Polyozellus multiplex가 생산하는 지질과산화 저해물질

황지숙,송경식,김양섭,석순자,이태호,유익동 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

담자균류로부터 지질과산화 저해물질을 탐색하던 중 강원도 오대산 지역에 자생하는 버섯의 한 종류로 부터, Sparague-Dawley계 rat의 간 mirosome 분획의 지질과산화를 강하게 억제하는 물질이 있음을 발견하였다. 이 버섯의 미생물학적 특성을 관찰한 결과 민주름버섯목, 굴뚝버섯과에 속하는 까치버섯(Polyzellus multiplex)으로 동정하였으며 본 버섯의 자실체 methanol 추출물을 benzene, ethylacetate로 순차적으로 추출하고 각종 chromatography를 이용하여 활성물질을 정제한 결과, ethylacetate층으로 부터 황산화활성을 나타내는 PM1을 benzene층으로 부터 지방산 계열의 PM2와 PM3 화합물을 순수하게 분리 정제하였다. PM1은 정색반응 및 각종 기기분석 결과 thelephoric acid계의 화합물로 동정되었으며 PM2 및 PM3는 각각 linoleic acid와 oleic acid로 동정되었다. 지질과산화 활성물질로 분리정제한 PM1 화합물의 IC_50 값은 1.96 ppm이었으며 LD_50 값은 500 mg/Kg 이상이었다. In the course of screening lipid peroxidation inhibitor from basidiomycetes, a mushroom, which was collected at O-Dae mountain in Kangweon-Do, was found to have active compound. The mushroom was identified as Polyzellus multiplex, which belongs to Aphylloporalles Thelephoraceae, on the basis of macroscopic and microscopic characteristics of the fruiting body. The methanol extract of fruiting body was extracted with benzene and ethylacetate, sequentially. By using various kinds of chromatographies, PM1, and PM2 and PM3, were purified from the ethylacetate extract and the benzene extract, respectively. Color reaction and analyses of IR, UV, and NMR spectra indicated that PM1 was a derivative of thelephoric acid, and PM2 and PM3 were linoleic acid and oleic acid, respectively. IC_50 of PM1 for inhibition of lipid peroxidation was 1.96 ppm and LD_50 was 500 mg/kg.

