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RISS 인기검색어
- 검색키워드
- 저자 : Jeong Hyeonji (검색결과 32 건)
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( Hyeonji Kim ),( Jeong Seong Yang ),( Minji Kang ),( Ahn Nam Joung ),( Cha So Jeong ),( Nakwon Kwak ),( Jae-joon Yim ),( Young Ae Kang ),( Jong-seok Kim ),( Hwa-jung Kim ),( Gyeong In Lee ),( Seung H 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-
Background Nontuberculous mycobacterial pulmonary disease (NTM PD) is increasing not only in South Korea but also worldwide. The seriousness of NTM PD is further raised because of the high relapse rate and low cure rate in its treatment. For the optimization of treatments for NTM PD patients, the first multicenter prospective cohort study named ‘NTM-KOREA’ involving eight hospitals and two institutes in South Korea and East Asia was initiated. One of these institutions, the Korean Tuberculosis Research Institute (KIT), plays the role of a strain bank through the Korea Mycobacterium Resource Center (KMRC) collecting and preserving clinical isolates from NTM-KOREA. Methods In South Korea, all NTM drug susceptibility tests have been performed at KIT except one tertiary hospital. When the patient's treatment is initiated, when the negative conversion fails after treatment, or when relapse/reinfection is observed, a drug susceptibility test is requested, and the clinical isolates are then stored in KMRC. Some strains are specifically registered in a mycobacterial extract library consisting of cell wall lipidomes and secreted proteomes. These strains are cultured on solid medium, and by observing the colonies, it is possible to confirm the presence of mixed infection and the rough or smooth colony surface morphology concerning NTM disease progression. Results From October 2018 to the present, a total of 10,510 NTM isolates of NTM-KOREA have been collected, of which 1,387 strains have been registered in the extract library additionally. Conclusion Despite various studies on NTM PD, the existence of biomarkers and bacterial factors applicable to diagnosis and treatment is still not satisfactory. In the future, genetic mutations will be searched through whole genome sequencing (WGS) analysis of treatment-failing/relapse strains, and related SNP will be searched and selected by comparing them with the control group. We will continue to investigate the characteristics of Korean NTM isolates extensively.
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( Hyeonji Kim ),( Jeong Seong Yang ),( Minji Kang ),( Seung Heon Lee ),( Nakwon Kwak ),( Jae-joon Yim ),( Young Ae Kang ),( Hong Jo Choi ),( Jong-seok Kim ),( Hwa-jung Kim ),( Jake Whang ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0
Background Nontuberculous mycobacteria pulmonary disease (NTM PD) is rapidly increasing worldwide, including in south Korea. As part of the trend to find biomarkers for NTM PD, a multicenter prospective observational cohort study named ‘NTM-KOREA’ involving seven hospitals and two institutes in south Korea was initiated. In order to discover meaningful bacterial factors from preserved strains, the central role of Korean Mycobacterium Resource Center (KMRC) in KIT has been extended from strain preservation to research since 2018. Methods In South Korea, all NTM drug susceptibility tests have been performed at KIT except one tertiary hospital. When the patient's treatment is initiated, when the negative conversion fails after treatment, or when relapse/reinfection is observed, a drug susceptibility test is requested, and the clinical isolates are then stored in KMRC. Some strains are specifically registered in a mycobacterial extract library consisting of cell wall lipidomes and secreted proteomes. These strains are cultured on solid medium, and by observing the colonies, it is possible to confirm the presence of mixed infection and the rough or smooth colony surface morphology concerning NTM disease progression. Results From October 2018 to the present, a total of 7,000 NTM isolates of NTM-KOREA have been collected, of which 350 strains have been registered in the extract library additionally. Through a recent joint study with Seoul National University Hospital, we found that the treatment rate of clofazimine as an NTM treatment is related to minimum inhibitory concentration of each clinical isolate. Conclusions Despite various studies on NTM PD, the existence of biomarkers and bacterial factors applicable to diagnosis and treatment is still not satisfactory. When considering as an infectious disease, the causative agent is also very important as well as biomarkers. Accordingly, we will continue to investigate the characteristics of Korean NTM isolates extensively and to select prominent bacterial factors.
