SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells

Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Dong, Fa-Wu,Luo, Huai-Rong,Wan, Qin-Li,Xu, Feng-Qing,Fan, Wei-Wei,Wang, Kai-Jin,Li, Ning,Hu, Jiang-Miao Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-${\beta}$-D-glucopyranoside (1) and 3,4,4',5-tetramethoxybibenzyl-3'-O-${\beta}$-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).

Analysis on Deformation Behavior and Restructuring of Additive Manufactured Metal Lattice-Structures under Compressive Loading Condition

진칭예(Jin Qing Ye),강동석(Dong Seok Kang),연시모(Si Mo Yeon),손용(Yong Son),박상후(Sang Hu Park) Korean Society for Precision Engineering 2020 한국정밀공학회지 Vol.37 No.8

We studied compressive behavior of two types of lattice structures having small-scale struts fabricated by utilizing a metal additive manufacturing process. Generally known, the lattice structure has some advantages such as lightweight and high specific mechanical strength, allowing diverse potential applications in the aerospace and mobility industries. In this work, we proposed two types of lattice such as body-centered truss (BCT) and octahedral truss (OCT) that were designed and fabricated for a compression test. From the experimental results, the OCT has much higher strength than the BCT, and all cases showed several buckling modes during the compressive behavior. Furthermore, ‘restructuring’ occurred with BCT, and the compressive force increased overall but fluctuated due to the restructuring by an increase of compression. Through this work, we found out that the BCT has the interesting compressive behaviors, and a repetitive bucking-restructuring was found. In fact, its strength could be increased continuously by the restructuring during compression. In conclusion, the BCT has key-characteristics of lightweight and re-strengthening, which are applicable to various applications in the industry.

Overexpression of Cyclooxygenase-1 Correlates with Poor Prognosis in Renal Cell Carcinoma

Yu, Zu-Hu,Zhang, Qiang,Wang, Ya-Dong,Chen, Jing,Jiang, Zhi-Mao,Shi, Min,Guo, Xin,Qin, Jie,Cui, Guang-Hui,Cai, Zhi-Ming,Gui, Yao-Ting,Lai, Yong-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

The aim of this study was to evaluate expression of COX-1 in renal cell carcinoma (RCC) and its prognostic value. mRNA of COX-1 was detected in 42 paired RCC and adjacent normal tissues with quantitative realtime polymerase chain reaction (qRT-PCR). Expression of COX-1 was also evaluated in 196 RCC sections and 91 adjacent normal tissues with immunohistochemistry. Statistical analysis was performed to assess COX-1 expression in RCC and its prognostic significance. The results of qRT-PCR showed mRNA levels of COX-1 in RCC tissues to be significantly higher than that in adjacent normal tissues (p < 0.001). Immunohistochemical assays also revealed COX-1 to be overexpressed in RCC tissues (p < 0.001). Statistical analysis demonstrated high expression of COX-1 was correlated with tumour size (p = 0.002), pathological stage (p = 0.003), TNM stage (p = 0.003, 0.007, 0.027, respectively), and tumour recurrence (p < 0.001). Survival analysis indicated patients with high expression of COX-1 had shorter survival time (p < 0.001), and COX-1 was an independent predictor. This is the first study to reveal overexpression of COX-1 in RRC and point to use as a prognostic marker in affected patients.

QTL Analysis of Floral Traits of Rice (Oryza Sativa L.) under Well-Watered and Drought Stress Conditions

Song Ping Hu,Ying Zhou,Lin Zhang,Xu Dong Zhu,Zheng Gong Wang,Lin Li,Li Jun Luo,Qing Ming Zhou 한국유전학회 2009 Genes & Genomics Vol.31 No.2

Three floral traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES) and dual exserted stigma (PDES) of a RI population with 185 lines under water stress and non-stress conditions for two years were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. High phenotypic correlation was found between PSES and PDES (r=0.5424***). Based on a linkage map of 203 SSR markers, when under well-watered condition, six QTLs (qSNP-3b, qSNP-4, qSNP-11 qSNP-2, qSNP-5 and qSNP-9) were detected for SNP. Half of them had significant Q×E interactions. Three QTLs (qPSES-1, qPSES-2, qPSES-5) were found to influence PSES, including one locus (qPSES-2) having Q×E interaction. And three QTLs (qPDES-2, qPDES-5 and qPDES-8) were also detected to influence PDES. qPDES-5 was found to have Q×E interaction. The contribution rate of a single QTL varied from 0.80% to 8.83% for additive effect, and 1.86% to 15.25% for Q×E interactions. Under drought stress, six QTLs (qSNP-3a, qSNP-4, qSNP-7a, qSNP-7b, qSNP-8 and qSNP-9) were associated with SNP, including qSNP-3a and qSNP-4 with Q×E interaction. Three QTLs (qPSES-1, qPSES-10 and qPSES-12) were located on rice chromosome 1, 10 and 12 for PSES. Four QTLs (qPDES-1a, qPDES-1b, qPDES-4 and qPDES-9) were detected for PDES, including qPDES-9 with Q×E interaction. The additive effect of single QTL can only explain 1.16% to 5.84% of total variance while Q×E interaction of four loci can explain 4.25% to 11.54% of total variance for each locus. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%~9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES and PDES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress conditions, indicating that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Fa-Wu Dong,Huai-Rong Luo,Qin-Li Wan,Feng-Qing Xu,Weiwei Fan,Kai-Jin Wang,Ning Li,Jiang-Miao Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-β-D-glucopyranoside (1) and 3,4,4',5- tetramethoxybibenzyl-3'-O-β-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).

