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      • SCIESCOPUSKCI등재

        Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

        ( Jae-sung Ryu ),( Kyu-tae Chang ),( Ju-taek Lee ),( Malg-um Lim ),( Hyun-ki Min ),( Yoon-ju Na ),( Su-bin Lee ),( Gislain Moussavou ),( Sun-uk Kim ),( Ji-su Kim ),( Kinarm Ko ),( Kisung Ko ),( Kyung- 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.6

        The ACKNOWLEDGEMENTS should be corrected as follows, "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea. The published article in this journal was a part of the 2013 Doctoral thesis of Jae-Sung Ryu submitted to Wonkwang University, Korea." and not "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea."

      • KCI등재

        Evaluating Corrosion Resistance of Additive-Manufactured Ti–6Al–4V Using Electrochemical Critical Localized Corrosion Temperature

        Jae‑Bong Lee,Dong‑il Seo,Hyun Young Chang 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.1

        A new concept to evaluate the localized corrosion resistance of stacked Ti alloys such as additive-manufactured (AM)Ti–6Al–4V alloy is proposed. Crevice corrosion of subtractive-manufactured (SM) Ti–6Al–4V occurred on the surfacebeneath the crevice former, whereas localized corrosion of the AM alloy occurred in any vulnerable site irrespective of thecrevice assembly. The electrochemical critical localized corrosion temperature (E-CLCT) was measured to evaluate theresistance of AM Ti–6Al–4V alloys to localized corrosion. The results showed that the localized corrosion of AM Ti–6Al–4V was attributable to the anisotropy and microstructure that resulted from rapid cooling, which were completely differentfrom the anisotropy and microstructure of SM Ti–6Al–4V. The optimum applied potential of AM Ti–6Al–4V in 25 wt%NaCl aqueous solution was deduced. The E-CLCT provides a useful criterion for determining the resistance of AM Ti alloysto localized corrosion and for comparing their resistances.

      • KCI등재

        Effects of Pilger Rolling and Heat Treatment on Microstructure and Corrosion Resistance of Ag-electroplated 304 Stainless Steel Tubes

        Hyun Park,Woo‑Jin Lee,Jae‑Han Son,Han‑Kyun Shin,Sung‑Kyu Hong,Hyo‑Jong Lee 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

        We introduce a cost-effective method that combines electroplating with metal cold working processes to manufacture compositetubes with robust corrosion resistance. First, a 3-step electroplating process was developed to form an adhesive anduniform Ag coating on the outer wall of a 304 stainless steel tube. The process consisted of a Ni-strike step for removing thepassivation layer of the initial 304 tube, Cu deposition for smoothing the surface by adding a buffer layer, and Ag deposition. To reinforce the interfacial adhesion between the electroplated layers and the 304 tube and increase the area of the Ag coating,a pilger rolling or pilger rolling + heat-treatment process was performed after the Ag electroplating process. Scanningelectron microscopy of the composite tubes after each process indicated that the as-deposited Ag coating changed fromhaving a rough to smooth surface after only pilger rolling and with additional heat treatment. Electron backscatter diffractionanalysis of the microstructures and textures of the tubes revealed that dynamic recrystallization occurred extensively duringpilger rolling, resulting in the formation of a relatively defect-free grain structure without heat treatment. Furthermore,electrochemical polarization curves determined that the Ag-coated composite tubes are superior to the uncoated 304 tubein terms of corrosion resistance under Cl−atmosphere, owing to the formation of a AgCl passivation layer during testing. Surface analysis of the composite tubes suggests that the properties of the AgCl passivation layer are related to the grainsize of the Ag coating and the density of plastic-deformation-induced defects.

      • KCI등재

        Changes in αB-crystallin, tubulin, and MHC isoforms by hindlimb unloading show different expression patterns in various hindlimb muscles

        ( Hyun Seok Jee ),( Taka Shi Skurai ),( Jae Young Lim ),( Hideo Hatta ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.2

