Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

초등교사의 열정에 대한 인식: 의미연결망 분석의 활용

임효진 ( Hyo Jin¸ Lim ),최은혜 ( Eun Hye¸ Choi ),박호익 ( Ho Ik¸ Park ),이지현 ( Ji Hyun¸ Lee ) 한국초등교육학회 2021 초등교육연구 Vol.34 No.3

본 연구에서는 초등교사가 가지고 있는 열정에 대한 인식구조를 파악하기 위해 현직 초등교사 131명을 대상으로 설문조사를 실시하였다, 연구에서는 열정, 열정의 장단점, 교사열정에 대한 인식구조를 알아보기 위해 의미연결망 분석을 활용하여 근접 중심성, 연결중심성, 매개중심성, 아이겐벡터 중심성을 위주로 살펴보았다. 연구결과 첫째, 열정에서는 ‘힘’,‘강렬’과 같은 높은 강도나 에너지를 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 열정의 행동, 정서, 동기 특성에 대한 주제가 분류되었다. 둘째, 열정의 장점에서는 ‘삶’,‘원동력’,‘에너지’등이, 열정의 단점에서는 ‘타인’,‘피로’,‘살피지 못함’등이 중요한 역할을 하는 것으로 보아 기존 이론에 나타난 열정의 특성을 설명하고 있었다. 셋째, 교사열정에서는 ‘학생’,‘수업’과 같은 교수활동의 실천을 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 교육활동 전반, 학생과 수업, 전문성 개발에 대한 주제가 분류되었다. The purpose of this study was to explore the meaning of passion perceived by elementary school teachers. In order to understand the structure of perception of passion, we surveyed 131 in-service elementary school teachers. Responses to passion, the positive and negative role of passion, and teacher passion were analyzed using a semantic network analysis. We focused on closeness centrality, degree centrality, betweenness centrality and eigenvector centrality. Results showed that first, in passion, words indicating high strength and energy such as‘power’and‘intensity’played an important role in the structure of perception. Themes on behavioral, emotional, and motivational characteristics of passion were classified as sub-factors of passion as a result of community analysis. Secondly,‘life’,‘motivation’, and‘energy’appeared in key-word analysis in the positive role of passion while‘others’,‘fatigue’, and‘not caring for’appeared in the negative role of passion, reflecting the theory on the dualistic model of harmonious and obsessive passion. Third, in teacher passion, words indicating teaching activities and their practices such as‘student’and‘instruction’played an important role in the structure of perception. Themes related to educational activities in general, students and instruction, and professional development were classified as sub-factors of teacher passion.

장기 알콜투여가 생쥐의 간 및 신장, 위장 조직 손상에 미치는 영향

김진택,김동환,안상현 東國大學校韓醫學硏究所 1994 東國韓醫學硏究所論文集 Vol.3 No.-

Alcohol is a major risk factor for several diseases and excessive, long-term alcohol consumption are cause physical alteration-fatty liver, hepatitis, cirrhosis, breaking down, Wernicke-karasakoffs syndrome, weight loss, and poor immunity-in virtually all organ and tissue, This study was observed that liver, kindney, and stomach were altered in mouse by the effect of chronic alcohol administration. The mouse were sacrificed to obtain the tissue after mouse were orally injected with 25% ethanol 18㎖/kg/day for 120days. The tissue were stained by hematoxylin and eosun and then obseved by light microscope. The results of this study were as follows : 1. The congestion was appeared in liver after 120days alcohol administration 2. The destruction of glomerulus were increased and parietal cell of Bowman's capsule were swelled such as cuboidal cell after 12days alcohol administration. The congestion was appeared in alcohol administrated group. 3. The mucosa and gasstric pit were destructed and the ulceration was appeared in stomach after 120days administration. The parietal cells and chief cells were damaged. Above results were shown that the tissue were damaged by chronic alcohol administration.

ICP-AES를 이용한 대기분진 중 중금속 분석에 대한 정도관리

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2003 環境硏究 Vol.21 No.-

In this study, factors of quality control such as laboratory environment, capability of instrument/equipment, method of analysis, calibration, and standard solution were investigated to obtain quality assurance when analyzing airborne heavy metals (e.g., Pb, Cr, Cd, Cu, Mn, Ni, Fe) using ICP-AES. A procedure of quality assurance and evaluation method of uncertainty are also suggested by applying ISO Guide. The suggested process of quality assurance will be helpful for the national monitoring network to produce better data.

기기 중성자방사화분석법을 이용한 황사특성

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

Using INAA, which has been thought to be one of the best analytical techniques for airborne particulate matters, this study centered on the quantitative analysis of 32 metal elements within Asian Dust aerosols. Concentrations of REEs of crustal origin, such as Ce, Cs, Dy, La, Sc, and Sm were more than 8.5 times higher in Asian Dust aerosols. In addition, major crustal elements, such as Al, Ca, Co, Fe, K, Mg, Mn, and Ti showed more than 3 times higher concentrations in those aerosols, which made PM 10 and all metal concentrations be also about 3.0 and 4.8 times higher, respectively. However, concentrations of anthropogenic origin, such as As, Br, Ci, I, In, Sb, Se, and Zn were increased to be a little higher, which means no great effect of Asian Dust on these elements.

