심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

전분 종류를 달리한 무화과편의 품질 특성

김병숙,정미란,이영은 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.6

The effects of various kinds of starch (mungbean, potato, corn, and a blend of potato and corn starch) on the quality characteristics of muhwakwa-pyun were studied according to the storage duration. The sweetness, pH and total acidity of muhwakwa extract were analyzed, as were the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches. For quality characteristics, color difference, instrumental texture characteristics and sensory characteristics were compared. The sweetness of muhwakwa extract was satisfactory but the total acidity was low for Kwa-pyun manufacture. Therefore, its pH was adjusted to 3.4 by adding 10% citric acid with an amount of 1% of total liquid. From the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches, a blend of potato and corn starch was found to be easy to cook and form a gel but a 1ittle more resistant than the other starches in terms of retrogradation. Lightness (L) and yellowness (b) of Kwa-pyun increased while its redness (a) decreased by the storage. The color became whiter and the clarity decrease regardness of the kinds of starch, which became obvious after 3 days of storage. The hardness, springiness, adhesiveness and cohesiveness tended to decrease with storage, which was weak in a blend starch. The overall acceptability of Kwa-pyun made with a blend of potato and corn starch was evaluated as being the best among the samples tested. Therefore, for traditional kwa-pyun manufacture mungbean starch might be replaced with a blend of potato and corn starch which is less expensive and easily available in the market nowadays.

주부의 토종닭에 대한 인식 및 이용실태에 관한 조사

한재숙,한경필,김정숙,김미향 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

This survey was carried out as part of a basic works to improve the extention of native chicken uses. A sample of 282 housewives living in the downtown area of Taegu city were examined using an original questionnaire about their awareness and uses of a native chicken. The results were as follows : The awareness for a chicken dish is significantly different according to the housewives' ages and many respondents evaluated the nutritive value of a native chicken. The housewives preferred chicken boiled plain, spice fried chicken and stewed chicken, in the order. But family members preferred chicken boiled plain, spice fried chicken and french fried chicken, in the order. The frequencies of chicken cooking and buying chicken dishes of the housewives were once per month and they regarded a native chicken as one of traditional, health and natural foods. They appreciate the taste of a native chicken highly and recognized the price of a native chicken expensive. Also 52.5% of housewives could distinguish a native chicken from the others.

조리 방법에 따른 닭의 기계적·관능적 특성 : 토종닭을 중심으로 For the Focus on Native Chicken

한재숙,한경필,김정숙,김미향 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

This study was carried out as part of a basic works to improve the native chicken uses. The mechanical test on the native chicken boiled plain broth and the sensory test was surveyed the preference of three kinds of chickens(native chicken, whangchu, broiler) prepared with six different cooking methods-chicken boiled plain, chicken broiled in microwave, stewed chicken, chicken fried with spice, chicken cutlet, chicken curry·using 120 Yeungnam University students and graduate students as the panel. The results were as follows: The panels preferred the viscosity of chicken boiled plain broth for two hours. The viscosity of chicken boiled plain broth for two hours with the spindle at 5, r.p.m. of 20. 50. 100 was 8.6, 13.6, 9.9 respectively, and salt concentration was 0.40wt%. In the sensory test by cooking methods, the results of preference are in the following order-chicken curry, chicken cutlet, chicken fried with spice, stewed chicken, chicken boiled plain and chicken broiled in microwave. The most preferred cooking method of the native chicken, whangchu and broiler is chicken boiled plain, broiled in microwave and stewed chicken respectively. They preferred the native chicken in texture and taste. But in color, aroma and appearance among the sensory characteristics, whangchu was preferred by the panels.

한국의 외식문화 변천에 관한 연구

尹淑賢,李媄貞 호남대학교 산업기술연구소 2003 산업기술연구논문집 Vol.11 No.-

서울지역 일부 과체중 및 적절체중 학령기 아동의 신체계측, 체지방률, 혈액지표 및 영양소 섭취 상태의 비교 분석 연구

이정숙,이정윤,임현정,조미란,차성호,조여원 대한영양사협회 2003 대한영양사협회 학술지 Vol.9 No.4

This study was carried out to compare the characteristics of the over weight elementary school students with those of desirable-weight children. Anthropometric parameters such as height, weight, BMI body fat percentile, triceps skin fold thickness were measured. Dietary assessment and blood analysis were performed. The average ages of over weight children and desirable-weight children were 10.9f2.5 and 10.2±2.8 years, respectively. The means body fat percentile of over weight children and desirable-weight children were 27.5±3.7% and 20.2± 3.8%. respectively. Plasma levels of TG. total cholesterol, LDL cholesterol, and HDL cholesterol of over weight children and desirable-weight children were 179.3±30.0. 104.9f26.6, 52.3f6.7 and 178.3±84.l㎎/dl and those of desirable-weight children were 86.1±43.5, 183.7f16.5, 101.0f2.1 and 67.7±20.0㎎/dl respectively. Plasma TG levels of over weight children were significantly higher than those of desirable weight chddren. On the other hand plasma HDL-cholesterol levels of over weight children were significantly lower than those of desirable-weight children Plasma levels of GOT ,and GPT were in normal range in both group. However, GPT level of over weight children was &her than that of desirable weight children. Plasma levels of albumin, globulin and BUN were all in normal range and no differences were found in both groups. Hematologic data did not show any difference between two group except WBC, KBC and Hgb levels which were significantly higher in over weight group than those of desirable-weight children. DAY nutrients intake of over weight children and desirable-weight children were very similar and they were around the Korean KDA levels for each nutrients. However, the intake of calcium of both groups were little over 50% of KDA. The consumption of d e b fiber was very low and the cholesterol intake was very high shorn that the habitual meals of Korean school children were not well balanced.

