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Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.
( Hui Qin Zhang ) 복식문화학회 2014 服飾文化硏究 Vol.22 No.6
The paper focuses on the clothing codes described in the Analects of Confucius, and explores the reasons for the rules setting up, including the rules of color, the design, the pattern, and even the material of the clothes whose distinct features reflect the ceremony and etiquette of the time in the classic, which are in correspondence with the requirements of Confucianism, especially for stressing the social rights, obligations and codes of conduct to everyone in the society.
Method of stress field and stability analysis of bedding rock slope considering excavation unloading
Hui Qin,Xiaotao Yin,Hua Tang,Xu Cheng,Hao Yuan 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.10
The stress state of a slope and its analytical solution are prerequisites for stability analysis. Currently, the limit equilibrium method cannot consider the stress state of slopes, and the solution of bedding slope stress remains in numerical simulations for a long time. In this paper, based on the theory of the elastic stress solution of the wedge top under a concentrated load, the analytical solution of the stress field was obtained using Matlab programming. Unloading stress analysis method (USAM) for determining the sliding surface of the bedding rock slope, safety factor (Fs), and reinforcement forces (Fr) were proposed. The USAM is compared with the Limit Equilibrium Method (LEM) and Finite Element Method (FEM) in terms of the Fs, Fr, sliding surface position, and sliding surface stress. The results show: 1) The analytical stress of the slope can be obtained quickly and conveniently using the USAM, while subsequent programming helps in faster completion of the stability evaluation of the bedding rock slope. Fs, Fr, sliding surface position, and sliding surface stress are all close to the results obtained by the FEM. 2) The USAM is more suitable for analyzing slopes when the angle between the slope surface and structural plane is greater than 20° (θ > 20o) and the slope angle is greater than 45o (β > 45o). 3) Through the analysis of specific engineering cases, the safety factor calculated by USAM are 0.06 and 0.02 larger than those obtained by LEM and FEM, respectively, and the required reinforcement force for the slope is closer to that obtained by FEM. The novel stress and stability analysis method allows for the rapid evaluation and optimal engineering design of bedding rock slope.
진혜금 ( Hui Qin Chen ) 고려대학교 중국학연구소 2007 中國學論叢 Vol.21 No.-
``讀圖時代``의 도래를 눈앞에 두고, 讀圖를 조망하여 보는 것은 저차원적인 독법일까? 아니면 의식적으로 讀圖時代를 인도하는 讀圖 문화일까? 본 글에서는 중국 고대 그림에서의 심미적 특색과 讀圖방식에 대한 분석을 통해, 현대 讀圖의 3가지 특성을 살펴보았다. 첫 번째는 회화의 공간의식이 특정한 시간의식이 되었다는 것이고, 둘째는 형상에 대한 미적 감상이 개인적인 문화적 취향이 된 것이며, 셋째는 피동적으로 받아들여진 것이 주동적인 창작으로 된 것이다. 사람들이 그림을 감지하는 과정에서 그것의 圖外音、景外意과 象外情을 읽어내야만 하며, 독일의 고전 회화와 고전음악의 발달이 문학가와 사상가의 문학적 상상력과 논리적 사유에 영향을 미치지 않았듯, 사람들의 읽기능력이 讀圖時代의 도래로 인해 쇠퇴할 리는 없을 것으로 믿는다.
Zhang, Hui,Wang, Shouzhi,Li, Hui,Yu, Xijiang,Li, Ning,Zhang, Qin,Liu, Xiaofeng,Wang, Qigui,Hu, Xiaoxiang,Wang, Yuxiang,Tang, Zhiquan Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10
Abdominal fat characters are complex and economically important in the poultry industry. Their selection may benefit from the implementation of marker-assisted selection (MAS). The objective of this study was to identify the markers linked to QTL responsible for fatness traits. The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the study. A total of 596 individuals from the divergent tails from the 6th to the 10th generations were genotyped at 23 microsatellite markers on chromosome 1. The differences of allele frequencies of all marker alleles between the divergent tails across the five generations were recorded. The allele frequencies of five markers, including LEI0209, LEI0146, MCW0036, ADL328 and MCW0115, had significant differences between the two tails in all five generations. The resulting p-values using Fisher's exact test on eleven markers, containing MCW248, MCW0010, MCW0106, LEI0252, LEI0068, MCW0018, MCW0061, LEI0088, MCW200, MCW283 and ROS0025, had a decreasing tendency from the 6th to the 10th generation. Statistical analysis showed that polymorphisms of the eight markers, including LEI0209, LEI0146, ROS0025, MCW0115, MCW0010, MCW0036, MCW283, ADL328, were significantly (p<0.0011) or suggestively (p<0.05) associated with abdominal fat content (AFW and AFP) across generations. It is concluded that the eight markers could be associated with the QTL affecting the deposition of abdominal fat in broiler chickens.
Regulatory Role of SFN Gene in Hepatocellular Carcinoma and Its Mechanism
Ying Hui,Hao Zeng,Yi Feng,Wenzhou Qin,Peisheng Chen,Lifang Huang,Wenfu Zhong,Liwen Lin,Hui Lv,Xue Qin 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3
Purpose: This study aims to explore the differential expression of SFN gene and its regulatory role in different hepatocarcinoma cells, and the impact on hepatocarcinoma. Materials and Methods: High and low SFN expression cells were screened by qRT-PCR and western blotting methods. SFN over expression and interference vectors were constructed. Cell viability was detected by CCK8 kit, cell cycle and apoptosis were detected by flow cytometry. Cell invasion and migration were detected. CCNB1 and CDK1 expression levels were detected by qRT-PCR and Western blotting methods. Results: The high SFN expression BEL7402 cells and the low SFN expression Hep3B cells were screened from Hep3B, HepG2, and BEL7402 cells. The activity of Hep3B cells overexpression vector SFNpcDNA3.1(+) decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of S phase increased. The activity of BEL7402 cells transfected with SFN siRNA decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of G2/M increased. Interference and overexpression vectors have little effect on the invasion and migration of the two cells. The expression of CDK1 in Hep3B cells decreased significantly, the expression of CDK1 and CCNB1 in BEL7402 cells increased significantly. Conclusions: The differentially expressed SFN gene can regulate the growth of the two hepatocarcinoma cells, high expression of SFN gene can inhibit their growth. The mechanism may be achieved by regulating CCNB1 and CDK1 expression.