位相的 解析에 依한 韓國住居의 原型 硏究

宋容浩,李鍾國,任亮彬 충남대학교 공업교육연구소 1984 論文集 Vol.7 No.2

In this paper, it is aimed to study the architype of Korean houses with topological analysis. So, first, the meaning & rationality of topological analysis on houses is discussed. Second, the traditional houses devided into Minka & Banka(Korean houses name of low & high society's) are analyzed. Third, the modem houses built since 1970 are analyzed, too. And last, hoses of both traditional ones and modern ones are interpretated comparatively. Conclusionally, the fact that architype of Korean houses comes from Minka is verified.

알타리무우김치 熟成過程中의 遊離아미노酸의 組成變化

文淑任,趙鏞桂,方良仙 東亞大學校附設基礎科學硏究所 1985 基礎科學硏究論文集 Vol.2 No.1

This study was carried out to compare the charge of free amino acid content in the radish Kimchi added with anchovy pickle sauce(sample A), with that added with 15% NaCl solution(sample B) during 30 days fermentation. The results were summarized as follows; 1. During the fermentation, the pH of both sample A and B showed the highest values of 7.3 and 7.1, respectively, and then both continually decreased to the lowest values of 4.2 at the 30th day. 2. The salininity in the juice of sample A was higher than that of sample B in all the steps during fermentation, accompanied with a slight decrease of salinity with time elapsed in both cases. 3. The free amino type nitrogen content of sample A was nearly doubled in the final step than in the initial one, while that of sample B showed no significant changes in all the processing period. 4. The free amino acids detected in both samples were aspartic acid, threonine, serine, proline, glutamic,acid,glycine,alanine, tyrosine, cysteien, lysine, methionine, isoleucine, leucine, phenylalanine, Υ-amino butyric acid(?), ornithine, histidine and arginine. 5. The amount of proline and/or arginine was the highest in all the free amino acid content, and tasty compounds derived from amino acids from radish Kimchi seemed to be ascribed to glutamic acid, alanine, isoleucine,leucine and phenylalanine. The amino acids (such as alanine, valine, phenylalanine and lysine.) of anchovy pickle sauce used, seemed to cause Kimchi A to be more tasteful than B.

Gerbera(Gerbera hybrida Hort)의 형태적 특징과 유전적 유연관계 분석

김현애,임현희,양원진,이재헌,이병영,이용문,권오창 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was conducted to analyze the morphological characteristics and genetic relationships among 24 species of Gerbera. Most of flower colors were pink. The numerical order of flower color was pink, orange, red, double-colored, and milk-white. Majority of flower types were single or semidouble flowers. A few species were double flowers. Flower diameters were from 7cm to 12cm, showed significant differences compared to other characteristics. Flower stalks were ranged from 55cm to 65cm. Only one species was the shortest as 55cm. The others were similar size as about 65cm. Main annual production yields were between 190 and 400 blossoms. Fifty seven reproducible polymorphic bands from eighty primers were used for analyses of genetic similarity. The genetic similarity of 24 collected Gerberas was largely classified into five groups. The average similarity coefficient was 0.72 ranged from 0.50 to 0.90. The highest similarity coefficient was shown between 'Sardana' with red/white flower color and double flower type, and 'Tamara' with orange flower color and double flower type as 0.90.

Mito-Tempo Can Protect Against the Allergic Airway Inflammation through Reducing Mitochondrial ROS Generation

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.

