious composite (ECC); increasing hollow ratios, axial compression ratios could accelerate the drop speed of stiffness degradation. The out-of-code HCFDST stub columns with reasonable design could behave favorable hysteretic performance. A theoretical model considering the tensile strength effect of ECC was thereafter established and verified to predict the moment-resisting capacity of HCFDST columns using ECC. The reported research on circular-in-square HCFDST stub columns can provide significant references to the structural application and design.

Channel and Gate Workfunction-Engineered CNTFETs for Low-Power and High-Speed Logic and Memory Applications

Wang, Wei,Xu, Hongsong,Huang, Zhicheng,Zhang, Lu,Wang, Huan,Jiang, Sitao,Xu, Min,Gao, Jian The Institute of Electronics and Information Engin 2016 Journal of semiconductor technology and science Vol.16 No.1

Carbon Nanotube Field-Effect Transistors (CNTFETs) have been studied as candidates for post Si CMOS owing to the better electrostatic control and high mobility. To enhance the immunity against short - channel effects (SCEs), the novel channel and gate engineered architectures have been proposed to improve CNTFETs performance. This work presents a comprehensive study of the influence of channel and gate engineering on the CNTFET switching, high frequency and circuit level performance of carbon nanotube field-effect transistors (CNTFETs). At device level, the effects of channel and gate engineering on the switching and high frequency characteristics for CNTFET have been theoretically investigated by using a quantum kinetic model. This model is based on two-dimensional non-equilibrium Green's functions (NEGF) solved self - consistently with Poisson's equations. It is revealed that hetero - material - gate and lightly doped drain and source CNTFET (HMG - LDDS - CNTFET) structure can significantly reduce leakage current, enhance control ability of the gate on channel, improve the switching speed, and is more suitable for use in low power, high frequency circuits. At circuit level, using the HSPICE with look - up table(LUT) based Verilog - A models, the impact of the channel and gate engineering on basic digital circuits (inverter, static random access memory cell) have been investigated systematically. The performance parameters of circuits have been calculated and the optimum metal gate workfunction combinations of ${\Phi}_{M1}/{\Phi}_{M2}$ have been concluded in terms of power consumption, average delay, stability, energy consumption and power - delay product (PDP). In addition, we discuss and compare the CNTFET-based circuit designs of various logic gates, including ternary and binary logic. Simulation results indicate that LDDS - HMG - CNTFET circuits with ternary logic gate design have significantly better performance in comparison with other structures.

Channel and Gate Workfunction-Engineered CNTFETs for Low-Power and High-Speed Logic and Memory Applications

Wei Wang,Hongsong Xu,Zhicheng Huang,Lu Zhang,Huan Wang,Sitao Jiang,Min Xu,Jian Gao 대한전자공학회 2016 Journal of semiconductor technology and science Vol.16 No.1

Carbon Nanotube Field-Effect Transistors (CNTFETs) have been studied as candidates for post Si CMOS owing to the better electrostatic control and high mobility. To enhance the immunity against short - channel effects (SCEs), the novel channel and gate engineered architectures have been proposed to improve CNTFETs performance. This work presents a comprehensive study of the influence of channel and gate engineering on the CNTFET switching, high frequency and circuit level performance of carbon nanotube field-effect transistors (CNTFETs). At device level, the effects of channel and gate engineering on the switching and high frequency characteristics for CNTFET have been theoretically investigated by using a quantum kinetic model. This model is based on two-dimensional non-equilibrium Green’s functions (NEGF) solved self - consistently with Poisson’s equations. It is revealed that hetero -material - gate and lightly doped drain and source CNTFET (HMG - LDDS - CNTFET) structure can significantly reduce leakage current, enhance control ability of the gate on channel, improve the switching speed, and is more suitable for use in low power, high frequency circuits. At circuit level, using the HSPICE with look - up table(LUT) based Verilog - A models, the impact of the channel and gate engineering on basic digital circuits (inverter, static random access memory cell) have been investigated systematically. The performance parameters of circuits have been calculated and the optimum metal gate workfunction combinations of ФM1/ФM2 have been concluded in terms of power consumption, average delay, stability, energy consumption and power - delay product (PDP). In addition, we discuss and compare the CNTFET-based circuit designs of various logic gates, including ternary and binary logic. Simulation results indicate that LDDS - HMG - CNTFET circuits with ternary logic gate design have significantly better performance in comparison with other structures.

