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- 검색키워드
- 저자 : Ji-Wun Kim (검색결과 254 건)
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( Jeong Sook Kim ),( In Ok Lee ),( Kyung Jin Eoh ),( Young Shin Chung ),( Inha Lee ),( Jung-yun Lee ),( Eun Ji Nam ),( Sunghoon Kim ),( Young Tae Kim ),( Sang Wun Kim ) 대한산부인과학회 2017 Obstetrics & Gynecology Science Vol.60 No.2
Objective This study aimed to introduce a method to remove huge ovarian tumors (≥15 cm) intact with single-port laparoscopic surgery (SPLS) using SW Kim`s technique and to compare the surgical outcomes with those of laparotomy. Methods Medical records were retrospectively reviewed for patients who underwent either SPLS (n=21) with SW Kim`s technique using a specially designed 30×30-cm2-sized 3XL LapBag or laparotomy (n=22) for a huge ovarian tumor from December 2008 to May 2016. Perioperative surgical outcomes were compared. Results In 19/21 (90.5%) patients, SPLS was successfully performed without any tumor spillage or conversion to multi-port laparoscopy or laparotomy. There was no significant difference in patient characteristics, including tumor diameter and total operation time, between both groups. The postoperative hospital stay was significantly shorter for the SPLS group than for the laparotomy group (median, 2 [1 to 5] vs. 4 [3 to 17] days; P<0.001). The number of postoperative general diet build-up days was also significantly shorter for the SPLS group (median, 1 [1 to 4] vs. 3 [2 to 16] days; P<0.001). Immediate post-operative pain score was lower in the SPLS group (median, 2.0 [0 to 8] vs. 4.0 [0 to 8]; P=0.045). Patient-controlled anesthesia was used less in the SPLS group (61.9% vs. 100%). Conclusion SPLS was successful in removing most large ovarian tumors without rupture and showed quicker recovery and less immediate post-operative pain in comparison to laparotomy. SPLS using SW Kim`s technique could be a feasible solution to removing huge ovarian tumors.
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( Jin Hwa Lee ),( Ga Won Yim ),( Eun Ji Nam ),( Sung Hoon Kim ),( Young Tae Kim ),( Sang Wun Kim ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: Demonstrate a new instrument, technique or procedure 방법: In this video, we showed how to remove huge ovarian tumors in sinlgel port laparoscopic surgery by SW KIM`s method. SW KIM`s method is the technique to put a huge ovarian tumor into a specially designed extremely large endopouch (XXXL: 30x30㎠sized) using two conventional laparoscopic needle holders and one laparoscopic grasper and gravity by changing the patient`s position. After identifying the infundibulopelvic and uteroovarian ligaments salpingooophorectomy including huge ovarian tumor was performed by LigaSure. And then ovarian tumor was located in the pelvic cavity by changing the patient`s position into reverse-trendelenberg position and a XXXL endopouch was inserted and un-rolled inside the abdominal cavity. To insert a huge tumor into the XXXL endopouch, the endopouch was opened in triangular shape. Bilateral apex of baseline of trianglular opening of the endopouch was hold by two needle holders and then the upper apex was hold by a grasper. After holding three point of endopouch opeing, the patient`s position was changed into deep trendelenberg postion, then the tumor came into the endopouch by gravity. While changing the patient`s position, 2 needle holders and a graspers were moved into the pelvic cavity from the abdominal cavity. Aftere identifying the tumor inside the endopouch, tumors could be removed through the single port opening site or transvaginally in laparoscopic hysterectomy case without spillage of ovarian tumor. 결과: The advantage of SW KIM`s method is to remove ovarian tumor without spillage in a single port laparoscopic surgery by putting it into the large endoscopic bag despite narrow space. 결론: Using a specially designed 30×30 cm sized XXXL Endopouch and SW Kim`s technique, huge ovarian tumors could be removed without spillage in single port laparoscopic surgery.