순환기 내과로 자문의뢰된 환자의 임상상

황진용,최지용,손상균,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.4

목적 : 순환기내과 영역은 많은 과로부터 자문의뢰를 받는 영역으로 알려져 있다. 특히 최근 우리나라의 순환기질환의 유형이 변화되고 입원환자가 고령화되면서 동반된 심질환의 유병율도 증가하고있다. 이에 저자들은 타과로 입원하여 순환기내과로 자문의뢰되는 환자의 임상상과 3차병원인 경북대학교병원에서의 순환기내과자문의 유용성 등을 평가하여 향후 순환기질병에 대한 협의진료에 도움을 주고자 본 연구를 시행하였다. 방법 : 1995년 3월부터 순환기내과로 공식자문의뢰된 첫 100명을 대상으로 전향적으로 연령, 의뢰이유, 최종진단명, 수술과의 관련성 여부, 순환기내과의 최종조치, 타과의 순응도 등을 조사하였다. 결과 : 1) 자문을 의뢰한 환자는 평균 58±16(15-89, 중앙치 61)세였고 60세이상의 환자가 57%로 고령의 환자가 많았다. 2) 내과계열 25%, 외과계열 75%로 외과계열의 자문이 많았고 일반외과가 26%로 가장 많았고 내과의 다른 분과가 12%, 신경외과 12%, 정형외과 11%, 흉부외과 11%, 신경과 11% 등의 순이었다. 외과계열중 수술을 시행한 경우는 58례로 이중 응급수술이 13례였다. 술전 위험도 평가를 위한 자문이 38례에서 있었는데 순환기적인 문제로 수술을 받지 못한 경우는 2례(1례는 허혈성 심질환, 1례는 심낭질환)이었다. 이때 응급수술을 제외한 입원일과 술전 자문일파의 간격은 평균 3±7일(입원일-42일)이고 술전 자문일과 수술일과의 간격은 평균 6±4일(1-16일)이었다. 3) 타과의 의뢰이유는 심전도 이상이 36례, 높게 측정된 혈압이 20례, 순환기질환의 과거력 17례, 환자의 증상 때문인 경우가 10례였고 심전도의 이상중에는 부정맥이 18례, 이상 Q파 6례, ST절 이상이 5례, 각차단이 3례의 순이었다. 순환기과거력 중에는 허혈성 심질환이 6례, 현재 혈압은 정상이나 고혈압의 과거력이 있는 경우가 4례 순이었고 환자의 증상은 흉통 6례, 호흡곤란이 3례, 기절이 1례였다. 4) 자문후 최종진단이 가능했던 91례를 진단별(총 97 진단명)로 나누어 보면 고혈압이 28례, 부정맥이 16례, 허혈성 심질환이 14례, 판막질환이 11례, 순환기질환이 아닌경우가 19래였다. 이들중 추적진료가 필요하다고 응답한 경우가 57례였으나 순환기내과 외래에 1번이상 추적진료한 경우는 32례(56%)로 환자의 순응도는 낮았다. 결론 : 순환기내과의 자문의뢰는 고령자에게 많았고 수술과 관련된 경우가 많았으며 심전도의 이상이 의뢰이유로 가장 많았으며 진단별로는 고혈압과 부정맥이 가장 많았다. 그리고 순환기적인문제로 수술을 받지 못한 경우는 드물었고 자문후 지속적인 추적진료를 위해 환자의 교육이 필요하겠다. Objective : Clinical characteristics of the patients with cardiovascular diseases in the general hospital have been changed and prevalance of co-existing cardiovascular disease in the patients who were admitted for non-cardiac diseases has been increased remarkably as the proportion of elderly patients increased rapidly in Korea. We evaluated clinical features of the patients consulted for cardiological evaluation and effectiveness of the consultation in the setting of tertiary general hospital, Kyungpook University Hospital. Method : One hundred patients were studied between January 1995 and January 1996. They were evaluated for the reason of consultation, final diagnosis, final recommendation from cardiologists and its impact on the management of the patients, and compliance of the consulting departments. Results : 1) The ages ranged between 15 and 89 (mean 58±16, median 61) years, 57 patients were male and 43 female. Fifty seven patients were older than 60 years. 2) Consultations from surgical parts were 75 patients. Consultation from general surgery was most frequent (26%), followed by other divisions of internal medicine (12%), neurosurgery (12%) and orthopedic surgery (11%), chest surgery (11%), neurology (11%), obstetrics and gynecology (8%), urology (4%), ophthalmology (2%), dermatology (2%), ENT (1%). Among 75 patients from surgical parts, operation was done on 58 patients, including 13 emergency cases. Preoperative cardiac risk evaluation was the reason of consultation in thirty eight patients, of which operation could not be done in only 2 patients (one had severe ischemic heart disease, the other large pericardial disease). Interval between consultation day and operation day was 6±4 days (1-6 days). 3) Reasons for cardiac consultation were electrocardiographic (ECG) abnormalities in 36 patients, high blood pressure in 20 patients, previous cardiovascular diseases in 17 patients, symptoms of the patients in 10 patients. Among EGG abnormalities, arrhythmias was the reason in 18 patients, abnormal Q waves in 6 patients, ST-T segment abnormalities in 5 patients, bundle branch block in 3 patients. Among previous cardiovascular disease, presence of ischemic heart disease was the reason in 6 patients, previous hypertension in 4 patients. Chest pain was the reason of the consultation in 6 patients, dyspnea in 3 patients, syncope in 1 patient. 4) Definite diagnoses could be made in 91 patients with 96 diagnoses which were hypertension and its complications in 28, arrhythmia with underlying disease in 16, ischemic heart disease in 14. valvular heart disease in 11, non-cardiovascular disease in 19. Cardiological follow up was recommended in 57 patients. However, 25 patients (44%) were lost to follow-up. Conclusion : Cardiological consultation was frequent in the elderly patients. Most frequent reason for consultation was EGG abnormalities. Hypertension or arrhythmia was most common final diagnosis. Inoperability due to cardiovascular problems were rare.

환경가치로 살펴본 총요소생산성의 비교에 관한 연구 : 한ㆍ미 제지산업을 중심으로

황호종,지용선 龍仁大學校 1999 용인대학교 논문집 Vol.17 No.-

Environmentalism is the study of the flows of materials and energy in industrial and consumer activities, of the effects, of these flows on the environment, and of the influences of economic, political, regulatory, and social factors on the flow, use, and transformation of resources. Productivity, which measures the technological and organizational efficiency with which forms transform materials and labor into products, is one of the competitive factors. Managers take a productivity growth seriously because it is the message that their firm's ability to compete with others and to improve profitability and market share is improving. But, in pollution-intensive industries, they insist their views that the environmental protection reduces productivity - no matter how treat the damage that is prevented-and therefore a traditional productivity growth fundamentally misrepresents the industrial process. The main reason is that we now take into account only pollution abatement costs. This study outlined a better approach developed by WRI(World Resource Institute), and reexamined the record of total factor productivity growth, Core studies of the pulp and paper sectors of the U.S and Korea illustrate how this approach influences the record of total productivity growth. The revised estimates of productivity growth are in general higher than the conventional ones both in the U.S and in Korea. However, compared to the results for the U.S, the adjustments of Korea are relatively small. It means that the pulp and paper industry of Korea produced much pollution rather that of the U.S did, That is, the pulp and paper industry of Korea lost the eco-efficiency gains.

