The mechanism of enhanced photocatalytic activity of SnO2 through fullerene modification

Shuang-Shuang Ding,Wei-Qing Huang,Bing-Xin Zhou,Ping Peng,Wangyu Hu,Meng-Qiu Long,Gui-Fang Huang 한국물리학회 2017 Current Applied Physics Vol.17 No.11

Carbon nanomaterials are prominent building blocks in the synthetic van der Waals (vdW) heterostructures with desired properties. Scientific understanding of their interfacial interactions is the premise to design this kind of vdW heterostructures with optimal performance.We here study the mechanism of enhanced photocatalytic activity of SnO2 by fullerene modification at electronic level, to explore the interfacial interaction and its correlation with photocatalytic activity. The results show that the interfacial interaction increases with the number of C atom of fullerene, and leads to some of C atoms be positively/ negatively charged, making the fullerene a highly active co-catalyst in heterostructures. Compared to pristine SnO2, the band gap of the heterostructures is much smaller, leading to their absorption wavelength extending the entire visible region. Interestingly, a staggered type-II band alignment in the C20 (C60)/SnO2 (101) heterostructures results into the robust separation of photoexcited charge carriers between the two constituents, indicating that the fullerene is an effective sensitizer, and thus enhanced photocatalytic activity. These findings can rationalize the available experiment and will be of broad interest in developing the highly efficient semiconductor photocatalysts via fullerene modification.

A Light-weighted Data Collection Method for DNS Simulation on the Cyber Range

( Shuang Li ),( Shasha Du ),( Wenfeng Huang ),( Siyu Liang ),( Jinxi Deng ),( Le Wang ),( Huiwu Huang ),( Xinhai Liao ),( Shen Su ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.8

The method of DNS data collection is one of the most important parts of DNS simulation. DNS data contains a lot of information. When it comes to analyzing the DNS security issues by simulation on the cyber range with customized features, we only need some of them, such as IP address, domain name information, etc. Therefore, the data we need are supposed to be light-weighted and easy to manipulate. Many researchers have designed different schemes to obtain their datasets, such as LDplayer and Thales system. However, existing solutions consume excessive computational resources, which are not necessary for DNS security simulation. In this paper, we propose a light-weighted active data collection method to prepare the datasets for DNS simulation on cyber range. We evaluate the performance of the method and prove that it can collect DNS data in a short time and store the collected data at a lower storage cost. In addition, we give two examples to illustrate how our method can be used in a variety of applications.

Enzymatic reduction of polyunsaturated lysophosphati-dylcholine hydroperoxides by glutathione peroxidase-1

Huang, Long Shuang,Kim, Mee Ree,Sok, Dai-Eun WILEY-VCH Verlag 2009 European journal of lipid science and technology Vol.111 No.6

<P>Some lipid peroxides are known to be converted to their corresponding alcohols in cells containing glutathione peroxidase (GPx). In this respect, we examined the enzymatic conversion of lysophosphatidylcholine (lysoPC) hydroperoxides to hydroxyl derivatives using RBL-2H3 cells and erythrocyte GPx-1. First, the incubation of RBL-2H3 cells with arachidonoyl lysoPC led to the formation of a major product, with maximal UV absorbance at 234 nm and m/z [M+H]<SUP>+</SUP> at 560.2, corresponding to monohydroxyeicosatetraenoyl lysoPC. Similarly, linoleoyl lysoPC was also converted to its hydroxyl derivative in RBL-2H3 cells. Separately, lysoPC hydroperoxide, generated from soybean lipoxygenase 1-catalyzed oxygenation of linoleoyl lysoPC, arachidonoyl lysoPC or docosahexaenoyl lysoPC, was converted by GPx-1 to the corresponding hydroxyl derivatives. When the kinetic values were determined, the K<SUB>m</SUB> values (3.1–32.3 µM) of the polyunsaturated lysoPC hydroperoxides increased with decreasing number of double bonds, in contrast to a similar value of V<SUB>m</SUB> among them. Moreover, the catalytic efficiency of docosahexaenoyl lysoPC hydroperoxide was much greater than that of H<SUB>2</SUB>O<SUB>2</SUB> as substrate of GPx-1. In related experiments, where phosphatidylcholine hydroperoxides were incubated with phospholipase A<SUB>2</SUB> and GPx-1, the complete conversion of phosphatidylcholine hydroperoxides to hydroxyl derivatives was confirmed by LC/MS. Taken together, it is proposed that GPx-1-type enzymes may participate in the conversion of polyunsaturated lysoPC hydroperoxides to hydroxyl derivatives in cell systems.</P>

