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      • KCI등재
      • SCOPUSKCI등재

        Development of Benthic Community on an Artificial Reef Complex, Jeju Island, Korea

        Yi, Soon-Kil,Huh, Hyung-Tack,Je, Jong-Geel,Kim, Dae-Gwen Korea Institute of Ocean Science Technology 2001 Ocean and Polar Research Vol.23 No.3

        Development process of benthic community on experimental substrata attached on a newly installed artificial reef complex in Jeju Island was observed for 27 months from 1992 to 1994. Among 34 species of algae and 64 species of zoobenthos obtained from the study, Ecklonia cava dominated with a maximum biomass of about $10kg/m^2$. It was able to smother the other animals, however it provided a new substrate for the new settlers. Opportunistic bryzoans such as Likenopora radiata and Dexiospira spirillum occurred during the early stage were substituted by poriferans, banacles and other bryzoans. Young barnacles were smothered by bryozoans, on the contrary, bryozoans were bulldozed by adult barnacles. No apparent differences have been observed between vertically and horizontally installed substrates in terms of species composition and biomass during the early stage of succession. Thereafter owing to the rapid growth of E. cava, the horizontal substrata carried on a higher biomass while the vertical showed a higher coverage. The benthic process on the experimental substrata can be classified into three stages: initial stage, build up stage and regulatory stage. Important mechanisms involved were canopying of E. cava, suffocation by bryozoans and poriferans, and bulldozing of adult barnacles.

      • KCI등재

        어류 혈액 성분의 저장 안정성

        전중균,허형택,김병기 한국수산학회 1995 한국수산과학회지 Vol.28 No.2

        조피볼락, 잉어 및 무지개송어의 혈청을 상온(15℃), 저온(4℃), 냉동(-20℃)으로 보존하면서 총단백질(TP), 알부민(ALB), 중성지방(TG), 콜레스테를(CHOL), 포도당(GLC), 인(P), 나트륨(Na), AST 및 ALT의 활성 변화를 상온과 저온에서는 16일까지, 냉동에서는 30일까지 조사하였다. 어종간에 다소의 차이가 있기는 하지만 대체로 단백질 성분인 TP, ALB과 당 성분인 GLC, 무기질인 P, Na의 농도는 안정하였고, 지방 성분인 TG와 CHOL, AST, ALT의 활성은 불안정하였다. 그리고 온도에 따른 혈액 성분의 안정성은 상온에서 보다는 저온과 냉동에서 대체로 안정하지만, 성분에 따라서는 냉동하여도 매우 불안정한 것이 있다. An attempt was made to elucidate the stability of serum metabolites and enzyme activities in blood samples taken from rockfish(Sebastes schlegeli), Israeli carp(Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) under different storing conditions. The concentrations of total protein(TP), albumin(ALB), triglyceride(TG), cholesterol(CHOL), glucose (GLC), phosphorus(P) and sodium(Na), and the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were investigated for 16 days at 15℃(room temp.) and 4℃ (refrigerative) condition, or frozen at -20℃ for period of 30 days. Though there was a little difference between fish species, the concentrations of TP, ALB, GLC, P, Na in serum were stable at all storing temperatures, while those of TG, CHOL, ALT and AST were not stable, particularly even at the normal temperature. In general, serum components were more stable at refrigerative and frozen conditions than at room temp. storing. However, it was noticeable that the stability of CHOL in rockOsh serum was found to be more unstable at -20℃ than kept at 15℃ or 4℃.

      • 양식(養植) 진주담치의 마비성패독(痲痺性貝毒)

        전중균,허형탁,Jeon, Joong-Kyun,Huh, Hyung-Tack 한국해양학회 1989 韓國海洋學會誌 Vol.24 No.2

        Attempts were made to analyze the toxin composition of the toxic mussel Mytilus edulis galloprovincialis which were collected from aquaculture pond in Apr. 1988 in Hachung, Koje, southern Korea. The toxins were partially purified from the ethanolic extract of the mussel digestive glands by activated charcoal and Bio Gel P-2 column chromatography. HPLC analysis demonstrated that the toxin consisted mainly of gonyautoxin 1-4 (GTX 1-4), along with trace amounts of saxitoxin (STX) and protogonyautoxin 1-2 (PX 1-2).

