Antioxidant activity of Moringa oleifera seed extracts

Ismet Ara Jahan,Md. Hemayet Hossain,Khondoker Shahin Ahmed,Zakia Sultana,Pizush Kanti Biswas,Katrun Nada 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.4

The present research evaluates the phytochemical and antioxidant activity of Moringa oleifera Lam. seed kernel grown in Bangladesh. M. oleifera seed kernel was extracted with methanol, acetone and water individually. The phytochemical content was evaluated through the determination of total phenolic, total flavonoid and total tannin contents. In vitro antioxidant capacity was determined following four complementary methods: DPPH (2,2-diphenyl-1-picrylhydrazyl, ABTS{2,2′-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid)}, and NO (nitric oxide) free radical scavenging and reducing power tests. The total phenolic contents 60.99 ± 0.153, 30.78 ± 0.101 and 90.97 ± 0.134 mg gallic acid eq./g dry extract, total flavonoids contents 10.13 ± 0.171, 13.32 ± 0.101 and 221.76 ± 0.221 mg quercetin eq./g and total tannins contents 97.10 ± 0.153, 73.91 ± 0.107 and 21.74 ± 0.086 mg tannic acid eq./g dry extract were found to be present in methanol, acetone and water extracts respectively. Among the three extracts, the water extract exhibited significant activities for scavenging DPPH, ABTS and NO free radicals with EC50 values 36.89 ± 0.154, 13.20 ± 0.049 and 217.95 ± 0.327 μg/mL respectively against the standard antioxidant compound ascorbic acid and butylated hydroxy anisole. The results of this research revealed that among the three extracts of M. oleifera seed kernel the water extract exhibited significant free radical scavenging activity. Reducing power, total phenolic and total flavonoid contents were also observed to be significant for the water extract. So in contrast of all the results of this study it can be concluded that among the three extracts (methanol, acetone and water) the water extract possessed potent antioxidant activity which support the use of M. oleifera seed kernel as natural antioxidant.

Evaluation of anti-inflammatory and antioxidant potential of the kernel root of Xylocarpus mekongensis (Lamk.) M. Roem

Jahan, Ismet Ara,Hossain, Md. Hemayet,Nimmi, Ishrat,Islam, Siblara,Kawsar, Md. Hassan,Islam, Md. Amirul 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.3

Anti-inflammatory and antioxidant activities of the crude ethanolic extract of the kernel root of Xylocarpus mekongenesis (Lamk.) M. Roem. (Meliaceae) was evaluated in different methods. The extract showed significant antiinflammatory activity in carrageenan-induced oedema test in rats showing 69.42 % reduction in the paw volume (P<0.01) comparable to that produced by the standard drug indomethacin (70.25 %) at 24 h when the extract was fed at the dose of 400 mg/kg body weight. The percentage inhibition of the oedema paw volume was statistically significant (p<0.05) when compared with the indomethacin treated animals at 0.5, 1, 2, 4 and 6 h. The extract exhibited $IC_{50}$ value 4.78 ${\mu}g/ml$ in DPPH free radical scavenging activity test that was found to be very significant and comparable to that of the standard antioxidants ascorbic acid and BHA (1.9372 and 4.81 ${\mu}g/ml$) respectively. In nitric oxide radical scavenging test, the $IC_{50}$ value was also found fairly significant 57.37 ${\mu}g/ml$ when compared to standard ascorbic acid (36.81 ${\mu}g/ml$). In reducing power test, the maximum absorbance of the same extract was observed as 1.923 at 100 ${\mu}g/ml$ and that of ascorbic acid and BHA were 2.8111 and 1.0314 respectively. The extract showed highest antioxidant capacity at 100 ${\mu}g/ml$ concentration expressed as equivalents of ascorbic acid (1.44). The total phenolic content of the extract was also found quite high ($342.15{\pm}0.92$ mg/g of gallic acid equivalent). Therefore, the results of this study suggest the probable anti-inflammatory and antioxidant activities of the ethanolic extract of X. mekongenesis kernel root and justify its use in traditional medicines.

Anti-inflammatory and antioxidant activities of the ethanolic extract of $Ceriops$ $decandra$ (Griff.) Ding Hou bark

Hossain, Hemayet,Moniruzzaman, Sk.,Nimmi, Ishrat,Kawsar, Hassan,Hossain, Akbor,Islam, Amirul,Jahan, Ismet Ara 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.4

The crude ethanolic extract of the bark of $Ceriops$ $decandra$ (Griff.) Ding Hou (Family: Rhizophoraceae) was evaluated for its possible anti-inflammatory and antioxidant activities in different methods. At the dose of 400 mg/kg body weight, the extract showed a significant anti-inflammatory activity in the carrageenan-induced oedema test in rats showing 67.72% reduction in the paw volume ($P$ <0.01) comparable to that produced by the standard drug indomethacin (69.29%) at 24 h. The percentage inhibition of the oedema paw volume by the 400 mg/kg body weight of the extract was also statistically significant ($^*P$ <0.05 and $^{**}p$ <0.01) compared favorably with the indomethacin treated animals at 1, 2, 4, 6 and 24 h. Three complementary test systems for determining the antioxidant activities of the ethanolic extract of $C.$ $decandra$, namely DPPH free radical scavenging, reducing power, total phenolic contents determination were used for the analysis. In DPPH free radical scavenging test, $IC_{50}$ value for the ethanolic crude extract was found fairly significant (12.90 ${\mu}g$/ml) while compared to the $IC_{50}$ value of the reference standards ascorbic acid and butylated hydroxy anisole (2.9372 and 4.81 ${\mu}g$/ml) respectively. In reducing power test the absorbance for ethanolic extract was found to be 1.0900 at 100 ${\mu}g$/ml concentration while compared to 2.8111 and 1.0314 for standard ascorbic acid and BHA respectively at the same concentration. The amount of total phenolic content was also determined and calculated as quite high in the ethanolic extract (309.86 mg/g of gallic acid equivalent). Therefore, the obtained results tend to suggest the probable anti-inflammatory and antioxidant activities of the ethanolic extract of $C.$ $decandra$ bark and justify its use in folkloric remedies.