The Relationship between Mitochondrial ROS and ER Stress in Allergic Airway Diseases

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Mi Ran Kang ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Hee Jung Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Mitochondria and the Nox family of NADPH oxidase are the two major sources of reactive oxygen species (ROS) that are induced by external stimuli, and the mitochondria respiratory chain is considered as an important site of ROS production within most cells. Recent evidence has demonstrated that various biological stimuli increase accumulation of unfolded or mis-folded proteins in ER lumen, which is referred to as "ER stress". Moreover, these various pathologic stimuli have been reported to provoke oxidative stress as well as ER stress. In this study, we used the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice) for elucidation of the relationship between mitochondrial ROS and ER stress in bronchial asthma. The OVALPS-OVA mice showed that the expression of ER stress markers and the protein levels of un-folded-protein response (UPR)-related marker in lung tissues were significantly increased after OVA challenge. In addition, we visualized the localization of mitochondrial ROS in BAL cells isolated from OVALPS-OVA mice using confocal microscopy; the significant increase in mitochondrial ROS in BAL cells was observed after OVA challenge. Our results also showed that Necrox-5 or 4-PBA significantly reduced the increases in ER stress, mitochondrial ROS, inflammatory cytokines, airway in-flammation, and bronchial hyperresponsiveness. These findings suggest that mitochondrial ROS and ER stress plays an im-portant role in the induction and maintaining allergic airway diseases synergistically.

플륨 모니터링에 의한 SM45C 레이저 용접특성 평가

유영태,김재열,노경보,양동조,오용석,임기건,김지환 한국공작기계학회 2003 한국생산제조학회지 Vol.12 No.6

With the increased use of lasers in industrial welding applications, techniques for monitoring and controlling these processes become increasingly important. It is very important that we understand the dynamic behaviors of the laser induced plume in welding because the laser induced plume has considerable effects on welding efficiency and the quality of materials. As the plume fluctuation was associated with keyhole instability, unstable vapor plume indicated the process was unstable and would result in poor welds. An Infrared Themal-vision Camera can be utilized compensate for incurracies encountered in real-time monitoring during laser welding. We have results that instabilities of plume are closely dated with hot cracking and defect of laser welding.

이질아메바에 의한 인체 대장상피세포주 HT-29에서의 interleukin-8 유전자의 발현

김정목,정현채,임경일,조양자,김정룡 INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

이질아메바에 의한 장염 환자의 조직 또는 이질아메바를 실험적으로 감염시킨 동물의 조직 검사에서 호중구의 침윤이 특징적으로 관찰된다. 그러나 이와같은 호중구의 침윤을 설명할 수 있는 기전에 대한 연구는 매우 미흡하다. 따라서 본 연구자들은 아메바 감염 초기에 인체 대장상피세포에서 interleukin-8(IL-8)이 유도되어 호중구 침윤과 같은 염증반응이 유발될 것이라는 가설을 설정하였다. 이를 위하여 인체 대장상피세포주인 HT-29에 이질아메바 영양형을 실험적으로 노출시킨 뒤 발현되는 IL-8 mRNA를 역전사 중합효소법(reverse transcriptional polymerase chain reaction, RT-PCR)으로 검사함과 동시에 발현된 IL-8 mRNA를 인공적으로 합성시킨 표준 RNA와 RT-PCR법을 이용하여 정량하였다. 실험 결과 이질아메바 영양형에 노출된 30분 후 부터 IL-8 mRNA가 발현되기 시작하였다. 그리고 그 발현 분자수는 노출 시간의 증가에 따라 계속 증가하여 3시간 대에는 3.1×10(7) molecules/㎍ total RNA를 나타내었다. 동시에 IL-8 mRNA의 발현은 노출시킨 이질아메바 영양형의 수에 비례하였다. 즉, HT-29/아메바 영양형의 비율이 10:1인 경우 IL-8 mRNA의 발현 분자수는 1.2×10(7) molecules/㎍ total RNA로 나타났다. 이와같은 IL-8 mRNA의 발현은 IL-8 단백질 분비로 이어짐을 ELISA 검사로 확인할 수 있었다. 한편 이질아메바 파쇄액(lysate)도 대장상피세포주인 Caco-2에서 IL-8 mRNA발현을 유도하였다. 결론적으로 본 실험은 이질아메바 감염 초기에 대장상피세포로 부터 IL-8이 발현되며 이에 의하여 염증반응이 촉발될 가능성이 있음을 시사해 준다. The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interlukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoities for 30 minutes. 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1×10(7) molecules/㎍ of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results suggest that acute inflammatory reaction by E. hisstolytica may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.

