Molecular identification of the strains of the domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), which are endemic to Korea, based on single nucleotide polymorphisms in mitochondrial genome sequences

Park Jeong Sun,Kim Min Jee,Kim Seong-Wan,Kim Kee-Young,Kim Seong-Ryul,김익수 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

The domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), has been diversified into various strains over a long period. However, methods to distinguish silkworm strains remain limited partially owing to the genetic similarity caused by the long history of domestication. In this study, we developed molecular identification methods to distinguish three domestic silkworm strains, which are endemic to Korea. By comparing publicly available complete mitochondrial genome (mitogenome) sequences of five endemic strains and 34 stock silk worm strains analyzed in a previous study, we detected 15 single nucleotide polymorphisms (SNPs; SNP1–SNP15), which distinguished the following three endemic strains: Sun7ho (SN7), Sandongsammyeon (SDS), and Sammyeonhonghoeback (SMH). We used two SNPs for each strain to identify the three endemic strains. To distinguish each SN7 and SDS from the remaining four endemic and 34 stock strains, the PCRrestriction fragment length polymorphism method was employed using Acu I and Hpa I restriction enzymes, which recognize SNP1 and SNP8, respectively. Additionally, the tetra-primer amplification refractory mutation system PCR method was used to determine the regions containing SNP3, SNP11, and both SNP14 and SNP15 to distinguish SN7, SDS, and SMH, respectively, from the remaining strains. A validation test with additional in dividuals showed that each target strain was clearly recognized, suggesting that mitogenome SNP-based methods can be used to identify three endemic silkworm strains during culture and breeding.

The diagnosis of coronoid impingement using computed tomography

Baik, Jee-Seon,Huh, Kyung-Hoe,Park, Kwan-Soo,Park, Moo-Soon,Heo, Min-Suk,Lee, Sam-Sun,,Choi, Soon-Chul 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.4

Coronoid impingement can cause limitation of mouth opening. In many cases, it appears to be related to the coronoid hyperplasia. We present a case of mouth opening limitation caused by coronoid impingement on the posterior surface of the zygomatic bone without coronoid hyperplasia. The bony changes in coronoid and zygoma including surface irregularity and discontinuity of the cortex and sclerotic change of inner medullary space were noted on computed tomography (CT) scans in different level of axial planes. Through another CT scans in open mouth position could demonstrate that those bony changes were caused by the contact of both surfaces against each other. In case coronoid impingement is suspected of the many possible causes, the open mouth CT scans will be needed to reveal the direct impingement of coronoid on zygoma even without coronoid hyperplasia.

Comparison of Melanoma Subtypes among Korean Patients by Morphologic Features and Ultraviolet Exposure

( Hong Sun Jang ),( Jee Hung Kim ),( Kyu Hyun Park ),( Jae Seok Lee ),( Jung Min Bae ),( Byung Ho Oh ),( Sun Young Rha ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.4

Background: Genetic alterations have been identified in melanomas according to different levels of sun exposure. Whereas the conventional morphology-based classification provides a clue for tumor growth and prognosis, the new classification by genetic alterations offers a basis for targeted therapy. Objective: The purpose of this study is to demonstrate the biological behavior of melanoma subtypes and compare the two classifications in the Korean population. Methods: A retrospective chart review was performed on patients found to have malignant melanoma in Severance Hospital from 2005 to 2012. Age, sex, location of the tumor, histologic subtype, tumor depth, ulceration, lymph node invasion, visceral organ metastasis, and overall survival were evaluated. Results: Of the 206 cases, the most common type was acral melanoma (n=94, 45.6%), followed by nonchronic sun damage-induced melanoma (n=43, 20.9%), and mucosal melanoma (n=40, 19.4%). Twenty-one patients (10.2%) had the chronic sun-damaged type, whereas eight patients (3.9%) had tumors of unknown primary origin. Lentigo maligna melanoma was newly classified as the chronic sun-damaged type, and acral lentiginous melanoma as the acral type. More than half of the superficial spreading melanomas were newly grouped as nonchronic sun-damaged melanomas, whereas nodular melanoma was rather evenly distributed. Conclusion: The distribution of melanomas was largely similar in both the morphology-based and sun exposure-based classifications, and in both classifications, mucosal melanoma had the worst 5-year survival owing to its tumor thickness and advanced stage at the time of diagnosis. (Ann Dermatol 26(4) 485~490, 2014)

