Lignans from the Fruits of Schizandra chinensis and Their Inhibitory Effects on Dopamine Content in PC12 Cells

Seon-Mi Seo,Hak-Ju Lee,Youngki Park,Myung Koo Lee,Jae-In Park,Ki-Hyon Paik 한국생약학회 2004 Natural Product Sciences Vol.10 No.3

Five lignans including gomisin N (1), wuweizisu C (2), gomisin L1 (3), (+)-deoxyschizandrin (4), and gomisin J (5) have been isolated from the fruits of Schizandra chinensis. The structures of the isolated compounds were elucidated by analyzing MS and NMR spectra. Effects of the compounds isolated in this study on the dopamine content in PC12 cells were investigated to evaluate their inhibitory effectiveness. The gomisin N, wuweizisu C, and gomisin J showed 25.4%, 39.8%, and 35.1%, respectively, inhibition effect on dopamine content in PC12 cells at the concentration of 50 mg/ml.

소의 간 시토졸 알데히드 탈수소효소의 정제와 특성에 관한 연구

구현서,주충노 ( Hyon Seo Koo,Chung No Joo ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5

Cytosolic aldehyde dehydrogenase (ALDH) of bovine liver was purified by ammonium sulfate precipitation, DEAE-Sephacel column chromatography, hydroxyapatite affinity chromatography and 5`-AMP Sepharose 4B affinity chromatography. The molecular weight of cytosolic ALDH was determined to be 205,000 dalton by Sephadex G-150 gel filtration. SDS-polyacrylamide gel electrophoresis showed that it was homotetramer of four identical subunits, molecular weight of each of which was 52,000 dalton. Optimal pH and optimal temperature of this enzyme was found to be pH 9.0 and 37℃ respectively. The isoelectric point of this enzyme was 6.3. K_m values of bovine liver cytosolic ALDH for acetaldehyde, propionaldehyde, succinic semialdehyde was high (4∼6 mM) but those for benzaldehyde and indole 3-acetaldehyde were extremely low (20∼50 μM). This result suggested that bovine liver cytosolic ALDH might contribute partly to acetaldehyde oxidation in ethanol metabolism but its major function would be to oxidize biogenic aldehydes. It was realized that the esterase activity of bovine liver cytosolic ALDH was inhibited by propionaldehyde, a substrate of the dehydrogenase activity of this enzyme, suggesting that the catalytic site of dehydrogenase and esterase activity might be identical. Chloral hydrate protected N-ethylmaleimide inhibition of both dehydrogenase and esterase activity of this enzyme. This again suggested that both active sites might be identical. v

쥐간 미토콘드리아의 외막결합 Aldehyde Dehydrogenase 의 활성부위에 관한 연구

구현서,주충노 ( Hyon Seo Koo,Chung No Joo ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5

The outer membrane bound ALDH of rat mitochondria was purified and its reaction phenomena were investigated through chemical modifications. The purified membrane bound ALDH was inhibited up to about 50% by disulfiram when its concentration was high and it was realized that at 0.1∼0.4 mM of sulhydryl specific iodoacetamide concentration, the ALDH was inhibited by the formation of enzyme inhibitor complex in one to one ratio of molecular level. The second-order rate constant was 334.3 M^-1min^-1. The purified outer membrane bound ALDH was also rapidly inactivated by thiol specific reagent such as phydroxymercuribenzoate at the concentration of 0.2∼0.6 μM. Inactivation by p-hydroxymercuribenzoate was protected by acetaldehyde, a substrate of ALDH. The second-order rate constant for the inactivation was calculated to be 773,000 M^-1min^-1, and the reaction order with respect to p-hydroxymercuribenzoate was 1.08, indicating that there might be one sulfhydryl residue per enzyme. Arginine specific reagent such as phenylglyoxal, histidine specific reagent such as diethylpyrocarbonate and lysine specific reagent such as pyridoxal 5`-phosphate have no effect on the above ALDH. However, phenylmethylsulfonyl fluoride, a serine specific reagent, inactivated ALDH as much as 66%. These results indicated that the cysteine, serine residues might probably be located at/or near the active site of the enzyme.