Two cases of familial cerebral cavernous malformation caused by mutations in the CCM1 gene

Im-Yong Yang,Mi-Sun Yum,김은희,Hae-Won Choi,Han-Wook Yoo,Tae Sung Ko 대한소아청소년과학회 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.6

Cerebral cavernous malformation (CCM) is a vascular malformation characterized by abnormally enlarged capillary cavities without any intervening neural tissue. We report 2 cases of familial CCMs diagnosed with the CCM1 mutation by using a genetic assay. A 5-year-old boy presented with headache, vomiting, and seizure-like movements. Brain magnetic resonance imaging (MRI) revealed multiple CCM lesions in the cerebral hemispheres. Subsequent mutation analysis of his father and other family members revealed c.940_943 del (p.Val314 Asn315delinsThrfsX3) mutations of the CCM1 gene. A 10-month-old boy who presented with seizure-like movements was reported to have had no perinatal event. His aunt was diagnosed with cerebral angioma. Brain and spine MRI revealed multiple angiomas in the cerebral hemisphere and thoracic spinal cord. Mutation analysis of his father was normal, although that of the patient and his mother revealed c.535C>T (p.Arg179X) mutations of the CCM1 gene. Based on these studies, we suggest that when a child with a familial history of CCMs exhibits neurological symptoms, the physician should suspect familial CCMs and consider brain imaging or a genetic assay.

Composition Analysis and Inhibitory Effect of <i> Sterculia lychnophora</i> against Biofilm Formation by <i> Streptococcus mutans</i>

Yang, Yang,Park, Bok-Im,Hwang, Eun-Hee,You, Yong-Ouk Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Pangdahai is a traditional Chinese drug, specifically described in the Chinese Pharmacopoeia as the seeds of<I> Sterculia lychnophora</I> Hance. Here, we separated<I> S. lychnophora</I> husk and kernel, analyzed the nutrient contents, and investigated the inhibitory effects of<I> S. lychnophora</I> ethanol extracts on cariogenic properties of<I> Streptococcus mutans</I>, important bacteria in dental caries and plaque formation. Ethanol extracts of<I> S. lychnophora</I> showed dose-dependent antibacterial activity against<I> S. mutans</I> with significant inhibition at concentrations higher than 0.01 mg/mL compared with the control group (<I>p</I> < 0.05). Furthermore, biofilm formation was decreased by<I> S. lychnophora</I> at concentrations > 0.03 mg/mL, while bacterial viability was decreased dose-dependently at high concentrations (0.04, 0.08, 0.16, and 0.32 mg/mL). Preliminary phytochemical analysis of the ethanol extract revealed a strong presence of alkaloid, phenolics, glycosides, and peptides while the presence of steroids, terpenoids, flavonoids, and organic acids was low. The<I> S. lychnophora</I> husk had higher moisture and ash content than the kernel, while the protein and fat content of the husk were lower (<I>p</I> < 0.05) than those of the kernel. These results indicate that<I> S. lychnophora</I> may have antibacterial effects against<I> S. mutans</I>, which are likely related to the alkaloid, phenolics, glycosides, and peptides, the major components of<I> S. lychnophora</I>. </P>

Role of Hydroxyl Radicals and Lipid Peroxidation in Cisplatin-induced Acute Renal Failure in Rabbits

