The Effect of Rosiglitazone on the Cell Proliferation and the Expressions of p27 and Skp2 in<i>Helicobacter pylori</i>Infected Human Gastric Epithelial Cells

Kim, Sung-Soo,Cho, Young-Seok,Kim, Hyung-Keun,Shin, Ok-Ran,Chae, Hiun-Suk,Choi, Myung-Gyu,Chung, In-Sik The Korean Society of Gastroenterology 2010 대한소화기학회지 Vol.55 No.4

<P>Background/Aims: Ligands for peroxisome proliferator-activated receptorgamma (PPARgamma), a member of the ligand-activated nuclear receptor superfamily, exhibit anti-tumoral effects and are associated with de novo synthesis of proteins involved in regulating the cell cycle and cell survival/death. Helicobacter pylori (H. pylori) is an etiologic agent for gastric adenocarcinoma, and raises the cell turnover of gastric epithelium. The aim of this study was to investigate the effect of PPARgamma ligand rosiglitazone on the cell proliferation and the expressions of p27 and Skp2 protein in H. pylori infected gastric epithelial cells. Methods: We examined the expression of PPARgamma by Western blot in H. pylori infected AGS human gastric epithelial cells. The effect of rosiglitazone on the survival of H. pylori infected AGS cells was assessed by cell viability assay. After the treatment of rosiglitazone in H. pylori infected AGS cells, the expressions of p27 and Skp2 were assessed by Western blot. Results: The expression of PPARgamma protein was increased in H. pylori infected AGS cells. Cell growth was inhibited and decreased in dose- and time- dependent manner in H. pylori infected AGS cells treated with rosiglitazone. A decrease in Skp2 expression and a reciprocal increase in p27 expression were found in dose- and time-dependent manner in H. pylori infected AGS cells treated with rosiglitazone. Conclusions: Rosiglitazone inhibited the growth of H. pylori infected AGS cells. Rosiglitazone attenuated Skp2 expression, thereby promoting p27 accumulation in H. pylori infected human gastric epithelial cells. Further studies will be needed to find the effects of accumulation on cell turnover in H. pylori infection and the role in the H. pylori-associated gastric carcinogenesis.</P>

순산소 순환유동층에서 로내 탈황 및 탈질법 적용에 따른 오염물질 거동특성

최경구(Gyung-Goo Choi),나건수(Geon-Soo Na),신지훈(Ji-Hoon Shin),길상인(Sang-In Keel),이정규(Jung-Kyu Lee),허필우(Pil-Woo Heo),윤진한(Jin-Han Yun) 한국청정기술학회 2018 청정기술 Vol.24 No.3

순산소 연소기술은 화력발전에 적용 가능한 유망한 온실가스 감축 기술로 평가되고 있다. 본 연구는 환경적 관점에서 순환유동층을 활용한 순산소 연소조건에 로 내 탈황 및 탈질법을 적용하여 NO 및 SO₂의 거동을 살펴보는 한편, SO₃, NH₃, 그리고 N2O의 발생 경향도 관측하였다. 이를 위해, 연소로 내 석회석 및 요소수를 투입하였다. 로 내 탈황법은 연소가스 내 SO₂ 농도를 ~403에서 ~41 ppm까지 저감하였다. 또한 SO₃ 형성의 주원료인 SO₂가 저감되면서 연소가스 내 SO₃ 농도도 ~3.9에서~1.4 ppm까지 감소되었다. 그러나 석회석 내 CaCO₃가 NO의 발생을 촉진하는 현상도 관측되었다. 연소가스 내 NO 농도는로 내 탈질법을 적용하여 ~26 - 34 ppm까지 저감되었다. 요소수 투입량 증가에 따라 연소가스 내 NH₃ 농도가 증가하여 최대~1.8 ppm으로 나타났으며, N₂O의 농도도 ~61에서 ~156 ppm까지 증가하였다. N₂O 발생량 증가 현상은 요소수의 열분해 과정에서 생성된 HNCO가 N₂O로 전환되어 나타난다. 본 연구의 결과를 통해 로 내 연소가스 세정법을 적용할 경우 NOx 및 SOx의 저감뿐만 아니라, 다른 오염물질의 발생에 대한 주의가 필요할 것으로 보인다. Oxy-fuel combustion is considered as a promising greenhouse gas reduction technology in power plant. In this study, the behaviors of NO and SO₂ were investigated under the condition that in-furnace deNOx and deSOx methods are applied in oxy-fuel circulating fluidized bed combustion condition. In addition, the generation trends of SO₃, NH₃ and N2O were observed. For the purpose, limestone and urea solution were directly injected into the circulating fluidized bed combustor. The in-furnace deSOx method using limestone could reduce the SO₂ concentration in exhaust gas from ~403 to ~41 ppm. At the same experimental condition, the SO₃ concentration in exhaust gas was also reduced from ~3.9 to ~1.4 ppm. This trend is mainly due to the reduction of SO₂. The SO₂ is the main source of the formation of SO₃. The negative effect of CaCO₃ in limestone, however, was also appeared that it promotes the NO generation. The NO concentration in exhaust gas reduced to ~26 - 34 ppm by appling selective non-catalytic reduction method using urea solution. The NH₃ concentration in exhaust gas was appeared up to ~1.8 ppm during injection of urea solution. At the same time, the N₂O generation also increased with increase of urea solution injection. It seems that the HNCO generated from pyrolysis of urea converted into N₂O in combustion atmosphere. From the results in this study, the generation of other pollutants should be checked as the in-furnace deNOx and deSOx methods are applied.