Effects of (+)-Eudesmin from the Stem Bark of Magnolia kobus DC. var. borealis Sarg. on Neurite Outgrowth in PC12 Cells

Yang, Yoo-Jung,Park, Jae-In,Lee, Hak-Ju,Seo, Seon-Mi,Lee, Oh-Kyu,Choi, Don-Ha,Paik, Ki-Hyon,Lee, Myung-Koo The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.12

(+)-Eudesmin [4,8-bis(3,4-dimethoxyphenyl)-3,7 -dioxabicyclo[3.3.0]octane] was isolated from the stem bark of Magnolia kobus DC. var. borealis Sarg. and found to have neuritogenic activity. $50\;{\mu}M$ (+)-eudesmin induced neurite outgrowth and enhanced nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. At this concentration, (+)-eudesmin also enhanced NGF-induced neurite-bearing activity and this activity was partially blocked by various protein kinase inhibitors. These included PD98059, a mitogen-activated protein kinase (MAPK) kinase inhibitor. GF109203X, a protein kinase C (PKC) inhibitor and H89, a protein kinase A (PKA) inhibitor. These results suggest that (+)-eudesmin can induce neurite outgrowth from PC12 cells by stimulating up-stream MAPK, PKC and PKA pathways.

Aldehyde Dehydrogenase 의 반응 메카니즘에 관한 연구 - Dehydrogenase 활성의 속도결정 단계의 추구

주충노,김두식,구현서,김주희 ( Chung No Joo,Doo Shik Kim,Hyon Seo Koo,Joo Hwee Kim ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.6

The effect of several ions and chemical modification experiments using bovine liver cytosolic aldehyde dehydrogenase (ALDH) and human erythrocyte ALDH have been carried out to understand the rate-limiting process of dehydrogenase activity. From the previously reported experimental results and this study suggested that the releasing step of NADH from Enzyme-NADH complex might be a rate-limiting process.

Aldehyde Dehydrogenase의 반응 메카니즘에 관한 연구 -Dehydrogenase 활성의 속도결정 단계의 추구-

주충노,김두식,구현서,김주휘,Joo, Chung-No,Kim, Doo-Shik,Koo, Hyon-Seo,Kim, Joo-Hwee 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.6

소의 간 aldehyde dehydrogenase(ALDH)와 사람 적혈구 ALDH에 미치는 금속이온의 영향과 화학변형 실험을 행하고 보고된 ALDH의 반응 메카니즘에 관한 실험결과들을 종합하여 광범위한 특이성을 가진 일반 ALDH의 dehydrogenase 활성의 속도제한 단계가 Enzyme-NADH complex로부터의 NADH의 해리단계임을 알 수 있었다. The effect of several ions and chemical modification experiments using bovine liver cytosolic aldehyde dehydrogenase (ALDH) and human erythrocyte ALDH have been carried out to understand the rate-limiting process of dehydrogenase activity. From the previously reported experimental results and this study suggested that the releasing step of NADH from Enzyme-NADH complex might be a rate-limiting process.

24시간 활동성 혈압과 임상 혈압과의 비교 및 좌심실 비대와의 상관관계

박승훈 ( Seung Hoon Park ),방덕원 ( Duk Won Bang ),서존 ( John Seo ),홍성욱 ( Sung Wook Hong ),김도회 ( Do Hoi Kim ),윤여준 ( Yeo Joon Yoon ),안지훈 ( Ji Hoon Ahn ),현민수 ( Min Su Hyon ),김성구 ( Sung Koo Kim ),권영주 ( Young Jo 대한내과학회 2007 대한내과학회지 Vol.72 No.2

