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김유진,박미경,박이랑,이보람,이혜림,전선미,양난영,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38
The results of this Study are as follows:33.6% of all participants have insomnia; 22.5% of those who have insomnia are DIS(difficulty in initiating sleep), 17.3% are DMS(difficulty returning to sleep once awakened) and 7.0% awakened too early. 3 4.8% experience sleepiness during daytime. Type 1, experiencing insomnia and sleepi ness during daytime together, is 12.0%, Type 2, with insomnia only, is 21.6%, Type 3, with sleepiness during daytime only, is 22.8% and 43.5% experience no sleeping disturbances. After studying only those with 3 types of sleeping disturbances, it is found that the most common cause of such disturbance is stress 88.4%, anxiety 56.0%, no apparent reason 33.8%, anxiety/fear/terror 29.3%, hurry 23.6%, alcohol/caffeine 16.9%, bedroom tem perature 11.1%, urination during nighttime and persons living together 10.7%, noise from inside 8.9%, illumination 8.0%, and pain/itch 5.8%. The one group revealed significant differences in residential environment(p=0.003). Sex, age, education level, medicine, monthly earning revealed no meaningful differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), whether taking enough sleep(p=0.029), whether taking regular sleep(p=0.005) showed significant differences depending on whether or not having insomnia, and mean duration of sleep time, time to sleep, time of rising, whether taking naps did not reveal significant differences. Of sleep behavior, time to sleep(p=0.000), whether taking naps(p=0.000), indicated significant differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), and whether taking enough sleep(p=0.000), time of going to bed (p=0.002), whether or not taking nap(p=0.000), whether or not taking regular sleep(p=0.010) indicated significant differences among the sleeping disturbance types.
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레티닉스 알고리즘과 주성분 분석 방법을 이용한 실시간 피부 영역 검출
길혜진,이준호 成均館大學校 科學技術硏究所 2002 論文集 Vol.53 No.2
본 논문은 인접한 두 픽셀의 비율을 계산하는 방법인 레티닉스 알고리즘을 전처리 과정으로 사용하여 조명 변화로 생기는 효과를 우선적으로 제거하였다. 또한 주성분 분석 방법을 사용하여 색 불변성을 유지할 수 있었으며, 온라인 학습 방법을 사용하여 학습에 소요되는 시간을 현저하게 줄이고 실시간 시스템에도 바로 적용할 수 있도록 하였다. 그 결과 조명이 변화하는 환경에서 적은 양의 학습 데이터를 이용하여 강인한 피부색 검출 성능을 보이는 시스템을 구성할 수 있었다.
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곽수진,이윤정,정혜선 韓國學校保健學會 2009 韓國學校保健學會誌 Vol.22 No.2
Purpose: By analyzing factors related to suicide ideation of Korean middle and high school students. the purpose of this study was to multi-dimensionally understand suicide ideation of Korean middle and high school students and arrange the plans to prevent suicide. Methods: This study used and analyzed the data on 'The 3rd (Year2007) Korea Youth Health Risk Behavior We based Survey (KYHRBWS)' conducted by the Korea Centers for Disease Control and Prevention. In this study we analyzed 74,698 respondents in total. SPSS 12.0 Program was used to analyze the survey. Descriptive statistics, Chi-Square Test and multivariate logistic regression were also used as research methods. Results: 23.8% of the Korean middle and high school students had suicide ideation during the last one year. Girls had higher possibility of suicide ideation by 1.51 times compared to boys. Students with poor academic achievement had more suicide ideation. In terms of health characteristics. students who perceived. themselves as not healthy. and students with short weekday sleeping hours. more drinking experience and more smoking experience had more suicide ideation than others. Furthermore. students who perceived themselves as overweight or obese had more suicide ideation than other students. In terms of mental characteristics, students with high level of stress. low level of happiness and depression had more suicide ideation. Conclusion : As a resuit of this study. we fa found out that the factor that had the biggest influence on suicide ideation of Korean middle and high school students was depression. Consequently, active supports and policies to decrease suicide ideation of Korean middle and high school students are necessary. Therefore, active interviews' education for suicide prevention, and periodical screening inspection should be introduced in schools
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정순재,이용재,김혜진,이영병,이용문 東亞大學校附設 農業生命科學硏究所 1999 農業生命資援硏究 Vol.8 No.1
'부유' 단감의 접목시기별 묘목의 생장과 토양 깊이별 접목 후의 생장을 알고자 시험한 결과는 다음과 같다. 대목싹은 접목시기가 늦을수록 많은 경향을 나타내었다. 접목 활착율은 시기별로 보아 5월 21일까지는 차이가 없었고 5월 30일은 7%정도 약간 낮았다. 접목일수와 접목활착일의 차이는 접목시기가 늦을수록 단축되었다. 접목 일자가 빠른 4월 25일, 5월 1일 접목구의 신초장은 상지보다 하지가 5.17cm 5.33cm가 더 길었다. 그리고 그 외는 대부분 상지가 하지보다 좋은 현상을 나타내었다. 시기별 접목에 관계하지 않고 근의 직경이 큰 구가 신초 신장이 큰 효과를 나타내었다. 토양의 깊이별 묘목 생장은 25cm 구가 신초의 직경이 크고 신초장이 가장 길었다. The study was conducted to assess the growth and development of 'Fuyu' grafted nursery plant according to various grafting time and soil depth. The earlier grafting time was taken, the more shoots were got rid. The cv. 'Fuyu' was grafted on the rootstock of Kyungsan Bansi cv. After grafting, graft-take rates was not showed any difference until on may 21 however it was little bit low on may 30. The later grafting time was taken, the more graft-take days were shortened. Bottom shoot length of scion was 5.17cm longer than top's on April 25 and 5.33cm longer than top's on April 1. Top shoot length was longer than bottom shoot length most of other days. The growth and development of persimmon seeding was related to root weight. The growth and development of persimmon tree according to the soil depth showed beter results in the 25cm treatment than any other treatments.
