FCL(5-FU, Carboplatin, Leucovorin) 항암 화학요법에서 Gm-CSF의 효과

최지영,김현수,김종숙,박상준,윤환중,조덕연,남상륜,김삼용 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background: One of the major side effects of cancer chemotherapy is myelosuppression. Neutropenia and/or thrombocytopenia are dose-limiting factors in chemotherapy. Colony-stimulating factor induces proliferation and functional maturation of hematopoietic progenitor cells. GM-CSF is primarily active on progenitor cells of granulocytic and monocytic lineage. Methods: Fifteen patients with histologically proven malignancy diagnosed at Chungnam National University Hospital from January 1993 to August 1995 were included in this study. We could evaluate the clinical efficacy of GM-CSF in 13 patients undergoing FCL(5-FU, Carboplatin and Leucovorin) chemotherapy; the first cycles involved no GM-CSF while the second cycles involved GM-CSF on day 6 through 15 of chemotherapy. Results: 1) The subjects were fifteen patients in all, there were five patients with head and neck cancer, which was the most common types of maligancy. There were four patients with colon cancer, two patients with stomach cancer, and one patient with breast cancer, gallbladder cancer, cervix cancer and cholangiocarcinoma respectively, two patients, who did not complete two cycles of chemotherapy were excluded. 2) Age distribution was from 38 years to 78 years with a median age of 57. 3) In FCL chemotherapy cycles with GM-CSF, the duration of neutropenia(<500/μL) was 0.5±0.3 day, while FCL chemotherapy cycles without GM-CSF, it was 2.9±0.7 day(P=0.008). 3) There was no significant difference in platelet count between the two chemotherapy cycles(P=0.133). 4) Febrile duration without GM-CSF was 4.9±2.1 day, but with GM-CSF the duration was 1.3±0.7 day, which was significantly different(P=0.003). The duration of antibiotics use with GM-CSF was 1.7±1.2 day and without GM-CSF was 6.8±3.2 day, also significantly different(P=0.002). But hospital stay between the two cycles were not significantly different(P=0.064). Conclusion: GM-CSF was effective in preventing or restoring bone marrow depression after FCL chemotherapy.

香砂平胃散이 抗癌 및 免疫調節作用에 미치는 影響

하지용,조영호,이지숙 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

In order to investigate annumor and immune response effect by Hyangsapyungwisan after Sarcoma- 180 cells and methotrexate were treared each other. the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration(IC_50), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by 5ug/well, 2.5ug/well, l.25ug/well. and 0.625ug/well of Hyangsapyung-wism concentration inhibited cell viability significantly. IC_5o for cell viability was 11.59ug/well. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(P<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(P<0.05). 8. Production of interleukin-2 was significantly increased(P<0.05). According to the above results, Hyangsapygwisan had prominent antitumor, and enhance both cellular and humoral immunity in mice.

호르텐스극구흡충에서 Alkaline Phosphatase 및 Acid Phosphatase의 특성

강성구,양용석,박주연,임지애,김인식 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY 1999 Journal of biomedical laboratory sciences Vol.5 No.1

호르텐스극구흡충 성충 (adult worm)을 재료로 하여 acid phosphatase (Acp)와 alkaline phosphatase (Alp)의 조직 세포에 대한 분포 및 동위효소의 특성을 규명하기 위하여 Gomori lead nitrate 및 Gomori calcium 방법 그리고 전기영동법 등을 시행하였다. 조직 내의 Acp 및 Alp의 분포는 충체의 소장 (intestine)과 난황선 (vitellaria) 그리고 인두 (pharynx)에서 강한 반응을 나타냈다. 동위효소 유형은 Acp에서 179.5 및 209.4 kDa 두 개의 분획이 분리되었고, Alp은 145.9, 207.5 및 220.8 kDa의 세 개의 분획이 분리되었다. 열에 대한 안정도는 Alp가 90℃에서 12초 경과 후에 완전히 불활성화 되었으며, Alp의 최적 온도 및 pH는 40℃와 pH 9이었고 Acp의 최적 pH는 5였다. 그리고 Acp 및 Alp의 최대활성도 (unit)는 Acp가 71 범위였고, Alp는 189의 범위에 있었다. 이상에서와 같이 호르텐스극구흡충은 phosphatase가 소화관과 난황선에서 주로 분포하고 있었으며 숙주 체내에서 소화관을 중심으로 대사가 활발하게 이루어지고 있음을 알 수 있었다. This study was aimed to investigate the enzyme-histochemical localization and characteristics of alkaline and acid phosphatase extracted from adult of Echinostoma hortense. Using the Gomori calcium stain and the Gomori lead nitrate satin method, we found that the alkaline and acid phosphatases were localized mostly in the intestine, vitellaria and pharynx of Echinostoma hortense. The three isozymes of alkaline phosphatase and two isozymes of acid phosphatase were separated from Echinostoma hortense by electrophoresis. The isozymes of alkaline phosphatase were 145.9, 207.5, 220.8 kDa and the isozymes of acid phosphatase were 175.9 and 209.4 kDa. The activity of alkaline phosphatase was denatured completely after heating at 90℃ for 12 seconds. The optimum pH and temperature for activity of alkaline phosphatase were about pH 9 and 40℃, while the optimum pH for activity of acid phosphatase was about pH 5. The maximum activity of alkaline phosphatase was at 189 unit, but maximum activity of acid phosphatase was at 71 unit. As the result from above, we observed that alkaline and acid phosphatases funtion mainly in the alimentary tract and vitellaria. Echinostoma hortense performs the parasitism in the intestine of host by using proper isozyme of phosphatase.

