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Monitoring Expression of bphC Gene from Ralstonia eutropha H850 Induced by Plant Terpenes in Soil
Kyung-JaJung,Byung-HyukKim,EungbinKim,Jae-SeongSo,Sung-CheolKoh 한국미생물학회 2002 The journal of microbiology Vol.40 No.4
A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxybiphenyl- 1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al., 1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 108 (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.
Aldose reductase inhibitory activity of the methanol extracts from Korean folk plants
Hye Min Kim(김혜민),Saem Han(한샘),Kyung Choi(최경),Jajung Ku(구자정),Kwang-Woo Park(박광우),Eun Ju Cho(조은주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.2
To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants was investigated. Among fifty four Korean folk plants tested, the MeOH extract of Cedrela sinensis showed highest inhibition of AR (IC50 value, 2.52 ㎍/ml). The plant C. sinensis has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.
Screening of aldose reductase inhibitory activities of Korean folk plants in Chungcheong Province
Dong Gu Lee,Ki Ho Lee,Kyung Choi,Jajung Ku,Kwang-Woo Park,Eun Ju Cho,Sanghyun Lee 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.1
To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants in Chungcheong Province was investigated. Among Korean folk plants tested, the MeOH extract of Persicaria longiseta showed highest inhibition of AR (IC<sub>50</sub> value, 5.14 μg/ml). Consequently, P. longiseta has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.
박인준,곽창원,박준범,한자중,Park,Innjoon,Kwak,Changwon,Park,Junboum,Han,Jajung 한국방재학회 2013 한국방재학회논문집 Vol.13 No.5
최근 늘어나고 있는 폐기물량에 따라 폐기물 매립장의 건설이 증가하고 있으며, 매립장 내 폐기물의 격리, 보호 및 환경오염방지를 위하여 토목섬유가 널리 활용되고 있다. 토목섬유는 주변의 흙과 접촉면을 형성하여 폐기물 매립장의 전단 거동에 영향을 미치게 된다. 폐기물 침출수에 포함된 화학적 요소는 이러한 접촉면 거동에 중요한 영향을 미치며, 지진과 같은 동적하중 또한 접촉면 거동에 미치는 화학적 영향을 가속화시킬 수 있다. 따라서 본 연구에서는 화학적 영향을 고려한 접촉면 전단강도 감소효과를 반영하여 수치해석을 수행하였다. 반복 단순전단시험 결과를 바탕으로 수치해석을 수행한 결과, 접촉면 전단강도 감소를 미 고려시 폐기물 매립장의 동적 안정성을 과대평가할 수 있다고 판단된다. The number of the waste landfill site increases continuously in recent years. Various types of geosynthetics have been utilized in the waste landfill site for encapsulating, isolating, protecting, and so on. Geosynthetics compose the interface with soil and affect the shear behaviors of waste landfill system. The chemical aggressors in the leachate also play a critical role in the interface and it is known that the dynamic loading is able to accelerate manifestation of the effects of chemical attack. In this research, numerical analysis was conducted considering shear stress degradation in the geosynthetic-soil interface due to dynamic load with chemical effect. Cyclic simple shear test results was reviewed and applied to the numerical modeling as well. Consequently, the stability of waste landfill site could be overestimated without considering the shear stress degradation of interface.
Screening of aldose reductase inhibitory activities of Korean folk plants in Chungcheong Province
Lee, Dong Gu,Lee, Ki Ho,Choi, Kyung,Ku, Jajung,Park, Kwang-Woo,Cho, Eun Ju,Lee, Sanghyun Institute of Agricultural Science 2013 Korean Journal of Agricultural Science Vol.40 No.1
To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants in Chungcheong Province was investigated. Among Korean folk plants tested, the MeOH extract of Persicaria longiseta showed highest inhibition of AR ($IC_{50}$ value, $5.14{\mu}g/ml$). Consequently, P. longiseta has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.
Inhibition of Aldose Reductase from Rat Lenses by Methanol Extracts from Korean Folk Plants
김혜민,So-Youn Mok,Jeong Min Lee,조은주,최경,Jajung Ku,Kwang-Woo Park,이상현 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
The inhibition of rat lens aldose reductase (AR) in vitro using methanol extracts from Korean folk plants was investigated. Among them, the extracts of Saussurea grandifolia and Rumex crispus showed highest inhibition of AR. IC50 values of the extracts from S. grandifolia and R. crispus were demonstrated 0.07 and 0.05 mg/ml, respectively. Korean folk plants such as S. grandifolia and R. crispus has a possibility of new natural resources for the inhibition of AR.