Evidence for the Ras-Independent Signaling Pathway Regulating Insulin-Induced DNA Synthesis

Jhun, Byung H. The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.2

The existence of the Ras-independent signal transduction pathway of insulin leading to DNA synthesis was investigated in Rat-1 fibroblasts overexpressing human insulin receptor (HIRc-B) using the single-cell microinjection technique. Microinjection of a dominant-negative mutant Ras protein into quiescent HIRc-B cells inhibited the DNA synthesis stimulated by insulin. Microinjection of oncogenic H-Ras protein (H-Ras) (0.1㎎/ml) induced DNA synthesis by 35%, whereas that of control-injected IgG was induced by 20%. When the maginal amount of oncogenic H-Ras protein was coinjected with a dominant-negative mutant of the H-Ras protein(H-Ras), DNA synthesis was 35% and 74% in the absence and presence of insulin, respectively. This full recovery of DNA synthesis by insulin suggests the existence of the Ras-independent pathway. The same recovery was observed in the cells coinjected with either H-Ras H-Ras plus SH2 domain of the p85 subunit of PI3-kinase(p85) or H-Ras plus H-Ras plus interfering anti-Shc antibody. When coinjected with a dominant-negative Rac1, the DNA synthesis induced by the Ras-independent pathway was blocked. These results indicate that the Ras-independent pathway of insulin leading to DNA synthesis exists, bypassing the p85 of PI3-kinse and Shc protein, and requires Rac1 protein.

Bootstrapping Spatial Median for Location Problems

Jhun, M.,Shin, S. Taylor Francis 2009 Communications in statistics. Simulation and compu Vol.38 No.10

Self-reported Smoking and Urinary Cotinine Levels among Pregnant Women in Korea and Factors Associated with Smoking during Pregnancy

Jhun, Hyung-Joon,Seo, Hong-Gwan,Lee, Do-Hoon,Sung, Moon-Woo,Kang, Yoon-Dan,Syn, Hee Chul,Jun, Jong Kwan The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.5

<P>This study examined urinary cotinine levels and self-reported smoking among pregnant women in Korea and the factors associated with smoking during pregnancy. The subjects were selected from pregnant women who visited 30 randomly sampled obstetric clinics and prenatal care hospitals in Korea in 2006. Smoking status was determined by self-reporting and urinary cotinine measurement. A total of 1,090 self-administered questionnaires and 1,057 urine samples were analyzed. The percentage of smoking revealed by self-reporting was 0.55% (95% confidence interval [CI], 0.11-0.99) and that revealed by urinary cotinine measurement (>100 ng/mL) was 3.03% (95% CI, 1.99-4.06). The kappa coefficient of agreement between self-reported smoking status and urinary cotinine measurement was 0.20 (95% CI, 0.03-0.37). Multiple logistic regression analysis revealed that early gestational period, low educational level, and being married to a smoker were significant risk factors for smoking during pregnancy. Smoking among pregnant women in Korea is not negligible, and those who are concerned to maternal and child health should be aware of this possibility among pregnant women in countries with similar cultural background.</P>

Evidence for the Ras-Independent Signaling Pathway Regulating Insulin-Induced DNA Synthesis

Jhun, Byung H . 생화학분자생물학회 2000 BMB Reports Vol.32 No.2

The existence of the Ras-independent signal transduction pathway of insulin leading to DNA synthesis was investigated in Rat-1 fibroblasts overexpressing human insulin receptor (HIRc-B) using the single-cell microinjection technique. Microinjection of a dominant-negative mutant Ras^(N17) protein into quiescent HIRc-B cells inhibited the DNA synthesis stimulated by insulin. Microinjection of oncogenic HRas^(V12) protein (H-Ras^(V12)) (0.1 ㎎/㎖) induced DNA synthesis by 35%, whereas that of control-injected IgG was induced by 20%. When the marginal amount of oncogenic H-Ras^(V12) protein was coinjected with a dominant-negative mutant of the H-Ras^(V12) protein (H-Ras^(N17)), DNA synthesis was 35% and 74% in the absence and presence of insulin, respectively. This full recovery of DNA synthesis by insulin suggests the existence of the Ras-independent pathway. The same recovery was observed in the cells coinjected with either H-Ras^(V12) plus H-Ras^(V12) plus SH2 domain of the p85 subunit of PI3-kinase (p85^(SH2-N)) or H-Ras^(V12) plus H-Ras^(N17) plus interfering anti-Shc antibody. When coinjected with a dominant-negative Rac1^(N17), the DNA synthesis induced by the Ras-independent pathway was blocked. These results indicate that the Rasindependent pathway of insulin leading to DNA synthesis exists, bypassing the p85 of PI3-kinase and Shc protein, and requires Rac1 protein.