Human Papillomavirus Testing with Hybrid Capture Ⅱ and DNA Chip

이덕철,--,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepithelial lesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture Ⅱ for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group Ⅰ (nonspecific chronic cervicitis), Group Ⅱ (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group Ⅲ (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture Ⅱ and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples (55.4%), HPV DNAs were detected by Hybrid Capture Ⅱ. In Group Ⅲ, HPV-positive cases were detected in 15 (20.3%) of 74 patients by Hybrid Capture II. 25 patients with ASCUS cytology were histopathologically examined: 9 cases (36%) were Group Ⅱ. In 18 patients with low-grade SIL cytology, 13 cases (72.2%) were Group Ⅱ and 3 cases (16.7%) were Group Ⅲ. 12 cases (92.3%) were Group Ⅲ of 13 patients with high-grade SIL cytology. The sensitivity of each test was 82% in Hybrid Capture Ⅱ and 53.9% in DNA chip test. And the specificity was 74.3%, 85.7% in Hybrid Capture Ⅱ and DNA chip. In conclusion, Hybrid Capture Ⅱ test is more sensitive than DNA chip in detecting women with cervical neoplastic lesions. Especially, in diagnosing of ASCUS, Hybrid Capture Ⅱ test is more sensitive. Therefore, Hybrid Capture Ⅱ test for cancer-associated HPV DNA is a viable option in the management of women with ASCUS.

Antiparasitic Effects of a Herb Extract from Gentiana scabra var buergeri on Trichomonas vaginalis

Yong-Suk Ryang,Jee-Aee Im,Insik Kim,Yoon-Kyung Cho,Ho-Joong Sung,Ju-Youn Park,Duk-Young Min,Ji-Yong Ha 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.2

We studied antitrichomoniasis with the extract of Gentiana scabra var buergeri, which may be effective in treating infectious diseases. The growth inhibition against T. vaginalis became optimal when the extract concentration was 0.7㎎/㎖ and the cells were seeded at a density of 3×10? per well. After incubation for 12, 24, 36, and 48 hrs, respectively, the number of cells were each 5×10?, 1×10?, 1×10?, and none, respectively. Under the electron microscope, the experimental group showed that the nucleus, karyosomes, and chromatin were weaker than those in the control group. After incubating for 3 hrs, the cells were destroyed completely, and only a remnant remained. The hydrogenosomes disappeared almost. The vacuoles and autophagic vacuoles increased. The cells became regressive form.

A study on the Improvement of Surface Characteristics of STD61 Treated by Plasma Paste Boronizing

Jee,Yong-Kwon,Hwang,Yong-Keun,Yoon,Jae-Hong,Son,Myung-Sook,Nam,Gi-Suk 國立 昌原大學校 産業技術硏究所 1996 産技硏論文集 Vol.10 No.-

The effect of plasma on the rate of formation of boride layer, structural Characteristics was investigated through the paste boronizing of STD61, which is used for wear parts often. The processing parameters include the heat treating temperature of 700~900˚C and the time of 1~5 hours at the fixed condition of working pressure and gas mixing ratio of Argon and Hydrogen. It was shown that the rate of formation of the boride layers by the treatment was still higher than those by the thermo-chemical treatment with little structural difference.