북미자유무역협정(NAFTA)의 출범과 미국노사관계의 변화

임현진,최종철,홍성태 서울대학교 국제지역원 1996 국제지역연구 Vol.5 No.4

탈냉전 시기 미국의 새로운 국제전략은 세계 각국의 불균등한 발전상황을 적절히 이끌어 새로운 팍스 아메리카나의 시대를 열겠다는 명확한 목표를 가지고 있다. 기술 및 산업의 차원에서 이 목표는 정보통신분야의 주도권 장악으로 추진되고 있으며, 세계무역질서의 차원에서는 WTO와 NAFTA의 구축으로 젼개되고 있다. 여기서 NAFTA는 두가지 함을 갖는다. 첫째, 궁극적으로 지구적 지배력을 재구축하기 위한 지역적 발판을 마련하는 것이다. 둘째, 발전도상국과 선진국의 전면적 자유무역이 개신된다는 것이다. 미국 노사관계의 변화와 관련해서는 특히 두 번째 측면에 주목할 필요가 있을 것이다. 그러나 현재 진행되고 있는 미국 노사관계의 변화는 NAFTA 자체보다는 포드주의의 위기와 다른 선진자본주의국과의 경쟁에서 더 큰 영향을 받고 있는 것으로 판단된다. 즉 미국적 노사관계를 대표해 온 뉴딜형 노사관계와 실리적 조합주의의 한계에 대한 인식은 바로 이것들이 기반하고 있는 포드주의에 대한 비판과 대체의 과정에서 필연적으로 나타나고 있는 것이다. 이와 관련하여 NAFTA는 미국의 노동조합이 종래의 보호주의적 외투를 벗어버리고 개발도상국 노동자들과의 연대를 적극적으로 추구해야 할 필요성을 강화한다. 포스트 포드주의를 배경으로 미국의 자본과 정부가 주도하고 있는 실리적 조합주의에서 참여적 조합주의로의 변화에 대해 미국의 조동조합이 얼마나 적극적으로 대처할 수 있느냐의 문제는 새로운 국제적 연대에 얼마나 적극적으로 대처할 수 있느냐의 문제와 깊은 상관관계를 가질 것이다. The international strategies of America in the post cold-war period have the apparent objective that America will open a new pax-Americana age, by leading the unequal developmental situations among every countries in the world. This objective is propelled through holding the initiative in the field of information and communication in terms of technology and industry, and is developed by the establishment of the WTO and NAFTA in terms of the world trade order. Here, NAFTA has two implications; first, preparing for a regional platform so that America will be able to re-establish its global power; secon, the overall free-trade between advanced countries and developingcountries is beginning. With regardto the change of America's capital-labour relations, we need to call our attention to the second aspect. But it seems that the change of America's capital-labour relations in progress is more affected by the competition with other advanced countries than by NAFTA. That is, the recognition of the limits of the New-Deal capital-labour relations up to the present is necessarily emerging in the process of cirticizing and replacing Fordism which is regarded as a basis for both. Accordingly, NAFTA is strengthening its position on the necessity for labour in the US to actively pursue solidarity with unions in developing countries, taking off its old protectionist cloat. As for unions in America, how to cope with the transition from business unionism to participatory unionism which America's capital and government are leading in the back-ground of post-Fordism will be closely related with how actively to pursue a new international solidarity.

다발성 외상환자에서 필수적 단순 방사선검사의 의의

임경수,황성오,임종천,이부수,이강현,이진웅 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

In the guidelines of Advanced Trauma Life Support(ATLS), essential plane roentgenograms (chest AP vIew, cervical spine lateral view and pelvis AP view) are recommanded when primary survey and resuscitation phase are finished in care of multiple injured patients. A prospective study was carried on 376 multiple trauma patients who admitted to Wonju Christian Hospital from July, 1995 to October. We analyzed clinical significance of essential X-ray in multiple trauma patients, and classified the patients by their status of mentality. The one is alert group when their Glasgow Coma Scale(GCS) is over 13 point, the other is disoriented mentality group when their GCS are below 12 point. The alert group was redivided by presence of clnical symptoms and/or signs, and clinical significances of essential X-ray were analyzed. Among the 376 patients, 330 patients are alert group that their GCS score is over 13 point and 46 patients are disoriented group who's GCS were below 12 point. The detection rate of abnormality in essential X-ray is higher in disoriented group than alert group(cervical-spine lateral view : 13.5% vs 3.0% cheat AP view : 0.4%, pelvis AP view : 0%). This study suggests that their are no needs of taking essential X-ray in patients who is alert in mental state and have no clinical symptoms and/or signs.

저작근 공간에서 재발된 치성각화낭

임수연,허경회,이원진,최현배,최순철 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.2

The odontogenic keratocyst (OKC) is a developmental odontogenic cyst typically occurring in the jaws. Since the first description of OKC was published in 1956, the lesion has been of particular interest because of its specific histopathologic features, high recurrence rate, and aggressive behavior. Recurrences most commonly arise within bone at the site of the original cyst. However, as lining cells may find their way into surrounding tissues either from implantation during surgery or from cortical perforation recurrences may arise at a distance from the original cyst. Here, we report a rare case of recurrent OKC which was first developed in mandible and recurred within the masticatory space.

리스페리돈과 올란자핀이 투여된 정신과 환자들에서 간 효소 변화에 대한 후?적 연구

임현국,배치운,박석준,김정진,이창욱,이수정,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.3

7β-Hydroxycholesterol이 사람 백혈암에 대한 세포 독성 효과

임희경,현진원 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) has been evaluated using various leukemia cell lines by MTT test. Among the tested cell lines, 7β-OHC showed to be more cytotoxic to THP-1, human monocytic leukemia cell line. IC50 value of 7β-OHC on THP-1 was 10μM. Kinetic of on THP-1 showed that growth of THP-1 began to decrease at 36 h after treatment 7β-OHC. 7β-OHC induced apoptosis shown by DNA fragmentation by gel electrophoresis. 7β-OHC induced apoptosis through down-regulation of Bcl-2 expression. Taken together, these results suggested that treatment with 7β-OHC inhibits the proliferation of THP-1 cells through apoptosis.