중소유통업의 조직화·협업화·전문화 방안에 관한 연구

황호종,지형선 용인대학교 산업경영연구소 1998 산업경영논총 Vol.3 No.-

The purpose of this study is to analyze the problems of domestic retailers as well as to propose the alternatives to increase competitiveness of retailers, The retail channel structure is characterized on its high density and small business, which lead to low productivity, 1ow sales, and low morale of management. To solve these problems, small retailers should try to systematize organization by central buying system, point of sale, electronic ordering system and so on. Furthermore, they should cooperate with regional retailers by establishing central institution. Finally, they focus their business on specialization rather than generalization.

중소기업의 정보화 진단에 관한 연구

황호종,지용선 용인대학교 산업경영연구소 1999 산업경영논총 Vol.5 No.-

Small and Medium sized Enterprises(SMEs) have not yet explored various business strategies to cope with the epochal turns in business environment characterized by the so-called paradigm shift, that is, the recent trend toward open market and deregulation accompanied by changing of political and economic orders, One of the most remarkable strategies in these movements is the power on securing competitive edge in information infrastructure where a relatively low level of information exists. Since information is valuable resources and contributed to enhance competitive edge for enterpises, most of enterprises are investing a large amount of money to develop information technology. However, SMEs are having difficulty in enhancing information infrastructure due to a shortage of fund, technical experts as well as low level of information mind of a chief executive officer and employees. In order to improve information infrastructure of SMEs, we need a methodology for evaluating current information infrastructure and providing the direction of information infrastructure and providing the direction of information planning based on the evaluation. Especially business environment of Korean SMEs are so much different from other leading countries, therefore we need as evaluation methodology suited to Korean SMEs. Even though there are some evaluation methodologies, they are not applicable to Korean SMEs ; most of methodology are developed by U.S.A or Japan ; no systematic steps are provided; current information techonology are not incorporated; and just conceptual model and empirical studies are conducted. The objective of this study is to develop a methodology for evaluating information infrastructure in Korean SMEs. The methodology is providing systematic evaluation procedures incorporating the business environment of Korean SMEs and current information technology. In order to develop and verify the methodology, several research methodologies such as literature review and case study are conducted. The results show that the evaluation factors are valid and useful in Korean SMEs environment. Future research direction would be focusing on investigation of potential evaluation factors, conducting more surveys to various SMEs, and developing software package for automation of evaluation procedures.

미숙아에서 총 정맥영양으로 인한 담즙 정체성 황달 발생에 관한 임상적 고찰

황지성,김영민,문순정,배종우,최용묵 경희대학교 2002 慶熙醫學 Vol.18 No.3-4

중년 여성에서 발생한 집합관 신세포암 1 예

황인성;정재현;유지형;성락희;노충희;정재용;김현정 인제대학교 2011 仁濟醫學 Vol.32 No.-

Collecting duct renal cell carcinoma (CDRCC) or Bellini duct carcinoma is a rare type of renal cell carcinoma (RCC), accounting for 0.4% to 1.8% of all RCC. CDRCC is considered to originate from the collecting duct of the kidney and it has a poor prognosis. However, the clinical features of CDRCC remain poorly understood, mainly because only studies with a small series or case reports have been published to date. We experienced a case of CDRCC of kidney.

Effect of Chlorella Diet Supplementation on Blood and Urine Cadmium Levels in Cadmium Poisoned Rats

김용호,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3

This study was conducted to investigate the influence of chlorella diet supplementation. Blood accumulation and urine excretion levels were measured after the Sprague-Dawley (SD) rats were fed on a chlorella diet supplementation mixed with 40 ppm of CdCl₂. Four groups tested for blood accumulation and urine excretion levels. All four groups fed on a basic diet with a cadmium mixture. The diet for the first group contained only basic diet and the cadmium added to the drinking water. The diet for the three other groups contained cadmium to the drinking water, and 1%, 5% and 10% of chlorella added to the basic diet. A concentration of cadmium for the first group showed a 3.2±0.4 ㎍/l blood accumulatior. level and 41.5±32.9 ㎍/l urinary excretion level, and the second group, which was fed on the basic diet with 1% of chlorella added and cadmium to the drinking water showed a 1.5±0.6 ㎍/l blood level and only 14.1±1.6 ㎍/l urinary excretion level. The other two groups, which were fed on 5% and 10% of chlorella concentration and cadmium to the drinking water did not exhibit any notable effects greater than the group fed on 1% concentration of chlorella. The results suggest that the blood accumulation and urinary excretion of Cadmium are influenced by the chlorella diet supplementation from the concentration of 1% of the basic diet.