Yang, Yong Suk,Kwak, Im Soo,Nah, Ha Yun,Kim, Yong Keun 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : Cisplatin은 여러 종류의 고형성 암의 치료제로 널리 사용되는 약물이지만, 동시에 심각한 신장독성을 나타내는 것으로 잘 알려져 있다. 게다가 cisplatin이 어떤 기전으로 신장독성을 나타내는지도 명확히 밝혀져 있지 않다. 따라서 본 연구에서는 hydroxyl radical들과 지질의 과산화가 cisplatin에 의한 급성신부전에 중요한 역할을 하는지를 조사하였다. 방 법 : 토끼를 실험동물로 사용하여 cisplatin 5mg/kg 용량을 복강내 주사하여 급성신부전을 유발시키고 cisplatin을 주사한 48시간 동안 신장기능의 변화를 조사하였다. 결 과 : Cisplatin 주사 후에 혈청내 creatinine농도증가와 사구체여과율의 감소가 나타났으며, Na^(+), 포도당 및 무기인산의 배설분율의 증가가 나타났다. Cisplatin처치는 신피질절편에서 PAH 축적과 microsomalfraction에서 Na^(+)-K^(+) ATPase 활성을 감소시켰다. Cisplatin을 주사한 동물의 신장조직에서는 지질의 과산화가 증가하였다. Cisplatin을 주사하기 전에 항산화제인 N,N-diphenyl-p-phenylenediamine(DPPD), 철착염제인 deferoxamine, hydroxyl radical scavenger들인 dimethylthiourea와 sodium benzoate을 처치한 결과 cisplatin에 의한 급성신부전이 유의하게 방지되었으며, 지질의 과산화도 억제되었다. 시험관내에서 정상 신장의 피질절편을 cisplatin에 노출시켰을 때 lactate dehydrogenase(LDH) 유출과 지질의 과산화가 증가하였으며, 이러한 변화는 DPPD와 deferoxamine에 의해 방지되었다. 그러나 hydroxyl radical scavenger들인 dimethylthiourea와 thiourea는 cisplatin에 의한 지질의 과산화는 방지하였으나 LDH 유출에는 영향을 미치지 못하였다. 결 론 : 이러한 연구 결과는 cisplatin이 급성신부전을 유발하는데는 hydroxyl radical의 생성과 이로 인한 지질의 과산화가 중요한 역할을 한다는 것을 가리킨다. 그러나 hydroxyl radical들은 cisplatin에 의한 신장독성의 초기 단계에는 관여하지 않는 것으로 생각된다. Purpose : This study was undertaken to determine whether lipid peroxidation induced by hydroxyl radicals play a critical role in cisplatin(cis-diamminedichloroplatinum II)-induced acute renal failure. Methods : Animals received cisplatin at a single i.p. dose of 5 mg/kg, and changes in renal function were measured at 48 hr after cisplatin injection. Results : Cisplatin caused an increase in serum creatinine level, which was accompanied by reduction in GFR. The fractional excretion of Na^(+), glucose, and inorganic phosphate was increased in animals treated with cisplatin alone. Cisplatin treatment in vivo inhibited PAH uptake by renal cortical slices and Na^(+)-K^(+)-ATPase activity in microsomal fraction. Lipid peroxidation was increased in cisplatin-treated kidneys. When animals received the antioxidant N,N'-diphenyl-p-phenylenediamine(DPPD), the iron chelator deferoxamine, and hydroxyl radical scavengers dimethylthiourea and sodium benzoate before cisplatin injection, alterations in renal function and lipid peroxidation induced by cisplatin were significantly prevented. Exposure of renal cortical slices to cisplatin in vitro caused an increase in LDH release and lipid peroxidation, which were completely prevented by DPPD and deferoxamine. By contrast, hydroxyl radical scavengers(dimethylthiourea and thiourea) did not prevent cisplatin-induced LDH release despite they inhibited cisplatin-induced lipid peroxidation. Conclusion : These results suggest that the lipid peroxidation resulting from generation of hydroxyl radicals may play a role in cisplatin-induced acute renal failure. In addition, the protective effects of hydroxyl radical scavengers in vivo studies are different from the results obtained from in vitro studies using renal cortical slices. (Korean J Nep h rol 2 00 2;2 1( 2):2 13 -22 1)