        Hyun Seok Jee, Takashi Sakurai, Jae-Young Lim and Hideo Hatta. Changes in αB-crystallin, tubulin, and MHC isoforms by hindlimbunloading show different expression patterns in various hindlimb muscles. JENB., Vol. 18, No. 2, pp.161-168, 2014 [Purpose]αB-crystallin is a small heat shock protein that acts as a molecular chaperone under various stress conditions. Microtubules, whichconsist of tubulin, are related to maintain the intracellular organelles and cellular morphology. These two proteins have been shownto be related to the properties of different types of myofibers based on their contractile properties. The response of these proteinsduring muscular atrophy, which induces a myofibril component change, is not clearly understood. [Methods] We performed 15days of hindlimb unloading on rats to investigate the transitions of these proteins by analyzing their absolute quantities. Proteincontents were analyzed in the soleus, plantaris, and gastrocnemius muscles of the unloading and control groups (N = 6). [Results]All three muscles were significantly atrophied by hindlimb unloading (P < 0.01): soleus (47.5%), plantaris (16.3%), and gastrocnemius(21.3%) compared to each control group. αB-crystallin was significantly reduced in all three examined unloaded hindlimb musclescompared to controls (P < 0.01) during the transition of the myosin heavy chain to fast twitch muscles. α-Tubulin responded onlyin the unloaded soleus muscle. Muscle atrophy induced the reduction of αB-crystallin and α-tubulin expressions in plantar flexormuscles with a shift to the fast muscle fiber compared to the control. [Conclusion] The novel finding of this study is that bothproteins, αB-crystallin and α-tubulin, were downregulated in slow muscles (P < 0.01); However, α-tubulin was not significantly reduced compared to the control in fast muscles (P < 0.01). [Keyword] Microtubule, αB-crystallin, tubulin, myosin heavy chainisoform, skeletal muscle

      • 감속기 기어의 결함진단에 관한 연구

        이재현,국재창,이재응 중앙대학교 기술과학연구소 1999 기술과학연구소 논문집 Vol.29 No.-

        본 논문에서는 감속기 기어의 결함진단의 문제가 고려되었고, 시간-주파수 영역의 분석 방법인 위이블렛 변환을 결함진단 연구에 이용하였다. 웨이블렛 변환은 시간-주파수 영역에서 좋은 성능을 나타내며 다양한 기본 웨이블렛 함수를 가지고 있고 융통성과 적응력이 있는 변환방법과 해상도를 가지고 있어서, 급격히 변화하는 신호나 비정상 충격신호를 위치신호의 손실없이 잘 분석할 수 있는데, 이는 기어면에서 금속충돌이 일어나고 여거 기어이를 가지고 있는 복잡한 기어 시스템에서 더욱 유용하게 쓰일 수 있다. 또한 웨이블렛이 가진 주요 특성 중 하나인 잡음제거(de-noise) 기능을 사용하여 잡음이 합쳐져 있는 실제 신호를 더욱 효과적으로 분석 할 수 있었다. 실험대상은 두 쌍의 기어 물림이 있는 기어구동계를 사용하였고, 먼저 실험대상인 기어열에서 이론적으로 발생할 수 있는 싸인파들의 합성으로 이루어진 신호를 가지고 시뮬레이션을 실시하였고, 실험장치를 통해 정상상태기어에서의 신호와 기어에 인위적으로 발생시틴 결함에 의해 발생되는 신호를 측정하였으며 이러한 신호를 웨이블렛 변환하는 방법을 통해 결함을 분석하였다. In this paper, the problems of the fault detection of gear trains are considered, and the wavelet transforms are applied to evaluate the performance of detecting the incipient gear damage with real experimental data. The wavelet transform enables one to look at the evolution in time of a signal's frequency content. This property make it very suitable for the detection of transient vibrations generated by developing localized faults in gear trains. Recent applications of the wavelet technique have focused on the use of DWT(Descret Wavelet Transform) coefficients only. In this study, the wavelet denosing is also applied to remove the effect of the noise in the measured vibration signals. The denoised wavelet map is found to display distinctive features in the presence of several kinds of fault in a tooth.

