합성펩타이드를 이용한 영양배엽세포-특이 가토 다클론 항혈청의 제작

이희섭,오재민,김정중,문형배,김원신,이황희 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

Within the last few years, a different approach to generating protein-reactive antibodies has been developed that has several advantages over conventional immunization. This involves synthesizing short peptide sequences, coupling them to immunogenic carrier molecules, and immunizing animals with the conjugates. 3βHSD(3β-hydroxy-5-ene steroid dehydrogenase; EC 1.1.1.145) is the enzyme of the plasma membrane of human trophoblast and it's cDNA sequence was identified by Nickon et al(Molecular cloning and expression of human trophoblast antigen FDO161G and its identification as 3β-hydroxy-5-ene steroid dehydrogenase. J Reprod Fert 1991;149;156). For the production of trophoblast-specific antibody, we synthesized three oligopeptides that are epitope sites chosen from cDNA sequence of 3βHSD. Oligopetides were coupled with KLH(keyhole limpet hemocyanin) under 25% glutaraldehyde. The trophoblast-specific rabbit polyclonal antisera was produced by conventional methods. This antisera reacts with a 43kDa protein in human placental lysate by Western blotting analysis and The syncytiotrophoblasts and cytotrophoblasts from villi are stained positively with this antisera by immunohistochemistry. Villous trophoblasts were cultured in methionine-free media for 1 hour and [^(35)S]-Methionine for 24 hours. Media and cell lysate were immunoprecipitated with this antisera and 12% SDS-PAGE was performed. In fluorography, bend was not noted in media and 43kDa band was noted in medis and 43kDa band was noted in lysate. It was concluded that anti-3βHSD antibody produced by synthetic peptide was specific to trophoblasts and 3βHSD was membrane-bound protein of trophoblasts.

천연 제올라이트를 이용한 고농도의 칼슘이온 제거 : 이온교환속도 및 이온교환능 Kinetics and Isotherm

안봉규,오재일,윤석민,임남웅 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

The concentrated calcium removal from industrial wastewater by natural zeolite was examined to reduce inhibitory effects of those ions on the biological treatment in down-stream. It is well known that natural zeolite possesses good cation exchange capacity. Kinetic studies by using a batch-type methods demonstrated that the adopted particle size(1-2, 2-2.8, 2.8-4㎜) of zeolites showed similar exchange trends of calcium ions and showed relatively fast exchange reaction within 1 hour in spite of various zeolite dosages. The influence of the temperature on the ion-exchange capacity (isotherm) was examined. It was found that calcium exchange capacity(4.464㎎-Ca^2+/g-zeolite) at low temperature of 5℃ was double up to 9.225㎎-Ca^2+/g-zeolite at the range of 22-40℃. However, ion exchange efficiencies were similar at each condition of pH 6,7,8,10.

초음파 주파수 및 반응조건 변화에 따른 나프탈렌 분해효율과 OH 라디칼의 발생량 비교

박종성(Jong Sung Park),박소영(So Young Park),오재일(Jei Ll Oh),정상조(Sang Jo Jeong),이민주(Min Ju Lee),허남국(Nam Guk Her) 大韓環境工學會 2009 대한환경공학회지 Vol.31 No.2

