Role of the Meckel’s Cartilage in Embryonic Mandibular Development of Mice

J. W Choi,S. B Han,J. H Sung,H. I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

Mecke!'s car t ilage is one of the ea rliest structu re to appear in a mandible derived from the lï rst branchi a l a rch and serves as the primorclium I"or the formation 01‘ mandible‘ mall eus. incus. and sphenomandibular li gament However. its direct role a nd the mechanism in mandibular clevelopment a re not well elucidated. 1'0 address t his Issue‘ we observed morphol ogical and histological changes and gene expression patterns in the Mecke!'s cart ilage 01" a cleveloping mouse. I"rom E13.5 to E18.5 embryos. using skeletal preparation samples a ncl routinely prepa red s lide secti ons for light mi croscopic observation in various sectional planes. The following methods were per |‘ ormecl : H&E staining I"or general hi st이 og i cal observation ‘ Von Kossafor detection of minerali zation. TRAP activ ity staining for locali zaLion 01’ osteoclastic cell s. immunohistochemistry for !Iα@-1 a ncl -9 forevaluati on of enzy matic activity 01" osteoclasLic cell s. a ncl in situ hybricli zation for detection of collagen type 1. Il. ancl X mRNA ex presslon‘ respecLively. AL E1 3.5 Mec kel's cartilage appeared as a V-shaped rod fused a t the micl line and thin minera li zed ma ndibular buccal plaLe was I"ormed lateral to. and at some clistance from. Meckel’s carti lage in an intramembranous ossi lïcation mocle. WiLh the progression of tooth development. t he Meckel’s in carti lage adjacent incisors revealecl hyperLrophi c chonclrocyte di ff"er entiation with minerali zation of the chondroid matrix. The Meckel’s car Li lage was replacecl with bone by o~ L eoc l asLs . showing strong immunoreact ivity for MMP- l ancl -9 from E16 5 Wi Lh ti me‘ Lhis bony replacement of Meckel's cartilage in an endochondral ossification mode was ex Lenclecl up Lo the mid-porLion of Lhe molar sockets til l EI8.5. The bony replacement of minera li zed hypertrophic chondrocyte zone expressing X collagen mHNA conLri buted to the formation of thick mandibular lingual plate . 1'hese f"i ndings suggesL LhaL mandibular formalion and development is closely relatecl with not only Mecke!'s carLi lage. buL also wiLh Lhc developing LooLh. and thaL C'erLai n in f"l uence from the developing tooth may play a role in detcrmin in g Lhe faLc of Meckel’s ca rLi lage cluring ma ndi bular development.

Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO₂

Sung, C.G.,Kim, T.W.,Park, Y.G.,Kang, S.G.,Inaba, K.,Shiba, K.,Choi, T.S.,Moon, S.D.,Litvin, S.,Lee, K.T.,Lee, J.S. Elsevier 2014 Journal of marine systems Vol.137 No.-

Since the Industrial Revolution, rising atmospheric CO<SUB>2</SUB> concentration has driven an increase in the partial pressure of CO<SUB>2</SUB> in seawater (pCO<SUB>2</SUB>), thus lowering ocean pH. We examined the separate effects of exposure of gametes to elevated pCO<SUB>2</SUB> and low pH on fertilization success of the sea urchin Strongylocentrotus nudus. Sperm and eggs were independently exposed to seawater with pCO<SUB>2</SUB> levels ranging from 380 (pH7.96-8.3) to 6000ppmv (pH7.15-7.20). When sperm were exposed, fertilization rate decreased drastically with increased pCO<SUB>2</SUB>, even at a concentration of 450ppmv (pH range: 7.94 to 7.96). Conversely, fertilization of Hemicentrotus pulcherrimus was not significantly changed even when sperm was exposed to pCO2 concentrations as high as 750ppmv. Exposure of S. nudus eggs to seawater with high pCO<SUB>2</SUB> did not affect fertilization success, suggesting that the effect of increased pCO<SUB>2</SUB> on sperm is responsible for reduced fertilization success. Surprisingly, this result was not related to sperm motility, which was insensitive to pCO<SUB>2</SUB>. When seawater was acidified using HCl, leaving pCO<SUB>2</SUB> constant, fertilization success in S. nudus remained high (>80%) until pH decreased to 7.3. While further studies are required to elucidate the physiological mechanism by which elevated pCO<SUB>2</SUB> impairs sperm and reduces S. nudus fertilization, this study suggests that in the foreseeable future, sea urchin survival may be threatened due to lower fertilization success driven by elevated pCO<SUB>2</SUB> rather than by decreased pH in seawater.

