PGE_2와 DBcAMP가 골조직 세포의 생리적 활성에 미치는 영향

이준기,채한정,강장숙,김형룡 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.2

One of the primary functions for which bones have evolved is to act as structural support. To achieve this goal, bones are remodeling throughout life so that their structure remains optimal for the prevailing mechanical environment. Bone remodeling consists of an initial phase of osteoclastic bone resorption followed by a bone formation period. Prostaglandins are potent regulators of bone formation and bone resorption that can have both stimulatory and inhibitory effects. Elevation of intracellular cAMP is an important intracellular signaling mechanism involved in the regulation of the expression of many proteins. In this study, the author examined whether PGE_2 or DBcAMP affected osteoblastic activation or osteoclastic differentiation in mouse bone marrow cells and osteosarcoma ROS 17/2.8 cells. The effect of PGE_2 and DBcAMP on the cell proliferation was measured by the incorporation of [^3H]-thymidine into DNA. From the result, PGE_2( 0.5-1 ㎍/ml ) and DBcAMP(0.1-0.5 mM) inhibited the [^3H]-thymidine incorporation into DNA. The effect of PGE_2 and DBcAMP on the induction of alkaline phosphatase(ALP) was investigated in ROS 17/2.8 cells cultured in medium containing 0.4% fetal bovine serum. PGE_2 and DBcAMP stimulated ALP activity in the cells. PGE_2 also increased the intracellular cAMPs contents with a maximal effect at 0.5 ㎍/ml. ROS 17/2.8 cells released nitric oxide upon stimulation of PGE2 or DBcAMP with interferon-γ. PGE_2 and DBcAMP increase the phosphorylation level of CREB(cAMP response element binding protein) without any change on the amount of CREB protein. Also, PGE_2 (10^-6 M) and DBcAMP (10^-4 M) significantly increase the generation of osteoclast-like cell in mouse bone marrow cell culture system. In conclusion, the results of this study suggested that PGE_2 and cAMP should appear to be an important regulatory molecule in the processes of bone formation and resorption.

흰쥐 Nucleus Raphe Magnus에 투여한 Morphine이 척수 Serotonin 유리에 미치는 영향에 관한 연구

이신재,임요한,최영님,김형룡,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.1

It is known that the interaction between opioid neuron and serotonergic neuron in bulbospinal pathway has an important role on pain control. This experiment was perfomed to directly measure the release of serotonin into spinal cord superfusates before and after the microinjection of morphine and its antagonist, naloxone into the NRM of rats. High performance liquid chromatography with electrochemical detection was used to quantitate the amine. The injection of morphine into the NRM induced a significant increase in the amount of serotonin released in the spinal cord, which was reversed after the intraperitioneal injection of naloxone. These results support the proposal that : the analgesia produced by morphine microinjected into the NRM is mediated by the activation of spinally projecting serotonergic neuron.

3-Nicotinonium thiosemicatbazide cephalosporins류의 합성과 항균활성에 관한 연구

이재영,고옥현,강형룡 朝鮮大學校 1996 藥學硏究誌 Vol.18 No.1

For the development of new cephalosporin antibiotics with improved antimicrobial activities , a series of 7β-〔(Z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)acetamido〕-3-〔4-alkyl(or aryl)-3-thisemicarbazid-1-nicotinonium〕methyl-3-cephem-4-carboxylate and 7β-〔(Z)-2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxyimino)acetamido〕-3-〔(4-alkyl(or aryl)-3-thisemicarbazid-1-nicotinonium〕methyl-3-cephem-4-carboxylate were synthesized. Antimicrobial activities of these new compounds were examined against Gram(+) and Gram(-) bacteria. It was found that the synthesized cephalosporin derivatives were good antibacterial activities against Escherichia coil. Almost these compounds were antibacterial activities than cefazoline. These new compounds showed lower activity than cefotaxime against Pseudomonas aeruginosa.

전도성 고분자 센서 어레이를 이용한 휘발성 유기 화합물 가스 인식

이경문,주병수,유준부,황하룡,이병수,이덕동,변형기,허증수 한국센서학회 2002 센서학회지 Vol.11 No.5

휘발성 유기 화합물 가스(Volatile Organic Compounds)를 인식하고 분석하기 위하여 전도성 고분자 센서어레이를 이용한 시스템을 제작하였다. Polypyrrole와 Polyaniline을 화학중합법으로 센서에 전도성고분자막을 형성하였고 이를 통해 VOC 검지용 센서 어레이를 제작하였다. 센서어레이로부터 측정되는 다차원 데이터는 주성분분석법(PCA)과 RBF(Radial Basis Function Network)을 이용하였다. 제안된 시스템으로 VOCs 가스를 인식하는데 있어서 RBF Network이 PCA방식보다 더욱 효율적인 것으로 판단되었다. We fabricated gas recognition system using conducting polymer sensor array for recognizing and analyzing VOCs(Volatile Organic Compounds) gases. The polypyrrole and polyaniline thin film sensors which were made by chemical polymerization were employed to detect VOCs. The multi-dimensional sensor signals obtained from the sensor array were analyzed using PCA(principal component analysis) technique and RBF(radial basis function) Network. Throughout the experimental trails, we confirmed that RBF Network is effective than PCA technique in identifying VOCs.

