림프절 생검으로 진단된 복강내 간질섬유형성 소원형 세포 종양 1예 보고

주명진,이광민,이혜경,정동규 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.5

개의 콜레스테롤 담석 증례

전혜영,박성환,한재익,이숙진,김근형,정동우,나기정 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

약물 유해반응 보고지침 -임상시험의 안전성에 관한 정보 관리지침 -

지동현,김훈교,배진우,손동렬,김인범,전용관,박혜연 大韓臨床藥理學會 1998 臨床藥理學會誌 Vol.6 No.2

足三里의 電鍼刺戟이 흰쥐의 中樞神經系에서 Interleukin-6의 活性에 미치는 影響 - 求心性 體感覺 情報傳達을 中心으로- : 求心性 體感覺 情保傳達을 중심으로

이혜정,신형철,진수희,손양선,윤동학,임사비나 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Objectives : Acupuncture is expected to have somewhat like the efficacy parallel increasing activity of immune system in Western modern medicine. There, already, are many animal researches on activating effect of acupuncture for the immune system in peripheral organs. So, we carried out this experiment to see whether acupuncture has controlling effect on interleukin-6(IL-6) activity in rat's brain. Methods and Results : We had topical application of IL-6(1U-1pg, 10㎕) on brain of rat. It reduced afferent sensory transmission to the primary somatosensory(SI) cortex from periphery. Whereas, electrical stimulation(ES, 2Hz, 1.5V, 15min) of ST36(足三里) with application of IL-6 prominently activated afferent sensory transmission. ES of non-acupoint(proximal tail) with IL-6 showed suppression of afferent transmission. ES of ST36 without IL-6 application also exerted facilitation of afferent transmission to the SI cortex. Conclusions : Electoacupuncture(EA) on ST36 has noticeable influences on modulating activation of IL-6 in central nervous system, which do major role in immune system.

환기정도에 따른 수술실용 신발 종류가 수술실 오염에 미치는 영향 : How to Choose Suitable Shoes?

남경동,정혜선,박영신,원진희,주미자,성화신,이지혜,이병희,조경숙,배재춘 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.1

Background : Various types of protective footwear have been used to minimize bacterial contamination in operating rooms. In recent years, debate has arisen concerning the need for use of such protective footwear. This study was designed to provide useful data about choosing shoes most suitable for the surgical environment. Methods : Between November, 1999 and January, 2000, we performed this experimental study by comparing effect of three types of shoes (i.e., disposable shoescover, operating room-restricted shoes, and ordinary shoes) on bacterial contamination of operating rooms equipped with two different ventilation systems (i.e., high air-change, low air-change) respectively. Data were collected during two-hour sham operations in which subjects and their activities were strictly standardized. Bacterial flora were sampled from the study area floor and air colony-counts were measured. Results : In experiments involving high air-change ventilation system, there was a significant difference of floor contamination between three types of shoes, but no difference of air contamination. Under low air-change system, there was a significant difference of both floor and air contamination between three types of shoes. Conclusion : The results show that protective footwear would be unnecessary in the operating room with high air-change ventilation system, but it is important to choose suitable shoes carefully under low air-change system. Therefore, the use of outdoor shoes can be considered under high air-change system, but it would seem sensible to apply their first use in less bloody operations at the day surgery center or out-patient department to prevent transfer of body fluid into the outside environment.

