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Kim, Eun‐,Mi,Kim, Jaehi,Kim, Yun‐,Gon,Lee, Peter,Shin, Dong‐,Sik,Kim, Mira,Hahn, Ji‐,Sook,Lee, Yoon‐,Sik,Kim, Byung‐,Gee John Wiley Sons, Ltd. 2011 Journal of peptide science Vol.17 No.5
<P><B>Abstract</B></P><P>Identification of substrate specificity of kinases is crucial to understand the roles of the kinases in cellular signal transduction pathways. Here, we present an approach applicable for the discovery of substrate specificity of Ser/Thr kinases. The method, which is named as the ‘high‐throughput phosphorylation profiling (HTPP)’ method was developed on the basis of a fully randomized one‐bead one‐compound (OBOC) combinatorial ladder type peptide library and MALDI‐TOF MS. The OBOC ladder peptide library was constructed by the ‘split and pool’ method on a HiCore resin. The peptide library sequence was Ac‐Ala‐X‐X‐X‐Ser‐X‐X‐Ala‐BEBE‐PLL resin. The substrate specificity of murine PKA (cAMP‐dependent protein kinase A) and yeast Yak1 kinase was identified using this method. On the basis of the result, we identified Ifh1, which is a co‐activator for the transcription of ribosomal protein genes, as a novel substrate of Yak1 kinase. The putative Yak1‐dependent phosphorylation site of Ifh1 was verified by <I>in vitro</I> kinase assay. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.</P>
Ahla Jo,Tae-Han Kim,Dong-Min Kim,김형모,Bomi Seong,Jaehi Kim,Xuan-Hung Pham,정흥수,Sang Hun Lee,Do Won Hwang,Dae Hong Jeong,Yoon-Sik Lee,Dong-Eun Kim,Bong-Hyun Jun 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.90 No.-
As virus spread can lead to severe epidemics and pandemics associated with high mortality, it is necessary to have a highly sensitive detection method for viruses. Although various detection methods have been developed so far, current methods in detecting a virus require preprocessing and involve quite intricate processes of low sensitivity. Here, we have developed a virus detection method with a broad dynamic range and high sensitivity, based on immuno-complex formation between quantum dot (QD)- embedded silica nanoparticles (QD2 ) and magnetic beads. The multiple QD- containing QD2 s showed 500 times stronger photoluminescence than individual QDs. When biotin was immobilized as a ligand, streptavidin was detected in a range of 10 zM to 10 nM. The clinical applicability of the QD2 -based system was examined using the avian virus (i.e., H1N1 influenza virus), and it showed a detection range of 4.76 × 10-4 < span class="xps_thinspace"> ~ 3.2 hemagglutination unit/mL. This result is comparable to the polymerase chain reaction method, and is approximately 2100 times more sensitive than the conventional hemagglutination method. Since the QD2 -based system could detect target molecules with high sensitivity without requiring an amplification step, it can be applied in various biomedical and clinical fields.
Automated Maskless Photolithography System for Peptide Microarray Synthesis on a Chip
Shin, Dong-Sik,Lee, Kook-Nyung,Yoo, Byung-Wook,Kim, Jaehi,Kim, Mira,Kim, Yong-Kweon,Lee, Yoon-Sik American Chemical Society 2010 Journal of combinatorial chemistry Vol.12 No.4
<P>Maskless photolithographic peptide synthesis was performed on a glass chip using an automated peptide array synthesizer system. The peptide array synthesizer was built in a closed box, which contained optical and fluidic systems. The conditions for peptide synthesis were fully controlled by a computer program. For the peptide synthesis on a glass chip, 20 NVOC-protected amino acids were synthesized. The coupling efficiencies of two model peptide sequences were examined on ACA/APTS and PEG/CHI/GPTS chips. PEG/CHI/GPTS chip gave higher average stepwise yields of GIYWHHY (94%) and YIYGSFK (98%) than those of ACA/APTS chip. To quantify peptide−protein binding affinity, HPQ- or HPM-containing pentapeptides were synthesized on a PEG/CHI/GPTS chip and the binding event of Cy3 labeled-streptavidin was quantified. The peptide sequence of IQHPQ showed highest binding affinity with Cy3 labeled-streptavidin. The results demonstrated that the photolithographic peptide array synthesis method efficiently quantified the binding activities of protein-peptide interactions and it can be used for additional biological assay applications.</P><P>A maskless photolithography system for peptide array synthesis on a chip is presented.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jcchff/2010/jcchff.2010.12.issue-4/cc100009g/production/images/medium/cc-2010-00009g_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cc100009g'>ACS Electronic Supporting Info</A></P>