Case-Control Study of Diet in Patients with Cervical Cancer or Precancerosis in Wufeng, a High Incidence Region in China

Jia, Yao,Hu, Ting,Hang, Chuan-Ying,Yang, Ru,Li, Xiong,Chen, Zhi-Lan,Mei, Ye-Dong,Zhang, Qing-Hua,Huang, Ke-Cheng,Xiang, Qun-Ying,Pan, Xiu-Yu,Yan, Yu-Ting,Wang, Xiao-Li,Wang, Shao-Shuai,Hang, Zhou,Tang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Purpose: To investigate the diet of patients with cervical cancer and precancerosis in the Wufeng area, a high-incidence region in China. Methods: In the case group, 104 patients diagnosed with cervical cancer or cervical intraepithelial neoplasias (CINII/III) were recruited from the Wufeng area. Nine hundred thirty-six healthy women were selected from the same area as the matched controls. A questionnaire, which included questions about general lifestyle conditions, smoking and alcohol status, source of drinking water, green tea intake, and diet in the past year, was presented to all participants. Results: Green tea intake (P=0.022, OR=0.551, 95% CI=0.330-0.919) and vegetable intake (P=0.035, OR=0.896, 95% CI=0.809-0.993) were identified as protective factors against cervical cancer or CINII/III. There was no indication of any associations of other lifestyle factors (smoking status, alcohol status, source of drinking water) or diet (intake of fruit, meat/egg/milk, soybean food, onion/garlic, staple food and pickled food) with cervical cancer. Conclusions: The results suggest that eating more fresh vegetables and drinking more green tea may help to reduce the risk of cervical cancer or CINII/III in people of the Wufeng area.

Low Expression of Tyrosine-protein Phosphatase Nonreceptor Type 12 is Associated with Lymph Node Metastasis and Poor Prognosis in Operable Triple-negative Breast Cancer

Wu, Min-Qing,Hu, Pan,Gao, Jie,Wei, Wei-Dong,Xiao, Xiang-Sheng,Tang, Hai-Lin,Li, Xing,Ge, Qi-Dong,Jia, Wei-Hua,Liu, Ren-Bin,Xie, Xiao-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Low tyrosine-protein phosphatase nonreceptor type 12 (PTPN12) expression may be associated with breast cancer growth, proliferation, and metastasis. However, the prognostic value of PTPN12 in breast cancer has not been clearly identified. Patients and Methods: 51 triple-negative breast cancer (TNBC) patients and 83 non-TNBC patients with a histopathology diagnosis from October 2001 to September 2006 were included in this study. Immunohistochemical staining for PTPN12 on tissue microarrays was conducted. Results: High PTPN12 expression was seen in 39.2% of TNBC and 60.2 % of non-TNBC cases. Low PTPN12 expression was associated with lymph node status (p = 0.002) and distant metastatic relapse (p = 0.002) in TNBC patients. Similarly, low PTPN12 expression in non-TNBC patients was significantly correlated with lymph node status (p = 0.002), stage (p = 0.002) and distant metastatic relapse (p = 0.039). The high PTPN12 expression group was associated with longer DFS and OS compared with low PTPN12 expression group only in TNBC cases (p = 0.005, p = 0.015), according to univariate Cox regression analysis. Conclusion: These findings provide evidence that low expression of PTPN12 is associated with worse prognosis and may be used as a potential prognostic biomarker in TNBC patients.

Curcumin Induces Apoptosis and Inhibits Growth of Human Burkitt’s Lymphoma in Xenograft Mouse Model

Zai-xin Li,Ke-qing Ouyang,Xv Jiang,Dong Wang,Yinghe Hu 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.3

Curcumin, a natural compound extracted from rhizomes of curcuma Curcuma species, has been shown to possess potent anti-inflammatory, anti-tumor and anti-oxidative properties. However, the mechanism of action of the compound remains poorly understood. In this report, we have analyzed the effects of curcumin on the cell proliferation of Burkitt’s lymphoma Raji cells. The results demonstrated that curcumin could effectively inhibit the growth of Raji cells in a dose- and time-dependent manner. Further studies indicated that curcumin treatment resulted in apoptosis of cells. Biochemical analysis showed that the expression of Bax, Bid and cytochrome C were up-regulated, while the expression of oncogene c-Myc was down regulated after curcumin treatment. Furthermore, poly (ADP-ribose) polymerase (PARP) cleavage was induced by the compound. Interestingly, the anti-apoptotic Bcl-2 expression was not significantly changed in Raji cells after curcumin treatment. These results suggested that the mechanism of action of curcumin was to induce mitochondrial damage and therefore led to Raji cell apoptosis. We further investigated the in vivo effects of curcumin on the growth of xenograft tumors in nude mice. The results showed that curcumin could effectively inhibit tumor growth in the xenograft mouse model. The overall results showed that curcumin could suppress the growth of Burkitt’s lymphoma cells in both in vitro and in vivo systems.

Role of a Novel Pyridostigmine Bromide-Phospholipid Nanocomplex in Improving Oral Bioavailability

Qun-you Tan,Jing-qing Zhang,Ni-ni Hu,Guo-dong Liu,Hua-feng Yin,Li Zhang,Hong Wang,Lu-yang Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X1, X2, and X3 were 8, 40oC, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and −25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (Tmax) 2 h vs 2 h, the maximum concentration (Cmax) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC0-∞) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.