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RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos
Hyeonji Lee,Dong Wook Han,Seonho Yoo,Ohbeom Kwon,Hyeonwoo La,Chanhyeok Park,Heeji Lee,Kiye Kang,Sang Jun Uhm,Hyuk Song,Jeong Tae Do,Youngsok Choi,Kwonho Hong Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.6
Objective: R-loops are DNA:RNA triplex hybrids, and their metabolism is tightly regulated by transcriptional regulation, DNA damage response, and chromatin structure dynamics. R-loop homeostasis is dynamically regulated and closely associated with gene transcription in mouse zygotes. However, the factors responsible for regulating these dynamic changes in the R-loops of fertilized mouse eggs have not yet been investigated. This study examined the functions of candidate factors that interact with R-loops during zygotic gene activation. Methods: In this study, we used publicly available next-generation sequencing datasets, including low-input ribosome profiling analysis and polymerase II chromatin immunoprecipitation-sequencing (ChIP-seq), to identify potential regulators of R-loop dynamics in zygotes. These datasets were downloaded, reanalyzed, and compared with mass spectrometry data to identify candidate factors involved in regulating R-loop dynamics. To validate the functions of these candidate factors, we treated mouse zygotes with chemical inhibitors using in vitro fertilization. Immunofluorescence with an anti-R-loop antibody was then performed to quantify changes in R-loop metabolism. Results: We identified DEAD-box-5 (DDX5) and histone deacetylase-2 (HDAC2) as candidates that potentially regulate R-loop metabolism in oocytes, zygotes and two-cell embryos based on change of their gene translation. Our analysis revealed that the DDX5 inhibition of activity led to decreased R-loop accumulation in pronuclei, indicating its involvement in regulating R-loop dynamics. However, the inhibition of histone deacetylase-2 activity did not significantly affect R-loop levels in pronuclei. Conclusion: These findings suggest that dynamic changes in R-loops during mouse zygote development are likely regulated by RNA helicases, particularly DDX5, in conjunction with transcriptional processes. Our study provides compelling evidence for the involvement of these factors in regulating R-loop dynamics during early embryonic development.
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( Jeong Seong Yang ),( Hyeonji Kim ),( Minji Kang ),( Nakwon Kwak ),( Jae-joon Yim ),( Young Ae Kang ),( Jong-seok Kim ),( Hwa-jung Kim ),( Gyeong In Lee ),( Seung Heon Lee ),( Jake Whang ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-
Background Non-tuberculous mycobacteria (NTM) are ubiquitous pathogens in opportunistic infection. Because the types of NTM existing in the living environment are diverse, it is expected that NTM patients will be exposed to new types of NTM bacteria every day. Therefore, cases of mixed infection in non-tuberculous mycobacterium lung disease are very common, and it has already been known that the statistical significance between mixed infection and low treatment success rate. In this study, we confirmed the possibility of the mixed infection among heterogeneous or subspecies-level through various phenotypes in NTM clinical isolates. Methods Total 1,095 clinical isolates of NTM collected by Korea Mycobacterium Resource Center from March 2020 to June 2021 were used. Identification of the species was performed through Mycobacteria Genoblot Assay by extracting DNA of cultured strains. Confirmation of the morphological diversity was observed by cultured colony shapes inoculating strains through three-phase streaking pattern on 7H10 agar medium. Results A total of 1095 clinical strains were identified, and that Results were as follows: 397 M. avium, 397 M. intracellulare, 121 M. abscessus, 62 M. massiliense, 20 M. fortuitum, 43 rare species, and 55 Mixed species. Excluding mixed species, 122 M. avium, 262 M. intracellulare, 9 M. abscessus, 2 M. massiliense, 7 M. fortuitum and 3 rare species had multiple phenotypic colonies in one clinical isolate, respectively. Conclusion Mixed infection through molecular diagnosis was confirmed in 55 out of 1095, but cases of 405 had phenotypic diversity among clinical isolates confirmed as a single species. It suggests that additional mixed infections not confirmed by molecular diagnosis is may exist at the species-level or subspecies-level, or that a phenotypic transition may occur. In order to observe the correct treatment response and the prognosis for NTM disease patients, it is necessary to confirm such a mixed infection or phenotypic transition.
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