Structural resemblance of the DNAJA-family protein, Tid1, to the DNAJB-family Hsp40

Jinhwa Jang,Sung-Hee Lee,Dong-Hoon Kang,Dae-Won Sim,Kyung-Suk Ryu,Ku-Sung Jo,Jinhyuk Lee,Hyojung Ryu,Eun-Hee Kim,원형식,김지훈 생화학분자생물학회 2022 BMB Reports Vol.55 No.10

The specific pair of heat shock protein 70 (Hsp70) and Hsp40constitutes an essential molecular chaperone system involvedin numerous cellular processes, including the proper folding/refolding and transport of proteins. Hsp40 family members arecharacterized by the presence of a conserved J-domain (JD) thatfunctions as a co-chaperone of Hsp70. Tumorous imaginal disc1 (Tid1) is a tumor suppressor protein belonging to the DNAJA3subfamily of Hsp40 and functions as a co-chaperone of themitochondrial Hsp70, mortalin. In this work, we performed nuclearmagnetic resonance spectroscopy to determine the solutionstructure of JD and its interaction with the glycine/phenylalaninerichregion (GF-motif) of human Tid1. Notably, Tid1-JD, whoseconformation was consistent with that of the DNAJB1 JD, appearedto stably interact with its subsequent GF-motif region. Collectively with our sequence analysis, the present resultsdemonstrate that the functional and regulatory mode of Tid1resembles that of the DNAJB1 subfamily members rather thanDNAJA1 or DNAJA2 subfamily proteins. Therefore, it is suggestedthat an allosteric interaction between mortalin and Tid1is involved in the mitochondrial Hsp70/Hsp40 chaperone system.

Minireview on Recent Antibody-Related NMR Studies

Jinhwa Jang,김지훈 한국자기공명학회 2020 Journal of the Korean Magnetic Resonance Society Vol.24 No.4

In a relatively short period, monoclonal antibodies have made dramatic success as therapeutics for various diseases such as cancers and autoimmune diseases and become an important development items for many pharmaceutical companies. In order to develop antibody drug, it is important to investigate the structural characteristics of both antibody and antigen. NMR studies on antibody are extremely challenging due to big huddles such as a big size of protein and isotope labeling, nevertheless, several studies have been reported in 10 years. Here, we analyzed 95 papers dealing with antibody-related NMR studies reported in recent 10 years. We categorized papers into 3 types: 1) structural characterization of antibody, 2) structural characterization of antigen using antibody, 3) amyloidosis caused by fragment of antibody. This work would shed new light on antibody-related NMR studies.

Predicting the Infectious Disease Spread Using Floating Population Data in Seoul, South Korea

Jinhwa Jang,Insung Ahn 보안공학연구지원센터 2016 International Journal of Bio-Science and Bio-Techn Vol.8 No.6

Emerging of global infectious diseases threat to worldwide induced numerous patients through person to person infection. In previous study, we investigated effects of traveling nationwide using expressway data on the spread of H1N1 influenza virus in Korea during 2009–2010. As a result, influenza epidemic patterns of the 2009 were correlated with some region traffic flow. In this study, we focused on Seoul which region is a highest density of population in Korea. Also using system dynamics-based simulation of the spread of an infectious disease in each of the 25 districts in Seoul was performed. Consequently, the decrease in the number of infected people in the district with a large floating population size was more significant than that with a high population density. This study is meaningful as it visualized the number of infected people on the map, which includes actual geographical information and the changes over time in the number of individuals that belong to S, I, and R classes of population. Also mathematical model based on Korea unique traffic and population movement information could be used. These results can be applying additional population and traffic data in the future, and support making decisions when establishing an effective infectious disease control strategy.

Effect of other polymer on the electrical conductivity of Polycarbonate/Carbon Black composites

Jinhwa Um,Donghak Kim,Jaewoo Jang,Younggon Son 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Nanoparticle-Functionalized Polymer Platform for Controlling Metastatic Cancer Cell Adhesion, Shape, and Motility

Lee, Hyojin,Jang, Yeongseon,Seo, Jinhwa,Nam, Jwa-Min,Char, Kookheon American Chemical Society 2011 ACS NANO Vol.5 No.7

<P>Controlling and understanding the changes in metastatic cancer cell adhesion, shape, and motility are of paramount importance in cancer research, diagnosis, and treatment. Here, we used gold nanoparticles (AuNPs) as nanotopological structures and protein nanocluster forming substrates. Cell adhesion controlling proteins [in this case, fibronection (Fn) and ephrinB3] were modified to AuNPs, and these particles were then modified to the layer-by-layer (LbL) polymer surface that offers a handle for tuning surface charge and mechanical property of a cell-interfacing substrate. We found that metastatic cancer cell adhesion is affected by nanoparticle density on a surface, and ∼140 particles per 400 μm<SUP>2</SUP> (∼1.7 μm spacing between AuNPs) is optimal for effective metastatic cell adhesion. It was also shown that the AuNP surface density and protein nanoclustering on a spherical AuNP are controlling factors for the efficient interfacing and signaling of metastatic cancer cells. Importantly, the existence of nanotopological features (AuNPs in this case) is much more critical in inducing more dramatic changes in metastatic cell adhesion, protrusion, polarity, and motility than the presence of a cell adhesion protein, Fn, on the surface. Moreover, cell focal adhesion and motility-related paxillin clusters were heavily formed in cell lamellipodia and filopodia and high expression of phospho-paxillins were observed when the cells were cultured on either an AuNP or Fn-modified AuNP polymer surface. The ephrin signaling that results in the decreased expression of paxillin was found to be more effective when ephrins were modified to the AuNP surface than when ephrinB3 was directly attached to the polymer film. The overall trend for cell motility change is such that a nanoparticle-modified LbL surface induces higher cell motility and the AuNP modification to the LbL surface results in more pronounced change in cell motility than Fn or ephrin modification to the LbL surface.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2011/ancac3.2011.5.issue-7/nn202103z/production/images/medium/nn-2011-02103z_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn202103z'>ACS Electronic Supporting Info</A></P>

