Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

Song,Moon-Tae,Lee,Jeom-Ho,Lee,Sang-Bok,Cho,Youn-Sang,Ku,Ja-hwan,Seo,Kyoung-In,Choi,Seong-ho,Hwang,Heung-Goo 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

Evaluation Index System for Disaster Prevention Signs in Urban Shelters in China

Song,Chen,Zhang,Jingxing,Kim,Tae-Hwan 한국화재소방학회 2016 한국화재소방학회논문지 Vol.30 No.1

Reasonable disaster prevention signs play an important role in guiding evacuation. Through the field investigation on disaster prevention signs in shelters and surrounding areas in Beijing, some problems were found in the using of sign system. Based on the principle of integrated design, evacuation and rescue requirements, it is necesssary to make further study on aspects such as, design of function, systematic consideration, humanization design, as well as the internationalization using. This paper presents an evaluation index system for disaster prevention signs. Such a system is very important for strengthening the independent guiding function of sign systems and improving evacuation efficiency. An effective connection could be realized between the internal environment of buildings, evacuation routes, and emergencey shelters.

수정 Cam-clay 이론을 이용한 점토의 비배수 강도 예측에 관한 연구

이송 ( Lee¸ Song ),이규환 ( Lee¸ Kyu-hwan ),김태훈 ( Kim¸ Tae-hwoon ) 한국구조물진단유지관리공학회 2002 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.6 No.2

국내 지역에서 대부분의 자연 연약점토는 약간 또는 매우 과압밀된 상태를 갖고 있다. 이러한 연약한 지반에 제방을 건설하기 위해서는 지반의 지지력이 결정되어야 하며, 응력이력에 따른 비배수 전단강도는 단계하중재하시 재방의 단계성토고와 안정성을 분석함에 매우 중요하게 하다. 이러한 점에서 한계상태이론의 Cam-clay 이론은 정규압밀과 과압밀된 점토지반의 비배 수 전단강도를 예측함에 있어 합리적인 결과를 주며, 비등방조건에서 비배수 전단강도의 예측 은 실내삼축시험과 잘일치하고 있다. 따라서, 본 논문에서는 MCCM 이론의 적용성을 국내 저소성 실트질 점토를 블록 샘플링하여 실내 등방 및 비등방 시험을 실시하여 검증하였으며, 과거 여러 지역에서 연구된 결과들과 비교하여 재분석하였다.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

文章題解決과 讀解力과의 關係에 관한 연구

宋煥龍,申卿子,羅炳昭 春川敎育大學 1987 論文集 Vol.27 No.-

성인의 적응장애

송지영,장환일 한국의학사 1986 診斷과治療 Vol.6 No.5

혈액투석중인 만성신부전 환자에서 골대사 지표로써의 Osteocalcin치

송치운,이진홍,안미애,윤환중,윤상임,성기양,이강현,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Background : Serum osteocalcin is synthesized by osteoblast and has been shown to be sensitive indicator of bone turnover inpatients with various metabolic bone disease. In renal osteodystrophy, serum osteocalcin is elevated due to decreased renal clearance and elevated level of PTH. This study was done to evaluate the usefulness of serum osteocalcin as a marker of bone metabolism and the correlation with other biochemical markers of bone metabolism. Methods : We measured serum osteocalcin, calcium, phosphorus, ALP(alkaline phosphatase) and PTH(parathyroid hormone) in 37 patients with end stage renal disease on hemodialysis. Osteocalcin was determined by radioimmunoassay and PTH was determined by radioimmunometric assay. Results : 1) The mean level of serum osteocalcin in ESRD patients was 233.8± 218.2ng/ml which was significantly higher than that of controls(p<0.0001). 2) The mean level of serum PTH in ESRD patients was 40.5± 43.8pg/ml was significantly higher than that of controls(p<0.005). 3) There was a significant positive correlation between the level of serum PTH, ALP and the level of serum osteocalcin in ESRD patients. 4) By using multiple regression, PTH is most reliable factor that affect to elevated level of serum osteocalcin ( beta coefficient = 0.687, Sig T<0.05). Conclusion : Serum osteocalcin as a marker of bone metabolism in ESRD patients is more useful than other biochemical marker such as serum calcium, phosphorus, ALP and PTH is a most reliable factor that affect to elevated level of serum osteocalin.