Research Articles : Geographic Genetic Contour of A Leaf Beetle, Chrysolina aurichalcea (Coleoptera: Chysomelidae), on the Basis of Mitochondrial COI Gene and Nuclear ITS2 Sequences

( Joong Won Park ),( Sun Young Park ),( Ah Rha Wang ),( Min Jee Kim ),( Hae Chul Park1 ),( Iksoo Kim ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.23 No.1

The leaf beetle, Chrysolina aurichalcea (Coleoptera: Chysomelidae), is a pest damaging plants of Compositae. In order to understand the genetic diversity and geographic variation we sequenced a portion of mitochondrial COI gene (658 bp) and complete nuclear internal transcribed spacer 2 (ITS2) of the species collected from seven Korean localities. A total of 17 haplotypes (CACOI01~CACOI17), with the maximum sequence divergence of 3.04% (20 bp) were obtained from COI gene sequence, whereas 16 sequence types (ITS2CA01~ITS2CA16), with the maximum sequence divergence of 2.013% (9 bp) were obtained from ITS2, indicating substantially larger sequence divergence in COI gene sequence. Phylogenetically, the COI gene provided two haplotype groups with a high nodal support (≥87%), whereas ITS2 provided only one sequence type group with a high nodal support (≥92%). The result of COI gene sequence may suggest the presence of historical biogeographic barriers that bolstered genetic subdivision in the species. Different grouping pattern between COI gene and ITS2 sequences were interpreted in terms of recent dispersal, reflected in the ITS2 sequence. Finding of unique haplotypes and sequence types only from Beakryeng-Islet population was interpreted as an intact remnant of ancient polymorphism. As more samples are analyzed using further hyper-variable marker, further fruitful inference on the geographic contour of the species might be available.

니코틴과 트리암시놀론 아세토니드를 함유하는 생분해성 마이크로스피어의 제조시 분무건조법과 용매증발법의 비교

박선영,조미현,이종화,김동우,지웅길 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.4

The microspheres have been developed as a new drug delivery system. Although many particulate drug carriers, such as liposome, niosome and emulsion, have been introduced, injectable and biodegradable microspheres appears to be a particularly ideal delivery system because the local anesthesia is not necessary for the insertion of large implants and for the removal of the device after the drug release is finished. Biodegradable microspheres with nicotine and triamcinolone acetonide are prepared and evaluated. As biodegradible polymers, PLA (M.W. 15,000, PLA-0015), PLGA (M.W. 17,000, RG 502) and PLGA (M.W. 8,600, RG 502H) are used. This study attempted to prepare and evaluate the nicotine and triamcinolone acetonide-incorporated microspheres, which were prepared by two methods, solvent-evaporation and spray-drying methods. The microspheres, as a disperse system for injections, were evaluated by particle size, size distribution, entrapment efficiency, and in vitro drug release patterns. The differences of preparation method, partition coefficient, types of polymer, and preparation conditions of microspheres influence the particle size, entrapment efficiency, and in vitro drug release patterns.