나프탈렌은 휘발성이 있는 소수성 물질로 발암유발 가능성이 있고, 수생태계에 심각한 영향을 미친다. 본 연구는 초음파의 주파수 및 반응조건별 나프탈렌 분해효율과 OH 라디칼 변화량을 조사하였다. C-18 역상칼럼을 이용한 LC/FLD (1200 series, Agilent)로 나프탈렌을 분석한 결과 MDL (Method detection limit)은 0.01 ppm이었다. 초음파 조사 동안 휘발된 나프탈렌은 거의 검출되지 않았고(0.05 ppm 이하), 반응조 덮개 개폐별 나프탈렌 분해효율은 거의 차이를 보이지 않았다(1% 이내). 초음파 반응온도가 증가할수록 나프탈렌 제거효율은 감소하는 경향(15℃: 95%→40℃: 85%)을 보였고, pH가 낮을수록 나프탈렌 분해효율이 증가(pH 12: 84%→pH 3: 95.6%)하였다. 나프탈렌 초기농도의 감소에 따라 반응속도는 증가하는 경향을 보여주었다(2.5 ppm: 27.3×10(-3) min(-1), 5 ppm: 22.7×10(-3) min(-1), 10 ppm: 19.0×10(-3) min(-1)). 동일한 초음파 조건(2.5 ppm 나프탈렌, 0.075 W/mL, 20℃, pH 6.8)에서 28 kHz의 분해효율이 132 kHz보다 약 1.46배 높았고(132 kHz: 56%, 28 kHz: 82.7%), 유사 일차반응 속도상수(k1)도 약 2.3배 높게 나타났다(132 kHz: 2.4×10(-3) min(-1), 28 kHz: 5.0×10(-3) min(-1)). 초음파 조사 10분 후 H₂O₂ 농도는 132 kHz가 28 kHz보다 약 7.2배 높았지만(132 kHz: 0.36 ppm, 28 kHz: 0.05 ppm), 조사 90분 후에는 28 kHz가 132 kHz보다 1.1배 높았다(28 kHz: 0.45 ppm, 132 kHz: 0.4 ppm). 2.5 ppm 나프탈렌 용액에 132 kHz와 28 kHz 초음파 조사시 발생된 H₂O₂ 농도는 초순수에 초음파 조사한 결과보다 각각 0.1 ppm과 0.05 ppm씩 낮게 나타났다. 혼형(24 kHz)과 배스형(28 kHz) 초음파의 나프탈렌 분해효율은 각각 87%와 82.7%였고, k1은 22.8×10(-3) min(-1)와 18.7×10(-3) min(-1)로 산출되었다. 다주파 복합형 초음파 시스템(28 kHz 배스형+24 kHz 혼형 초음파)의 나프탈렌 분해효율은 단일주파수 24 kHz(혼형)와 비슷한 제거효율을 보였으나(88%), H₂O₂의 농도는 약 3.5배 높게 조사되었다(28 kHz+24 kHz: 2.37 ppm, 24 kHz: 0.7 ppm). 이와 같은 다주파 복합형 초음파 시스템은 OH 라디칼에 의해 산화가 잘 일어나는 물질의 분해에 매우 효과적으로 적용될 수 있을 것으로 예상된다. Naphthalene is a volatile, hydrophobic, and possibly carcinogenic compound that is known to have a severe detrimental effect to aquatic ecosystem. Our research examined the effects of various operating conditions (temperature, pH, initial concentration, and frequency and type of ultrasound) on the sonochemical degradation of naphthalene and OH radical production. The MDL (Method detection limit) determined by LC/FLD (1200 series, Agilient) using C-18 reversed column is measured up to 0.01 ppm. Naphthalene vapor produced from ultrasound irradiation was detected under 0.05 ppm. Comparison of naphthalene sonodegradion efficiency tested under open and closed reactor cover fell within less than 1% of difference. Increasing the reaction temperature from 15℃ to 40℃ resulted in reduction of naphthalene degradation efficiency (15℃: 95%→40℃: 85%), and altering pH from 12 to 3 increased the effect (pH 12: 84%→pH 3: 95.6%). Pseudo first-order constants (k1) of sonodegradation of naphthalene decreased as initial concentration of naphthalene increased (2.5 ppm: 27.3×10(-3) min(-3)→10 ppm : 19.0×10(-3) min(-3)). Degradation efficiency of 2.5 ppm of naphthalene subjected to 28 kHz of ultrasonic irradiation was found to be 1.46 times as much as when exposed under 132 kHz (132 kHz: 56%, 28 kHz: 82.7%). Additionally, its k1 constant was increased by 2.3 times (132 kHz: 2.4×10(-3) min(-1), 28 kHz: 5.0×10(-3) min(-1)). H₂O₂ concentration measured 10 minutes after the exposure to 132 kHz of ultrasound, when compared with the measurement under frequency of 28 kHz, was 7.2 times as much. The concentration measured after 90 minutes, however, showed the difference of only 10%. (concentration of H₂O₂ under 28 kHz being 1.1 times greater than that under 132 kHz.) The H₂O₂ concentration resulting from 2.5 ppm naphthalene after 90 minutes of sonication at 24 kHz and 132 kHz were lower by 0.05 and 0.1 ppm, respectively, than the concentration measured from the irradiated M.Q. water (no naphthalene added.) Degradation efficiency of horn type (24 kHz) and bath type (28 kHz) ultrasound was found to be 87% and 82.7%, respectively, and k1 was calculated into 22.8×10(-3) min(-1) and 18.7×10(-3) min(-1), respectively. Using the multi- frequency and mixed type of ultrasound system (28 kHz bath type+24 kHz horn type) simultaneously resulted in combined efficiency of 88.1%, while H₂O₂ concentration increased 3.5 times (28 kHz+24 kHz: 2.37 ppm, 24 kHz: 0.7 ppm.) Therefore, the multi-frequency and mixed type of ultrasound system procedure might be most effectively used for removing the substances that are easily oxidized by the OH radical.