급성 중독 환자에 대한 역학적 고찰 : 서울과 지방의 비교

김성중,김인병,최성욱,김경수,이영수,Choi, Jong Moo 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Study objective: To compare patients with acute poisoning between urban and rural areas Design: Retrospective chart review of 217 patients with acute poisoning Setting: Two University Hospital EDs Methods: patients were divided into two groups according to hospital. The group A was 109 patients who came to ED of Asan Medical Center and the group B was patients who came to ED of Ajou University Hospital from June 1994 to February 1995. We analysed the age and sex distributions, poisoning substances. causes of poisoning and the past history of psychiatric problems in patients of the two groups. Results: Total number of patients with acute poisoning were 109 in the group A and 108 in the group B. Female was more prevalent than male in the group A and male was more than female in the group B. There was a significant difference in sex distribution between two groups. The mean age of the group A was 27.7 years which was significantly different from the mean age of the group B, 33.7 years. The age incidences in both group were highest in 2nd decade. The accidental poisoning cases were 37% in the group A and 31% in the group B. The rates of committed suicide were 7% in the group A and 22% in the group B. The most common agent of poisoning was therapeutic drug in the group A and agricultural or industrial chemical in the group B. The overall mortality was 5% in the group A and 15% in the group B. Conclusion: There were significant differences in age and sex distributions. poisoning substances, causes of poisoning and the past history of psychiatric problems between two groups.

Regulation of Hepatic Gluconeogenesis by an ER-Bound Transcription Factor, CREBH

Lee, Min-Woo,Chanda, Dipanjan,Yang, Jianqi,Oh, Hyunhee,Kim, Su Sung,Yoon, Young-Sil,Hong, Sungpyo,Park, Keun-Gyu,Lee, In-Kyu,Choi, Cheol Soo,Hanson, Richard W.,Choi, Hueng-Sik,Koo, Seung-Hoi Elsevier 2010 Cell metabolism Vol.11 No.4

<P><B>Summary</B></P><P>Endoplasmic reticulum (ER)-bound transcription factor families are shown to be involved in the control of various metabolic pathways. Here, we report a critical function of ER-bound transcription factor, CREBH, in the regulation of hepatic gluconeogenesis. Expression of CREBH is markedly induced by fasting or in the insulin-resistant state in rodents in a dexamethasone- and PGC-1α-dependent manner, which results in the accumulation of active nuclear form of CREBH (CREBH-N). Overexpression of constitutively active CREBH activates transcription of <I>PEPCK-C</I> or <I>G6Pase</I> by binding to its enhancer site that is distinct from the well-characterized CREB/CRTC2 regulatory sequences in vivo. Of interest, knockdown of CREBH in liver significantly reduces blood glucose levels without altering expression of genes involved in the ER stress signaling cascades in mice. These data suggest a crucial role for CREBH in the regulation of hepatic glucose metabolism in mammals.</P> <P><B>Highlights</B></P><P>► PGC-1α/GR activates CREBH expression under fasting or insulin-resistant conditions ► CREBH enhances hepatic gluconeogenesis via a CRTC2-dependent manner ► Depletion of CREBH in the liver ameliorates fasting hyperglycemia in diabetic mice</P>

수리된 복합레진 수복물의 전달결합강도 연구

최수영,정선와,황윤찬,김선호,윤창,오원만,황인남 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.6