      • KCI등재

        주요 양식 어류의 혈액 성분에 관한 연구

        박용주,전중균,허형택,김병기 한국수산학회 1995 한국수산과학회지 Vol.28 No.2

        우리 나라에서 많이 양식하고 있는 주요 어종의 혈액 성분에 관한 기초 자료를 얻기 위하여, 저수온기(수온 12.7℃)에 월동중인 조피볼락(Sebastes schlegeli), 농어(Lateolarax japonicus), 넙치(Paralichthys olivaceus), 돌돔(Oplegnathus fasciatus)과 함께 냉수성 어종인 은연어(Oncorhynchus kisutch)를 각 5∼6마리씩 마취시키지 않은 상태로 꼬리 동맥에서 채혈하여 혈청 중의 총단백질(TP), 알부민(ALB), 중성 지방(TG), 콜레스테롤(CHOL), 포도당(GLC), 리파제(LIPA), 나트륨(Na), 칼륨(K), 염소(Cl), 인(P), 아미노산전이효소(ALT, AST) 등을 혈액분석기로 분석하였다. TP 농도는 2.9∼5.1g/㎗의 수준이었으며, ALB 농도는 1.2∼1.9g/㎗의 수준이었다. A/G 비는 은연어가 1.1 정도로 가장 높았고 나머지는 0.5∼0.6 정도로 비슷하였다. 지질 성분인 TG와 CHOL 농도도 어종에 따라 달라, 넙치는 TG 농도가 큰 대신에 CHOL 농도가 낮았으며, 농어는 반대로 TG농도가 낮은 대신에 CHOL농도가 높았다 두 지질 성분의 합은 넙치가 가장 많아 600㎎/㎗나 되었고, 농어나 조피볼락(약 400㎎/㎗), 돌돔이나 은연어(약 300㎎/㎗)의 순이었다. 혈당(GLC) 농도는 냉수성 어종인 은연어가 넙치에 비해 약 4배 가량 많아 61-76㎎/㎗이나 되었다. 한편 리파제(LIPA) 활성은 TG농도와 정반대의 경향을 보여 은연어에서 가장 높았고, 넙치에서는 거의 확인되지 않았다. 무기질 중에서 Na, Cl과 K 농도는 160∼204m㏖/ℓ, 137∼183m㏖/ℓ과 0.5∼3.1m㏖/ℓ로 유사한 수준이었으나, P 농도는 은연어나 조피볼락처럼 비교적 활동성이 강한 어종일수록 많았다. ALT 활성은 넙치가 가장 높았고, AST 활성은 은연어가 높았으며, AST 활성은 모든 어종에서 ALT 활성보다 높았다. This study was performed to obtain the basic data on the serum components of several marine fish species commonly cultured in Korea. Blood samples taken from five species of fish were analyzed for various components of serum, total protein (TP), albumin (ALB), triglyceride (TG), cholesterol (CHOL), glucose (GLC), sodium (Na), potassium (K), chloride (Cl), phosphorus (P), lipase (LIPA), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The fish used were coho salmon (Oncorhynchus kisutch), rock fish (Sebastes schlegeli), sea bass (lateolabrax japonicus), olive flounder (Paralichthys olivaceus) and parrot fish (Oplegnathus fasciatus) reared at the Chungmu Experimental Fish Culture Satation of KORDI. TP concentration of warm-water species (2.9∼5.1 g/㎗) was higher than that of cold-water species, and ALB concentration was ranged at the level of 1.2∼1.9 g/㎗. Coho salmon showed the highest ration of A/G(1.1), and the other species were about 0.5∼0.6. The concentrations of TG and CHOL, components of lipids, varied with the different species. The concentration of TG was high, but CHOL concentration was low in olive flounder, while the reversed results were shown by sea bass. The sum of these two components was the highest with 600㎎/㎗ in olive flounder, and about 400㎎/㎗ for sea bass and rock fish, and 300㎎/㎗ for parrot fish and coho salmon. Concentration of GLC in coho salmon and rock fish ranged from 61 to 76㎎/㎗ which were about four times higher than that of flounder. The highest lipase activity was observed in coho salmon, while it was nearly nil in flounder. The reversed tendency was found for TG concentration. Mineral concentrations of Na, Cl and K were 160∼204 m㏖/ℓ, 137∼183m㏖/ℓ and 0.5∼3.lm㏖/ℓ, respectively, but no significant difference between the species was observed. However, the concentrations of P were high in relatively active species such as coho salmon and rockfish. AST activity in all species examined was higher than that of ALT with being highest in coho salmon. The highest ALT activity was found in olive flounder.

