Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

성견 절치에서 수직 골결손 형성후 함입이동시 치주조직의 반응

박양수,김영준,최홍란,황현식 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The present study was performed to investigate the periodontal response following orthodontic intrusion of teeth with vertical bony defect produced by periodontal disease. Vertical bony defect and periodontal disease were produced adjacent to the both maxillary second lateral incisors in four adult dogs, weighing 20kg or more. Four weeks later, a flap operation was performed to eliminate inflammation and a reference notch was made on the root surface at the level of bottom of each defect. Two weeks after periodontal surgery, four weeks of intrusion and another four weeks of retention was executed on the right incisors while left incisors served as the control. Through the histologic analysis, following results were obtained. 1. The periodontal lesion which was produced by elastics and artificial bony defect was characterized by three walled infrabony pocket. 2. In the histologic section of control side, the base of the pocket was located below the level of the cemento-enamel junction. Connective tissue attachment was formed apically from the base of the pocket. 3. On the experimental side, the base of the pocket was localized close to the cemento-enamel junction. Connective tissue attachment was formed near to pocket base and extended apically. 4. New cementum was formed from the notch coronally toward the epithelial junction along the root surface in the both intruded incisors and control. 5. In the fluorescence microscopic observation, bone regeneration was began at the base of defect and the remodelling pattern characterized by a combination of coronal bone generation and resorption of the marginal crest could be detected. Although the remodelling pattern was the same in the both side, the bone remodelling in intruded incisors was continued during tooth movement. The results of the present study suggest that an orthodontic intrusion of extruded incisors with vertical bony defect can be carried out with a desirable periodontal response, provided that both the oral hygiene and the biomechanical force system are kept under control.

전치부 치간이개의 수복종류에 따른 유한요소법적 응력분석

박상현,양홍서 전남대학교 치과대학 1989 전남치대논문집 Vol.1 No.2

The purpose of this study was to analyze the stress of restoration, tooth and adjacent supporting structures according to the width of anterior diastema and the type of restorations. The finite element models of right and left central incisors were divided into four groups according to the type of restorations of each model were inputed to the two dimensional finite element program and stresses were analyzed. Results were as follows: 1. In case of non-splint group, high tensile stress in distocervical area and slight compressive stress in contact point were observed, and compressive and tensile stress were distributed widely in dentin and supporting tissues. 2. In case of splint group, high compressive and tensile stresses were concentrated to splinting area and the stress concentrations of dentin and supporting tissues were less distribution than those of non-splint group, since almost all the stresses were received in metal framework. 3. In the single unit PFM group, as the width of the diastema was increased, the tensil stress was increased in the distocerivical area of porcelain. 4. In the PFM group, the concentration of tensile stresses were seen in the porcelain layer below the metal framework in splint group, whereas stresses were concentrated to distocervical area in non-splint group. 5. The highest compressive and tensile stresses were concentrated to splint area in resin splint group of all groups. 6. The stresses concentration of compact bone were seen mainly in the distal surface in splint group, whereas stresses were distributed widely in non-splint group.

일간지 생산-배송계획 문제를 위한 혼성유전알고리듬 해법

박양병,홍성철 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

많은 기업들은 그들의 생산과 배송 기능을 분리하여 각각의 최적화를 꾀하고 있는데, 이러한 접근방법에 의해 이룰 수 있는 절약은 제한적일 수밖에 없다. 최근의 공급사슬경영(SCM)에서는 이들 두 기능의 관련 비용을 총체적 관점에서 잘 조절하면서 동시에 통합 분석하는 것이 더 중요하다고 인식되고 있다. 본 논문에서는 일간지의 생산-배송계획 문제를 다룬다. 일간지는 재고로 유지될 수 없으며, 고객이 정한 시간보다 늦게 배송되면 큰 손실을 야기하는 일종의 단일기간 재고품목이다. 저자들은 일간지 생산-배송의 통합계획을 위한 혼성유전알고리듬 해법을 개발하고 예제를 이용하여 Hurter and Buer의 순차적 분리계획 해법과 성능을 비교한다. 계산실험 결과, 개발된 해법의 성능은 효과적인 것으로 나타난다.

계분으로부터 살모넬라에 감염된 가금류의 신속한 진단

박홍양 동물자원연구센터 1999 1999년도 동물자원연구센터 학술심포지움 Vol.1999 No.-

우리의 건강과 깊은 관계가 있는 유해미생물중 하나인 살모넬라균은 최근 심각한 사회문제로 대두되고 있다. 가금류인 닭의 경우 살모넬라가 주로 장내에 번식하고 있어 도살시 계육뿐만 아니라 계분과 함께 배출되어 산란된 난에까지 오염이 이어지고 있다. 더불어 육계, 산란계로부터 조기에 살모넬라 감염여부를 파악하는 것이 중요한데 현재의 검출법 중 하나인 단순배양법의 경우 시간이 오래걸리고 많은 인력과 비용이 드는 단점이 있다. 그래서 본 연구는 살모넬라에 감염된 닭의 분을 이용해 유전공학기법의 하나인 PCR(Polymerase Chain Reaction)을 이용해서 살모넬라균 특유의 유전자의 염기 서열을 찾아 검출에 필요한 primer(S-1,S-2,S-7,S-8)를 제작하고, 이를 적절하나 조건(94 ℃에서 30초간의 denaturation, 63 ℃에서 20초간 annealing, 72 ℃에서 40초간의 extension 과정을 30번 반복)에 맞추어 실험하여 닭의 도축전에 감염여부를 신속하게 판단하여 양계농가의 경제적 이익과 국민의 건강증진에 초점을 두고 있다.