Lysophosphatidylcholine Containing Docosahexaenoic Acid at the sn-1 Position is Anti-inflammatory

Huang, Long Shuang,Hung, Nguyen Dang,Sok, Dai-Eun,Kim, Mee Ree Wiley (John WileySons) 2010 Lipids Vol.45 No.3

Phytate Determination in Various Cultivars of Korean Rice

Huang, Long Shuang,Sok, Dai-Eun,Kim, Hyoung-Chin,Yoon, Won-Kee,Kim, Hwan-Mook,Kim, Mee-Ree The Korean Society of Food Science and Nutrition 2006 Preventive Nutrition and Food Science Vol.11 No.1

To determine the amount of phytate in rice grains from various cultivars, two methods were employed and compared in respect of the accuracy and conveniency. Phytate in rice samples was extracted with HCl, and then the extracts were subjected to an anion-exchange column. Finally, the phytate in eluate was quantitated using two methods: one method is based on the complex formation between ferric ion and sulfosalicylic acid in the presence of phytate, and the other is the prior acid digestion of phytate sample, followed by the colorimetric determination of liberated phosphorus. Although two methods showed similar values of phytate in rice samples, the former method is simpler and more precise than the latter. Moreover, the former is more reliable for the samples with lower phytate levels. Especially, the dilution condition of rice sample before anion exchange column separation was important for the recovery of phytate in rice samples. Based on the former method, the amount of phytate in rice of various cultivars was estimated to range from 7.3 mg/g to 12.4 mg/g rice. This method would be useful for the determination of phytate in crop samples with a lower level of phytate, one of anti-nutrients in some agricultural plants.

Prediction of protein-protein interactions based on protein-protein correlation using least squares regression.

Huang, De-Shuang,Zhang, Lei,Han, Kyungsook,Deng, Suping,Yang, Kai,Zhang, Hongbo BENTHAM SCIENCE PUBLISHERS 2014 CURRENT PROTEIN AND PEPTIDE SCIENCE Vol.15 No.6

<P>In order to transform protein sequences into the feature vectors, several works have been done, such as computing auto covariance (AC), conjoint triad (CT), local descriptor (LD), moran autocorrelation (MA), normalized moreaubroto autocorrelation (NMB) and so on. In this paper, we shall adopt these transformation methods to encode the proteins, respectively, where AC, CT, LD, MA and NMB are all represented by '+' in a unified manner. A new method, i.e. the combination of least squares regression with '+' (abbreviated as LSR(+)), will be introduced for encoding a protein-protein correlation-based feature representation and an interacting protein pair. Thus there are totally five different combinations for LSR(+), i.e. LSRAC, LSRCT, LSRLD, LSRMA and LSRNMB. As a result, we combined a support vector machine (SVM) approach with LSR(+) to predict protein-protein interactions (PPI) and PPI networks. The proposed method has been applied on four datasets, i.e. Saaccharomyces cerevisiae, Escherichia coli, Homo sapiens and Caenorhabditis elegans. The experimental results demonstrate that all LSR(+) methods outperform many existing representative algorithms. Therefore, LSR(+) is a powerful tool to characterize the protein-protein correlations and to infer PPI, whilst keeping high performance on prediction of PPI networks.</P>

Preparation and Performance Evaluation of a Zinc Oxide-Graphene Oxideloaded Chitosan-Based Thermosensitive Gel

Huang Hao,Han Rui,Huang Ping-Ping,Qiao Chuan-Yue,Bian Shuang,Xiao Han,Ma Lei 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.6