목적: 고혈압을 진단함에 있어 24시간 활동성 혈압 측정이 임상 혈압 측정보다 정확하다고 알려져 있지만, 아직까지 국내에서는 24시간 활동성 혈압과 임상 혈압의 관계에 대한 연구는 드문 실정이다. 본 연구는 24시간 활동성 혈압과 임상 혈압을 비교하고, 심전도에서의 좌심실 비대와의 연관성을 알아보고자 하였다. 방법: 혈압 평가를 의뢰받고 항고혈압제재의 복용력이 없는 건강한 성인 203명(남 108, 여 95)을 대상으로 임상 혈압을 3회 측정 후 평균치를 구하였고, 24시간 활동성 혈압을 측정하였다. 24시간 활동성 혈압 중 낮시간 평균 혈압을 임상 혈압과 비교분석하였다. 이들 환자의 심전도를 검사하여 V1의 S파의 전압 크기와 V5의 R파의 전압 크기를 합하여 각 혈압과의 상관관계를 조사하였다. 결과: 전체 대상군의 낮시간 활동성 혈압의 평균은 수축기 혈압 135.33±13.73 mmHg, 이완기 혈압 86.55±11.42 mmHg였고, 임상 혈압의 평균은 수축기 혈압 140.10±17.41 mmHg, 이완기 혈압 88.84±10.14 mmHg로 임상 혈압이 낮시간 활동성 혈압보다 수축기 혈압은 5 mmHg, 이완기 혈압은 2 mmHg 정도 높았다(p<0.001). 임상 혈압의 고혈압 기준치를 140/90 mmHg라고 할 때 여기에 상관된 낮시간 활동성 혈압의 예측치는 135/87 mmHg였다(p<0.001). 임상 혈압 측정에서 고혈압으로 진단된 군 중에서 24시간 활동성 혈압 측정이 정상으로 나온 백의 고혈압(isolated office or white-coat)의 발생률은 10.8%였다. 심전도에서의 전압크기는 24시간 활동성 혈압 중 낮시간 수축기 혈압, 이완기 혈압과 밤시간 수축기 혈압, 이완기 혈압과 모두 유의한 양의 상관관계를 보였으며, 특히 낮시간 수축기 혈압과 가장 높은 상관관계를 보였다(r=0.283, p<0.001). 결론: 임상 혈압과 24시간 활동성 혈압간에 유의한 차이가 있었고, 낮시간 활동성 혈압의 정상 상한치는 135/87 mmHg였으며, 심전도에서 좌심실비대를 반영하는 좌심실 전압의 크기는 24시간 활동성 혈압 중 낮시간 수축기 혈압과 가장 높은 상관관계를 보였다. Background: This study compared the results of 24 hour ambulatory blood pressure monitoring with the clinical blood pressure measurements, and we investigated the relationship of the blood pressure measurement and left ventricular hypertrophy, as determined by routine 12 lead electrocardiography. Methods: We studied 204 healthy adults with no prior history of heart disease or antihypertensive medication. The clinic blood pressure was measured 3 times and the average was taken. We compared the clinic blood pressure with the daytime blood pressure of the 24 hour ambulatory blood pressure monitoring, and we compared the blood pressure with the sum of the voltage of the S wave on V1 and the R wave on V5. Results: The average of the daytime ambulatory blood pressure of all the patients was 135.33±13.73 mmHg for the systolic pressure and 86.55±10.14 mmHg for the diastolic pressure. The average of the clinic blood pressure measurement was 140.10±17.41 mmHg for the systolic pressure and 88.84±10.14 mmHg for the diastolic pressure. The clinic blood pressure averaged higher than the daytime ambulatory blood pressure by 5 mmHg on the systolic pressure and 2 mmHg on the diastolic pressure (p<0.001). The normal ambulatory blood pressure limits were estimated as those that best correlated with 140/90 mmHg at the clinic. The estimated value was 135/87 mmHg for the daytime ambulatory blood pressure (p<0.001). The incidence of white coat hypertension was 10.8%. The sum of the voltage on electrocardiography showed a positive linear relationship with all the blood pressure measurements. The daytime systolic blood pressure showed the strongest correlation with the 24 hour ambulatory blood pressure monitoring (r=0.283, p<0.001). Conclusions: We found a linear relation and we analyzed the differences between the clinical and 24 hour ambulatory blood pressure. A daytime ambulatory blood pressure value of 135/87 mmHg was a suitable upper normal limit for the corresponding cutoff value of the clinic blood pressure. Left ventricular hypertrophy showed the strongest relationship with the daytime systolic blood pressure among the results of the 24 hour ambulatory blood pressure monitoring. (Korean J Med 72:181-190, 2007)

항산화제 투여 후 총 항산화지수 및 사이토카인 변화와 스텐트 재협착에 미치는 영향

윤형근 ( Hyung Geun Yoon ),방덕원 ( Duk Won Bang ),박승훈 ( Seung Hoon Park ),안지훈 ( Ji Hoon Ahn ),서존 ( John Seo ),윤여준 ( Yeo Joon Yoon ),현민수 ( Min Su Hyon ),김성구 ( Sung Koo Kim ),권영주 ( Young Joo Kwon ) 대한내과학회 2006 대한내과학회지 Vol.71 No.2