This study was performed to evaluate the interfacial shear bond strength of base (direct and indirect) and repair composites with aging and surface treatment methods. Direct composites resin specimens (Charisma®, Heraeus Kulzer, Germany) were aged for 5 min, 1 hour, 24 hours, and 1 week in 37℃ distilled water before surface treatment, and then divided into five groups: Group 1, grinding; Group 2, grinding and application of bonding agent; Group 3, grinding, etching with 37% phosphoric acid for 30sec, and application of bonding agent; Group 4, grinding, etching with 37% phosphoric acid for 30sec, silane treatment, and application of bonding agent; Group 5, grinding, etching with 4% hydrofluoric acid for 30sec, silane treatment, and application of bonding agent. Indirect composite resin specimens (Artglass®, Heraeus Kulzer, Germany) were aged for 1 week in 37℃ distilled water and divided into seven groups: Group 1 - Group 5, equal to Charisma specimens; Group 6, grinding, etching 37% phosphoric acid for 60sec, silane treatment, and application of bonding agent; Group 7, grinding, etching with 4% hydrofluoric acid for 60 sec, silane treatment, and application of bonding agent. The repair material(Charisma®) was then added on the center of the surface (5mm in diameter, 5mm in height). The shear bond strength was tested and the data was analyzed using one-way ANOVA and the Student-Newman-Keuls test. The following conclusions were drawn. 1. The shear bond strength of Charisma® specimens aged for 1 hour was significantly higher in Group 2 and Group 5 than in Group 1 (p<0.05), and that of Charisma® specimens aged for 1 week was significantly higher in Group 3 and Group 5 than in Group 1 (p<0.05). No significant difference was found in the bond strength of specimens aged for 5 min and 24 hours. 2. In Group 2 of the Charisma® specimens, there was significant difference between the bond strength of 24 hours and that of 1 week (p<0.05). 3. In Group 4 of the Charisma® specimens, the shear bond strength of specimens aged for 24 hours was significantly higher than the others(p<0.05). 4. There was no significant diiference between the shear bond strength of the Artglass® specimens. 5. Most of the Charisma® specimens showed cohesive fractures. Artglass® specimens that were etched with acid(phosphoric or hydrofluoric) for 30 sec showed more cohesive fractures.

In-hospital mortality in patients with STEMI admitted during off hours

( Sung-Soo Kim ),( Myung Ho Jeong ),( Jeong Gwan Cho ),( Young Keun Ahn ),( Jong Hyun Kim ),( Shung Chull Chae ),( Young Jo Kim ),( Seung Ho Hur ),( In Whan Seong ),( Taek Jong Hong ),( Dong-hoon Choi 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Conflicting results exist on the outcome of off hours PCI in ST elevation myocardial infarction (STEMI). However, there were only a few studies that have focused on the clinical characteristics and outcomes of off hours PCI in STEMI. So, we studied the clinical characteristics and hospital mortality in STEMI patients treated with primary PCI during regular hours (weekdays 9:00 AM to 6:00 PM) versus off hours Weekdays 6:01 PM to 8:59 AM, weekends, and holidays) in Korea Acute Myocardial Infarction Registry. We analyzed in hospital and one year mortality among 5,665 consecutive ST segment elevation myocardial infarction patients treated with primary PCI between November 2005 to January 2008. Total 2,848 (50.2%) patients were treated during off hours. Baseline finding were similar, although regular hours patients were older. Median symptom to balloon time (304 min, IQR 175 to 750 vs. 270 min, IQR 145 to 551, p=0.001) were longer for regular hours primary PCI. Median door to balloon time (71min, IQR: 48 to 132 vs. 59 min, IQR 39 to 110 min, p=0.001) were longer for off hours pPCI. Also, Cardiac enzyme such as Max CK-MB (212.1±299.3 vs 194.7±303.4, p=0.031) and max TnI (72.6±239.5 vs. 58.9±94.4, p=0.013) were increased in off hours pPCI. However, unadjusted in hospital (6.0% off hours vs. 6.0% regular hours, p=0.946) and one year cardiac mortality (11.3% off hours vs. 11.7% regular hours, p=0.661) were comparable. In multivariate analysis, off hours primary PCI did not predict an adverse outcome. In conclusion, when primary PCI was performed within an appropriate reperfusion strategy, the clinical effectiveness of either off hours or regular hours pPCI is comparable.