Panipenem을 포함한 Carbapenem 계열 항생제의 몇 가지 임상균주에 대한 시험관내 항균력

이동건,김명신,강진한,전혜선,최수미,위성헌,김상일,최정현,유진홍,신완식,강문원 대한감염학회 2003 감염과 화학요법 Vol.35 No.2

목적 : Panipenem은 일본에서 개발된 카바페넴계 항생제로 그람양성균, 그람음성균, 혐기균에 이르기까지 광범위한 항균력을 나타낸다고 보고되고 있지만 국내에서 분리되는 균주에 대한 감수성 검사결과는 아직 없다. 본 연구에서는 임상에서 분리된 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae 균주를 대상으로 carbapenem 계열 및 몇 가지 항생제와 감수성을 비교하고, 특히 P. aeruginosa의 경우 carbapenem의 효과를 Muller-Hinton agar (MHA)가 반감시킬 수 있는 특성을 고려하여 감수성에 변화가 있는지 확인하고자 하였다. 방법 : 2002년 3월부터 6월까지 가톨릭대학교 성모병원, 강남성모병원, 성가병원, 성모자애병원, 성빈센트병원에서 분리된 임상균주 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae를 대상으로 하였다. 항생제 감수성 검사는 NCCLS 기준에 따랐고 P. aeruginosa의 경우 MHA 이외에 minimal agar Davis (MAD)를 추가로 사용하였다. 감수성 결과 해석시 panipenem은 imipenem과 같은 기준을 적용하였다. 결과 : E. coli, K. pneumoniae, P. aeruginosa는 각각 100균주였고, S. penumoniae는 134균주였다(PSSP 41, PNSP 93균주). E. coli, K. pneumoniae는 모든 균주가 imipenem, meropenem, panipenem에 대해 감수성을 보였다. P. aeruginosa의 경우 imipenem, meropenem, panipenem, ceftazidime의 MIC_(90)은 각각 16, 8, 32, 64㎍/mL이었다. MHA와 MAD 배지를 이용하여 P. aeruginosa에 대한 항생제 감수성을 비교해 본 결과 imipenem, panipenem의 MIC_(90)가 각각 16, 32㎍/mL에서 8㎍/mL로 감소하였고, imipenem에 비해 panipenem이 더 많이 감소하였다. S. pneumoniae의 경우 PSSP는 imipenem, meropenem, panipenem에 대해 모두 내성이 없었고 PNSP는 imipenem, meropenem, panipenem에 대한 MIC_(90)가 각각 1, 2, 0.25㎍/mL이었다. 결론 : 결론적으로 panipenem은 E. coli, K. pneumoniae에 대해 기존의 카바페넴계 항생제와 유사한 우수한 항균력을 보였다. P. aeruginosa의 경우 ceftazidime, imipenem과 비슷한 항균력을 보였고 아미노산 함량이 적은 배지를 사용함에 따라 MIC가 낮아졌으며 imipenem보다 더 많은 영향을 받았다. S. pneumoniae의 경우 특히 PNSP에서 기존의 카바페넴계 항생제보다 우수한 항균력을 보였다. Panipenem은 주요 그람음성균 및 폐렴연쇄구균 감염에 유용할 것으로 기대된다. Background : Panipenern (PAPM) is a new carbapenern which has an enhanced broad spectrum against both gram-positive and negative organisms. The aim of study was to compare the activities of PAPM with those of imipenern (IMPM), meropenern (MRPM) against several clinical isolates in Korea. Methods : We tested the in vitro antimicrobial activities of PAPM, IMPM, and MRPM against total 300 clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa and 134 Streptococcus pneumoniae collected from 5 different university hospitals. Using NCCLS guidelines, MICs of PAPM, IMPM, MRPM, and/or ceftazidime were determined. Results : All isolates of E. coli and K. pneumoniae were susceptible to PAPM, IMPM and MRPM. MIG_(90) of PAPM, IMPM, and MRPM against P. aeruginosa were 32, 16, and 8 ㎍/mL, respectively. Comparing Mueller-Hinton agar (MHA) and minimal agar Davis (MAD), the MIG_(go) of PAPM and IMPM were reduced from 16 and 32 ㎍/mL to 8 and 8 ㎍/mL, respectively. PAPM was more influenced by MAD than IMPM. All isolates of penicillin susceptible S. pneumoniae showed 0% of resistance to the carbapenems tested. MIC_(90) of PAPM, IMPM and PRPM against penicillin non-susceptible S. pneumoniae were 0.25, 1 and 2 ㎍/mL, respectively. Conclusion : Panipenern could be one of the potentially useful drugs for the treatment of infections caused by E. coli, K. pneumoniae, and S. pneumoniae. We also showed that PAPM had good anti-pseudomonal activity when examined in MAD, the amino acid-limited media. Therefore, it could be useful for the treatment of infections caused by P. aeruginosa.

