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Jian Son,Hong-Li Wang,Ke-Han Song,Zhi-Wen Ding,Hai-Lian Wang,Xiao-Sheng Ma,Fei-Zhou Lu,Xin-Lei Xia,Ying-Wei Wang,Fei-Zou,Jian-Yuan Jiang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
This study was carried out to explore the roles of circular RNAs (circRNAs) in nucleus pulposus (NP) tissues in intervertebral disc degeneration (IDD). Differentially expressed circRNAs in IDD and normal NP tissues were identified based on the results of microarray analysis. Bioinformatics techniques were employed to predict the direct interactions of selected circRNAs, microRNAs (miR), and mRNAs. CircRNA_104670 was selected as the target circRNA due to its large multiplier expression in IDD tissues. After luciferase reporter and EGFP/RFP reporter assays, we confirmed that circRNA_104670 directly bound to miR-17-3p, while MMP-2 was the direct target of miR-17-3p. The receiver-operating characteristic (ROC) curve showed that circRNA_104670 and miR-17-3p had good diagnostic significance for IDD (AUC circRNA_104670 = 0.96; AUC miRNA-17-3p = 0.91). A significant correlation was detected between the Pfirrmann grade and expression of circRNA_104670 (r = 0.63; p = 0.00) and miR-17-3p (r = −0.62; p = 0.00). Flow-cytometric analysis and the MTT assay showed that interfering with circRNA_104670 using small interfering RNA (siRNA) inhibited NP cell apoptosis (p < 0.01), and this inhibition was reduced by interfering with miR-17-3p. Interfering with circRNA_104670 suppressed MMP-2 expression and increased extracellular matrix (ECM) formation, which were also reduced by interfering with miR-17-3p. Finally, an MRI evaluation showed that circRNA_104670 inhibition mice had a lower IDD grade compared with control mice (p < 0.01), whereas circRNA_104670 and miRNA-17-3p inhibition mice had a higher IDD grade compared with circRNA_104670 inhibition mice (p < 0.05). CircRNA_104670 is highly expressed in the NP tissues of IDD and acts as a ceRNA during NP degradation.
Son, Hokyoung,Seo, Young-Su,Min, Kyunghun,Park, Ae Ran,Lee, Jungkwan,Jin, Jian-Ming,Lin, Yang,Cao, Peijian,Hong, Sae-Yeon,Kim, Eun-Kyung,Lee, Seung-Ho,Cho, Aram,Lee, Seunghoon,Kim, Myung-Gu,Kim, Yongs Public Library of Science 2011 PLoS pathogens Vol.7 No.10
<▼1><P><I>Fusarium graminearum</I> is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 <I>Fusarium graminearum</I> genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of <I>F. graminearum</I> TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.</P></▼1><▼2><P><B>Author Summary</B></P><P>Large collections of mutant lines allow for identification of gene functions. Here we constructed a mutant library of 657 putative transcription factors (TFs) through homologous recombination in the head blight fungus, <I>Fusarium graminearum</I>, providing a resource for understanding gene regulation in fungus. By screening these mutants in 17 phenotypic categories, we constructed a dataset of over 11,000 phenotypes. This study provides new insight into understanding multiple phenotypes caused by single TF as well as regulation of gene expression at the transcription level in <I>F. graminearum</I>. Furthermore, our TF mutant library will be a valuable resource for fungal studies through the distribution of mutants and easy access to our phenotypic and genetic data.</P></▼2>
Metabolic Flux Analysis of Beijerinckia indica for PS-7 Production
Wu Jian-Rong,Son Jeong Hwa,Seo Hyo-Jin,Kim Ki-Hong,Nam Yoon-Kwon,Lee Jin-Woo,Kim Sung-Koo The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1
In order to investigate central metabolic changes in Beijerinckia indica, cells were grown on different carbon sources and intracellular flux distributions were studied under varying concentrations of nitrogen. Metabolic fluxes were estimated by combining material balances with extracellular substrate uptake rate, biomass formation rate, and exopolysaccharide (EPS) accumulation rate. Thirty-one metabolic reactions and 30 intracellular metabolites were considered for the flux analysis. The results revealed that most of the carbon source was directed into the Entner-Doudoroff pathway, followed by the recycling of triose-3-phosphate back to Hexose6-phosphate. The pentose phosphate pathway was operated at a minimal level to supply the precursors for biomass formation. The different metabolic behaviors under varying nitrogen concentrations were observed with flux analysis.