This study aimed to develop and assess a chitosan biomedical antibacterial gel ZincOxideGrapheneOxide/Chitosan/β-Glycerophosphate (ZnO-GO/CS/β-GP) loaded with nano-zinc oxide (ZnO) and graphene oxide (GO), known for its potent antibacterial properties, biocompatibility, and sustained drug release. ZnO nanoparticles (ZnO-NPs) were modified and integrated with GO sheets to create 1% and 3% ZnO-GO/CS/β-GP thermo-sensitive hydrogels based on ZnO-GO to Chitosan (CS) mass ratio. Gelation time, pH, structural changes, and microscopic morphology were evaluated. The hydrogel's antibacterial efficacy against Porphyromonas gingivalis, biofilm biomass, and metabolic activity was examined alongside its impact (MC3T3-e1). The findings of this study revealed that both hydrogel formulations exhibited temperature sensitivity, maintaining a neutral pH. The ZnO-GO/CS/β-GP formulation effectively inhibited P. gingivalis bacterial activity and biofilm formation, with a 3% ZnO-GO/CS/β-GP antibacterial rate approaching 100%. MC3T3-e1 cells displayed good biocompatibility when cultured in the hydrogel extract.The ZnO-GO/CS/β-GP thermo-sensitive hydrogel demonstrates favorable physical and chemical properties, effectively preventing P. gingivalis biofilm formation. It exhibits promising biocompatibility, suggesting its potential as an adjuvant therapy for managing and preventing peri-implantitis, subject to further clinical investigations.

Determination of Phytate in Korean Rice of Various Cultivars

Long-Shuang Huang,Dai-Eun Sok,Hyoung-Chin Kim,Won-Kee Yoon,Hwan-Mook Kim,Mee Ree Kim 동아시아식생활학회 2006 동아시아식생활학회 학술발표대회논문집 Vol.- No.-

Phytate Determination in Various Cultivars of Korean Rice

Long Shuang Huang,Dai-Eun Sok,Hyoung Chin Kim,Won Kee Yoon,Hwan Mook Kim,Mee Ree Kim 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.1

To determine the amount of phytate in rice grains from various cultivars, two methods were employed and compared in respect of the accuracy and conveniency. Phytate in rice samples was extracted with HCl, and then the extracts were subjected to an anion-exchange column. Finally, the phytate in eluate was quantitated using two methods: one method is based on the complex formation between ferric ion and sulfosalicylic acid in the presence of phytate, and the other is the prior acid digestion of phytate sample, followed by the colorimetric determination of liberated phosphorus. Although two methods showed similar values of phytate in rice samples, the former method is simpler and more precise than the latter. Moreover, the former is more reliable for the samples with lower phytate levels. Especially, the dilution condition of rice sample before anion exchange column separation was important for the recovery of phytate in rice samples. Based on the former method, the amount of phytate in rice of various cultivars was estimated to range from 7.3 ㎎/g to 12.4 ㎎/g rice. This method would be useful for the determination of phytate in crop samples with a lower level of phytate, one of anti-nutrients in some agricultural plants.

Biological Actions of Polyunsaturated Fatty Acyl Lysophospholipids

Long Shuang Huang,Sok, Dai-Eun 충남대학교 약학대학 의약품개발연구소 2009 藥學論文集 Vol.24 No.-

Lysophosphatidylcholine (lysoPC) and polyunsaturated fatty acids are known to be generated from the hydrolysis of phosphatidylcholine by enzymes such as secretary phospholipase A₂, lipoprotein-specific phospholipase A₂, lecithin/cholesterol acyl transferase or endothelial lipase. Free polyunsatuarted fatty acids have been known to be oxygenated by lipoxygenases to produce various bioactive compounds, which are known to exert antioxidant and anti-inflammatory actions. Meanwhile, lysoPC and its hydrolysis product, lysophosphatidic acid (lysoPA), are known to express a variety of bioactivities. However, most of the studies on the bioactivities of lysoPC and lysoPA were examined using lysophospholipids with saturated fatty acyl chains. Recently, the activities of unsaturated lysophospholipids have been of increasing interest, since unsaturated lysophospholipids, present to a substantial level in vivo, have been reported to be utilized as substrates for enzymes such as lipoxygenase. In this review, the biological activities of lysophospholipids, saturated or unsaturated, were discussed in an attempt to stress on the peculiar activities of polyunsaturated lysophospholipids such as 1-linoleoyl lysoPC and 1-docosahexaenoyl lysoPC.