West Nile Virus detection and blood meal identification from captured mosquitoes in urban parks in Korea

Hyunwoo Kim,Sung-Chan Yang,Byung-Eon Noh,Eun Jung Lee,Jin-Hwan Jeon,Se-Jin jeong,Shin-Hyeong Cho,Wook-Gyo Lee 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

West Nile Virus (WNV) is transmitted by infected mosquitoes. Vector mosquitoes usually acquire these pathogens fromfeeding on an infected host, and transmit the pathogens to a naive host during feeding events. To understand the virustransmission dynamics and to survey WNV throughout country, the present study has been conducted. We collected mosquitoesat urban parks in Seongnam, Wonju, Gunsan, Daegu, and Tongyeong using CDC light trap with Dry ice from April toSeptemper in 2017 (mosquito collecting is on going). Among collected mosquitoes, blood-fed mosquitoes were conductedblood meal identification assay and the other mosquitoes were subjected to virus detection using real-time PCR method.A total of 2,290 mosquitoes representing 6 genera and 15 species were collected. The most dominant species was Culexpipiens complex (42.1%) followed by Aedes albopictus (15.1%), Ae. vexans nipponii (14.6%), Ochlerotatus koreicus (9.8%),Cx. orientalis (6.5%), and Armigeres subalbatus (4.4%). The blood meal source were of mammal (93.3%), and birds (6.7%).So far, no WNV has been detected in any mosquitoes.

Detection of Mycobacterium leprae by Real-time PCR Targeting Mycobacterium leprae-Specific Repetitive Element Sequence

Hyunwoo Jin,Hye-Young Wang,Jong-Pill Kim,Sang-Nae Cho,Hyeyoung Lee 대한의생명과학회 2010 Biomedical Science Letters Vol.16 No.2

Mycobacterium leprae detection is difficult even with molecular biological techniques due to the low sensitivity of current methodologies. In this report, real-time PCR targeting the M. leprae-specific repetitive element (RLEP) sequence was developed as a new diagnostic tool and evaluated using clinical specimens. For this, M. leprae DNAs were extracted from skin biopsy specimens from 80 patients and analyzed by real-time PCR using TaqMan probe. Then, the detection efficiency of the real-time PCR was compared with that of standard PCR. In brief, the rate of positive detection by the standard PCR and real-time PCR was 32.50% and 66.25%, respectively. The results seemed to clearly show that the TaqMan real-time PCR developed in this study may be a useful tool for sensitive detection of M. leprae from clinical specimens.

High mobility, large linear magnetoresistance, and quantum transport phenomena in Bi2Te3 films grown by metallo-organic chemical vapor deposition (MOCVD).

Jin, Hyunwoo,Kim, Kwang-Chon,Seo, Juhee,Kim, Seong Keun,Cheong, Byung-Ki,Kim, Jin-Sang,Lee, Suyoun RSC Pub 2015 Nanoscale Vol.7 No.41

<P>We investigated the magnetotransport properties of Bi2Te3 films grown on GaAs (001) substrate by a cost-effective metallo-organic chemical vapor deposition (MOCVD). We observed the remarkably high carrier mobility and the giant linear magnetoresistance (carrier mobility ??? 22???000 cm(2) V(-1) s(-1), magnetoresistance ??? 750% at 1.8 K and 9 T for a 100 nm thick film) that depends on the film thickness. In addition, the Shubnikov-de Haas oscillation was observed, from which the effective mass was calculated to be consistent with the known value. From the thickness dependence of the Shubnikov-de Haas oscillation, it was found that a two dimensional electron gas with the conventional electron nature coexists with the topological Dirac fermion states and dominates the carrier transport in the Bi2Te3 film with thickness higher than 300 nm. These results are attributed to the intrinsic nature of Bi2Te3 in the high-mobility transport regime obtained by a deliberate choice of the substrate and the growth conditions.</P>

RT-PCR Targeting rpoB mRNA for Drug Susceptibility Test of Mycobacterium tuberculosis in Liquid Culture

