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Lee, Jeongyeo,Kim, Hyun-Bi,Noh, Young-Hee,Min, Sung Ran,Lee, Haeng-Soon,Jung, Jaeeun,Park, Kun-Hyang,Kim, Dae-Soo,Nam, Myeong Hyeon,Kim, Tae Il,Kim, Sun-Ju,Kim, HyeRan The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.2
Strawberry (Fragaria ${\times}$ ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was $13.6^{\circ}Brix$ (Okmae, Geumsil, Aram, and Maehyang) and $2.9^{\circ}Brix$ (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from $3.247{\pm}0.056$ to $3.850{\pm}0.055g/100g$, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria ${\times}$ ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.
Lee, Jeongyeo,Jung, Jaeeun,Son, Seung-Hyun,Kim, Hyun-Bi,Noh, Young-Hee,Min, Sung Ran,Park, Kun-Hyang,Kim, Dae-Soo,Park, Sang Un,Lee, Haeng-Soon,Kim, Cha Young,Kim, Hyun-Soon,Lee, Hyeong-Kyu,Kim, HyeRa Hindawi 2018 The Scientific World Journal Vol.2018 No.-
<P>Sophorae Radix (<I>Sophora flavescens </I>Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and<I> p</I>-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.</P>
Jaeeun Yoo,Gun Dong Lee,Jee Hae Kim,Seung Nam Lee,Hyojin Chae,Eunhee Han,Yonggoo Kim,김명신 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.2
Background: Hereditary breast and ovarian cancer syndrome (HBOC) is caused by pathogenic variants in BRCA and other cancer-related genes. We analyzed variants in BRCA gene and other cancer-related genes in HBOC patients to evaluate the clinical validity of next-generation sequencing (NGS) multi-gene panel testing. Methods: The BRCA1/2 NGS testing was conducted for 262 HBOC patients. Multiplex ligation-dependent probe amplification and direct Sanger sequencing were performed for confirmation. Multi-gene panel testing was conducted for 120 patients who did not possess BRCA1/2 pathogenic variants but met the National Comprehensive Cancer Network criteria. Results: Pathogenic variants in BRCA1/2 were detected in 30 HBOC patients (11.5%). Additionally, four out of the 120 patients possessed pathogenic variants by multi-gene panel testing (3.3%): MSH2 (c.256G>T, p.Glu86*), PMS2 (c.1687C>T, p.Arg563*), CHEK2 (c.546C>A, p.Tyr182*), and PALB2 (c.3351-1G>C). All the four patients had a family history of cancer. Conclusions: Multi-gene panel testing could be a significant screening tool for HBOC patients, especially for those with a family history of cancer.
Kim, Minseo,Jang, Jaeeun,Kim, Hyunki,Lee, Jihee,Lee, Jaehyuck,Lee, Jiwon,Lee, Kyoung-Rog,Kim, Kwantae,Lee, Yongsu,Lee, Kyuho Jason,Yoo, Hoi-Jun IEEE 2017 IEEE journal of solid-state circuits Vol.52 No.11
<P>A wearable electrical impedance tomography (EIT) system is proposed for the portable real-time 3-D lung ventilation monitoring. It consists of two types of SoCs, active electrode (AE)-SoC and Hub-SoC, mounted on wearable belts. The 48-channel AE-SoCs are integrated on flexible printed circuit board belt, and Hub-SoC is integrated in the hub module which performs data gathering and wireless communication between an external imaging device. To get high accuracy under the variation of conductivity, the dual-mode current stimulator provides the optimal frequency for time difference-EIT and frequency difference-EIT with simultaneous 4 k-128 kHz impedance sensing. A wide dynamic range instruments amplifier is proposed to provide 94 dB of wide dynamic range impedance sensing. In addition, the 48-channel AE system with the dedicated communication and calibration is implemented to achieve 1.4-m Omega sensitivity of impedance difference in the in vivo environment. The AE-/Hub-SoCs occupy 3.2 and 1.3 mm2in 65-nm CMOS technology and consume 124 mu W and 1.1 mW with 1.2 V supply, respectively. As a result, EIT images are reconstructed with 90% of accuracy, and up to 10 frames/s real-time 3-D lung images are successfully displayed.</P>
Bacterial Sialic Acid Catabolism at the Host–Microbe Interface
Kim Jaeeun,Kim Byoung Sik 한국미생물학회 2023 The journal of microbiology Vol.61 No.4
