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병원근무자에서 홍역,풍진 및 수두 항체양성률에 관한 연구
최강원,김성민,오명돈,이환종,오향순,최희정,김남중,신형식 대한감염학회 1997 감염 Vol.29 No.1
목적: 홍역, 풍진, 및 수두의 병원감염 발생위험도를 평가하고 병원근무자 예방접종지침을 고안하는데 필요한 기초자료를 얻고자, 홍역, 풍진, 및 수두에 대한 병원근무자의 항체양성률을 조사하였다. 방법: 1995년 3월 서울대학교병원 산부인과 병동과 서울대학교 어린이병원에 근무하고 있는 여성을 대상으로 홍역, 풍진, 및 수두의 감염 과거력과 예방접종 과기력을 설문조사하였다. 정맥혈을 채혈하여 효소결합면역흡착검시(Enzyme linked immunosorbent assay, Behring ??? IgG kit)로 IgG 항체를 측정하였다. 결과: 홍역, 풍진, 및 수두에 대한 항체양성률은 각각 95.6%, 87.9%, 96.2% 이었다. 연령, 감염 과거력, 예방접종 과거력에 따른 항체양성률의 차이는 유의하지 않았다. 결론: 저자 등의 연구결과는 홍역과 수두, 특히 풍진의 병원감염 위험도가 높음을 시사하며, 각 개인의 건강과 전염병의 전파를 막기 위하여 병원근무자 예방접종지침이 필요하다. Background: To assess the risk of hospital outbreak and to guide immunization policy, seroprevalences of antibody to measles, rubella, and varicella-zoster were determined in hospital personnel. Methods: A cross-sectional study of 290 workers in Seoul National University Hospital was conducted in June 1995. IgG antibody status was determined with commercially available enzyme linked immunosorbent assay(Behring ??? IgG kit). Results: Seropositive rates of measles, rubella and varicella-zoster were 95.6%, 87.9% and 96.2%, respectively. There was no statistical difference in seropositive rate according to historical information. Conclusion: These results suggest potential for hospital outbreak of measles an varicella-zoster, especially of rubella. Immunization policy will be needed in hospital personnel.
Kwon, Hyang-Mi,Choi, Yean-Jung,Choi, Jung-Suk,Kang, Sang-Wook,Bae, Ji-Young,Kang, Il-Jun,Jun, Jong-Gab,Lee, Sang-Soo,Lim, Soon Sung,Kang, Young-Hee The Society 2007 Experimental biology and medicine Vol.232 No.2
<P>Numerous polyphenolic compounds have been found to inhibit adhesion and migration of leukocytes to sites of inflammation that are partly regulated by the expression of cell adhesion molecules (CAM) such as vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and platelet endothelial cell adhesion molecule-1 (PECAM-1). Licorice root extracts have been used in traditional Chinese, Tibetan, and Indian medicine for the treatment of pulmonary diseases and inflammatory processes. Expression of CAM proteins was examined in human umbilical vein endothelial cells (HUVEC) treated with a licorice component (isoliquiritigenin, 18beta-glycyrrhetinic acid, glycyrrhizin, formononetin, or ononin) and exposed to TNF-alpha. The involvement of NF-kappaB in the transcriptional control of CAM proteins was assessed by degradation of IkappaBalpha and nuclear translocation of NF-kappaB using Western blotting techniques and immunocytochemical staining. At nontoxic > or =10 microM, isoliquiritigenin blocked the induction of VCAM-1 and E-selectin on activated HUVEC and markedly interfered with THP-1 monocyte adhesion to TNF-alpha-activated endothelial cells. Isoliquiritigenin abolished TNF-alpha-induced mRNA accumulation of VCAM-1 and E-selectin. Additionally, immunocytochemical staining revealed that isoliquiritigenin attenuated PECAM-1 expression induced by TNF-alpha. In contrast, other components recognized in licorice, 18beta-glycyrrhetinic acid, glycyrrhizin, formononetin, and ononin did not down-regulate the expression of VCAM-1 and/or PECAM-1 activated by TNF-alpha, implying that these components are inactive in modulating adhesion of leukocytes to stimulated endothelial cells. Isoliquiritigenin downregulated CAM proteins in TNF-alpha-activated HUVEC at the transcriptional levels by blocking degradation of IkappaBalpha and nuclear translocation of NF-kappaB. These results demonstrate that the induction blockade of VCAM-1 and E-selectin by isoliquiritigenin was directly mediated by its interference with the CAM mRNA transcription through NF-kappaB-dependent mechanisms under inflammatory conditions.</P>
Identification of Novel Genes with Proapoptotic Activity
Kang Eun-Ju,Kim Jeong-Min,Kim Na-Young,Park Kyung-Mi,Park Seong-Min,Kim Nam-Soon,Yoo Hyang-Sook,Yeom Young-Il,Kim Soo-Jung Korea Genome Organization 2006 Genomics & informatics Vol.4 No.2
In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypo-thetical genes with proapoptotic activity.