성별에 따른 대학생의 내면화된 수치심과 대인관계 문제가 우울증상에 미치는 영향

최재정,김미연,박민지,유미숙 숙명여자대학교 아동연구소 2015 兒童硏究 Vol.27 No.1

본 연구는 대학생의 내면화된 수치심과 대인관계가 우울증상에 미치는 영향력을 규명하고자 한 연구이다. 연구 대상은 서울, 경기 지역의 대학생 404명을 대상으로 자기보고식 설문조사를 실시하였다. 본 연구에 사용된 측정도구는 대학생의 내면화된 수치심, 대인관계, 우울증상의 척도였다. 분석방법은 SPSS 19.0 통계프로그램을 이용하여 빈도와 백분율을 산출하였고, 내적합치도 계수(Cronbach a)를 구하였다. 본 연구문제를 여자 검증하기 위해 중다회귀분석과 단계적 회귀분석을 실시하였고, 밝혀진 결과를 요약하면 다음과 같다. 첫째, 여자 대학생은 사회적 회피와 헌신성이 우울증상과 관계가 있는 것으로 나타났다. 둘째, 남자 대학생은 실수 불안과 지배성, 비주장성이 우울증상에 영향을 미치는 것으로 나타났다. 셋째, 여학생과 남학생의 결과 차이에서 모두 우울 증상에 유의미한 영향을 미치는 것으로 나타났고, 수치심의 하위요인보다 대인관계문제의 하위요인들이 우울 증상에 보다 많은 영향을 미친다. 수치심보다 대인관계 문제가 우울 증상에 보다 큰 영향을 미친다는 결과는 남녀 대학생 모두에게 공통적으로 나타났다.

크릴분말이 콜레스테롤 및 카드뮴 식이 흰쥐의 간 및 심장 지질에 미치는 영향

조영숙,박석규,박정로,손미예,문주석,곽수동 순천대학교 기초과학연구소 1992 基礎科學硏究誌 Vol.3 No.-

In order to investigate effects of krill on lipid components of liver and heart in cholesterol and cadmium-fed rats, male Sprague-Dawley rats were raised for 4 weeks with 8 experimental diets. Krill used in this experiment contained 65.4%(w/w) of protein and 16.7% of lipid. Krill contained 35% of polyunsaturated fatty acids and more unsaturated fatty acids than saturated fatty acids by 2 times. Amino acid content of krill was high in order of Glu, Asp, Tyr and Lys. Rats fed krill diet showed more food intake and weight gain compared with control group. However, food efficiency ratio and weight of liver and heart were not significantly different. The ingestion of cadmium resulted in a severe restriction in growth rate with normal or mild reduction of liver and heart weight. Cadmium also caused a significant decrease in food efficiency ratio. The contents of total and free cholesterol of liver and heart in rats fed krill diets were similar or slightly higher than those fed control diet. Dietary krill also showed no significant difference in liver and heart cholesterol levels in rats with cadmium ingestion. The contents of phospholipid and triglyceride in liver and heart of krill group were slightly higher than those of control group. Supplement of krill reduced triglyceride content of liver in rats ingested with cadmium without any effect on the level of phospholipid and heart lipids. The concentration of cadmium in plasma significantly increased with dietary krill. However, the krill supplement did not influence the concentration of cadmium in liver or heart. A significant accumulation of lipid in liver tissue was observed in all dietary groups but standard group. However, no difference in degree of lipid accumulation was found among the dietary groups. Necrosis and hemolysis of liver in all dietary groups were not shown.

타우린을 첨가한 김치의 발효 특성

김미숙,정윤화 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5

This study was conducted to investigate the chemical and microbiological properties of taurine added Kimchi during fermentation at 20℃. A control group was salted with 10% brine solution, and a taurine group was salted with 10% brine solution including 5% taurine and was divided into two groups depending on added taurine concentration after brining; 0% taurine (Taurine Ⅰ)and 3% taurine added(Taurine Ⅱ). The pH of Kimchi was markedly decreased over time in all groups and there was no significant difference between groups. Total acidity was the highest in Taurine Ⅱ followed by Taurine Ⅰ and control group during five days of fermentation. The number of total microbe and lactic acid bacteria showed increase similarly in all groups.