수리된 복합레진 수복물의 전단결합강도 연구

최수영,오원만,황인남 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.1

This study was performed to evaluate the interfacial shear bond strength of base (direct and indirect) and repair composites with aging and surface treatment methods. Direct composite resin specimens (Charisma^(r), Heraeus Kulzer, Germany) were aged for 5min, 1hour, 24hour, and 1week in 37℃ distilled water before surface treatment, and then divided into five groups:Group 1, grinding; Group 2, grinding and application of bonding agent;Group 3, grinding, etching with 37% phosphoric acid for 30sec, and application of bonding agent;Group 4, grinding, etching with 37% phosphoric acid for 30sec, silane treatment, and application of bonding agent;Group 5, grinding, etching with 4% hydrofluoric acid for 30sec, silane treatment, and application of bonding agent. Indirect composite resin specimens (Artglass^(r), Heraeus Kulzer, Germany) were aged for 1week in 37℃ distilled water and divided into seven groups:Group 1-Group 5, equal to Charisma specimens;Group 6, grinding, etching with 37% phosphoric acid for 60sec, silane treatment, and application of bonding agent;Group 7, grinding, etching with 4% hydrofluoric acid for 60sec, silane treatment, and application of bonding agent. The repair material(Charisma^(r)) was then added on the center of the surface (5mm in diameter, 5mm in height). The shear bond strength was tested and the data was analyzed using one-way ANOVA and the Student-Newman-Keuls test. The following conclusions were drawn. 1. The shear bond strength of Charisma^(r) specimens aged for 1hour was significantly higher in Group 2 and Group 5 than in Group 1 (p<0.05), and that of Charisma^(r) specimens aged for 1week was significantly higher in Group 3 and Group 5 than in Group 1 (p<0.05). No significant difference was found in the bond strength of specimens aged for 5min and 24hours. 2. In Group 2 of the Charisma^(r) specimens, there was significant difference between the bond strength of 24hours and that of 1week (p<0.05). 3. In Group 4 of the Charisma^(r) specimens, the shear bond strength of specimens aged for 24hours was significantly higher than the others(p<0.05). 4. There was no significant difference between the shear bond strength of the Artglass^(r) specimens. 5. Most of the Charisma^(r) specimens showed cohesive fractures. Artglass^(r) specimens that were etched with acid (phosphoric or hydrofluoric) for 30sec showed more cohesive fractures.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

氏族마을의 儒敎文化景觀 特性에 관한 연구 : 상주시 우산리를 사례로 in the Case of Usan Village in Sangju City

백인권,최기수 한국정원학회 2002 한국전통조경학회지 Vol.20 No.3

This study basically assumes that housing form is influenced by social/cultural backgrounds rather than natural circumstances. The most significant characteristic in villages, originated in Chosun dynasty, is that village people are linked with lineage. With this point of view, I focused on Usan-ri Sangju-si, Gyeongsangbuk-do, where famous Song Confucianism scholar Jung, Gyungse (鄭經世 , with the pen name of Ubok. 1563-1633)'s family had settled down. The purpose of the study is to find out both the general and specific characteristics of physical structure and cultural landscape in this lineage village, based on its planning and development process of the village. For the study, I focused on the gentry's personal design ideas as well as Song Confucianism's influence on buildings of the village. Sang-Usan village had been transformed since Ubok has lived in Usan village; cultural place(修己空間) →residential place(居住空間)→ceremonial place(儀禮空間). The pavilion(聽癎亭) has a simple structure without a kitchen, which means Ubok used the house for a limited purpose (as a studying and mental training), not for living. After Usan area was granted by Yeongjo(英祖) as a Royal gift, the descendants moved to Usan for dwelling and took care of it as a special place. There is two things that need to be focused for planning a traditional village in Korea: finding a place for mental training and/or studying and a vantage place for living. The residential area was relatively small. The characteristics of physical formation of the village had been changed. When Ubok had settled down in the village for the first time, the major reason of locating a house was finding a place for the cultivation of the mind. However, when Jung, Joowon (鄭胄源, 1686-1756) moved into the Usan village for residential purpose, take a farm near by Usan area. Ubok's absolute sprit and power is easily found in the Usan-ri area. for example, most of Confucianism facilities - such as carved letters on a stone(刻宇), Usan-Seowon(愚山書院), and the household shrine(家廟祠堂) - are placed in Sang-Usan(上愚山) village and other residential functions are placed in Ha-Usan(下愚山) village.

수중 하베스트형 빙축열 시스템의 성능특성

최인수(In-Soo Choi),차동안(Dong-An Cha),김재돌(Jae-Dol Kim),윤정인(Jung-In Yoon) 한국마린엔지니어링학회 2003 한국마린엔지니어링학회 학술대회 논문집 Vol.2003 No.-

This paper is concerned with the development of a new method for making and separating ice and saving floated ice by installing an evaporation plate at in-water within a storage tank. In a conventional harvest-type ice storage system, a tank saves ice by separating a formed ice from an installed evaporation plate, which is located above an ice storage tank as an ice storage system. A new harvest-type method shows very good heat transfer efficiency than a convectional method. It is because the evaporation panel is directly contacted with water in .a storage tank. Also, at a conventional system a circulating pump, a circulating water distributor and a piping are installed, but these components are not necessary in a new method. The results showed discharge of cold capacity of new type indicated the high values about 30-40% based on five time of drive, the temperature difference of inlet/outlet occurred the big range about 1.3 ℃. So, the new type which makes ice in water showed superiorly.