Wang, Jian-Xun,Hyung, Gun Woo,Li, Zhao-Hui,Son, Sung-Yong,Kwon, Sang Jik,Kim, Young Kwan,Cho, Eou Sik American Scientific Publishers 2012 Journal of Nanoscience and Nanotechnology Vol.12 No.7
<P>In this research, we reported on the fabrication of top-contact amorphous-indium gallium zinc oxide (a-IGZO) thin-film transistors (TFTs) with an organic buffer layer between inorganic gate dielectric and active layer in order to improve the electrical properties of devices. By inserting an organic buffer layer, it was possible to make an affirmation of the improvements in the electrical characteristics of a-IGZO TFTs such as subthreshold slope (SS), on/off current ratio (I(ON/OFF)), off-state current, and saturation field-effect mobility (muFE). The a-IGZO TFTs with the cross-linked polyvinyl alcohol (c-PVA) buffer layer exhibited the pronounced improvements of the muFE (17.4 cm2/Vs), SS (0.9 V/decade), and I(ON/OFF) (8.9 x 10(6)).</P>
김건(Jin Jian),고태조(Tae-Jo Ko),김희술(Hee-Sool Kim),손재환(Jae-Whan Son) 한국기계가공학회 2007 한국기계가공학회 춘추계학술대회 논문집 Vol.2007 No.-
the demand of automobile parts is the development of the automobile industry. The worm shaft manufactured by whirling machine having large drive torque and small noise is very popular in the automobile industry. in Korea most of machining center used to make the worm shaft is using the imported components from German, Japan and other countries. The high price of this equipment will increase the price of the worm shaft. In order to improve the quality of automobile parts and to reduce the cost, it is necessary to import the substitutes of whirling machine. high rotational speed of the whirling units of whirling machine leads to generate High heat during manufacturing process . This condition will affect the accuracy of the manufacturing process of the worm shaft. In this paper the thermal analysis of whirling unit in the whirling machine was conducted in provide order to information for importing the substituted parts.
손동혁(Donghyeok Son),이건(Jian Li),김병국(Byongkuk Kim),문지우(Jiwoo Moon),조윤현(Yunhyun Cho) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.10
This paper presents a dynamic force analysis of switched reluctance motor using 2D-transient finite element method. The main exciting of the vibration is the electiromagnetic force in the stator that is calculated Maxwell stress method. The advantage for elimation of vibration of 12/8 SRM was explained by comparing the radial force of 12/8 and 6/4 SRM in both time and frequency domain. In order to effective mitigate the vibration during turning off process, effects of different commutation method were investigated.
Wang, Lan,Son, Hee-Jeong,Xu, Ming-Lu,Hu, Jian-He,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3
Broussonetia papyrifera radix, fruits, leaves, and stems exhibit antioxidant, antinociceptive, antityrosinase, anti-inflammatory, and antiplatelet activities. However, studies of the anticancer and anti-inflammatory activities of B. papyrifera stem bark are limited. Yet it is important to evaluate these activities to isolate active compounds for potential medicines and food additive. The dichloromethane fraction of B. papyrifera stem bark was found to induce apoptosis-related DNA fragmentation, increase sub-G1 accumulation, increase morphological changes, and inhibit the proliferation of human colon cancer HT-29 cells. Moreover, it significantly increased p53, caspase-3, and Bax expression in HT-29 cells. In addition, the n-butanol fraction inhibited nitric oxide production in RAW 264.7 macrophages by suppressing iNOS expression. These results suggest that B. papyrifera is a potential natural resource for treating cancer and inflammatory diseases.