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( Hyo Jung Cho ),( Gil Ho Lee ),( So Young Yoon ),( Jin Young Nam ),( Jeong Mi Kim ),( Do Wan Kim ),( Jae Youn Cheong ),( Sung Won Cho ),( Soon Sun Kim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Exosome plays important role in cell-to-cell communication. MicroRNAs in tumor-derived exosome appear to play an important role in cancer progression. The present study explored the differentially expressed microRNA profiles between hepatocellular carcinoma (HCC)-derived exosome (HEX) and normal hepatocyte-derived exosome (NEX) and investigated the target genes for microRNAs in HEX by using network analysis. Methods: Exosomes were isolated from human HCC cell lines (Hep3B and Huh-7) and immortalized normal hepatocyte cell line (THLE-2). Exosomal small RNAs were extracted and differentially expressed exosomal microRNAs between HEX and NEX were screened by using Ilumina Hiseq2000 deep sequencing. Ingenuity Pathway Analysis (IPA) software was used to identify the microRNA regulatory network analysis. Results: A total of 1,830 differentially expressed microRNAs in HEX were identified by deep sequencing. Among them, top 30 up-regulated and top 30 down-regulated exosomal microRNAs were identified in each Huh-7 and Hep3B. By using the differentially expressed microRNA profiles, network analyses were performed. Finally, SMAD3, IFNGR1, and WWP1 were identified as over-expressed hub gene in target network in Hep3B derived exosomal microRNA, while IGF1R, RAC1, and TNF were predicted as over-expressed hub gene in Huh-7 derived exosomal microRNA network. Conclusions: In conclusion, HCC derived exosomal microRNAs could have a central role in HCC progression by acting on the oncogenic pathways. Several key genes for HCC progression were successfully identified by regulatory network analysis of microRNA in HCC derived exosome.
Composite Model Reference Adaptive Control with Parameter Convergence Under Finite Excitation
Cho, Namhoon,Shin, Hyo-Sang,Kim, Youdan,Tsourdos, Antonios Institute of Electrical and Electronics Engineers 2018 IEEE transactions on automatic control Vol.63 No.3
<P>A new parameter estimation method is proposed in the framework of composite model reference adaptive control for improved parameter convergence without persistent excitation. The regressor filtering scheme is adopted to perform the parameter estimation with signals that can be obtained easily. A new framework for residual signal construction is proposed. The incoming data are first accumulated to build the information matrix, and then its quality is evaluated with respect to a chosen measure to select and store the best one. The information matrix is built to have full rank after sufficient but not persistent excitation. In this way, the exponential convergence of both tracking error and parameter estimation error can be guaranteed without persistent oscillation in the external command, which drives the system. Numerical simulations are performed to verify the theoretical findings and to demonstrate the advantages of the proposed adaptation law over the standard direct adaptation law.</P>
Newly developed method for mouse olfactory behavior tests using an automatic video tracking system
Cho, Hyung-Ju,Lee, Yong Hyuk,Kim, Bo Ra,Kim, Hong-Kyung,Chung, Hyo Jin,Park, Sang Chul,Seong, Je Kyung,Yoon, Joo-Heon,Kim, Chang-Hoon Elsevier 2018 Auris, nasus, larynx Vol.45 No.1
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>The mouse is the most popular animal model in olfactory research. Behavior tests with odorants are essential for determining olfactory phenotype. To the best of our knowledge, the mouse olfactory behavior test has not been standardized, making the results vulnerable to inter-observer variation. We sought to develop a new mouse olfactory behavior test assessed by an automatic video tracking system with minimal inter-observer variation.</P> <P><B>Methods</B></P> <P>A video-tracking system was used to automatically track mouse behavior in standard breeding cages with C57BL/6N mice. We tested two odorants (peanut butter for the preference test, 2MB acid for the avoidance test) and distilled water (for a control). Mouse behavior was recorded for 3min and analyzed. For the preference test, investigation time was measured. For the avoidance test, time spent in sectors away from the odorant zone was measured. To confirm our experimental settings, we also evaluated an anosmia mouse model prepared with intranasal administration of ZnSO<SUB>4</SUB>.</P> <P><B>Results</B></P> <P>All strains of mice showed reproducible behavior patterns of preference or avoidance for the odorants. The anosmia mouse model, as expected, failed to show an olfactory ability for preference or avoidance, and this was well-matched by histologic changes caused by the ZnSO<SUB>4</SUB> treatment. The automatic video tracking system successfully tracked and automatically calculated mouse behavior with good reproducibility.</P> <P><B>Conclusion</B></P> <P>Our olfactory behavior test offers a simple and accurate method to evaluate olfactory function in mice. This test can be utilized as a possible standard method to search for features of olfactory phenotypes in mice.</P>