PPV 공중합체의 합성 및 발광특성

민혜경,이경민,한동제,박성진,오응주 명지대학교 자연과학연구소 1997 자연과학논문집 Vol.16 No.-

Blue light emitting PPV [poly (p-phenylene vinylene)] copolymers soluble in common organic solvents are synthesized and their structural and light emitting properties are studied. Copolymers show π-π* transition in UV-Vis. spectra and their λmax shift to shorter wavelength compare to that of monomers. The PL and absorption spectrum shows the symmetric vibration modes with muror images which means that copolymers are highly, aligned. The band gap energy of these copolymers are ~3.0 eV. By introducing aliphatic hydrocarbon group into polymer main chain, the stability of copolymers is increased and no significant effects of substituents are observed.

비주얼 베이직을 활용한 초등학교 고학년용 통계교육 모형

박동준,강혜진 한국수학교육학회 2000 初等 數學敎育 Vol.4 No.2

제7차 교육과정의 확률과 통계영역의 내용에 맞추어 초등학교 고학년을 위하여 비주얼 베이직으로 프로그램한 통계교육모형을 개발하였다. 10개의 모듈로 구성된 통계교육모형은 초등학생들이 쉽게 사용할 수 있도록 하였고, 모형을 실행시키면 다양한 색깔을 갖는 물체들이 컴퓨터의 화면에 나타나고 마우스의 클릭만으로 동적 기능을 볼 수 있도록 하였다. 통계교육모형의 사용 후 설문조사 결과, 모형의 내용과 특성에 대해 긍정적인 평가가 있었고 초등학생들이 확률과 통계에 높은 흥미와 관심을 나타냈다. 마련된 홈페이지에 모형의 실행파일을 올려놓으면 무료로 다운로드하여 사용할 수 있도록 하였다. Based on the contents of probability and statistics areas in the seventh curriculum, we developed a statistics education model programmed by visual basic for upper grade students of elementary schools. The model that was consisted of ten modules can be easily manipulated by elementary school students. The objects with a variety of colors are presented on P.C. monitor and dynamic functions are performed by clicking a mouse. According to the survey after using the model, students positively evaluated the contents and characteristics and showed great interest in the model. We will upload the execution files of the model on our homepage. One can download and use them free.

선천성 피부 형성부전증 1례 보고

황성진,박혜림,김희석,김동철,김정헌 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.2

Aplasia cutis congenita is a disorder of heterogeneous group characterized by isolate or multiple, localized or widespread areas of skin defect at birth. Since the first report by Cordon in 1767, it has been relatively rarely reported disorder abroad as well as domestically . It has several clinical subtypes classified by the location and pattern of skin absence, the presence of associated malformations and the mode of inheritance with unknown cause. We had a newborn male infant with this disorder clinically diagnosed, who showed wide full thickness skin defects on right knee and medial surface of ankle from birth, that covered with thin yellowish membrane and showed focal ulcerative area. On his familial history, there were four aplasia cutis congenita patients in the patient's mother in his maternal family side through four generations. Patient mother showed wide spread atrophic scarred looking appearance on her right lower leg. Chromosomal analysis revealed as normal male karyotype. The biopsy of lesions showed ulcerated surface and underlying chronic inflammation with total absence of epidermis. He had no associated congenital anomaly. The lesions healed rapidly for four weeks by conservative treatment as moist wound dressing and systemic antibiotic administrations. As a relatively uncommon skin anomaly, one case of aplasia cutis congenita is reported with review of references.

새로운 Baculoviurs 전이벡터를 이용한 Escherichia coli β-galactosidase 유전자의 발현

우수동,김우진,김혜성,진병래,강석권 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

국내에서 분리된 BmNPV를 이용하여 제작된 새로운 전이벡터 pBmKSK1에 외래 유전자로서 E. coli lacZ 유전자를 클로닝하고 재조합 바이러스를 제작하였다. 재조합 바이러스에 대하여 Southern blotting 분석을 실시하여 lacZ 유전자의 존재를 확인하였으며, 재조합 바이러스가 접종된 세포의 SDS-PAGE 및 Western blotting 분석을 통하여 β-galactosidase의 발현을 확인하였다. 재조합 바이러스의 발현효율을 누에 세포주 및 유충에서 비교 조사한 결과, 유충에서 더욱 높은 발현량을 보임을 확인하였다. To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells coninfected with transfer vector pBmKSK1-LacZ and vild type BmNPV genome, and analysed by Southern blotting. The expression of β-galactosidase was characterized by SDS-PAGE, Western blotting and β-galactosidase activity assay. The results showed that the level of expression in silkworn larvae was higher than that of BmN-4 cells.