Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

세로방향의 휜이달린 환형관 내의 유체흐름에 대한 연구

류진형,정인권,김정식,김내현 國立金烏工科大學校附設生産技術硏究所 1993 産業技術開發硏究 Vol.9 No.-

In this study, the pressure drop and velocity profile were measured in longitudinally finned annulus. The test specimens were a round rod of 38㎜diameter with no fin, a rod a 40㎜ with 6 fin, a rod of 40㎜ with 8 fin, and a rod of 50㎜ with 8 fin. The fin thickness was 3㎜ and the fin height was 5㎜. The specimen was installed in a tube of 76㎜ inner diameter. For the round rod with no fin, the pressure drop data were well predicted by Blasius correlation, and the velocity profile was very close to the Nikuradse's non-dimensional velocity profile. For finned rods, the pressure drop of 8 fin rods was well predicted by Blasiue correlation. However, the correlation slightly under-predicted 6 fin data. The maximum velocity points in the finned annulus were circumferentially uniform except for the fin top position. Quarmby's equation [Eq.14] predicted the maximum velocity points excellently.

대전공단지역의 대지중 독성 금속의 농도 분석

류영태,남병현,이진흥 忠南大學校 環境問題硏究所 1997 環境硏究 Vol.15 No.-

In spite of lower concentration of toxic metals in urban air, the magnitude of health risk associated with may not be smaller than that by other airborne hazardous pollutants. Especially, it has been expected that the risk from an industrial complex may be much higher by toxic metals including carcinogenic ones. However, unfortunately there has been few domestic research on the concentration profile and risk assessment of airborne toxic metals in such area. The reason may be that the final objective of studies performed before for our urban air was either source characterization or quantitative source estimation of suspended particulate matter and development of a receptor model. Thus, few investigation into the concentration distribution for toxic metals such as As, Be , Cr, Ni, Se, Sb and V has been done. Therefore, this research will focus on 2-year- analysis(twice or four times per month) by using ICP-MS and ICP-AES for the concentration of heavy metals in Taejon Industrial Complex. ICP-MS has much more excellent detection limits and reproducibility for many toxic metals than any other analytical technique. Total suspended particulates were collected on quartz fiber filters by a high volume air sampler. About 20 metal elements including 13 toxic ones were analyzed by ICP-MS and ICP-AES after the pretreatment of mixed acid extraction. As a part of the research, the following results for the period of from June to August, 1997 were summarized : (1) the geometric mean concentration of TSP was 87.28㎍/㎥ during the sampling period in the study area. The maximum was 114.16㎍/㎥ in the 4th week of August while the minimum was 47.97㎍/㎥in the first week of August; (2) much higher concentrations of Pb and Zn were noted among toxic metals and their geometric means were 288.31ng/㎥ and 153.47ng/㎥, respectively. The highest concentration of Pb was 570.99ng/㎥ in the 4th week of August while that of Zn was 279.03 ng/㎥ in the first week of June;(3) the concentration of each Ni, Mn, Zn and Fe was found to be highly correlated with that of TSP, which indicated the greater impact of man- made pollution in the area. The concentration of elements such as Al, Ca and Mg related to soil and road dust was found to be the highest in June and their correlation coefficients were estimated to be very high.

Filteraton 位相空間에서의 거리화와 완비성에 관하여

유근식,현진오 제주대학교 1982 논문집 Vol.14 No.-

In this paper,we treat to the problem of metrization for filteration topology and find to the condition for the completion of the filteration topology.

하이퍼 큐브네트워크를 이용한 Radiance Estimation 프로세서의 설계

류현우,김영진,이현수 경희-다반 ASIC 설계교육센터 2005 경희-다반 ASIC센터 논문집 Vol.6 No.-

본 논문에서는 Radiance Estimation 연산에 존재하는 전역적인 병렬성을 처리할 수 있는 구조를 제안한다. 기존의 소프트웨어 처리는 계산에 필요한 많은 연산의 시간이 필요로 하기 메문에 하드웨어 적인 구현의 필요성이 증대하였다. Radiance Estimation 알고리즘의 병렬성을 이용하여 SIMD 처리 방식으로 설계를 하였으며 연산의 단위인 Cell의 통신은 4차인 하이퍼 큐브 네트워크를 이용하여 데이터간의 효율성은 높였다. 그리고 연산의 신호에 따라 다양한 기능을 하는 Cell로 구성을 하여 Radiance Estimation 연산의 Photon 검색과 밀도추정 연산을 하나의 시스템에서 적용이 가능하였다.