Anti-HIV-1 Activity of Lignans from the Fruits of Schisandra rubriflora

Wei-Lie Xiao,Rui-Rui Wang,Wei Zhao,Ren-Rong Tian,Shan-Zhai Shang,Liu-Meng Yang,Jian-Hong Yang,Jian-Xin Pu,Yong-Tang Zheng,Han-Dong Sun 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.5

This study investigated the 70% aqueous acetone extract of the fruits of Schisandra rubriflora which led to the isolation of eight lignans, including a new isolate, rubrisandrin C (1), and seven known lignans (2-8) . The structure of 1 was established by extensive 1D and 2D NMR spectroscopy and its absolute stereochemistry was determined by CD spectrum. Compounds 1-5 and 7-8 were evaluated for their anti-HIV-1 activity that showed inhibitory activity on HIV-1IIIB induced syncytium formation with EC50 values in the range of 2.26~20.4 μg/mL. Compounds 1 and 7 exerted their obvious protection of HIV-1IIIB inducted MT-4 host cells lytic effects with a selectivity index of 15.4 and 24.6, respectively.

A New Ambiguity Elimination Method for BSS Block Signals in Time Domain

Wei Zhao,Fengshan Wang,Yuehong Shen,Yuanyuan Wu,Zhigang Yuan,Pengcheng Xu,Pengcheng Xu,Yimin Wei,Wei Jian 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.11

This paper deals with the ambiguity problem of blind source separation (BSS) in the case where continuously received mixture signals are split in time and processed block by block. Due to the inherent permutation and scaling ambiguities of BSS, tying the separated components at each adjacent time blocks doesn’t recover the original source signals correctly in general. Inspired by the Permutation Method of reconstructing source signal blocks in time domain, a new ambiguity elimination approach is proposed in this paper. This method aims to concatenate the separated components in adjacent blocks by artificially setting contrast blocks for each adjacent time blocks. The core idea of this method is to utilize the associativity between components recovered from contrast blocks and corresponding adjacent blocks. Compared with Permutation Method, the main advantage of this new method consists in the fact that it is much more efficient in terms of separation quality and computational speed. Besides, a tradeoff can be adjusted between separation quality and computational speed by choosing different length of contrast blocks. Real-life experiments are performed to validate the performance of this method on the wireless communication system with two transmitting and receiving antennas.

The genome of the mesopolyploid crop species Brassica rapa

Wang, Xiaowu,Wang, Hanzhong,Wang, Jun,Sun, Rifei,Wu, Jian,Liu, Shengyi,Bai, Yinqi,Mun, Jeong-Hwan,Bancroft, Ian,Cheng, Feng,Huang, Sanwen,Li, Xixiang,Hua, Wei,Wang, Junyi,Wang, Xiyin,Freeling, Michael Nature Publishing Group, a division of Macmillan P 2011 Nature genetics Vol.43 No.10

We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops.

BCC-DPSO Algorithm for Task Scheduling on NOC

Wei Gao,Yubai Li,Song Chai,Jian Wang 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.5

In this paper, a BCC-DPSO scheduling algorithm is proposed to solve multi-objective optimization problem for task scheduling on Network-on-Chip (NoC). In our proposal, the relative advantage of the solution is evaluated by calculating its efficiency using BCC model in Data Envelopment Analysis (DEA), and the referred-time method is introduced to rank the BCC-efficient solution. Moreover, a sub-swarm strategy is adopted to reduce the high computational requirement introduced by the DEA. There are four sub-swarms, each of which optimizes one of four observed metrics, namely makespan, energy, link load and workload balance. Meanwhile, the speed vector updating formulation is modified to comply with the sub-swarm strategy. By conducting comparative simulations, the results show that our proposal produces more efficient schedule solution than other multi-objective Particle Swarm Optimization (PSO).

Fusion Between TMPRSS2 and ETS Family Members (ERG, ETV1, ETV4) in Prostate Cancers from Northern China

Wang, Jian-Jiang,Liu, Yue-Xin,Wang, Wei,Yan, Wei,Zheng, Yu-Peng,Qiao, Lu-Dong,Liu, Dan,Chen, Shan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

In this study we evaluated the frequency of fusion between TMPRSS2 and ETS family members (ERG, ETV1, ETV4) in prostate cancers in patients from northern China in order to explore differences in fusion rates among regions in northern and southern China, other parts of Asia, Europe, and North America. We examined 100 prostate cancer patients, diagnosed by means of prostate biopsy; fluorescence in situ hybridization (FISH) was used to detect the expression of TMPRSS2, ERG, ETV1 and ETV4 in cancer tissue. Differences in gene fusion rates among different ethnics groups were also analyzed. Of the 100 prostate cancer patients, 55 (55%) had the fusion gene. Among the patients with the fusion gene, 46 (83.6%) patients had the TMPRSS2:ERG fusion product, 8 (14.8%) patients had TMPRSS2:ETV1 fusion, 1 (1.6%) patient had TMPRSS2:ETV4.

A Novel Wireless Statistical Division Multiplexing Communication System and Performance Analysis

Wei Zhao,Yuehong Shen,Pengcheng Xu,Jiangong Wang,Zhigang Yuan,Yimin Wei,Wei Jian,Hui Li 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.5