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Development of a Test Method for the Evaluation of DNA Damage in Mouse Spermatogonial Stem Cells
Hye Lyun Jeon,Jung-Sun Yi,Tae Sung Kim,Youkyung Oh,Hye Jeong Lee,Minseong Lee,Jin Seok Bang,Kinarm Ko,Il Young Ahn,Kyungyuk Ko,Joohwan Kim,Hye-Kyung Park,Jong Kwon Lee,Soo Jung Sohn 한국독성학회 2017 Toxicological Research Vol.33 No.2
Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving mSSCs by comparing their results with those of in vivo tests using C57BL/6 mice by gavage. We selected hydroxyurea (HU), which is known to chemically induce male reproductive toxicity. The 50% inhibitory concentration (IC50) value of HU was 0.9 mM, as determined by the MTT assay. In the in vitro comet assay, % tail DNA and Olive tail moment (OTM) after HU administration increased significantly, compared to the control. Annexin V, PI staining and TUNEL assays showed that HU caused apoptosis in mSSCs. In order to compare in vitro tests with in vivo tests, the same substances were administered to male C57BL/6 mice. Reproductive toxicity was observed at 25, 50, 100, and 200 mg/kg/day as measured by clinical measures of reduction in sperm motility and testicular weight. The comet assay, DCFH-DA assay, H&E staining, and TUNEL assay were also performed. The results of the test with C57BL/6 mice were similar to those with mSSCs for HU treatment. Finally, linear regression analysis showed a strong positive correlation between results of in vitro tests and those of in vivo. In conclusion, the present study is the first to demonstrate the effect of HU-induced DNA damage, ROS formation, and apoptosis in mSSCs. Further, the results of the current study suggest that mSSCs could be a useful model to predict male reproductive toxicity.
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Hye Jin Hwang,Hye Young Jin,Eun Jung Chung,Soo Yeon Kim,Yi Sub Kwak 한국식품영양과학회 2008 Preventive Nutrition and Food Science Vol.13 No.4
The objective of this study was to investigate the effects of a nutritional education and physical exercise program on obese children (47 boys and 36 girls). The nutritional education and physical exercise program was provided for 6 weeks and dietary habits, nutrient intake and hematological profiles were measured before and after the program. The BMI (body mass index, ㎏/㎡) in male children was significantly reduced to 24.58±2.81 from 25.89±3.27 (p<0.01) and it was significantly reduced to 24.29±3.51 from 25.21±3.59 in female children after the completion of nutritional education and exercise program. Body fat in male children was remarkably reduced to 32.69±7.74% after education from 37.38±9.21% (p<0.001). After the program total cholesterol concentration in male children was reduced to 176.48±29.10 ㎎/dL from 196.03±29.10 ㎎/dL, whereas there was no significant difference in females before and after the nutritional education and exercise program. The grasping power of male children (p<0.05) and backmuscle strength of male (p<0.05) and female children (p<0.001) were significantly increased after the exercise program. The nutrient intakes that were different before and after the nutrition education were calories (p<0.05) and carbohydrate (p<0.05) in male children, and a significant increase in intake of vitamin A (p<0.05) was found in female children. There was a significant difference in meal time regularity before and after program. The findings of this study showed that there was a significant decrease in both body fat and weight, in addition to normalization of hematological profiles after the completion of nutrition education and physical exercise programs.
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Hwang, Hye-Jin,Jin, Hye-Young,Chung, Eun-Jung,Kim, Soo-Yeon,Kwak, Yi-Sub The Korean Society of Food Science and Nutrition 2008 Preventive Nutrition and Food Science Vol.13 No.4
The objective of this study was to investigate the effects of a nutritional education and physical exercise program on obese children (47 boys and 36 girls). The nutritional education and physical exercise program was provided for 6 weeks and dietary habits, nutrient intake and hematological profiles were measured before and after the program. The BMI (body mass index, $kg/m^2$) in male children was significantly reduced to $24.58{\pm}2.81$ from $25.89{\pm}3.27$ (p<0.01) and it was significantly reduced to $24.29{\pm}3.51$ from $25.21{\pm}3.59$ in female children after the completion of nutritional education and exercise program. Body fat in male children was remarkably reduced to $32.69{\pm}7.74%$ after education from $37.38{\pm}9.21%$ (p<0.001). After the program total cholesterol concentration in male children was reduced to $176.48{\pm}29.10\;mg/dL$ from $196.03{\pm}29.10\;mg/dL$, whereas there was no significant difference in females before and after the nutritional education and exercise program. The grasping power of male children (p<0.05) and backmuscle strength of male (p<0.05) and female children (p<0.001) were significantly increased after the exercise program. The nutrient intakes that were different before and after the nutrition education were calories (p<0.05) and carbohydrate (p<0.05) in male children, and a significant increase in intake of vitamin A (p<0.05) was found in female children. There was a significant difference in meal time regularity before and after program. The findings of this study showed that there was a significant decrease in both body fat and weight, in addition to normalization of hematological profiles after the completion of nutrition education and physical exercise programs.
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