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이혜영,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3
Since water borne infection causes acute diseases and results in spread of diseases by secondary infection, the prevention is very important. Therefore, it is necessary to have a method that is rapid and effective to monitor pathogenic bacteria in drinking water. In this study, we employed a systematic method, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, to develop an effective monitoring system for possible bacterial contaminants in drinking water. For this purpose, PCR primers were derived from 992 bp region of the 16s rRNA gene that is highly conserved through the different species of prokaryotes. To test whether the PCR primers designed are indeed useful for detecting all the possible microbial contaminants in the water, the primers were used to amplify 16s rRNA regions of different microbial water-borne pathogens such as E. coli, Salmonella, Yersinia, Listeria, and Staphylococcus. As expected, all of tested microorganisms amplified expected size of PCR products indicating designed PCR primers for 16s rRNA indeed can be useful to amplify all different microbial water-borne pathogens in the water. Furthermore, to test whether these 16s rRNA based PCR primers can detect bacterial populations present in the water, water samples taken from diverse sources, such as river, tap, and sewage, were used for amplification. PCR products were for then subjected for cloning into a T-vector to generate a library containing 16s rRNA sequences from various bacteria. With cloned PCR products, RFLP analysis was done using PCR products digested with restriction enzyme such as Hae Ⅲ to obtain species-specific RFLP profiles. After PCR-RFLP, the bacterial clones which showed the same RFLP profiles were regarded as the same ones, and the clones which showed distinctive RFLP profiles were subsequently subjected for sequence analysis for species identification. By this PCR-RFLP analysis, we were able to reveal diverse populations of bacteria living in water. In brief, in unsterilized natural river water, over 60 different species of bacteria were found. On the other hand, no PCR products were detected in drinking tap-water. The results from this study clearly indicate that the PCR-RFLP-sequence analysis can be a useful method for monitoring diverse, perhaps pathogenic bacteria contaminated in water in a rapid fashion.
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Kim, Hyun-Soo,Kim, Ji-Ye,Lee, Yong Jae,Kim, So Hee,Lee, Jung-Yun,Nam, Eun Ji,Kim, Sunghoon,Kim, Sang Wun,Kim, Young Tae Elsevier 2018 Gynecologic oncology Vol.151 No.3
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>To investigate the prognostic value of the expressions of programmed cell death ligand 1 (PD-L1) and immune checkpoint markers in residual tumors after neoadjuvant chemotherapy (NAC) for advanced high-grade serous ovarian cancer (HGSOC).</P> <P><B>Methods</B></P> <P>We collected pre- and post-NAC tumor samples from patients with advanced HGSOC between 2006 and 2017. Post-NAC tumor tissue samples were available for immunostaining from 131 patients. The expressions of PD-L1 and immune checkpoint markers were assessed by immunohistochemical staining and the status of tumor-infiltrating lymphocytes (TILs) was also evaluated. We examined whether there are significant associations between protein expression status and patient outcomes and whether significant changes in protein expression levels occurred in response to NAC.</P> <P><B>Results</B></P> <P>PD-L1 expression in the tumor cells was evaluated in 113 patients, 12 (10.6%) of whom had high PD-L1 expression (≥25%) in post-NAC tissues. However, these high levels were not associated with progression-free survival (PFS; P = 0.348) or overall survival (OS; P = 0.699). Similarly, high stromal TILs [≥50%; n = 16 (15.0%)] among the 107 patients evaluated did not show any significant impact on PFS (P = 0.250) or OS (P = 0.800). Moreover, an abundance of TILs (intraepithelial, CD8+, and Foxp3+) and the expression of immune checkpoint markers (PD-1, ICOS, and LAG-3) in residual tumors did not confer any significant survival benefit. The impact of NAC on PD-L1 expression and stromal TILs varied considerably among individual patients.</P> <P><B>Conclusion</B></P> <P>Although the expression of PD-L1 and immune checkpoint markers in residual tumors after NAC had no prognostic impact on survival in patients with HGSOC, post-NAC evaluation of these proteins in chemoresistant tumors may help select patients for immunotherapy trials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PD-L1 expression and stromal TILs were low in most residual tumors after NAC. </LI> <LI> High expression of immune parameters did not show any significant impact on survival. </LI> <LI> The impact of NAC on immune parameters varied considerably among individual patients. </LI> <LI> This study highlights the importance of evaluating the post-NAC samples to guide adjuvant treatment. </LI> </UL> </P>