국내분리 S. pyogenes 균주에 대한 역학적 특성에 관한연구 (1)

이영희,주영란,황규잠,박강수,이광준,최영실,성화영,김기상 국립보건연구원 1999 국립보건원보 Vol.36 No.-

원발성 간암에서 PIVKA-II 및 Lens Culinaris Agglutinin-A 반응성 Alpha-fetoprotein(AFP-L3)의 임상적 유용성

배시현,박두호,양진모,박영민,차상복,최종영,성광용,조세현,정규원,선희식,김부성,최상욱,변병훈,한남익 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.2

Background/Aims: Des-γ-carboxy prothrombin(DCP), a protein induced by vitamin K absence or antagonist-II(PIVKA-II), and Lens culinaris agglutinin-A reactive AFP-L3 have been reported to be useful markers for the diagnosis of hepatocellular carcinoma (HCC). In the present study, both the PIVKA-II and AFP-L3 were analyzed and compared with a conventional AFP to determine its usefulness, specificity and sensitivity in the diagnosis of HCC. Methods: Sera were collected from 108 patients consisting of 17 patients with chronic hepatitis, 22 patients with liver cirrhosis and 69 patients with HCC. The AFP-L3 was determined by an lectin affinity electrophoresis coupled with an antibody-affinity blotting. Level of DCP was measured by an enzyme immunoassay with an anti-DCP monoclonal antibody. Results: The sensitivity and specificity of PIVKA-II and AFP L3 were 49.3% and 89.5%, and 32.5% and 85.7%, respectively. No significant correlation was found between the PIVKA-II or AFP L3 and serum AFP. No correlation was found etween the PIVKA-II or AFP L3 and the characteristics of HCC. Conclusion: The determination of plasma DCP and AFP L3 levels combined with AFP levels may be useful especially for the differential diagnosis between HCC and chronic liver diseases without HCC.(Korean J HepatoBackground/Aims: Des-γ-carboxy prothrombin(DCP), a protein induced by vitamin K absence or antagonist-II(PIVKA-II), and Lens culinaris agglutinin-A reactive AFP-L3 have been reported to be useful markers for the diagnosis of hepatocellular carcinoma (HCC). In the present study, both the PIVKA-II and AFP-L3 were analyzed and compared with a conventional AFP to determine its usefulness, specificity and sensitivity in the diagnosis of HCC. Methods: Sera were collected from 108 patients consisting of 17 patients with chronic hepatitis, 22 patients with liver cirrhosis and 69 patients with HCC. The AFP-L3 was determined by an lectin affinity electrophoresis coupled with an antibody-affinity blotting. Level of DCP was measured by an enzyme immunoassay with an anti-DCP monoclonal antibody. Results: The sensitivity and specificity of PIVKA-II and AFP-L3 were 49.3% and 89.5%, and 32.5% and 85.7%, respectively. No significant correlation was found between the PIVKA-II or AFP-L3 and serum AFP. No correlation was found etween the PIVKA-II or AFP L3 and the characteristics of HCC. Conclusion: The determination of plasma DCP and AFP-L3 levels combined with AFP levels may be useful especially for the differential diagnosis between HCC and chronic liver diseases without HCC.(Korean J Hepatol 2000;6:205-214)l 2000;6:205-214)

Potential Effect of S-Nitrosylated Protein Disulfide Isomerase on Mutant SOD1 Aggregation and Neuronal Cell Death in Amyotrophic Lateral Sclerosis

Jeon, G. S.,Nakamura, T.,Lee, J. S.,Choi, W. J.,Ahn, S. W.,Lee, K. W.,Sung, J. J.,Lipton, S. A. HUMANA PRESS INC 2014 Molecular neurobiology Vol.49 No.2