가변곡률압축 기술적용 알루미늄 컨트롤암 개발

장계원(Gyewon Jang),이우식(Woosik Lee),김대업(Daeup Kim),전진화(Jinhwa Jeon),오개희(Kaehee Oh) 한국자동차공학회 2008 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Aluminum extruded profiles have been mostly used only a few automotive parts until now, such as roof rail, sunroof frame and bumper beams. However, Aluminum extruded materials have been being increased in suspension parts, especially, lower control arm. Most of aluminum suspension parts could be obtained by extrusion and stretch bending and forming technology but the cost of production is a bit high due to complex forming process. In this paper, Curvature extrusion technology was applied for cost reduction and higher quality guarantee by removing forming process. It was fabricated curved extruded profile used by curvature extrusion equipment for removing bending process and designed control arm with optimized profile for taking off forming process. For curvature extrusion and excellent quality it was optimized extrusion condition by controlling and combining extrusion speed and traverse moving speed and distance. For check of durability of control arm, it was carried out endurance test and the result was satisfied.

Design and Methods of the Korean National Investigations of 70,000 Suicide Victims Through Police Records (The KNIGHTS Study)

Eun Jin Na,Jinhwa Choi,Dajung Kim,Heeyoun Kwon,Yejin Lee,Gusang Lee,Maurizio Fava,David Mischoulon,Jihoon Jang,Hong Jin Jeon 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.10

Objective The suicide rate in South Korea was the second highest among the Organization for Economic Cooperation and Development countries in 2017. The purpose of this study is to understand the characteristics of people who died by suicide in Korea from 2013– 2017 and to better prevent suicide. Methods This study was performed by the Korea Psychological Autopsy Center (KPAC), an affiliate of the Korea Ministry of Health and Welfare. According to the Korea National Statistical Office, the number of suicide victims nationwide was estimated to reach about 70,000 from 2013 to 2017. Comprehensive suicide records from all 254 police stations in South Korea were evaluated by 32 investigators who completed a 14-day didactic training program. Then, we evaluated the characteristics of suicide victims in association with disease data from the National Health Insurance Database (NHID), which is anonymously linked to personal information of suicide victims. Results Thirty-one of 254 police stations in the Seoul metropolitan area were analyzed by August 10, 2018. Findings showed that the characteristics of suicide victims differed according to the nature of the region. Conclusion Our results suggest that different strategies and methods are needed to prevent suicide by regional groups.

Development of novel vaccine candidates for Mycobacterium tuberculosis involved in Phagosome Maturation Arrest (PMA)

Seijin Park,Jichan Jang,Hwankyu Kang,Seungbin Lim,Sujin Ahn,Jinhwa Lee,Kevin Pethe 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.2

Effect of socioeconomic disparities on the risk of COVID-19 in eight metropolitan cities, the Korea: a community-based study

Hwang Myung-Jae,Park Shin Young,윤태호,Jang Jinhwa,Lee Seon-young,Yoo Myeongsu,Kim Yoo-Yeon,정해관,Kwon Donghyok,김종헌 한국역학회 2022 Epidemiology and Health Vol.44 No.-

OBJECTIVES: Socioeconomic disparities have been reported as the main risk factors contributing to the spread of coronavirus disease 2019 (COVID-19) at the community level. We conducted an epidemiological study on the risk of COVID-19 incidence using area deprivation indices (DIs) depending on the characteristics of the susceptible population. METHODS: The database of the confirmed COVID-19 cases in eight metropolitan cities, the Republic of Korea, from January 20, 2020 to December 31, 2021, was combined with the area DIs and standardized prevalence of diabetes and hypertension from the community health survey. The relative risk (RR) was estimated using a generalized linear model with Poisson distribution by age group. RESULTS: The risk of COVID-19 incidence increased with the increasing age group, especially in patients aged ≥ 75 years. The RR per interquartile range increment of total social deprivation index (total SDI) was 1.54 (95% confidence interval [CI]: 1.34- 1.70) in the COVID-19 incidence. Especially, the risk of COVID-19 incidence in the first wave was about three times higher in the region belonging to the lowest socioeconomic status than in the region with the highest socioeconomic status. The risk was 3.08 (95% CI: 2.42 to 3.78) based on the total SDI and 3.13 (95% CI: 2.53 to 3.83) based on the social deprivation index. CONCLUSIONS: This study provides scientific evidence that socioeconomic inequity is an important risk factor for the spread of COVID-19. This finding suggests that a mid-to-long-term strategy is needed for the susceptible population to reduce the burden of COVID-19 in the community. KEY WORDS: COVID-19, Socioeconomic disparity, Area deprivation index, Community, Korea