Antiparasitic Effects of a Herb Extract from Gentiana scabra var buergeri on Trichomonas vaginalis

Ryang, Yong-Suk,Im, Jee-Aee,Kim, Insik,Cho, Yoon-Kyung,Sung, Ho-Joong,Park, Ju-Youn,MIn, Duk-Young,Ha, Ji-Yong 대한의생명과학회 2001 Journal of biomedical laboratory sciences Vol.7 No.2

We studied antitrichomoniasis with the extract of Gentiana scabra var buergeri, which may be effective in treating infectious diseases. The growth inhibition against T. vaginalis became optimal when the extract concentration was 0.7 mg/ml and the cells were seeded at a density of 3×105 per well. After incubation for 12,24,36, and 48 hrs, respectively, the number of cells were each 5×105, 1×105, 1×105, and none, respectively. Under the electron microscope, the experimental group showed that the nucleus, karyosomes, and chromatin were weaker than those in the control group. After incubating for 3 hrs, the cells were destroyed completely, and only a remnant remained. The hydrogenosomes disappeared almost. The vacuoles and autophagic vacuoles increased. The cells became regressive from.

난자 세포질내 정자 주입술을 이용한 체외수정시술시 누적임신율에 관한 연구

김석현,문신용,김희선,서창석,최영민,이진용,지병철,최성미,김정구,오선경,류범용,배광범,심순섭 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.1

목적 : 난자 세포질내 정자 주입술(ICSI)을 이용한 체외수정시술시 제2일 배아이식술 및 제3일 배아이식술 상호 간의 시술 결과를 비교 분석하고자 하였다. 연구 방법 : 남성인자 불임환자 및 반복적 체외수정 실패 불임환자를 대상으로 ICSI 시술을 이용한 체외수정시술 174주기 중 제2일 배아이식군 134주기와 제3일 배아이식군 40주기의 시술 결과를 후향적으로 비교 분석하였다. 제3일 배아이식군에서는 M3 배양액을 이용하여 배아를 24시간 추가 체외배양하였다. 결과 : 제2일 배아이식군과 제3일 배아이식군 상호 간에 환자의 연령, 기초 혈중 FSH 농도 등 임상적 특성에 있어서 유의한 차이가 없었으나, 제3일 배아이식군에서 과거력상 체외수정시술의 실패 주기 수가 유의하게 많았다(p$lt;0.05). 시술 결과의 비교에 있어서 과배란유도시 hCG 투여일의 혈중 E2 농도, 채취된 난자의 수, 성숙난자의 수, ICSI 시술 후 수정율, 난할율, 이식된 배아의 수 등은 양군 간에 유의한 차이가 없었다. 제3일 배아이식군에서 누적배아지수(CES)가 유의하게 높았다(p$lt;0.05). 배아이식 주기당 임신율, 배아의 착상율, 임신 초기 자연유산율 및 생존아 분만율은 양군 간에 유의한 차이가 없었으나, 생화학적 임신율 및 다태임신율은 제3일 배아이식군에서 유의하게 높았다(p$lt;0.05). 결론 : ICSI 시술을 이용한 체외수정시술시 제3일 배아이식술을 시행받은 불임환자에서는 고식적인 제2일 배아이식술을 시행한 경우에 비하여 배아이식시 상대적으로 높은 누적배아지수로 인하여 다태임신율이 유의하게 증가할 수 있을 것으로 사료되었다. Objective : To compare the clinical outcomes between Day 2 and Day 3 embryo transfer(ET) groups in in vitro fertilization and embryo transfer(IVF-ET) with intracytoplasmic sperm injection(ICSI). Methods : From May, 1997 to December, 1998, 174 cycles of IVF-ET with ICSI were performed and classified into two groups : Day 2 ET group(n=134) and Day 3 ET group (n=40). In Day 3 ET group, embryos fertilized after ICSI were cultured in vitro for further 24 hours in M3 media. Results : There were no significant differences in the age and BMI of patients, basal serum FSH level, protocol of controlled ovarian hyperstimulation(COH), indication of ICSI, and source of sperm for ICSI between two groups. Only the number of the previous failed IVF-ET cycles was significantly higher in Day 3 ET group(p$lt;0.05). Serum E2 level on hCG day, the numbers of oocytes retrieved after COH, oocytes fertilized after ICSI, and embryos transferred, and the rates of fertilization, cleavage, and implantation showed no significant differences. However, cumulative embryo score(CES) was significantly higher in Day 3 ET group(p$lt;0.05). Although there were no significant differences in the rates of pregnancy per ET, spontaneous abortion, and live birth, the rates of biochemical and multiple pregnancy were significantly higher in Day 3 ET group(p$lt;0.05). Conclusions : In IVF-ET with ICSI, the relatively higher CES may contribute to the higher risk of multiple pregnancy in Day 3 ET group, compared with the conventional Day 2 ET group.