긴장증의 임상적 특성에 대한 고찰

송지영,윤도준,변재영,장환일 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.3

26 cases(9 males and 17 females) with catatonia features on admission were analyzed in terms of its clinical characteristics. The results obtained were as follows : 1) In the diagnostic distribution, more than a half of the cases were schizophrenic disorders(57.5%) and the others were major depressions(26.9%) and schizophreniform disorders(15.4%). 2) The catatonia signs were nonspecific and evenly distributed among a variety of clinical diagnostic entities. But the signs of negativism, mutism, and posturing appeared frequently in all cases. 3) Acute onset was prevalent(65.4%), ECT was more frequently applied(61.5%) and treatment response was better(70.9%) in the catatonia group than non-catatonia. Although the definition of catatonia is not clear yet, above results support the opinions of previous studies that catatonia should be understood as a heterogenous symptom complex. And not only schizophrenia but also affective disorder are associated with catatonia features. This suggests that we have to re-evaluate the concept of Kahlbaum's old description.

全北地域 農村工業開發이 水質 環境에 미치는 影響에 關한 硏究

김환기,송호면,김진덕 全北大學校 附設 都市및環境硏究所 1995 都市 및 環境硏究 Vol.10 No.1

This study was performed on 27 agricultural industrial parks locating in Chonbuk Province for examining the influence of local environment with situation of location, actual condition of occupied plant with industrial classification, and relations of streams. The obtained results are as follows 1) An environmental impact assessment for agricultural industrial park needs for the selection of live-in plants. The method of regulation of total emission deciding the amount of wastewater discharge and its concentration according to the given condition in the water area of discharge needs to be introduced. 2) Because most of discharge streams from the agricultural industrial parks is small and their purposal water qulity belongs to Ⅰ∼Ⅱ grade, the worse possible of water qulity in the water area of discharge is great. 3) A common wastewater plant on agricultural industrial park applies a biochemical treatment method and the discharge water qulity appears to be contented the legal criteria, but it appears to be necessary the strengthen of treatment facilities and continual control because the water qulity of influx shows great difference from 14㎎/ℓ to 340㎎/ℓ according to each agricultural industrial park. 4) In case of considering the characteristic of wastewater on it and small business, the mixing treatment with urban sewage is desirable and the individual treatment facility of agricultural industrial park should need a common treatment as possible.

대조수사학과 상호작용적 텍스트 이론

김성환 동아대학교 인문과학대학 영어영문학과 1996 동아영어영문학 Vol.12 No.-

This study describes recent advances of the contrastive rhetorics and the interactive text theory in written text analysis The text written by native speakers was based on the culture - specific logical order.(Kaplan 1996) Thus contrastive rhetoric studies potentially have a great deal to offer the analysis of the cross-language written text and the writing/reading instruction in the area of ESL/EFL. Two major approaches-formalistic and interactive-were distinguished among the studies of contrastive rhetoric. The former emphasizes the description of the text-type and formal rhetoric pattern, while the latter regards the text as the interactive and dynamic communications, including the contextual factors. Now the interactive approaches toward text are increasingly promoted in the study of the constrastive rhetoric. One of the most promising approaches to text analysis is the one taken by Beaugrande & Dressler(1981), Which draws heavily on a view of text as communicative interaction. The central notion of their work is that text in defined as a communicative occurrence which meet these seven features of textuality. Also these seven features were divided the text-centered features and the user-centered features. The discussions of the textual features suggest directions toward which future theories of text directly applicable to contrastive rhetoric may develop The implications of the above discussions for the conduct of the contrastive rhetoric study and the teaching of ESL/EFL writing and reading are examined.