Determination of Hydroxyapatite Precipitation Condition from the $Ca-PO_4-H_2O$ System

오영제,Oh, Young-Jei 한국분석과학회 2000 분석과학 Vol.13 No.2

수산화물, 탄산염 및 수산화아파타이트(HAp)의 형성과 해리는 용액의 pH에 따라 달라지며 $Ca-PO_4-H_2O$계로부터 균질하고 미세한 HAp, $Ca_{10-x}(HPO_4)_x(PO_4)_{6-x}(OH)_{2-x}(x=0)$, 세라믹 분말을 제조하는데 매우 중요한 인자가 된다. 각 복합이온의 용해도는 pH와 직선적인 관계에 있으므로 용해도 도형은 수산화물, 탄산염 및 수산화아파타이트(HAp)의 평형상수와 용해도적으로부터 계산된 대수 몰농도로 그릴 수 있다. $Ca-PO_4-H_2O$계로부터 $25^{\circ}C$에서 단일상의 $Ca_{10-x}(HPO_4)_x(PO_4)_{6-x}(OH)_{2-x}(x=0)$ 분말을 제조하기 위한 최적의 pH조건은 이론적인 고찰 결과 $10.5{\pm}0.5$로 결정되었다. 실온에서 제조하여 $80^{\circ}C$에서 건조시킨 HAp분말은 75nm의 입자크기를 갖는 미세한 응집분말 이었으며, $1,000^{\circ}C$에서 하소시킨 HAp분말은 450nm 크기의 거의 균질한 형태의 분말을 얻었다. HAp분말들이 응집되어 있었지만 소결후 미세구조는 양호하여 우수한 기계적 특성을 보유하였다. The formation and dissolution of hydroxides, carbonates and hydroxyapatite (HAp), which depend on the pH of solution, are important factor for the preparation of homogeneous and fine HAp, $Ca_{10-x}(HPO_4)_x(PO_4)_{6-x}(OH)_{2-x}(x=0)$, ceramic powder from the $Ca-PO_4-H_2O$ system. Since the solubility of each complex ion is a linear function of pH, the solubility diagram can be obtained by plotting the logarithmic molar concentrations calculated from the values of the equilibrium constants and solubility products for hydroxides, carbonates, and hydroxyapatite. The optimum pH condition for the formation of single phase $Ca_{10-x}(HPO_4)_x(PO_4)_{6-x}(OH)_{2-x}(x=0)$ powder in $Ca-PO_4-H_2O$ system at $25^{\circ}C$ was estimated as $10.5{\pm}0.5$ through the theoretical consideration. The HAp powder dried at $80^{\circ}C$ showed a fine agglomerated particles with a size of 75 nm. The HAp powder calcined at $1,000^{\circ}C$ consisted of nearly homogeneous particles with a size of 450 nm. Even though the dried HAp particles consisted of agglomeration, mechanical properties were superior due to fine microstructure after sintering.