회수수에 당류계 안정화제를 처리한 시멘트 모르터의 특성

金虎林,우종완,한민철,류현기,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this paper, fundamental properties of cement mortar using recycled water with stabilizing agent are discussed in order to investigate the applicability of recycled water. Stabilizing agent content, solid contents and open time are varied in this paper. For fresh mortar properties, highest fluidity with 0.15% of stabilizing agent was observed and air content was increased with an increase in stabilizing agent contents. Solid contents and open time of recycled water had nothing to do with fluidity and air content loss. Little difference in setting time was found with the presence of stabilizing agent and without, while it was accelerated with an increase in open time and solid content. For strength properties, the highest strength value was observed at 0.15% of stabilizing agent. An increase in solid contents resulted in an enhancement of strength, while strength decreased with an increase in open time and elapse of age. Drying shrinkage exhibited a reduction with an increase in stabilizing agent contents and open time. From the present paper, the combination of 0.15% of sacharic based stabilizing agent, 3% of solid content and 1day of open time is found to be an optimal condition to perform effectively.

한국인의 치과의원 내원요인 조사 연구

류영욱,배현숙,성진효,김동기 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.1

According to the increase of the number of dental clinics and the level of the patient's recognition about oral health care, the need for effective clinical management to provide proper oral health care service to the patients has arisen. Many patients has selected the dental clinics based on self estimation or other recommendation. Therefore the main reasons for selecting the dental clinic by the patients needed to analyze, So we conducted the survey which prepared questionnaire were provided to 700 subjects and collected data related to the factors for patients to select the dental clinics. The results were as follows : 1. In the view of access, the place easy of access and parking capacity was important to the degree of 43.0% and 30.8%, respectively. 2. In relation to the dentist, the quality of treatment and the trust was important to the degree of 80.7% and 71.3%, respectively. 3. Modern clinical facility was occupied 65.5% in the basic environment factors. 4. In the basic factors, kindness and cleanliness was 76.6% and 76.7%, respectively, and it showed that these factors were more important than the number of the clinicians and comfort of the waiting room. 5. In relation to the satisfaction of treatment, cheaper treatment cost, decreased waiting tine, the variety of treatment tine and various payment method were important to the degree of 51.8%, 48.4%, 42.8% and 39% in order.

SOM 신경망을 이용한 심전도 검출에 관한 연구

崔塡亨,柳宗賢,白承和 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In This study, proposes a self-organizing QRS-wave detection algorithm for ECG using Kohonen Neural Networks and a new preprocessing algorithm for R-point detection. The implemented ECG reading system consists of a preprocessor, a SOM and a QRS-detector. The preprocessor detect R-point in the ECG. The SOM organizes training vectors into a pattern book, and the QRS-detector executes Q and S-point detection with the pattern book. Without an averaging process permits a faster operation and applying a nonlinear peak detector to a preprocessor. The self-organizing ability of SOM has proven to be a good solution for recognizing and organizing QRS-wave patterns. Moreover, The SOM prevents an loss of information such as signal distortion due to a mathematical transform. The QRS-detector based on the pattern book from the SOM executes an efficient ECG reading compared to the other methods.

기관내 삽관 후 발생한 기관 괴사와 협착증 : 증례보고 A case report

권진형,구자욱,유시현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Tracheal necrosis and stenosis are rare complications of prolonged endotracheal intubation. A 62 years old female was performed cerebral aneurysmal neck clipping under general anesthesia via endotracheal intubation and transferred to the 12th intensive care unit. The patient was mechanically ventilated and tracheostomy was done on the postoperative day. Necrotic tissue, pus and stenosis were found around the ballooning site of the trachea. T-tube insertion was done on the both postoperative day and the patient was discharged on the 80th postoperative day.

스키시즌권 구매자들의 스키장별 선호도 조사

장현철,김경식,방준현,최천진,엄정식,김응준,이루지 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to examine the perception and preference of skiers in a bid to provide ski resorts with useful information about successful positioning strategy. The subjects in this study were 9,722 skiers who purchased season tickets from N web site that sold ski season tickets. Their online purchasing route and page view were analyzed to find out what factors affected their purchase of ski season tickets. The findings of this study were as follows: First, 78 percent of the people investigated were make, and 22 percent were female. By age, the largest group was in their 20s(5,344 skiers), followed by those in their 30s(3,434), those in their 40s(631) and teenagers(229). Second, their page view rate with Yangji recorded highest with 24%, followed by Sungwoo Resort with 23% and Chonmasan with 13 percent. Third, by geographic area, the skiers from southern Gyeonggi province bought the largest number of tickets for the 2002/2003 season, followed by Gangweon province residents and northem Gyeonggi province dwellers. In the case of the 2003/2004 season, those from Gangweon provinece pruchased the most tickets, followed by southern Gyeonggi province dwellers and north Gyeonggi province residents.