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Jae-Uoong Shim,Joon Haeng Rhee,Young-Il Koh 대한천식알레르기학회 2012 Allergy, Asthma & Immunology Research Vol.4 No.5
Purpose: Invariant natural killer T (iNKT) cells may play an important role in the pathogenesis of asthma in mice and humans. Thus, an agent that modulates the function of iNKT cells may have therapeutic potential to control asthma. We hypothesized that lipopolysaccharide (LPS)-, flagellin-, or CpG-induced changes in the cytokine milieu may modify and even inhibit the function of airway iNKT cells in asthma. Methods: Because increased α-galactosylceramide (GalCer)-induced airway hyperreactivity (AHR) reflects the presence of airway iNKT cells, α-GalCer-induced AHR, as well as inflammatory cells and cytokines in bronchoalveolar lavage (BAL) fluid, were determined 24 hours after in vivo treatment with LPS, flagellin, or CpG in naïve BALB/c mice. Intracellular IL-4 and IFN-γ were measured in spleen iNKT cells after in vitro treatment with LPS, flagellin, or CpG. A role for IL-12 following the treatments was determined. Results: Intranasal administration of LPS, flagellin, or CpG reduced development of α-GalCer-induced AHR, eosinophilic airway inflammation, and Th1 and Th2 cytokine responses in BAL fluid, while producing IL-12 in BAL fluid. Intraperitoneal administration of IL-12 mAb blocked the suppressive effect of LPS, flagellin, or CpG. In vitro treatment with LPS, flagellin, or CpG reduced production of IL-4 and IFN-γ from α-GalCer-stimulated spleen iNKT cells; these effects were ameliorated by addition of anti-IL-12 mAb. Conclusions: TLR4, 5,and 9 agonists may suppress the function of airway and spleen iNKT cells via IL-12-dependent mechanisms. Anergy of iNKT cells by IL-12 might play a role in suppression by these TLR agonists.
한국인 원발성 사구체신염에서의 IL-1β, IL-1Ra, TNF-α 유전자 다형성에 관한 연구
박미나 ( Mi Na Park ),강양일 ( Yang Il Kang ),이상열 ( Sang Youl Rhee ),정래익 ( Lae Ik Jeong ),나승연 ( Seung Yeon Na ),정경환 ( Kyung Hwan Jeong ),이상호 ( Sang Ho Lee ),이태원 ( Tae Won LEE ),임천규 ( Chun Gyoo Ihm ) 대한신장학회 2006 Kidney Research and Clinical Practice Vol.25 No.2
탈미네랄화 골분(DBP)이 PLGA 지지체의 염증반응을 완화시킨다
최방실 ( Bang Sil Choi ),김순희 ( Soon Hee Kim ),윤선중 ( Sun Jung Yun ),하현정 ( Hyun Jung Ha ),김문석 ( Moon Suk Kim ),양영일 ( Young Il Yang ),손영숙 ( Young Sook Son ),강길선 ( Gil Son Khang ),이종문 ( John M. Rhee ),이해방 ( 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3
We developed the demineralized bone particle(DBP) impregnated poly(lactide-co-glycolide)(PLGA) scaffolds(PLGA/DBP) to investigate the effect of adhesion, growth, viability and inflammatory reaction of the cells. PLGA/DBP scaffolds were prepared by solvent casting/salt leaching method and were characterized by porosimeter, and scanning electron microscopy. NIH/3T3 fibroblast cells were cultured in the PLGA/DBP scaffolds as well as film and MTT assay was used to assess the viability of cells. Also, human promyelocytic leukemia cells(HL-60) were cultured in the PLGA/DBP scaffolds. We observed IL-1ß? and TNF-α expression of HL-60 cells seeded in PLGA/DBP scaffold by RT-PCR. NIH/3T3 fibroblast cell seeded on PLGA/DBP film were more adhere and spread with increasing DBP content due to increasing hydrophilicity and bioactivity. The fluorescence intensity of the band of TNF-α and IL-1ß? gene was decreased with increasing the concentration of DBP. It seems that the DBP affected on the improvement of physicochemical properties of PLGA such as biocompatibility, wettability and inflammatory response.
Yoon, Sun Jung,Kim, Soon Hee,Ha, Hyun Jung,Ko, Youn Kyung,So, Jung Won,Kim, Moon Suk,Yang, Young Il,Khang, Gilson,Rhee, John M.,Lee, Hai Bang Mary Ann Liebert 2008 Tissue engineering. Part A Vol.14 No.4
<P>Poly(lactide-co-glycolic acid) (PLGA) has been widely applied to tissue engineering as a good biocompatible material because of its biodegradability and nontoxic metabolites, but how the inflammatory reaction of PLGA on the surrounding tissue in vivo is reduced has not been discussed sufficiently. We hypothesized that the cells neighboring the PLGA implant might have an inflammatory response that could be reduced by impregnating demineralized bone particles (DBPs) into the PLGA. We manufactured five different ratios of DBP/PLGA hybrid materials, with each material containing 0, 10, 20, 40, and 80 wt% of DBPs of PLGA. For biocompatibility test, NIH/3T3 mouse fibroblasts were cultured on the DBP/PLGA scaffold for 3 days. The inflammatory potential of PLGA was evaluated using messenger ribonucleic acid expression of tumor necrosis factor alpha (TNF-alpha) and interleukin 1-beta (IL-1beta) on a human acute promyelocytic leukemic cell (HL-60). The in vivo response of DBP/PLGA film was compared with that of PLGA film implanted subcutaneously; the local inflammatory response was observed according to histology. The DBP/PLGA scaffold had no adverse effect on NIH/3T3 initial cell attachment and did not affect cell viability. DBP/PLGA films, especially PLGA films containing 80% DBP, elicited a significantly lower expression of IL-1beta and TNF-alpha from HL-60 cells than PLGA film alone. In vivo, DBP/PLGA film demonstrated a more favorable tissue response profile than PLGA film, with significantly less inflammation and fibrous capsule formation as below only 20% of DBP in PLGA film during implantation. This study shows that application of DBPs reduces the fibrous tissue encapsulation and foreign body giant cell response that commonly occurs at the interface of PLGA.</P>
李春根,金圭植,南日祐,河正洙 中央醫學社 1967 中央醫學 Vol.12 No.1
We have studied on clinical and statistical studies of periodontal cysts developed from 46 patients. The results were obtained as follows, 1) Periodontal cysts are more frequent in females (52.17%) than that in males (47.83%). 2) Predominant ages are third decade (34.78 % ), fifth decade (23. 91 % ), second decade and fourth decade (each other 17.39%) and sixth decade (6.52 %) in the orders. 3) In the etiological teeth of periodontal cysts, the upper central and lateral incisors, lower first and second molars are prevalent. 4) periodontal cysts are more frequent in maxilla (70 %) than that in mandible (30%). The predominant region of periodontal cysts are anterior region of maxilla and molar region of mandible. 5) The 46 periodontal cysts have been treated by means of direct enucleation technique of cyst walls in 43 cases and apicoectomy in 3 cases.