      • 관상동맥질환의 중증도와 혈중지질치 및 아포지단백과의 상관관계

        임현주,황종현,류재근,정병천,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

        목적 : 관상동맥질환의 위험인자로서 혈청지질 및 혈장 지단백에 대한 관심이 높아지고 있고 또 아포지단백이 관상동맥 질환을 일으키는데 다른 지질보다 더 중요하다는 연구가 많이 발표되고 있으나 우리나라에서는 아포지단백에 대한 연구가 그리 많지 않은 편이다. 이에 저자등은 혈중지질치와 새로운 위험인자로 알려지고 있는 아포지단백치를 측정하고 이들과 관상동맥질환 중증도와의 상관 관계를 알아보았다. 대상 및 방법 : 관상동맥질환이 의심되어 관상동맥조영술을 실시한 69명의 환자를 대상으로 하였으며 12시간 이상 공복후 혈청을 채취하여 총콜레스테롤, 중성지방, HDL-콜레스테롤, 아포지단백 AI (ape AI), 아포지단백 B (ape B)를 측정하였다. 관상동맥조영상 50%이상의 협착이 있는 혈관의 수 (lesion number), 최대협착을 합한 값 (lesion score), 각 분절의 최대협착을 합한 값 (total score), 가장 심한 협착 값 (peak stenosis) 등으로 관상동맥 중증도를 알아본 다음 이들과 혈청지질치, 아포지단백과의 상관 관계를 비교해 보았다. 상관 관계는 Spearman's correlation coefficient를 구하였다. 결과 : 관상동맥질환의 중증도와 각 지질치 간의 상관 관계를 비교해 보았을때 lesion number와 관계있는 것은 연령 (r=0.2789), 중성지방치 (r=0.2829)이며 lesion score와 관계있는 것은 연령(r=0.2911), 중성지방치0 (r=0.3316), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2361), apo B 치(r=0.2759), apo AI과 apo B의 비 (-0.3154)로 나타났다. Total score는 연령 (r=0.3100), 총콜레스테롤치 (r=0.2452), 중성지방치 (r=0.3022), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2770), apo B치 (r=0.2927), apo AI과 apo B의 비 (r=-0.3408)와 연관이 있었고 peak stenosis는 중성지방 치(r=0.3305), ape B 치 (r=0.2968), apo AI과 apo B의 비 (r=-0.3977)와 관계가 있었다. 연령으로 조정하여 partial correlation을 좌면, 관상동맥조영술상 중증도는 중성지방치 (r=0.3408), apo B 치(r=0.2877), apo AI과 apo B의 비 (r=-0.3460)와 유의한 상관 관계가 있었다. 이중 ape AI과 ape B의 비는 lesion score (r=-0.2640), total score (r=-0.3057), peak stenosis (r=-0.3460)와 유의한 상관관계를 보였다. 결론 : 이상의 결과로 보아 apo B치 그리고 apo AI과 apo B의 비가 관상동맥 중증도의 예견인자로 다른 혈중 지질 치보다 더 유용하다는 것을 알 수 있었다. Objectives : Recently, it was reported that the measurements of apolipoprotein levels may be valuable in the clinical assessment of coronary artery disease severity. However there are a few reports regarding to the relationship between coronary artery disease severity and apolipoprotein levels in Korea. Thus, we measured serum lipid and apolipoprotein levels and studied the relationship between coronary artery disease severity and serum lipids and apolipoproteins levels. Subjects and Methods : The 69 patients who underwent coronary angiography to evaluate chest pain were subjected to this study. We measured the levels of serum cholesterol, triglycerides, HDL cholesterol, apolipoprotein AI(apo AI) and apolipoprotein B(Apo B) and measured the severity of coronary artery disease by lesion number, lesion score, total score and peak stenosis. Then, we studied the relationship between coronary artery disease severity and apolipoproteins and calculated the correlation coefficient. Results : The results were as follows. There are significant correlation(r=0.3) between age, triglycerides, apo B levels, apo AI/B ratio and coronary artery disease severity. In controlling for age, tyiglycerides, apo B levels and apo AI/B ratio shows significant correlation(r=0.3) with coronary artery disease severity. Especially, apo AI/B ratio shows significant correlation(r=0.3) with lesion number, total score and peak stenosis. Conclusion : These results suggest that the apo B levels and the ratio of apo AI/B can be used as significant independent predictor for coronary artery disease severity rather than other serum lipid levels.

      • 경북지역 주부들의 전통 부엌 세간의 보유 현황 및 이용실태 조사 연구

        한재숙,최영희,조연숙,변재옥,한경필,김현옥,정종기,최석현 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.4

        Housewives residing in the Kyongsang-Buk-do area were surveyed to determine their ownership of Korean traditional kitchen appliances and their usages. A breakdown of the appliances for the survey was as follows: 16 tableware, 17 household utensils, 15 heating utensils, 14 ceramic and earthen pottery, 6 stone utensils, 9 cooking utensils, 9 utensils made of the dried bamboo and bush clover, and 13 dining tables ("sang"). The types of the appliances with the largest ownerships and most frequently used were as follows: The jeopsi was most frequently owned, followed by the daejeop and the jaengban. The most frequently used item was the jubal,, followed by the daejeop and the jeopsi. Among the wooden utensils, bangmangi was most frequently owned, followed by the chanjang, the che, the doe, mal and hop. The most frequently used household utensil was the chanjang, followed by the takjasang and the doe, mal and hop. The utensil the most people owned for heating was the jujeonja, followed by the seoksoe, the musoesot and the siru. The most frequently used utensil for heating was the jujeonja, the musoesot and the seoksoe, in the order. As for the ceramic and earthen pottery, hangari and dok were owned and used most frequently. The maetdol and the jeolgu, though very low in their ownership rate, were most frequently owned items among the stoned utensils. The kal and the doma were the most frequently owned and used cooking utensils. The sokuri, and the chaeban and the baguni were the most frequently owned among the utensils made of the dried bamboo, bush clover and straw, while the sokuri was used the most frequently, followed by the chaeban and the jori. Among the dining tables, the kyojasang was the item most frequently owned, followed by the seonban and the chaeksangban, while the wonban was the most frequently used, followed by the kyojasang and the chaeksangban.

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