조혈모세포로 형질도입에 있어서 Vesicular Stomatitis G Envelope의 효과

박지윤,유은선 梨花女子大學校 醫科大學 醫科學硏究所 2002 EMJ (Ewha medical journal) Vol.25 No.1

목적 : 조혈모세포에서 viral vector를 이용한 안정된 유전자 전달은 유전성 혈액질환의 유전자 치료의 실현에 우선되어야 한다. 그러나 실제로 조혈모세포의 생물학적 특성 등으로 인해 유전자 도입율이 현저히 낮아 임상적인 적용에는 제한이 있다. 저자들은 조혈모세포로 VSV-G 외막을 사용한 HIV vector의 형질도입 효과를 알아보고자 하였다. 방법 : VSV-G를 이용한 HIV-1 vector를 calcium phosphate coprecipitation 방법으로 293T 세포에 형질도입시켜 재조합 농축 바이러스를 얻었다. 분리한 CD34+세포에 재조합 농축 바이러스(10배 농축)를 넣은 다음 4㎍/㎖ polybrene(Sigma)를 첨가하였다. 각 well당 RPMI로 총 500㎕로 맞춘 다음 37℃, 5% CO_2 환경에서 배양하면서 24시간, 48시간 후에 형질도입의 효과를 유세포기와 면역형광 현미경으로 분석하였다. 결과 : HIV 재조합 바이러스가 형질도입된 CD34+ 세포는 YFP의 발현으로 초록색의 형광을 나타내었다. 유세포 분석상 CD34+ 세포로 형질도입율은 배양 24시간 후에는 4.7±2.4%였고, 48시간 후에는 5.7±2.7%였다. VSVG를 사용하지 않은 경우에서는 0.23±0.33%로 저조하였다. 결론 : VSV-G 외막에 의한 HIV vector를 사용하여 CD34+ 세포로 형질도입이 효과적임을 증명하였다. 이런 결과는 조혈모세포를 이용한 유전자 치료의 확대에 기여할 수 있을 것이다. Objectives : Stable gene transfer to human pluripotent hematopoietic stem cells is an attractive strategy for the curative treatment of many genetic hematologic disorders. In clinical tral, the level of gene transfer to this cell population have generally been low. In this study we have evaluated the efficiency of gene transfer to human umbilical cord blood(UCB) CD34+cells using vesicular stomatitis virus glycoprotein G(VSV-G) pseudotyped HIV-1 vector. Method : High titers of replication-defective VSV-G pseudotyped HIV-1 based vector encoding the enhanced yellow fluorescent protein were produced by transient transfection. Human CD34+cells purified from UCB were incubated with pseudotyped HIV supernatants for 24-48 hours. The transduction efficiency were measured by marker gene expression under the microscopy and flow cytometry. Results : Transduction rates into CD34+ were low at 0 and 24 hours, reflecting 4.7±2.4% at 24 hours, they were increased to 5.7±2.7% at 48 hours. Conclusion : We demonstrate efficient transduction of purified human UCB CD34+ by HIV vectors pseudotyped with VSV-G. The results extend the lentiviral vector to clinical gene therapy using human pluripotent hematopoietic stem cells.

국내 Fabry disease 환자의 a-Galatosidase A 유전자 돌연변이 검색

박기범,최지혜,강윤성,김선미,정향민,문영준,이광호 中央大學校 基礎科學硏究所 2001 基礎科學硏究所 論文集 Vol.15 No.-

Fabry disease(FD) is an X-linked recessive lysosomal disorder caused by a deficiency of a-galactosidase A(a-Gal A), localized at Xq22. Besides onset of pain and paresthesias in the extremities, FD was diagnosed by absence of a-Gal A activity. In this experiment the a-Gal A activity of Korean FD patients was spectrometrically analysed using an artificial substate, 4-Mrthylumbellifery1-a-D-galactoside. As expected, no a-Gal A activity was detected in lymphocytes and lymphoblastoid cells from FD patients. To screen the mutation in their a-Gal A genes, we performed single strand conformation polymorphism(SSCP) and PCR-direct sequencing form seven a-Gal A exons. The nonsense mutation was identified both in classically affected hemyzygotes and a heterozygote. They showed the C to T transition at nucleotide number 11,002, resulting in a arginine-to-stop(R342X). This result will be applicable for pre- and neonatal detection of FD and to define the genotype/phenotype correlation.