Time Trend and Age-Period-Cohort Effects on Acute Myocardial Infarction Mortality in Korean Adults from 1988 to 2007

Jhun, Hyung-Joon,Kim, Ho,Cho, Sung-Il The Korean Academy of Medical Sciences 2011 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.26 No.5

<P>We examined time trend and age-period-cohort effects on acute myocardial infarction (AMI) mortality in Korean adults from 1988 to 2007. Annual AMI mortality data and population statistics from 1988 to 2007 were obtained from the STATISTICS KOREA website. Age adjusted mortality for four 5-yr calendar periods (1988-1992 to 2003-2007) was calculated by direct standardization using the Year 2000 WHO world standard population. A log-linear Poisson regression model was used to estimate age, period, and cohort effects on AMI mortality. In both genders, age-adjusted AMI mortality increased from period one (1988-1992) to period three (1998-2002) but decreased in period four (2003-2007). An exponential age effect was noted in both genders. The rate ratio of the cohort effect increased up to the 1943 birth cohort and decreased gradually thereafter, and the rate ratio of the period effect increased up to period three (1998-2002) and decreased thereafter. Our results suggest that AMI mortality in Korean adults has decreased since the period 1998-2002 and age, period, and cohort effects have influenced on AMI mortality.</P>

A Study on the Tradition of Tea Ceremony and Cultural Industry in Japan

Jhun Jae Hyun 조선대학교 국제문화연구원 2013 국제문화연구 Vol.6 No.2

his year, as countries short in natural resources, both South Korean and Japanese government and industries have acknowledged that both needs to invest in improving their presence in cultural enterprises to utilize their national powers more efficiently and have been in talks to find a practical method to do so. It should be noted that Dado (다도, 茶道, tea ceremony) is a very important asset as a business and as acentral theme for multiple cultural enterprises. The purpose of this studyisto identify the current state of Japanese Dado (다도, 茶道, tea ceremony) and to utilize that information to develop contents which will boost South Koreas Dado (다도,茶道, tea ceremony). After being organized by Sen no Riky.(千利休 1522~1591) in the 14th century a tea ceremony Meister program was established around JapansE-ye-moto(家元) and became one of the most unique cultural system to represent Japans tea ceremony. Originally tea was brought over to Japan by monks who traveled abroad to Tang Dynasty (618~907) to study Buddhism. They would bring over the tea seeds and plant them in Seburi mountain located in Saga-hyun. Teas popularity hit near rock bottom until in 1911 where Eisai (1141-1215), who built the Shofujuki temple, brought back tea from the Song Dynasty. During the Muromachi period Dado (다도,茶道, tea ceremony) began to establish its basic form by embracing the flashy styles of Higashiyama culture. As a reaction to the overly extravagant ways, Murata Juk. (1432-1510) preferred a more simple and modest approach and established the Wabi tea style based on his philosophy that tea and meditation is one(다선일미, 茶禪一味). His style was passed down to Takeno J..(武野紹., 1502-1555) who in turn passed the torch to Sen no Riky. during the Azuchi-Momoyama period. Sen no Riky. perfected the art of tea ceremony and completed the Wabi tea culture started by Burata Shuko. Wabi tea is considered the original tea ceremony form for Japans tea culture and Sen no Riky. was the one who defined it. Sen no Riky. not only establish and refine the Wabi tea style but also the s.an tea (lit., "grass hermitage") which emphasized on freedom and practicality. Sen no Riky.s pupil Yamanoue Shoji (1544-1590) added his philosophy ofIchigo-Ichieh (一期一會lit., Once in a lifetime) to that of Shikishichisoku of Sen no Riky. and continued to improve upon