Sialic acids consist of nine-carbon keto sugars that are commonly found at the terminal end of mucins. This positional feature of sialic acids contributes to host cell interactions but is also exploited by some pathogenic bacteria in evasion of host immune system. Moreover, many commensals and pathogens use sialic acids as an alternative energy source to survive within the mucus-covered host environments, such as the intestine, vagina, and oral cavity. Among the various biological events mediated by sialic acids, this review will focus on the processes necessary for the catabolic utilization of sialic acid in bacteria. First of all, transportation of sialic acid should be preceded before its catabolism. There are four types of transporters that are used for sialic acid uptake; the major facilitator superfamily (MFS), the tripartite ATP-independent periplasmic C4-dicarboxilate (TRAP) multicomponent transport system, the ATP binding cassette (ABC) transporter, and the sodium solute symporter (SSS). After being moved by these transporters, sialic acid is degraded into an intermediate of glycolysis through the well-conserved catabolic pathway. The genes encoding the catabolic enzymes and transporters are clustered into an operon(s), and their expression is tightly controlled by specific transcriptional regulators. In addition to these mechanisms, we will cover some researches about sialic acid utilization by oral pathogens.
Mild Copper–TBAF-Catalyzed <i>N</i>-Arylation of Sulfoximines with Aryl Siloxanes
Kim, Jaeeun,Ok, Jinpyo,Kim, Sanghyuck,Choi, Wonseok,Lee, Phil Ho American Chemical Society 2014 ORGANIC LETTERS Vol.16 No.17
<P>An efficient copper–TBAF-catalyzed C–N bond formation of sulfoximines with arylsiloxanes in dichloromethane at room temperature, affording the desired <I>N</I>-aryl sulfoximines in good to excellent yields under an oxygen atmosphere, is reported. This method complements the existing synthetic approaches due to some advantageous properties of arylsiloxanes such as availability, low toxicity, ease of handling, high stability, and environmental benignity under mild reaction conditions, thus opening a new approach to practical C–N bond formation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2014/orlef7.2014.16.issue-17/ol502174n/production/images/medium/ol-2014-02174n_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol502174n'>ACS Electronic Supporting Info</A></P>
Kim Jaeeun,Kim Byoung Sik 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.9
16S rRNA short amplicon sequencing-based microbiota profiling has been thought of and suggested as a feasible method to assess food safety. However, even if a comprehensive microbial information can be obtained by microbiota profiling, it would not be necessarily sufficient for all circumstances. To prove this, the feasibility of the most widely used V3-V4 amplicon sequencing method for food safety assessment was examined here. We designed a pathogen (Vibrio parahaemolyticus) contamination and/or V. parahaemolyticus-specific phage treatment model of raw oysters under improper storage temperature and monitored their microbial structure changes. The samples stored at refrigerator temperature (negative control, NC) and those that were stored at room temperature without any treatment (no treatment, NT) were included as control groups. The profiling results revealed that no statistical difference exists between the NT group and the pathogen spiked- and/or phage treatedgroups even when the bacterial composition was compared at the possible lowest-rank taxa, family/ genus level. In the beta-diversity analysis, all the samples except the NC group formed one distinct cluster. Notably, the samples with pathogen and/or phage addition did not form each cluster even though the enumerated number of V. parahaemolyticus in those samples were extremely different. These discrepant results indicate that the feasibility of 16S rRNA short amplicon sequencing should not be overgeneralized in microbiological safety assessment of food samples, such as raw oyster.