Hyunwoo Jin 대한의생명과학회 2016 Biomedical Science Letters Vol.22 No.4

The problems of tuberculosis and its drug resistance are very severe. Therefore, rapid and accurate drug susceptibility assay is required. Recently, there has been an increased understanding of the genetic mechanism of Mycobacterium tuberculosis (MTB) drug resistance as well as advancement of molecular technologies. While many gene mutations correlate well with drug resistance, many genes do not show a strong correlation with drug resistance. For this reason, the current study assessed the utility of rpoB mRNA as a target to detect live mycobacteria. In this study, RT-PCR targeting of rpoB mRNA in BCG treated with rifampin was performed. Conventional RT-PCR and real-time PCR targeting rpoB mRNA as well as 85B mRNA was performed to determine whether these two methods could distinguish between viable and non-viable MTB. The levels of rpoB and 85B mRNA detected by RT- PCR were compared in parallel with colony forming unit counts of BCG that were treated with rifampin for different periods of time. The data suggests that that even though both mRNA levels of rpoB and 85B decreased gradually when rifampin-treatment increased, the rpoB mRNA seemed to represent live bacteria better than 85B mRNA. This study clearly indicates that RT-PCR is a good method to monitor viable cell counts in the liquid culture treated with the anti-tuberculosis drug.

Cell Proliferation and Antioxidative Effects of Ultrasonic Coffee Extracts

Hyunwoo Jin 대한의생명과학회 2017 Biomedical Science Letters Vol.23 No.4

Recently, coffee is the most popular beverage for modern people. A great number of substances are found in coffee beans and have been studied for many years such as aliphatic and aromatic compounds. However, studies on the physiological activity of coffee extracts are insufficient. This study was performed to determine the contents of caffeine and chlorogenic acid in coffee extracts according to the solvent and to investigate the physiological activity of coffee extracts. Coffee extracts were extracted by ultrasonication method with various types of solvents including distilled water, ethanol, and other organic solvents under 50℃ and 80℃. The contents of caffeine and chlorogenic acid in coffee extracts were determined by Liquid Chromatography-Mass Spectrometry (LC-MS). Also, cytotoxic and antioxidative effects of coffee extracts were evaluated with MTT and DPPH assays to analyze the physiological activity. As a result, it was confirmed that caffeine and chlorogenic acid contents were extracted in distilled water with the highest rate. Antioxidative activity was observed below 10-fold dilute of coffee extracts, however cytotoxicity was not observed. In conclusion, distilled water was the best solvent for extracting caffeine and chlorogenic acids from coffee bean with ultrasonication and these coffee extracts are less cytotoxic in human skin cell lines and have antioxidant effect.

Evaluation of a PCR-Reverse Blot Hybridization Assay to Identify Six Dermatophytes Predominant in the Republic of Korea

Hyunwoo Jin,Hyunjung Kim,Sunghyun Kim,Yeonim Choi,Hyeeun Bang,Sangjung Park,Hyeyoung Wang,Jang-Ho Lee,In Ho Jang,Young-Kwon Kim,Hyeyoung Lee 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.3

Accurate and rapid diagnosis of dermatophytosis, a disease whose prevalence has been steadily increased, is important for successful treatment. Current laboratory methods for diagnosing dermatophytosis rely on KOH mount and fungal culture method. However, these methods have low sensitivity and are time-consuming (2~4 weeks to diagnosis). In our previous study, a rapid molecular diagnostic assay (PCR-reverse blot hybridization assay, REBA) was developed to identify the following 6 main species of dermatophytes: Trichophyton rubrum, T. mentagrophytes, T. tonsurans, Microsporum canis, M. gypseum, and Epidermophyton floccosum. However, the REBA required more evaluation to validate its use in clinical examinations. The aim of the present study was to evaluate and validate the ability of the PCR-REBA to successfully identify dermatophytes in clinical isolates from dermatophytosis patients. Both conventional identification methods and the PCR-REBA were used to assess the presence of species of dermatophytes in 148 clinical isolates. The results of the two approaches were compared, and discrepancies between the two approaches were resolved by fungal ITS1 sequence analysis. T. rubrum was the most prevalent dermatophyte identified by conventional identification methods (118/148, 79.7%) and the PCR-REBA (131/148, 88.4%). The overall rate of consistency between conventional identification methods and the PCR-REBA was 79.0% (117/148 samples). Fungal ITS1 sequence analysis showed that PCR-REBA results were correct for 93.5% (29/31) of the discrepant samples. The PCR-REBA is rapid, sensitive, and highly specific compared with conventional identification methods. Thus, the PCR-REBA is a potentially useful tool for identifying dermatophytes in clinical settings.