it. Tea began to reach the general public only after modern era. There are three groups who make up the Sansenke, which is in the very heart of Japanese tea culture, and they are Omote Senke (1613~1672), Ura Senke, Mushano Kouji Senke. Some also consider Yabunouchi Ryu and Sadohayami Ryu and call them the big five. Japan has not only embraced Tea ceremony as part of their cultural heritage but also has turned it into a great medium and utilized it in various business and content creations to raise its marketability. First of all, various venues such as Tanabe museum, Irimashi museum, Tea information center and culture centers hold tea ceremonies and classes where everyone can join in for anominal fee. Second, places such as Numas Koyotei Memorial Park, Uahhonjin park, Porenakakawane Tea center, Shijuokas Tea center, Shimistei Park serves tea to the general public and also hold entertainment events with Tea infused into their programs. Third, various mediums such as Ang Donuts, Nihonjinno shiranai Nihongo (Japanese even Japanese don't know), Hanayori Dango, Aoki Sachikos Chabashirakuri comic in weekly publication Times, and Japanese webtoon the Tears of Tea-Larmes de The by Minamo Kaeroo published in EDEN provides multiple angles for tea to reach the general public and last, sales of tea drinks, instant teas, and tea related character products for adding values to teas. As seen above the reasonwhyDado (다도, 茶道, tea ceremony) has become so accessible to the public was due to the abundant amount of information provided via media and public services and the sales of various tea and tea related products. Enjoying tea is no longer a luxury but apart of every days life as ahobby or for fashion. South Korea should learn and absorb the strong points of Japanese practices and begin spreading the Dado (다도, 茶道, tea ceremony) spirit by naturally augmenting Dado (다도, 茶道, tea ceremony) into everyday activities.

Phenylarsine Oxide Causes an Insulin-dependent, GLUT4-specific Degradation in Rat Adipocytes

Jhun, Byung H.,Hah, Jong S.,Jung, Chan Y. 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-

An incubation of rat adipocytes with phenylarsine oxide(PAO) and then with insulin caused an inhibition of 3-0-methylglucose equilibrium exchange flux and a parallel reduction in cellular GLUT4 content detected by Western blots. Both the transport inhibition and the GLUT4 reduction were saturable with an increasing concentration of PAO showing essentially an identical K. value of 35 ㎛. Both effects were not observed in the absence of insulin or if cells were incubated with insulin first. The reduction was specific to GLUT4; the immunoreactivities of GLUT1, insulin receptor, and clathrin were not affected in these experiments. The GLUT4 reduction occurred only in intact cells and was not observed in homogenized cells or fractionated membranes. GLUT4 reduction was not observed in the presence of chloroquine or at 18℃suggesting involvement of the lysosomal pathway. Based on these results, we propose that there is a PAO-sensitive protein mechanism that controls an insulin-dependent GLUT4 degradation pathway in adipocytes. This protein mechanism and the GLUT4 degradation pathway may play an important role in determining the steady-state GLUT4 level in the insulin-sensitive peripheral tissues in normal and diseased states.

Principles and Practices for Managing NTM-PD

( Jhun Byung Woo ) 대한결핵 및 호흡기학회 2023 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.136 No.0

Clustering Method to Identify Gene Sets with Similar Expression Profiles in Adjacent Chromosomal Regions

Jhun, M.A.,Park, T. SPRINGER-VERLAG 2009 ICHIT 2012 Vol.5517 No.-

Four Domain Vertical Aligned Nematic Mode by Rubbing

Jhun, Chul Gyu,Gwag, Jin Seo Informa UK (TaylorFrancis) 2015 Molecular Crystals and Liquid Crystals Vol.613 No.1