재활운동치료가 견관절 손상 야구투수들의 주관적 통증정도와 등속성 외전/내전력에 미치는 효과

지용석(Yong Suk Jee),한종우(Jong Woo Han) 한국사회체육학회 2002 한국사회체육학회지 Vol.18 No.2

The purpose of this study was to investigate the changes of pain degrees and musculoarticulation functions in 8 baseball pitchers with shoulder joint injuries after the 12 week rehabilitative exercise therapy. 1. Before the rehabilitative exercise therapy, the pain degrees felt at the shoulder involved side were 2.87(±0.64), which showed between `severe` and `fair`. But it decreased after the 12 week rehabilitative exercise therapy. According to the pain scale, the 2nd(14.63%), 4th(29.26%), 6th(29.96%), 8th(60.97%), 10th(64.45%) and 12th(65.16%)weeks were decreased. However, only the 8th(60.97%), 10th(64.45%) and 12th(65.16%)weeks were statistically significant decreased and the pain degree in last 12th week was presented `no or mild pain`. 2. Before the rehabilitative exercise therapy, the musculoarticulation functions of the shoulder involved side was very decreased than the uninvolved side. But most of these variables were increased after the rehabilitative exercise therapy. The PT and PTBW of involved side abductor at 60d/sec were significantly increased 31.49%, 28.57% respectively. The PT and PTBW of involved side adductor at 60d/sec were significantly increased 38.65%, 36.14%. The PT and PTBW of involved side abductor at 180d/sec were significantly increased 26.75%, 24.33% respectively. And the acceleration time was significantly decreased 25%. The PT and PTBW of involved side adductor at 180d/sec were significantly increased 37.09%, 34.15% respectively. And the acceleration time was significantly decreased 28.57%. The PTBW of involved side abductor at 300d/sec was significantly increased 55.88%. The PT and PTBW of involved side adductor at 300d/sec were significantly increased 38.95%, 36.33% respectively. And the acceleration time was significantly decreased 41.66%. In conclusion, the rehabilitative exercise therapy performing during 12 weeks in this study was affected positive changes of pain degrees and muscular function in injured baseball pitchers. Therefore we think that the rehabilitative exercise therapy of this study seems to be available method for baseball pitchers with shoulder joint injuries.

아이스하키 공격수와 수비수간의 체성분, 체격, 체력별 수준 및 등속성 회전력에 대한 비교연구

지용석(Yong Suk Jee),김명화(Myung Hwa Kim),유재현(Jae Hyun Yoo),이창영(Chang Young Lee),변재종(Jae Jong Byun),현광석(Kwang Suk Hyun),이창규(Chang Kyu Lee),유광욱(Kwang Wook Yoo) 한국체육교육학회 2002 한국체육교육학회지 Vol.7 No.1

The purpose of this study was to investigate the difference in physique, physical fitness and isokinetic strength of Forward(FW=10) and Defense(DF=9) in Korean professional ice hockey players. The subjects selected for this study were composed of nineteen male professional ice hockey players. They didn`t have any previous medical or physical problems. They were measured physique, body composition, physical fitness and isokinetic knee strength test. All of data were analyzed utilizing independent t-test by SPSS 10.0. The level of statistical significance was set at p<.05. On the basis of the results analyzed in this study, the conclusion was as follow: 1. The variables of body composition were no significant differences between ice hockey FW players and DF players. 2. In physique variables, the height of ice hockey DF players was significant higher than FW players and remainders of physique variables were no significant differences between both groups. 3. The level of physical fitness variables were no significant differences between ice hockey FW players and DF players. 4. In knee isokinetic strength test, the peak torques of flexor and extensor in a left knee joint of ice hockey between DF and FW players were not significant different, but the peak torques of flexor and extensor in a right knee joint of ice hockey DF players were significantly higher than those of FW players. In conclusion, ice hockey players have the difference in physique and isokinetic knee strength between FW and DF players. Therefore, it is very important that the player should be trained up considering their characteristics according to the players` position.