발열체가 적용된 터널 라이닝 내부 및 배면의 온도변화 분석

진현우(Hyunwoo Jin),김태식(Teasik Kim),황영철(Youngcheol Hwang) 한국지반환경공학회 2017 한국지반환경공학회논문집 Vol.18 No.1

한랭지역에 건설된 터널 라이닝은 터널주변 지하수의 결빙 등으로 인한 균열, 누수 및 백태 등이 빈번하게 발생하게 된다. 이러한 터널의 동결피해는 동절기 외기온도에 의해 터널내부 라이닝 및 배면의 지반온도의 하강으로 발생하게 되므로 시공 시 단열재를 설치하여 동결로 인한 피해를 저감하거나 기 시공된 터널에서는 단열재를 설치하거나 발열체 등을 설치하여 동결로 인한 피해를 저감할 수 있다. 본 연구에서는 대기온도의 변화에 의한 라이닝 배면의 지반온도 변화와 발열체가 설치되었을 경우 라이닝 및 배면 지반의 온도변화를 분석함으로써 동결피해 저감을 위한 대응방안 시 나타나는 효과를 분석하였다. The damage of the tunnel lining on the cold regions can be represented by cracks and leaks caused by freezing of ground water. However, domestically, the relevant construction guidelines are not provided so far. Thus, in this research, the mechanical behavior and thermal conductivity of designated tunnel area are measured using instrumentation system installed in the lining concrete inside tunnels in order to analysis their behavior with regard to temperature variations. Previous research mainly focused on the effect of temperature on the tunnel lining based on the air and initial ground temperature at urban regions. Thus, this study analyzes effects of air temperature and initial ground temperature of designated tunnel area at the cold regions. The temperature of the groundwater at the backfill of the tunnel lining are analyzed to evaluate the heating element. Numerical analyses are performed to evaluate the heating element with regard to the various initial ground temperatures.

한국형 인공월면토 생산 시스템 효율성 및 Fe(0) 모사를 위한 수소 환원반응에 관한 실험적 평가

진현우(Jin, Hyunwoo),김영재(Kim, Young-Jae),류병현(Ryu, Byung Hyun),이장근(Lee, Jangguen) 한국지반공학회 2020 한국지반공학회논문집 Vol.36 No.8

마이크로파 소결 기반 한국형 인공월면토(KLS-1) 블록 제작 및 평가

진현우(Hyunwoo Jin),김영재(Young-Jae Kim),이장근(Jangguen Lee),신휴성(Hyu-Soung Shin) 한국항공우주학회 2023 한국항공우주학회 학술발표회 논문집 Vol.2023 No.11

한랭지역(강원권)에서의 도로터널 라이닝부 피해 현황 연구

진현우(Hyunwoo Jin),황영철(Youngcheol Hwang) 한국지반환경공학회 2017 한국지반환경공학회논문집 Vol.18 No.1

국내 한랭지역(강원권)의 경우 낮은 연평균 기온에 의한 도로터널 라이닝 내구성에 문제가 발생하게 된다. 이에 따라 라이닝에 균열, 망상균열, 박락, 박리, 누수 등의 문제가 발생하며, 해마다 경제적 손실 및 인명피해가 증가하고 있는 실정이다. 하지만 국내에서는 기온에 따른 도로터널 라이닝의 피해에 대한 연구는 미비한 상태이다. 따라서, 본 논문에서는 한랭지(강원권) 터널 70개를 조사함으로써 현재 한랭지역의 피해현황을 분석하였고, 같은 한랭지역 중 비교적 따뜻한 기온을 가지는 강릉 지역과 기온이 낮은 홍천 지역의 터널 라이닝 피해를 비교함으로써 기온이 도로터널 라이닝에 미치는 영향을 분석하였다. Due to low annual average temperature, road tunnel lining in domestic cold region (Gangwon province) experiences durability problems. The financial and human damage due to cracks, breakout, exfoliation and water leakage increases every year. However, domestic research on effect of temperature on road tunnel lining damage is insufficient. Thus, this research has investigated 70 tunnels located in cold region (Gangwon-do) to analyze damage status. Furthermore, by contrasting damage on tunnels in relatively warm Gangneung area with those in relatively cold Hongcheon area, the effect of temperature on road tunnel lining damage was analyzed.