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Long non-coding RNA <i>HOTAIR</i> is associated with human cervical cancer progression
KIM, HEE JUNG,LEE, DAE WOO,YIM, GA WON,NAM, EUN JI,KIM, SUNGHOON,KIM, SANG WUN,KIM, YOUNG TAE D.A. Spandidos 2015 International journal of oncology Vol.46 No.2
<P>The functions of many long non-coding RNAs (lncRNAs) in human cancers remain to be clarified. The lncRNA Hox transcript antisense intergenic RNA (<I>HOTAIR</I>) has been reported to reprogram chromatin organization and promote breast and colorectal cancer metastasis, the involvement of lncRNAs in cervical cancer is just beginning to be studied. In the present study, we examined the expression and the functional role of <I>HOTAIR</I> in cervical cancer. <I>HOTAIR</I> expression was determined in cervical cancer tissues (n=111) and corresponding normal tissues (n=40) by using real-time polymerase chain reaction, and its correlation with clinical parameters and prognosis were analyzed. To determine the effect of <I>HOTAIR</I> knockdown and overexpression in cervical cancer cell lines, we used the CCK-8 assay, wound healing migration and Matrigel invasion assay. The expression level of <I>HOTAIR</I> in cervical cancer tissues was higher than that in corresponding non-cancerous tissues. High <I>HOTAIR</I> expression correlated with lymph node metastasis, and reduced overall survival. A multivariate analysis showed that <I>HOTAIR</I> was a prognostic factor for predicting cervical cancer recurrence. Knockdown of <I>HOTAIR</I> reduced cell proliferation, migration, and invasion in cervical cancer cell lines. Moreover, <I>HOTAIR</I> regulated the expression of vascular endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes, which are important for cell motility and metastasis. Therefore, <I>HOTAIR</I> may promote tumor aggressiveness through the upregulation of VEGF and MMP-9 and EMT-related genes. These findings indicate that <I>HOTAIR</I> may represent a novel biomarker for predicting recurrence and prognosis and serve as a promising therapeutic target in cervical cancer.</P>
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GO-17 : Makorin Ring Finger Protein 1 (MKRN1) as an adjunct marker in liquid-based cervical cytology
( Ji Yun Lee ),( Hyeong Ju Kim ),( Jinkyoung Kong ),( Ji Hee Choi ),( Geum Seon Sohn ),( Hanbyoul Cho ),( Eun Ji Nam ),( Sang Wun Kim ),( Doo Byung Chay ),( Sunghoon Kim ),( Young Tae Kim ),( Jae Hoon 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: The aim of the study was to test the ability of MKRN1 to detect cervical lesion in a screening setting and its use as a surrogate marker of HPV infection. 방법: We conducted PROspective specimen collection and retrospective Blinded Evaluation (PROBE) study. Liquid-based cytology samples were collected from 187 women. A cell block was prepared from residual cervical cytology specimen for immunocytochemistry of MKRN1 and P16 INK4a.Liquid-based cervical cytology, MKRN1, P16 INK4a, P21, P57, KI-67 immunostaining on cell block sections, HPV hybrid capture and real time Polymerase chain reaction (PCR) were performed and analyzed with pathologic results.Clinical performances [sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)] were calculated for all women and subgroup analysis was performed for patients with ASCUS or LSIL. 결과: MKRN1 positivity increased with histologic severity, from 32.4% in CIN1, 60.0% in CIN2, 80.0% in CIN3 to 92.3% in invasive cancer. The sensitivity, specificity, PPV and NPV of MKRN1 to detect CIN2+ was 73.8%, 76.8% 75.6%, and 75.0%, respectively. However, liquid-based cytology showed poorer clinical performance than MKRN1 immunostaining (61.3%, 69.5%, 66.2% and 64.8%, respectively) and HPV testing had lower specificity than MKRN1 (67.7%). When combining two screening tests, MKRN1 + HPV showed the highest sensitivity, specificity, PPV and NPV (71.8%, 85.5%, 82.3%, 76.5%, respectively), while there were no significant differences between cytology + HPV (60.6%, 81.8%, 75.4%, 69.2%) and cytology + MKRN1 (58.8%, 84.1%, 78.3%, 67.7%) in all aspects. In cases of ASCUS/LSIL, MKRN1 + HPV (100%, 72.7%, 73.9%, 100%, respectively) showed the best clinical performances followed by MKRN1 + cervical cytology (100%, 50.0%, 60.7%, 100%, respectively). 결론: MKRN1 was more sensitive and specific than liquid-based cytology, and it was more specific than HPV test. Our study showed that MKRN1 + HPV has the highest sensitivity and specificity when combining two diagnostic tests. As part of screening procedure, MKRN1 could be used as a satisfactory biomarker for the primary screening of cervical cytology.