목적: 관상동맥질 환자에서 항산화 비타민 C와 E, 항산화제 probucol 병합요법이 스텐트 시술 후 혈중 P-selectin, chemokine, 사이토카인의 농도변화와 스텐트 재협착에 미치는 영향을 연구하였다. 방법: 관상동맥 질환으로 일반 스텐트 시술을 시행한 총 90명의 환자를 항산화제를 복용하지 않은 대조군과 비타민 C와 E, Probucol을 병합 투여한 군으로 나누었으며 이 중 6개월 후 추적관상동맥 조영술을 시행한 환자 71명을 대상으로 비교하였다. 항산화제 치료 전과 6개월 후 혈장내 전체 항산화지수, P-selectin, MCP-1, IL-6과 IL-10을 측정 비교하였고, 6개월 후 관상동맥 조영술을 시행하여 재협착 유무를 조사하였다. 결과: 스텐트 시술 전 임상적 소견, 위험인자, 생화학지표 등은 양 군 간에 유의한 차이가 없었으며 전체 항산화지수, P-selectin, MCP-I, IL-6, IL-10도 양 군 간 유의한 차이가 없었다. 6개월 후 추적검사에서 전체 항산화 지수 P-selectin, MCP-I, IL-6 및 IL-10 역시 양 군 간 유의한 차이가 없었고, 재협착 발생률도 양 군 간 유의한 차이가 없었다. 결론: 관상동맥 질환에서 일반 스텐트 시술 직후 비타민 C와 E, Probucol 병용투여는 혈장내 항산화 상태와 심혈 관의 죽상경화 진행에 관여하는 여러 가지 화학물질의 농도와 스텐트내 재협착 예방에 영향을 미치지 못했다. Background: Oxidative stress might be a role in atherosclerosis and increased intake of antioxidant appear to be protective and modify neointimal formation. An antioxidant and probucol prevents endothelial dysfunction and low density lipoprotein oxidation and also inhibits the secretion of cytokine by macrophages. We aimed 1) to study the effects of antioxidant (Vitamin C, E and probucol) supplementation on serum level of antioxidant status (TAS), P-selectin, MCP-1, IL-6 and IL-10 and 2) to investigate the effects of antioxidant intake on in-stent restenosis. Methods: Total 90 patients were assigned to control or antioxidant group (probucol; 500 mg, vitamin C; 1,000 mg, vitamin E; 400 mg). We performed follow up coronary angiography in 35 patients of antioxidant group and 36 patients of control group after 6 months of coronary bare metal stent implantation. We counted the stenotic lesions more than 50% of implanted stent lumen as a restenosis by quantitative coronary angiography. The serum levels of total antioxidant status, P-selectin, MCP-1, IL-6 and IL-10 were measured. Results: The serum levels of total antioxidant status was not elevated in antioxidant group. Antioxidant supplementation did not change the serum levels of P-selectin, MCP-1, IL-6 and IL-10. The 6-month angiographic in-stent restenosis rate was 27% versus 30% (p=NS) with an associated late loss of 0.76±1.01 mm versus 0.91±1.00 mm (p=NS) for antioxidant group and control group. The serum levels of total antioxidant status did not correlate with the restenosis or late loss after stent implantation. Conclusions: Vitamin C, E and probucol did not elevate the serum level of antioxidant status and could not prevent in-stent restenosis after bare metal stent implantation.(Korean J Med 71:158-165, 2006)

관동맥 심질환 환자에서 Paraoxonase 유전자 2형의 유전자형 빈도에 대한 연구

최태윤,이유경,김원배,이동화,현민수,서세웅,김성구,권영주 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.4

Use of coliphages to investigate norovirus contamination in a shellfish growing area in Republic of Korea

Cho, Kyuseon,Lee, Cheonghoon,Park, SungJun,Kim, Jin Hwi,Choi, Yong Seon,Kim, Man Su,Koo, Eung Seo,Yoon, Hyun Jin,Kang, Joo-Hyon,Jeong, Yong Seok,Choi, Jong Duck,Ko, GwangPyo Springer-Verlag 2018 Environmental science and pollution research inter Vol.25 No.30