Aggregation of misfolded protein and resultant intracellular inclusion body formation are common hallmarks of mutant superoxide dismutase (mSOD1)-linked familial amyotrophic lateral sclerosis (FALS) and have been associated with the selective neuronal death. Protein disulfide isomerase (PDI) represents a family of enzymatic chaperones that can fold nascent and aberrant proteins in the endoplasmic reticulum (ER) lumen. Recently, our group found that S-nitrosylated PDI could contribute to protein misfolding and subsequent neuronal cell death. However, the exact role of PDI in the pathogenesis of ALS remains unclear. In this study, we propose that PDI attenuates aggregation of mutant/misfolded SOD1 and resultant neurotoxicity associated with ER stress. ER stress resulting in PDI dysfunction therefore provides a mechanistic link between deficits in molecular chaperones, accumulation of misfolded proteins, and neuronal death in neurodegenerative diseases. In contrast, S-nitrosylation of PDI inhibits its activity, increases mSOD1 aggregation, and increases neuronal cell death. Specifically, our data show that S-nitrosylation abrogates PDI-mediated attenuation of neuronal cell death triggered by thapsigargin. Biotin switch assays demonstrate S-nitrosylated PDI both in the spinal cords of SOD1 (G93A) mice and human patients with sporadic ALS. Therefore, denitrosylation of PDI may have therapeutic implications. Taken together, our results suggest a novel strategy involving PDI as a therapy to prevent mSOD1 aggregation and neuronal degeneration. Moreover, the data demonstrate that inactivation of PDI by S-nitrosylation occurs in both mSOD1-linked and sporadic forms of ALS in humans as well as mice.

Emergence of Panton-Valentine leucocidin-positive ST8-methicillin-resistant Staphylococcus aureus (USA300 clone) in Korea causing healthcare-associated and hospital-acquired bacteraemia

Jung, J.,Song, E. H.,Park, S. Y.,Lee, S. R.,Park, S. J.,Sung, H.,Kim, M. N.,Kim, S. H.,Lee, S. O.,Choi, S. H. Springer Science + Business Media 2016 European journal of clinical microbiology & infect Vol.35 No.8

<P>Panton-Valentine leucocidin (PVL)-positive sequence type (ST)8-MRSA-SCCmec IVa (USA300) is the epidemic strain of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in North America. USA300 is extremely rare in South Korea, and PVL-negative ST72 SCCmec type IVc is the predominant CA-MRSA clone. In a multicentre, prospective cohort study of S. aureus bacteraemia, we identified PVL-positive ST8-MRSA isolates by performing multilocus sequence typing and PCR for PVL. We analyzed the clinical characteristics of patients with PVL-positive ST8-MRSA bacteraemia, and performed SCCmec, spa, and agr typing, PCR for arginine catabolic mobile element (ACME), virulence gene profiling, and pulsed-field gel electrophoresis (PFGE). Among a total of 818 MRSA isolates, we identified ten isolates of PVL-positive ST8-MRSA (USA300) (3 from Hospital D, 4 from Hospital G, and 3 from Hospital A), all of which involved exclusively healthcare-associated (5 isolates) and hospital-acquired bacteraemia (5 isolates). This strain accounted for 8 similar to 10 % of the hospital-acquired MRSA bacteraemia in Hospitals D and G. Bacteraemia of unknown origin was the most common type of infection followed by pneumonia. All the isolates were SCCmec type IVa, spa type t008, and agr group I. Eight of the isolates harboured ACME. In a PFGE analysis, four isolates were identical to the USA300 control strain, five differed by a single band, and the remaining one differed by two bands. All the isolates were pulsed-field type USA300. This is the first report of healthcare-associated and hospital-acquired bacteraemia caused by USA300 in South Korea. USA300 seems to be an emerging hospital clone in this country.</P>

국내분리 Strptococcus pyogenes에 대한 표현형 및 유전형 분석 (Ⅱ)

이영희,황규잠,박강수,이광준,최영실,성화영,김기상 국립보건연구원 2000 국립보건원보 Vol.37 No.-

척추전방전위증 환자에서 Interlaminar Resin Fixation과 Interspinous Wiring

최영근,황정현,성주경,황성규,함인석,박연묵,김승래 慶北大學校 醫科大學 1994 慶北醫大雜誌 Vol.35 No.4

척추전방위증 환자에서 Interlaminar Resin Fixation과 Interspinous Wiring

최영근,황정현,성주경,황성규,함인석,박연묵,김승래 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.4