Revisiting Domestication to Revitalize Crop Improvement: The Florigen Revolution
( Soon Ju Park ),( Young Koung Lee ),( Min Sung Kang ),( Jong Hyang Bae ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.4
Most flowering plants have evolved to coordinate proper floral transition in shoot apical meristems at an appropriate time during development, referred to as “flowering time.” In domesticated crops, shoot life time has been manipulated to enhance productivity via synchronizing flowering time to the local environment, thereby creating a balance between vegetative and reproductive plant architecture during the year. Rice, a typical single transitional crop, has acquired balance between prolonged tiller growth and a relatively short reproductive phase on individual shoot, resulting in increasing yield. Crops with sympodial growth, such as tomato, which undergo multiple floral transitions on the main shoot, have been artificially selected for the determination of shoot growth by investigating the dosage sensitivity of florigen activation controlling flowering time on individual shoots. Here, we summarize recent genetic-molecular studies on tomato, which have been subject to genetic manipulation of flowering time in an effort to optimize seed productivity. We also review advanced genetic approaches as potential new tools for the enhancement of crop yield by manipulating flowering time via the dosage effect of florigen.
Kwon Hyang-Mi,Lim Soon Sung,Choi Yean-Jung,Jeong Yu-Jin,Kang Sang-Wook,Bae Ji-Young,Kang Young-Hee The Korean Nutrition Society 2005 Nutritional Sciences Vol.8 No.3
Numerous natural herbal compounds have been reported to inhibit adhesion and migration of leukocytes to the site of inflammation Licorice extracts, which have been widely used in traditional Chinese medicinal preparation, possess various pharmacological effects. Isoliquiritigenin, a biogenetic precursor of flavonoids with various pharmacological effects, is a natural pigment present in licorice. We attempted to explore whether licorice extracts and isoliquiritigenin mitigate monocyte adhesion to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-activated human umbilical vein endothelial cells (HUVEC). In addition, it was tested whether the inhibition of monocyte adhesion to the activated HUVEC accompanied a reduction in vascular cell adhesion molecule-l expression(VCAM-l). Dry-roasted licorice extracts in methylene chloride but not in ethanol markedly interfered with THP-l monocyte adhesion to INF-$\alpha$-activated endothelial cells. licochalcone A compound isolated from licorice extract in methylene chloride appeared to modestly inhibit the interaction of THP-l monocytes and activated endothelium. In addition, isoliquiritigenin abolished the monocyte adhesion with attenuating VCAM-l protein expression on HUVEC induced by INF-$\alpha$. These results demonstrated that non-polar components from dry-roasted licorice extracts containing licochalcone A as well as isoliquiritigenin were active in blocking monocyte adhesion to cytokine-activated endothelimn, which appeared to be mediated most likely through the inhibition of VCAM-l expression on HUVEC. Therefore, licorice may hamper initial inflammatory events on the vascular endothelium involving induction of endothelial cell adhesion molecules.