A nationwide study of the incidence rate of herb-induced liver injury in Korea
Cho, Jung-Hyo,Oh, Dal-Seok,Hong, Sang-Hoon,Ko, Heung,Lee, Nam-Hun,Park, Sang-Eun,Han, Chang-Woo,Kim, Seung-Mo,Kim, Young-Chul,Kim, Kang-San,Choi, Chang-Won,Shin, Seon-My,Kim, Ki-Tae,Choi, Hong-Sik,Lee Springer Berlin Heidelberg 2017 Archives of toxicology Vol.91 No.12
<P>Discrepant incidence has been reported regarding the incidence of herb-induced liver injury (HILI). To address the growing worldwide concern of HILI, we evaluated the risk of HILI in a nationwide prospective study. Between April 2013 and January 2016, 1001 inpatients (360 males and 641 females) from 10 tertiary hospitals throughout South Korea were treated with herbal drugs and had their liver enzymes periodically measured. A total of six patients met the criteria for HILI with RUCAM scores ranging from 4 to 7. All these participants were women and developed the hepatocellular type of HILI. One HILI participant met the criteria for <I>Hy</I>’s law; however, none of six cases presented clinical symptoms related to liver injury. This is the first nationwide prospective study that estimated the extent of the incidence of HILI [total: 0.60%, 95% confidence interval (CI) 0.12–1.08; women: 0.95%, 95% CI 0.19–1.68] and described its features in hospitalized participants.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1007/s00204-017-2007-9) contains supplementary material, which is available to authorized users.</P>
Cho, Gyu-bong,Kim, Jae-kwang,Lee, Sang-hoon,Kim, Guk-tae,Noh, Jung-pil,Cho, Kwon-koo,Kim, Ki-won,Nam, Tae-hyun,Ahn, Hyo-jun Elsevier 2017 ELECTROCHIMICA ACTA Vol.224 No.-
<P><B>Abstract</B></P> <P>In this study, two types of patterned (wholly and selectively covered) Si film electrodes containing trench-structured Cu current collectors are proposed for use in thin-film Li-ion batteries. The proposed electrodes are fabricated using a standard laser printer and electrochemical etching. The formation of the trench structure in the Si film electrodes results in improved electrochemical performance compared to conventional continuous Si film electrodes. The wholly covered Si film (WCS) electrode exhibits an 18.5% increase in capacity compared to the continuous Si film electrode. The selectively covered Si film (SCS) electrode, which is covered by Si film only at the bottom of the trenches, exhibits better electrochemical performance than the WCS electrode. An SCS electrode with a trench width of 45μm and trench height of 14.3μm exhibits the best electrochemical properties in terms of efficiency (85% in the first cycle) and cycle performance (60% capacity retention after 100 cycles).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Trench-structured Cu current collector was fabricated using facile method. </LI> <LI> Wholly covered Si film electrode exhibited an increase in capacity. </LI> <LI> Selectively covered Si film electrode exhibited good efficiency and cycleability. </LI> </UL> </P>
Cho, Yong-Jun,Lee, Chi Hern,Kim, Dae Won,Yoo, Ki-Yeon,Eum, Won Sik,Shin, Min Jea,Jo, Hyo Sang,Park, Jinseu,Han, Kyu Hyung,Lee, Keun Wook,Choi, Soo Young Journal of Neurosurgery Publishing Group 2018 Journal of Neurosurgery: Spine Vol.29 No.5
<B>OBJECTIVE</B><P>Adhesion formation is one of the most common complications following laminectomy. The efficiency of antiadhesive agents is required to prevent postsurgical adhesion. Therefore, the authors investigated the effects of silk solution against laminectomy-induced dural adhesion formation in a rat model.</P><B>METHODS</B><P>The authors prepared the silk solution from silkworms. Laminectomies were performed between L3 and L5 in all rats. MediShield was used as a control. The effects of silk solution against laminectomy-induced dural adhesion formation and inflammation were confirmed by histological examination and Western blot analysis.</P><B>RESULTS</B><P>Laminectomy-induced dural adhesion formation was drastically reduced in silk solution-treated rats (Grade 2) compared to vehicle-treated rats (Grade 3). Dural adhesion formation was significantly reduced in rats that received treatment with silk solution or MediShield, which is a known antiadhesion drug. In addition, combined treatment with silk solution and MediShield showed greater reductions in inflammation compared to the silk solution- or MediShield-treated rats (p > 0.05).</P><B>CONCLUSIONS</B><P>In this study, the authors demonstrated that silk solution effectively reduced postlaminectomy dural adhesion formation in rats. Although further studies on the long-term effects are necessary to validate these results, silk solution may potentially serve as an antiadhesion agent in neurological procedures.</P>