목적 : 척추전방전위증 환자에서 신경감압술의 시도와 함께 척추 병변분절의 고정을 이하여 골유합 고정술 대신 interspinous wiring과 interlaminar resin fixation을 시도했을 때 그 치료의 결과를 분석하고 관찰하는데 그 목적이 있다. 대상 및 방법 : 척추전방전위증 환자 남녀 6명을 대상으로 하여 척추병변 분절부위에 감압술의시도와 함께 골유합 고정술대신 interspinous wiring과 interiaminar resin fixation을 시도하여 1년 내지 2년동안 추적, 분석 관찰하였다. 결과 : 치료결과는 전례에서 술전에 있었던 신경학적 재증상과 소견은 현저히 호전되어 만족할만한 수술결과를 관찰할 수 있었으며 상당한 정복은 물론 더이상 전방전위의 진행도 없었다. 결론 : 척추전방전위증 환자에서 본 술법의 적용은 간편하고 용이하며, 척추 병변시술부위에 조작의 부담을 경감시킬 수 있고 Osteoporosis환자에서도 적용이 가능한 잇점과 고가장치를 사용치 않고도 병변분절의 고정이 가능한 경제성의 술법으로 사료되었다. Bilateral full foraminotomy for neural decompression and interlaminar resin fixation combined with interspinous wiring for stabilization in lumbar spondylolisthesis were performed without bone fusion. We observed postoperatively six cases of low grade of lumbar spondylolisthesis with neurological symptoms and signs. Preoperative neurological symptoms as lumbago, radiating pain along the legs and intermittent neurogenic claudication were relieved satisfactorily after surgery. But mild lumbar stiffness for the resin fixation, mild lumbago and tingling on the legs were developed on occasions without any disturbance of daily living life in all patients. There has been no any complication in surgical region of all patients with lumbar spondylolisthesis during postoperative follow-up period. This precedure of internal interiaminar resin fixation with interspinous wiring for spinal stability might be surgically simpler and safer than internal fusion fixation with metal instrumentation, and it might be economical surgery for lumbar spondylolisthesis as well.

간질 환자와 의료인이 인지하는 간질 환자를 위한 교육 요구도 비교조사

최미리,김연희,소연자,윤선무,이근숙,임상순,김금순,최스미 대한간호학회 2000 Journal of Korean Academy of Nursing Vol.30 No.6

Purpose : To determine whether there is a discrepancy between the medical professions perception of what patients should know and that of the patients themselves, we studied patients need to be informed about different aspects of epilepsy and compared findings with medical personnels perceptions of the issue. Methods : Our study population consisted of 39 patients with epilepsy from the inpatient epilepsy unit, and 51patients from the outpatients clinic of the S. University Hospital between July and November 1997. However, the patients who declined to participate or who were not able to understand the directions and content of the questionnaire were excluded. The medical personnel participated in this study were 56 residents or nurses who were working in either Neurology or Neuro surgery Units. The questionnaire consisted of 6 categories with a total of 79 questions. The responses were indicated on a 5point Likert scale with 5 indicating the highest need. The data were analyzed with descriptive statistics, students t-tests, and chi-square. Results : Of the 90 patients and 56 medical personnel studied, the need for lifestyle information such as smoking, drinking, sleep, driving, employment, and marriage was significantly higher from medical personnel than that of the patients(p=0.00). Regarding medical knowledge about epilepsy, the patients group had higher scores in the need for information on the structure of the brain (p=0.00), whereas medical personnel had higher scores on the symptoms of epilepsy. There was no correlation between the length of epilepsy and the need for information on every item on the questionnaire. The patients had higher rank regarding diet, although it was not significantly different from the medical personnel. Regarding antiepileptic drugs and what to do when there is an attack, medical personnel scored higher. The items on which the patients group scored higher than 4.5 were the possibility of inheritance, the factors that might reduce the number of attacks, the period of usage of AED, and the food they have to avoid or the food they have to take to reduce seizure attacks. Conclusions ; Our study indicates that the patients group requires higher educational need in the structure of the brain, diet, and surgical treatment, but less in lifestyles and what to do when there is an attack. The educational program for the patients with epilepsy should emphasize medical knowledge with regard to brain anatomy, what to eat and what to avoid, and details of surgical treatment.