Kang, Homan,Yim, Joonhyuk,Jeong, Sinyoung,Yang, Jin-Kyoung,Kyeong, San,Jeon, Su-Ji,Kim, Jaehi,Eom, Khee Dong,Lee, Hyunmi,Kim, Hye-In,Jeong, Dae Hong,Kim, Jong-Ho,Lee, Yoon-Sik American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.24
<P>To impart a desired optical property to metal nanoparticles (NPs) suitable for surface-enhanced Raman scattering (SERS) applications, it is crucial to assemble them in two or three dimensions in addition to controlling their size and shape. Herein, we report a new strategy for the synthesis and direct assembly of Ag NPs on silica nanospheres (AgNPs-SiNS) in the presence of poly(ethylene glycol) (PEG) derivatives such as PEG-OH, bis(amino)-PEGs (DA-PEGs), and <I>O</I>,<I>O</I>′-bis(2-aminopropyl)PEG (DAP-PEG). They exhibited different effects on the formation of Ag NPs with variable sizes (10–40 nm) and density on the silica surface. As the molecular weight (MW) of DA-PEGs increased, the number of Ag NPs on the silica surface increased. In addition, DAP-PEG (MW of 2000), which has a 2-aminopropyl moiety at both ends, promoted the most effective formation and assembly of uniform-sized Ag NPs on a silica surface, as compared to the other PEG derivatives with the same molecular weight. Finally, we demonstrated that AgNPs-SiNS bearing 4-fluorobenzenethiol on its surface induced the strong SERS signal at the single-particle level, indicating that each hybrid particle has internal hot spots. This shows the potential of AgNPs-SiNS for SERS-based sensitive detection of target molecules.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-24/am404435d/production/images/medium/am-2013-04435d_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am404435d'>ACS Electronic Supporting Info</A></P>
Kang, Taegyu,Jeong, Sinyoung,Kang, Homan,Kim, Jaehi,Kim, Hyung-Mo,Kyeong, San,Lee, Sang Hun,Jeong, Dae Hong,Jun, Bong-Hyun,Lee, Yoon-Sik Elsevier 2015 Journal of industrial and engineering chemistry Vol.32 No.-
<P><B>Abstract</B></P> <P>Surface-enhanced Raman scattering (SERS) has received much attention due to ultra-high sensitivity in detection of biological and chemical analytes. When SERS is used as tagging probe, several strong points such as high sensitivity and multiplexing capability can resolved several key issues with current optical tagging materials. Our group has reported synthesis of silica-coated silver nanoparticles (NPs) embedded silica NPs (SERS Dots) for bio-applications. Here, we designed and synthesized a new type of hollow-shell type SERS active NP (yolk–shell SERS Dots), which are aggregated silver NPs with a hollow silica nanostructure. The yolk–shell SERS Dots were prepared by adding NaBH<SUB>4</SUB> to SERS Dots, resulting in the prepared NPs exhibiting 2.3 times stronger SERS signals due to aggregation of silver NPs inside the silica shells and generating several hot spots among those silver NPs. Moreover, biotin-conjugated yolk–shell type SERS dots successfully bound streptavidin-Cy5 to demonstrate the potential of yolk–shell SERS dots for bio-detection. These yolk–shell SERS Dots have great potential for bio-imaging and drug delivery applications.</P>
Facile Method for Preparation of Silica Coated Monodisperse Superparamagnetic Microspheres
Pham, Xuan-Hung,Kyeong, San,Jang, Jaein,Kim, Hyung-Mo,Kim, Jaehi,Jung, Seunho,Lee, Yoon-Sik,Jun, Bong-Hyun,Chung, Woo-Jae Hindawi Limited 2016 Journal of nanomaterials Vol.2016 No.-
<P>This paper presents a facile method for preparation of silica coated monodisperse superparamagnetic microsphere. Herein, monodisperse porous polystyrene-divinylbenzene microbeads were prepared by seeded emulsion polymerization and subsequently sulfonated with acetic acid/H2SO4. The as-prepared sulfonated macroporous beads were magnetized in presence of Fe<SUP>2+</SUP>/Fe<SUP>3+</SUP>under alkaline condition and were subjected to silica coating by sol-gel process, providing water compatibility, easily modifiable surface form, and chemical stability. FE-SEM, TEM, FT-IR, and TGA were employed to characterize the silica coated monodisperse magnetic beads (~7.5 <I>μ</I>m). The proposed monodisperse magnetic beads can be used as mobile solid phase particles candidate for protein and DNA separation.</P>