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        Is the Biochemical Mechanism of Petal Senescence Similar Within a Genus? A Case Study of Dianthus.

        Riyaz Ahmad Dar,Inayatullah Tahir,Syed Sabhi Ahmad 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.5

        Physiological and biochemical changes were documented during various stages (I-VI) of flower development and senescence in Dianthus barbatus L. and Dianthus chinensis L. A comparison between various biochemical parameters revealed that different biomolecules show different trends during senescence in these two species. Although floral diameter, fresh mass, dry mass and water content showed a positive relationship with flower opening and a sharp decline with senescence in both species; soluble proteins, α-amino acids and phenols showed a significant increase towards opening and a decrease towards senescence in D. chinensis but a reverse trend was observed in D. barbatus. Specific protease activity in D. chinensis showed a decrease towards flower opening and an increase towards senescence, but in D. barbatus, specific protease activity increased continuously from bud formation to senescence. Total sugars increased with flower opening and decreased as senescence progressed in both species. Reducing sugars increased as the flowers of D. chinensis opened and declined towards senescence, but in D. barbatus, reducing sugars remained almost constant from flower opening to senescence. To summarize, the various physiological and biochemical changes that execute senescence vary within a genus.

      • Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan

        Tahira, Bibi,Asif, Muhammad,Khan, Samiullah,Hussain, Abrar,Shahwani, Muhammad Naeem,Malik, Arif,Inayatullah, Syed,Iqbal, Zafar,Rasool, Mahmood Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

        Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome. Materials and Methods: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms. Results: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 $10^3/{\mu}l$, hemoglobin 10.44 mg/dl, and platelets 288.6 $10^3/{\mu}l$ were observed in this study. Conclusions: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.

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