Anti-inflammatory and antioxidant activities of the ethanolic extract of Ceriops decandra (Griff.) Ding Hou bark

Hemayet Hossain,Sk. Moniruzzaman,Ishrat Nimmi,Hassan Kawsar,Akbor Hossain,Amirul Islam,Ismet Ara Jahan 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.4

The crude ethanolic extract of the bark of Ceriops decandra (Griff.) Ding Hou (Family: Rhizophoraceae)was evaluated for its possible anti-inflammatory and antioxidant activities in different methods. At the dose of 400 mg/kg body weight, the extract showed a significant anti-inflammatory activity in the carrageenan-induced oedema test in rats showing 67.72% reduction in the paw volume (P<0.01) comparable to that produced by the standard drug indomethacin (69.29%) at 24 h. The percentage inhibition of the oedema paw volume by the 400 mg/kg body weight of the extract was also statistically significant (*P<0.05 and **p<0.01) compared favorably with the indomethacin treated animals at 1, 2, 4, 6 and 24 h. Three complementary test systems for determining the antioxidant activities of the ethanolic extract of C. decandra,namely DPPH free radical scavenging, reducing power, total phenolic contents determination were used for the analysis. In DPPH free radical scavenging test, IC_(50) value for the ethanolic crude extract was found fairly significant (12.90 μg/ml) while compared to the IC_(50) value of the reference standards ascorbic acid and butylated hydroxy anisole (2.9372 and 4.81 μg/ml) respectively. In reducing power test the absorbance for ethanolic extract was found to be 1.0900 at 100 μg/ml concentration while compared to 2.8111 and 1.0314 for standard ascorbic acid and BHA respectively at the same concentration. The amount of total phenolic content was also determined and calculated as quite high in the ethanolic extract (309.86 mg/g of gallic acid equivalent). Therefore, the obtained results tend to suggest the probable anti-inflammatory and antioxidant activities of the ethanolic extract of C. decandra bark and justify its use in folkloric remedies.

Phenolic Content Analysis of Aloe vera Gel and Evaluation of the Effect of Aloe Gel Supplementation on Oxidative Stress and Fibrosis in Isoproterenol-Administered Cardiac Damage in Rats

Farzana Akther Sumi,Biswajit Sikder,Md Mizanur Rahman,Shamshad Rahman Lubna,Anayt Ulla,Md. Hemayet Hossain,Ismet Ara Jahan,Md Ashraful Alam,Nusrat Subhan 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.3

We evaluated the cardioprotective effect of Aloe vera gel isoprenaline (ISO)-administered myocardial infarction in rats. ISO administration increased lipid peroxidation and oxidative stress in rats, which were ameliorated by A. vera gel supplementation. Our study also revealed that creatine kinase-MB (CK-MB) activities were increased in ISO-administered rats, while the activities of cellular antioxidants, such as superoxide dismutase and catalase, and glutathione concentration were decreased. A. vera gel lowered CK-MB enzyme activities and the glutathione concentration in ISO-administered rats, and increased antioxidant activities. Histopathological examination also revealed increases in thickness of the left ventricle myocardium, increases in mononuclear cell infiltrations, increased degeneration of focal areas of the endocardium, and increased fibrous tissue deposition in the heart of ISO-administered rats; whereas, A. vera prevented infiltration of inflammatory cells and reduced left ventricular fibrosis. In conclusion, we show that A. vera supplementation protects against development of cardiac inflammation, fibrosis, and oxidative stress in ISO-administered rats.

Phenolic Content Analysis of Aloe vera Gel and Evaluation of the Effect of Aloe Gel Supplementation on Oxidative Stress and Fibrosis in Isoprenaline-Administered Cardiac Damage in Rats

Farzana Akther Sumi,Biswajit Sikder,Md Mizanur Rahman,Shamshad Rahman Lubna,Anayt Ulla,Md Hemayet Hossain,Ismet Ara Jahan,Md Ashraful Alam,Nusrat Subhan 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.3

We evaluated the cardioprotective effect of Aloe vera gel isoprenaline (ISO)-administered myocardial infarction in rats. ISO administration increased lipid peroxidation and oxidative stress in rats, which were ameliorated by A. vera gel supplementation. Our study also revealed that creatine kinase-MB (CK-MB) activities were increased in ISO-administered rats, while the activities of cellular antioxidants, such as superoxide dismutase and catalase, and glutathione concentration were decreased. A. vera gel lowered CK-MB enzyme activities and the glutathione concentration in ISO-administered rats, and increased antioxidant activities. Histopathological examination also revealed increases in thickness of the left ventricle myocardium, increases in mononuclear cell infiltrations, increased degeneration of focal areas of the endocardium, and increased fibrous tissue deposition in the heart of ISO-administered rats; whereas, A. vera prevented infiltration of inflammatory cells and reduced left ventricular fibrosis. In conclusion, we show that A. vera supplementation protects against development of cardiac inflammation, fibrosis, and oxidative stress in ISO-administered rats.