과학탐구활동의 도구조작 맥락에서 탈중심화된 협력과 공유리더십에 관한 사례 분석

박지영,최취임,이선경 이화여자대학교 교과교육연구소 2020 교과교육학연구 Vol.24 No.2

이 연구에서는 과학탐구활동의 도구조작 맥락에서 개별적 및 집단적 상호작용과 활동 중심성의 탐색을 통해 협력의 특징과 리더십의 변화를 살펴보았다. 연구참여자는 서울 소재 고등학교의 과학 동아리 활동에서 탐구활동을 수행한 4명의 학생들이다. 연구진은 학생들의 활동을 직접 관찰하고 녹화 및 녹음하였으며, 활동 후에 참여 학생과 반구조화된 면담을 실시하였다. 수집된 자료는 모두 전사되었고 다양한 방식으로 분석 및 해석되었다. 시간에 따른 학생들의 도구조작 참여유형 및 상호적 참여유형, 그리고 주도성과 중심성의 변화를 분석하고, 협력과 리더십의 관점에서 해석되었다. 연구 결과, 소집단을 구성한 학생들의 협력은 특정 학생의 주도하에 이루어지기 보다는 활동 과정의 목표와 문제 해결 상황에 따라서 중심이 이동하는 협력의 탈중심화 경향을 나타냈다. 동시에, 학생들은 다양한 참여역할을 하면서 리더의 재배치가 일어나고 공유리더십이 출현되었다. 연구 결과를 토대로 과학탐구활동의 도구조작 맥락에서 나타나는 탈중심화된 협력과 공유리더십의 의미를 재고하였다. In this study, the researchers explored the characteristics of collaboration and the change of leadership through the exploration of individual and collective interactions and the centrality of activities in the context of instruments operation of science inquiry activities. The participants were four students who performed a series of project-based inquiry at a high school science club. The researchers observed the participants’ activities and recorded video and audio contents from the activities. The researchers conducted semi-structured interview with them after each activities. All of the collected data were transcribed and interpreted in various perspectives. The participants’ small group activities were analyzed into participation types, the interaction within group members, proactivity and centrality change of instruments operation and interpreted in views of collaboration and leadership. The results showed students' collaboration type kept changing in a small group. The center of activities kept evolving, being centralized and redistributed, depending on goal in activities' processes and problem solving situations rather than being led by a certain student. Consequently, it was showed the tendency of decentralized collaboration. Also shared leadership were emerged as students playing various participation roles and leadership was redistributed. Based on the study results, meaning of students’ collaborative learning in science classes were reconsidered.

두부에 발생한 신경섬유종증의 증례보고

고지영,김선용,신인숙,박창서,조정신,이장렬,김기덕 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.1

Neurofibromatosis, or Von Recklinghausen's disease is inhereted as an autosomal dominent neurocutaneous systemic disease. It is characterized by multiple Cafe-au-lait spots, generlaized cutaneous neurofibromas. It affects one in 3000 births. We observed the clinical, radiologic and histopathologic findings of 3 cases of neurofibromatosis and obtained following results. 1. All patients had multiple Cafe-au-lait spots and neufibromas. 2. Two patients had radiographic changes of pressive erosion and mesodermal dysplasia. 3. Two patients had plexiform neurofibromas and 1 patient had diffuse neurofibromas. Conclusively, we classified these 3 cases as NF-Ⅰ.

분산형 전원의 계통 연계에 따른 영향 및 시뮬레이션

심재선,박지웅,윤창대,임창민,장 현 三陟大學校 2006 論文集 Vol.39 No.-

As EG(Embedded Generation: photo- voltaic, wind, combined heat and power, fuel cell, small hydro etc.) grows fast in adopting to peak load reducing at the middle or the end of distribution system, there much has been interested in interconnection of EG. This paper discusses the various issue of a embedded generator to power system and shows the simulation of its various situation that could happen(focusing on load-flow by EG) by using a commercial software CYME(PSAF) for load-flow. With a result of above simulation, this paper shows a way of possible solution briefly.