      • 무지개송어(Oncorhynchus mykiss) 사료원으로써 대두박의 이용 효과 II. 소화율, 배설 및 체조성 변화

        김병기,전중균,허형택,조재윤,Kim Pyong Kih,Jeon Joong-Kyung,Huh Hyung Tack,Jo Jae-Yoon 한국양식학회 1996 韓國養殖學會誌 Vol.9 No.4

        사료 단백질원으로써 대두박의 생물학적 이용성을 평가하기 위하여 무지개송어를 160일 동안 사육하면서 사육 후 60일 및 120일째에는 대두박 사료의 소화율, 암모니아의 배설 경향 및 어체의 성분 분석을 실시하였다. 실험 사료로는 단백질원으로 어분을 $64.5\%$ 사용한 대조구 및 상품 탈지 대두박을 $10\~70\%$까지 등간격으로 늘린 사료를 사용하였으며, 비교를 위하여 상품용 무지개송어 사료를 사용하였다. 실험 사료의 단백질 및 지질 소화율은 대두박 함량이 많을수록 단백질 소화율은 증가하였지만, 지질 소화율은 오히려 감소하는 경향을 나타내었다. 또한, 무지개송어의 전어체 및 조직(간 및 근육)의 일반 성분에서 대두박 함량이 많은 공급구일수록 지질 함량이 적었으나 전어체의 아미노산 및 지방산 조성에는 큰 차이가 없었다. 그리고 단백질의 최종 대사산물인 암모니아($NH_3-N$)의 배설량을 섭이 후 24시간 동안 조사하였더니, 배설은 주로 후반 12시간에 많아 총 배설량의 2/3 이상이나 되었고, 24시간의 총 배설량은 대두박 함량이 많을수록 증가하여 대두박함량 $70\%$ 공급구가 $466.8\;mg{\cdot}kg\;body\;wt.^{-1}day^{\-1}$으로 대조구의 $115\%$ 수준이었다. 이상과 같이 대두박 함량이 많은 사료는 단백질의 소화 흡수에 문제가 있는 것이 아니고 그보다는 에너지원 중에서 지질의 이용성이 크게 떨어지는 듯 하였다. A feeding experiment for 160 days was conducted to evaluate effects of dietary soybean meal (SBM) on the apparent digestibility, ammonia excretion and body composition of rainbow trout (Oncorhynchus mykiss). Seven diets containing $0\~70\%$ SBM were formulated based on iso-nitrogenous and iso-caloric basis of $44\%$ crude protein and approximately 4,200 Kcal/kg of gross energy. To evaluate the nutritional utilization of the SBM diets at the end of 60 and 120-day rearing with test diets, digestion rates of protein and lipid of SBM diets were measured. Fish fed the diets containing above $46\%$ SBM showed higher apparent digestibility for protein, but lower for lipid than did fish fed the control diet in both trials. Protein and fat contents in the carcass were similar for all experimental fish, except for fish fed $58\%$ and $70\%$ SBM which showed lower fat content than the others, but composition of fatty acid and amino acids were not affected by dietary SBM levels. Gill and urinary post-prandial ammonia ($NH_3-N$) excretions were measured at 12 and 24 hours after single feeding of the experimental diets. Excretions of $NH_3-N$ measured after 24 hours were almost 1 times higher than those measured after 12 hours. Total excretion fer the 24 hours by fish fed $70\%$ SBM was $15\%$ higher than that of fish fed the control diet. Results of present study may suggest that the rainbow trout which were fed above $34%\;or\;46\%$ of SBM diet showed a decrease gradually in lipid bioavailability compared to the control group.

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