교정치료에 따른 사춘기 성장 아동의 상기도 폭경과 안면 성장 변화와의 관계 연구

김윤지,복규석,이규홍,황용인,박양호 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.3

본 연구의 목적은 아동에서 사춘기 성장 동안 기도의 변화가 안면 성장에 미치는 영향을 평가하는 것이었다. 9 - 11세(평균 10.7세)의 교정환자 36명을 대상으로 하여 초진 시(T1)와 치료 종료 시(T2)에 각각 측모두부방사선사진과 수완부 방사선 사진을 촬영하였다. 측모두부방사선사진상의 기도 폭경을 기준으로 하여 대상을 세 그룹으로 나누었다; 폭경이 좁은 그룹(AW-Narrow: 5.2 - 8.6 mm), 중간 그룹(AW-Medium: 8.9 - 11.5 mm), 넓은 그룹(AW-Wide: 11.7 - 16.0 mm). T1과 T2시기에 각 그룹 간의 안면 계측치의 차이를 비교하였으며 T1 - T2 사이의 계측치의 변화량을 각 그룹별로 비교하여 성장을 평가하였다. 각 그룹의 기도 폭경 증가량은 AW-Narrow, AW-Medium, AW-Wide group에서 각각 4.55 mm, 3.84 mm, 1.94 mm였으며 통계적으로 유의한 차이를 보였다. T1시기의 각 그룹 간의 비교에서 AW-Narrow group에서 다른 그룹에 비해 PFH가 유의하게 작은 값을 나타냈다. T1 - T2 사이의 성장 평가에서는 AW-Narrow group에서 다른 그룹에 비해 PFH/AFH, facial axis, ANS (T1 - T2), and Gn (T1 - T2)의 변화가 유의하게 큰 값을 보였다. 이 시기의 안면 성장 기간 동안 기도 폭경이 좁은 그룹에서 기도 폭경 및 안면 성장의 보상성 변화가 일어난 것으로 생각된다. 바람직하지 않은 안면 성장을 예방하기 위한 외과적 기도 처치를 고려할 때 자발적인 개선 가능성을 고려하여 좀 더 신중한 평가가 필요할 것으로 생각된다. Objective: The purpose of this study was to evaluate how airway changes influence facial growth during puberty in Korean children. Methods: Thirty-six patients aged 9 to 11 (mean age: 10.7 years) were selected. Cephalograms and hand-wrist x-rays were taken at pre-treatment (T1) and post-treatment (T2). The sample was divided into narrow (5.2 . 8.6 mm, AW-Narrow), medium (8.9 . 11.5 mm, AW-Medium), and wide (11.7 . 16.0 mm, AW-Wide) groups according to the airway width at T1. Cephalometric measurements at T1, T2 and growth from T1 to T2 were compared between groups. Results: The degree of increase in airway size in each group was 4.55 mm, 3.84 mm and 1.94 mm in the AW-Narrow, AW-Medium, and AW-Wide groups, respectively. Moreover, the differences were statistically significant. The significant smallest posterior facial height was found in the AW-Narrow group at T1. For the growth values from T1 to T2, the AW-Narrow group showed significantly larger values of PFH/AFH, facial axis, ANS (T1 - T2), and Gn (T1 - T2) than the AW-Medium group. Conclusions: The compensational changes in the airway width and facial growth were found in the narrow group. Surgical approach of the airway to prevent unfavorable facial growth in these years of age should be carried out, but with careful deliberation because these problems may be improved naturally.

신축 공동주택의 VOCs와 알데히드류 성분에 대한 실태조사

임득용,김지현,황인조,김동술 경희대학교 2004 環境硏究 論文集 Vol.13 No.-

In comparison with other studies, in particular, the emission of indoor HCHO in new apartments were very serious and Vacs showed below the Japan's guidelines (260 ㎍/㎥) excepting toluene (324.31 ㎍/㎥). Concentration of volatile organic compounds (VOCs) including aldehydes in indoor air was analyzed at 3 new apartments (at the lower, middle and upper floors of each apartment) during the Spring in 2004. The sample gases of Vacs and aldehydes were collected by Silicon canister (6 L) and 2,4-DNPH cartridges, and the analysis was made by the GC/MSD and PLC. As a result of the analysis, formaldehyde (HCHO) amongst aldehydes was found the highest (874.63 ppb) at the upper floor of C Apt., and the aldehydes' concentrations were S > C > K Apt. each. Also it did not show an apparent variation of characteristics according to floor. The concentration of VOCs was the highest (819.22 ppb) at the upper floor of C Apt. which exhibited in proportion with height.