한국·홍콩·싱가폴 호텔기업의 인터넷웹사이트 비교에 관한 연구

이형룡,홍준 세종대학교 관광산업연구소 2002 호텔관광경영연구 Vol.17 No.-

The purpose of this study is to present more effective ways of using the Internet website for hotel industry in Korea. To achieve the goal of this study attempted to compare Internet website of hotel companies among Korea, Hong Kong and Singapore. The structure of this study is as follows. The first chapter shows the background and the purpose of this study, the second chapter deal with literature review on the general concept of the Internet website and the Internet website as a commercial medium. The third chapter describes Information Flow Model on the Internet website. This is framework for the empirical study classified three stages: the one-way communication stage, the interactive communication stage and the transaction stage, the fourth chapter was delineated the method and the results of the empirical investigation and in the final chapter, it was the overall conclusions of this study. According to the result of this study, Korean hotel companies seems to be using the Internet website as a public relation and customer support tool rather than as a sales tool to create for the actual benefit. To improve a function as a sales tool in Korean hotel companies' Internet website suggestions are: First, to sale hotel gift certificates on the Internet website, second, to develop Internet website considered to offer convenience to customer, finally, to make reliable Internet website, which guarantee secure customer information with expansion of security.

뇌졸중 위험인자를 가진 어지럼 환자에서의 확산강조 자기공명영상

강형구,윤유상,이진희,박인철,이경룡,정상원,구홍두,김승호 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: The management of vertiginous patients is a great challenge to emergency physicians. We evaluated the diagnostic value of a diffusion-weighted image(DWI) in differentiating central vertigo from the peripheral vertigo in patients who presented no neurological symptoms other than risk factors for stroke. Methods: From March 2000 to February 2001, we retrospectively analyzed the cases of 68 patients who visited the emergency department with symptoms of isolated vertigo and who had risk factors for stroke. DWIs, computed tomograms(CT), and medical records were reviewed, and the final diagnose, the DWIs and the CT readings, the risk factors for stroke, and the time it took waiting for a DWI or CT scan were analyzed. Results: Of the 68 patients, 21(30.8%) had central vertigo: 15 vertebrobasilar transient ischemic attacks(VB-TIA), 4 brain stem infarctions, 1 cerebellar infarction, and 1 cerebellopeduncular infarction. The DWI showed a 28.6% sensitivity, a 97.9% specificity, and an 85.7% positive predictive value in diagnosing central vertigo. It also had a 100% sensitivity in detecting infarctions. Conclusion: A DWI had a comparable sensitivity to MRI in detecting central vertigo and small, but potentially, lethal infarctions in our patient population. We recommend clinical application of DWI in the emergency department evaluation of isolated vertigo patients with risk factors for stroke.

운동부하 방법의 차이가 혈당 및 혈중 젖산 농도의 변화에 미치는 영향

채정룡,이갑철,김형준,박정호 군산대학교 체육과학연구소 2000 體育科學硏究所 論文集 Vol.2 No.-

Effect of sub-maximal exercise load differences onthe change of blood glucose and lactate contents were investigated. The results were as follows: 1. Blood glucose is quite relative under exercise load intensity (THR 70%, 90%) right after exercise and at the stage of relaxation. 2. Blood lactate contents were the most immediately after the exercise. 3. Under exercise load (THR 90%), Blood glucose and lactate are less related with each other before and after the exercise; on the other hand, in a restoring stage the result was reverse. Therefore; the higher the exercise intensity is, the more blood lactate is comcentrated immediately after exercise and it talkes a lot longer to go back to the stage of before exercise: the research revealed.

형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

Folate 拮抗劑의 合成硏究

高玉鉉,姜馨龍,李敦日,金永洙,白采善,金京洙 조선대학교 약학연구소 1990 藥學硏究誌 Vol.12 No.1

In order to synthesize Methotraxate(MTX) analogues, author synthesized 3-〔〔〔2-(N-Ethyl-N-tosyl-p-aminobenzenecarboxamido)ethyl〕thio〕methyl〕-2-methylimidazo-〔1,2-a〕pyridine(15), 2-(N-Ethyl-N-toyl-p-aminobenzoyl)-5-phenyl-2H-tetrazole(16),2-(N-Ethyl-N-tosyl-p-aminobenzoyl)-5-(nitrobenzyl)-2H-tetrazole(17), 3-〔〔〔2-(N-Ethyl-p-aminobenzenecarboxamido〕ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridine(18), 2-(N-E-thyl-p-aminobenzoyl)-5-phenyl-2H-tetrazole(19), 2-(N-Ethyl-p-aminobenzoyl)-5-(4-nitro-benzyl)-2H-tetrazole(20), 2-Amino-3-cyano-5-〔3-〔〔〔2-(N-ethyl-p-aminobenzenecarboxa-mido)ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridino〕methyl pyrazine(21), 2-Amino-3-cyano-5-〔2-N-ethyl-p-aminobenzol)-5-phenyl-2H-tetrazol-2-yl〕methylpyrazine(22), 3-A-mino-3-cyano-5-〔2-N-ethyl-p-aminobenzol)-5-(4-nitrobenzyl)-2H-tetrazol-2-yl〕methylp-yrazine(23), 2,4-Diamino-6-〔3-〔〔〔2-(N-ethyl-p-aminobenzenecarboxamido)ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridino〕 methylpteridine(24), 2,4-Diamino-6-〔2-(N-ethyl-p-aminobenzoyl)-5-phenyl-2H-tetrazol-2-yl〕methylpteridine(25), and 2,4-Diamino-6-〔2-(N-ethyl-p-aminobenzol)-5-(4-nitrobenzyl)-2H-tetrazol-2-yl〕methylpteridine(26), respectively.

미만성 축삭 손상 환자에서의 예후 인자

음성화,임동준,김봉룡,조태형,박정율,서중근,이기찬,이훈갑 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.12