Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

a-TN-LCD의 전기광학특성에 표면액정배향이 미치는 영향

황율연,이창훈,서대식,이보호 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.2

The characteristics of the response time and the viewing angle on twisted nematic(TN)-liquid crystal display(LCD) and amorphous (a)-TN-LCD without rubbing were investigated. To measure the Tansmission-Voltage, response time, and viewing angle characteristics, we prepared three kinds of LC cells and then studied the surface liquid crystal alignment effect. It was found that the response time on a-TN-LCD was fast compared to the TN-LCD, and the weak anchoring strength was attributed to the fast response time on a-TN-LCD. Also, we obtained the wide viewing angle characteristics on-a-LCD. We suggest that the liquid crystal alignment effect on LCD device is very important for electro-optical characteristics.

펄스정전응력법(PEA)을 이용한 고분자 재료애 따른 공간전하분포와 전도전류 특성

황보승 호남대학교 1999 호남대학교 학술논문집 Vol.20 No.2

The purpose of this paper is to evaluate the effects of space charge on electrical characteristics in polymer insulators, which has been studied by many researchers in the world recently. In this paper, we have manufactured new measurement system which measured simultaneously both space charge distribution and conduction current. By using this system, we have analyzed the effects of space charge on conduction characteristics of polymer insulators such as XLPE and WTR-XLPE under DC voltage. We could find that the characteristics of polymer insulators have good correlation with space charge distribution in polymer insulators.

부산지역 산성강우의 장기적인 화학적 성상의 변화

황성욱,전보경,김찬훈,서정민,최금찬 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

It is important to know the impacts of acid rain because of soil acidification, reduced biological productivity. Many researchers were interested in regional acid problems, and former research were focued in short-term trends of acid rain. It is not easy to evaluate acid rain problems because of many parameters relating to meteorological factors, which are wind, temperature, humidity and other factors. Rainfall sampling for this study was done from September 1933 to December 2000 in Dong-A University, Busan. All Samples were collected in separately to early fraction and succeeding fraction. Also, the samples were measured for pH, and EC, cations and anions were analysed by ion Chromatography.

푸쉬코 메카니즘 언어학 : 원리,방법,적용에 관한 시론 le principe, la me'thode et des applications

황보순 慶北大學校 語學硏究所 1989 語文硏究 Vol.14 No.-

The Anglo-Saxon Chronicle의 어순연구 : Parker 및 Peterborough본을 중심으로

황보근 대구대학교 인문과학연구소 1988 人文科學硏究 Vol.6 No.-

The main purpose of this paper is to describe synchronic word-order types of the Parker and the Peterborough manuscripts of the Anglo-Saxon Chronicle and to explain diachronic word-order changes. The manuscripts were divided, according not to the syntactic characteristics of the sentences analyzed but to the compilers, into four sections: (Ⅰ) 878∼891, (Ⅱ) 925∼955, 971∼1001, (Ⅲ)1122∼1131 and (Ⅳ) 1132∼1154. The sentences in each entry year were analyzed and counted on the basis of three clause types: main, subordinate and relative. The number of clause types in the sections of the ASC is as follows: Ⅰ Ⅱ Ⅲ Ⅳ Main Clauses 113 65 329 249 Subordinate Clauses 13 7 109 81 Relative Clauses 28 7 66 28 Description of the word-order types led to the following conclusions: 1) The dominant word-order types in the main clause are SVX, SXV and X°VS. The frequency of SVX constantly increases from Section Ⅱ through Section Ⅳ. This type tends to have the topical or the semi-topical subject and to be used as the new information sentence conjoined with the preceding X°VS type. The SXV has the topical nominal or pronominal subject, and the pre-verbal X is mostly pronominal. The high frequency of X°VS in Section Ⅰ is due to its use as "the new event order" though it has the topical subject. The decrease in both SXV and X°VS is relatively constant. 2) In the subordinate and relative clauses SXV, SVX are the two dominant orders in Section Ⅰ. But from Section Ⅱ though Section Ⅳ the decrease in SXV is constant, and is coincident with the decrease in the main clause. The reason is to be explained by the hyphothesis that the order of elements in dependent clauses will be changed by analogy to the order of elements in independent clauses.

고대영어의 수동구조

黃保根 대구대학교 인문과학연구소 1986 人文科學硏究 Vol.4 No.-

A structural description of passive constructions in Old English is the primary purpose of this paper. In other words, it can fairy be said to be the preliminary stage for further studies on syntactic changes in English, since diachrony must be studied on the basis of patterned synchronic data. The structural description of passives in Old English, unlike that in Modern English, is not an easy task. Major obstacles are: passives in OE have few corresponding actives, and the analysis performed only on literature-written language-not on spoken one. However, the following can be classified, as the three major patterns of OE passive constructions, by the analysis of them in Anglo-Saxon Chronicles, Alfred’s Pastoral Care, and several primary OE texts available: 1. NP hatan NP beon/wesan NP 2. NP{ weorpan }+PP (Prep) {AP} (Prep+NP) beon/wesan 3. NP weorpan +PP NP (Prep +NP) Except for the Tppe 1, the passive auxiliaries used in OE are beon, wesan and weorpan, and their semantic functions are different in the early OE period. Later they came to be used interchangibly as the original meaning of weorpan disappeared. The identification of the semantic functions of prepositions in agentive adjuncts is grueling. The reason is the agentive adjuncts can be viewed as ‘principle agents’, ‘medium’, ‘means’, ‘cause’, ‘reason’, or ‘instrumentality’according to modern interpretation. The preposition used before agent NPs in Oe are : at, by/be, fram/from, mid, of, purh, etc.

경제적 형광등 교체를 위한 표시장치 개발에 대한 연구

황보승 호남대학교 산업기술연구소 1999 산업기술연구논문집 Vol.7 No.-

본 논문에서는 현재까지 광원의 주류를 이루고 있는 형광등에 의한 불필요한 전력소모를 억제하고 전기요금을 절약하기 위한 최적의 형광등 교체주기 표시에 대한 연구를 수행하였다. 이를 위하여 본 연구에서는 시간경과에 따른 형광등 노화에 의한 소모전력의 증가와 그에 따른 경제성을 분석하였으며, 이를 바탕으로 최적의 교환시기가 되면 자동으로 적색 LED가 소등(또는 점등)되는 장치를 설계 및 개발하였다. 또한 P-spice를 통한 회로 해석과 실험을 통하여 개발된 장치의 정상적인 동작을 확인하였다. In this paper, in order to restrict the power consumption and save electric charges due to the fluorescent lamp which is still main lighting source, we have carried out the studies on the indicating the optimal replacement time of it. For these purposes, we have evaluated the increment of power consumption of the fluorescent lamp and its economical efficiency due to degradation according to elapsed time. On the basis of the evaluation, we have designed and developed the system that turned off(or on) the red LED at the optimal replacement time. We also verified correct operations of the developed system through the circuit simulation by P-spice and experiments.

佛語의 强勢後置傾向 硏究

皇甫淳 慶北大學校 1983 論文集 Vol.35 No.-

공주지역사회 체육의 진흥방안

황보연 공주교육대학교 초등연구원 1974 公州敎大論叢 Vol.11 No.-

.