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Sequence variants of Toll-like receptor 4 (TLR4) and the risk of prostate cancer in Korean men.
Kim, Hae Jong,Bae, Joon Seol,Chang, In Ho,Kim, Kyung Do,Lee, Jaehyouk,Shin, Hyoung Doo,Lee, Ji Youl,Kim, Wun-Jae,Kim, Wonyong,Myung, Soon Chul Springer International 2012 World journal of urology Vol.30 No.2
<P>Chronic inflammation has been considered a potential risk factor for prostate cancer. Toll-like receptors (TLRs) are important in the innate immune response to pathogens and in cross talk between innate immunity and adaptive immunity. In this study, sequence variants in the TLR4 gene were investigated to determine whether they were associated with prostate cancer risk in a Korean cohort.</P>
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Kim, Sang Wun,Kim, Jae Wook,Kim, Young Tae,Kim, Jae Hoon,Kim, Sunghoon,Yoon, Bo Sung,Nam, Eun Ji,Kim, Hye Yeon Wiley Subscription Services, Inc., A Wiley Company 2007 Genes, chromosomes & cancer Vol.46 No.1
<P>The mechanism of drug resistance in cancer is multifactorial, and the accumulation of multiple genetic changes may lead to drug-resistant phenotypes. This study sought to determine characteristic genetic changes in chemoresistant serous ovarian carcinomas using high-resolution array comparative genomic hybridization (aCGH), and identified genomic aberrations that could be used as predictive markers of chemoresistant disease. Seventeen primary ovarian tumors from optimally debulked stage IIIc serous ovarian carcinoma patients were analyzed using aCGH. Ten patients had chemoresistant disease (progression within 12 months of initial chemotherapy), whereas seven patients had chemosensitive disease (no recurrence for more than 36 months). Receiver operating characteristics curve analysis was used to select chromosomal aberrations that could help distinguish chemoresistant disease from chemosensitive disease. In 17 tumors, frequent increases in DNA copy number were seen on 1p36.33, 3q26.2, 8q24.3, 10q26.3, 12p11.21, 20q13.33, and 21q22.3, and frequent losses were observed on 4p12, 5q13.2, 7q11.21, 8p23.1, 14q32.33, Xq13.3, and Xq21.31. The gains on 5p15.33 and 14q11.2, and losses on 4q34.2, 4q35.2, 5q15, 8p21.1, 8p21.2, 11p15.5, 13q14.13, 13q14.2, 13q32.1, 13q34, 16q22.2, 17p11.2, 17p12, and 22q12.3 were more frequent in chemoresistant disease. The losses on 13q32.1 and 8p21.1 had the largest areas under the curve (AUC 0.90 and 0.85, respectively). The most reliable combination of chromosomal aberrations for detecting chemoresistant disease was the loss on 13q32.1 and 8p21.1 (AUC 0.950). Our findings suggest that these chromosomal aberrations are potential predictive markers of chemoresistant disease in patients with serous ovarian carcinomas. © 2006 Wiley-Liss, Inc.</P>
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