Immobilization of Streptomyces Phospholipase D on a Dowex Macroporous Resin

Jei Oh Yon,김상달,Ji Seon Lee,Bo Geum Kim,남두현 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1

The immobilization of phospholipase D produced by Streptomyces sp. YU100 was evaluated to see it would be practical for industrial applications. To accomplish this, the purified enzyme, which contained 53 unit/mg of protein, was subjected to immobilization on various matrices. When immobilization supports including calcium alginate gel, polyacrylamide gel, and macroporous resin were evaluated, the highest enzyme retention ratio (> 42%) was observed on a Dowex MSA-2 macroporous resin. This may have occurred as a result of the ability of the hydrophobic domain of phospholipase D to interact with the polystyrene backbone of the resin, as well as the ability of the dimethylethanolamine group of the MSA-2 resin to retain the enzyme by forming hydrogen bonds with the acidic residues of the enzyme. Upon the operation of a reactor packed with enzyme that had been immobilized on a Dowex MSA-2 resin, greater than 80% of the initial enzyme activity was retained for 16 days. During the reaction, phosphatidylcholine became bound to the immobilized resin and interfered with the enzyme reaction, therefore, the resin was washed with ethyl ether every 2 h. A process for recovering excessive L-serine from phos-pholipids using the Dowex MR-3 resin was designed, and the separated L-serine was employed again after replacing the amount that was used.

Ion Beam Assisted Crystallization Behavior of Sol-Gel Derived $PbTiO_3$ Thin Films

Oh, Young-Jei,Oh, Tae-Sung,Jung, Hyung-Jin The Korean Ceramic Society 1996 The Korean journal of ceramics Vol.2 No.1

Ion beam assisted crystallization behavior of sol-gel derived $PbTiO_3$ thin films, deposited on bare silicon(100) substrates by spin-casting method, has been investigated. Ar ion bombardment was directly conducted on the spincoated film surface with or without heating the film from room temperature to $300^{\circ}C$. Ion dose was changed from $5{\times}10^{15}$ to $7.5{\times}10^{16}$ $Ar^-/cm^2$. Formation of (110) oriented perovskite phase was obseerved with ion dose above $5{\times}10^{16}\; Ar^+/cm^2$. Crystallization of $PbTiO_3$ thin film could be enhanced with increasing the Air ion dose, or heating the substrate during ion bombardment. Crystallization of the $PbTiO_3$ films by ion bombardment was related to the local heating effect during ion bombardment.

고농도 질소 탈질 반응에서 칼슘이온 및 이온강도의 영향

오재일(Jei Il Oh),하재인(Jae In Ha),심효영(Hyo Young Shim),윤석민(Suk Min Yoon) 大韓環境工學會 2002 대한환경공학회지 Vol.24 No.3

도로 노면 유출수의 비점오염원 배출 특성 - 중금속 및 병원성 미생물

오재일 ( Jei Il Oh ),박상우 ( Sang Woo Park ),최영화 ( Young Hwa Choi ) 한국물환경학회 ( 구 한국수질보전학회 ) 2005 공동 추계학술발표회 Vol.2005 No.-

P-45 : Determination of Hydroxyapatite Precipitation Condition from the Ca-PO4-H2O System

(Young Jei Oh),(Jang Il Gong) 한국분석과학회 1999 학술대회논문집 Vol.- No.-

상향류 충진상 반응조를 이용한 고농도 질산성 질소처리 시스템의 개념적 설계

오재일(Jei Il Oh),윤석민(Seok Min Yoon),박종문(Jong Moon Park),전희동(Hee Dong Chun) 大韓環境工學會 1999 대한환경공학회지 Vol.21 No.10