말초성 신경아세포종 : 두개골 발생 증례 보고 Involving Skull

박양화,권익승,류연석,신미경,최문배,홍승관,문명선 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.4

The peripheral neuroblastomas are malignant neoplasms that originate from the adrenal gland and sympathetic nervous tissues. They usually occur in childhood. They exhibit such malignant features that they metastasize early to lung, liver, bone, rarely skull and other structures. The patients with peripheral neuroblastoma have short duration of symptom and short survival period. They reveal the characteristic light microscopic features that resemble other small cell carcinomas. The establishment of final diagnosis of peripheral neuroblastoma needs various special stainings for small cell carcinomas, and the electron microscopic findings are the most reliable. The authers recently experienced a case of peripheral neuroblastoma in a 26-year old man which involved right frontoparietal skull vault. The only chief complaint was a local non-tender mass at right frontoparietal scalp. Neither headache nor any neurological deficits was detected. Simple skull X-ary revealed a punched out rediolucency at right frontoparietal bone and brain CT showed a superficial elliptical high density mass that enhanced strongly. The mass was totally removed by wide craniectomy. The tumor invaded and penetrated the dura but the arachnoid membrane. at the tumor bed was not invaded by the tumor. The tumor was confirmed as peripheral neuroblastoma by various special stainings for small cell carcinomas. Following surgical resection of the mass, post-operative radiotherpy was offered(4800 rads for about 5 weeks). The patient aggrevated progressively and showed numerous metastases to such bones as lumbar vertebrae, pelvis and humerus to became paraplegic. 8 months after the operation, the patient died.

마우스의 장내 농양(intraabdominal abscess)형성에 대한 연구

박장환,정용훈,조양자,김정목,전윤홍 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

Intraabdominal abscesses (IAA) are produced microbiologically by mixed infection. And clinical treatment of IAA is limited because duration of the these diseases are prolong and the their nature is mixed infection. This study was carried out to develop a standardized IAA model used in B.fragilis. Encapsulated B.fragilis and autoclaved colonic and cecal contents produced IAA> And encapsulated B.fragilis produced IAA more than unencapsulated B. fragilis. Only E.coli was not effective to produce IAA but combination with encapsulated B.fragilis produced IAA. Abscesses were usually found in the serosal surface adherent to pancrease or colon and in the fatty tissue around the uterus. This study will subsequently support a development of IAA treatment.

전신마취중 에피네프린사용으로 발생되는 부정맥

박선규,진홍용,양홍석 중앙대부설한국의과학연구소 1985 한국의과학 Vol.17 No.3

Opaque의 두께가 전장용 레진의 색채에 미치는 영향

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

The purpose of this study was to evaluate the color difference of specimens with the variation of metal type and opaque thickness. Resin veneered specimens with the same color were fabricated and evaluated by the CIE L*a*b* system. Specimens were divided into 4 groups as follows ; Group P1; Resin with precious metal alloy, 0.1 mm opaque layer Group P2; Resin with precious metal alloy, 0.5 mm opaque layer Group B1; Resin with nonprecious metal alloy, 0.1 mm opaque layer Group B2; Resin with nonprecious metal alloy, 0.5 mm opaque layer The results obtained were as follows ; 1. L* value of group P1 was lower than that of group P2. There was no significant differences in a* values among tested group. 2. Opaque thickness did not produce any significant differences of L*, a*, b* value in the resin veneer groups on the base metal. 3. The ΔE*ab value between P1 and P2 was 2.228 which is noticeable, while the color difference between B1 and B2 was slight.

4주간의 유연성 프로그램이 기록향상과 요통자각도에 미치는 효과

박원익,양점홍,윤양진 釜山大學校 附設 體育科學硏究所 2001 體育科學硏究所 論文集 Vol.17 No.-

The purpose of this study was to determine the effect of 3 type(arch of foot beating technique, forward walk grabbed with ankle, PNF[proprioceptive neuromuscular facilitation]) flexibility exercise program during 4 weeks on the improvement of record and visual analogue scale of low back pain. The subjects of this study were 15 (the third grade of male high school students) who are applying to department of physical education and had no low back pain problem and being trained the comprehensive physical fitness exercise more than 3 months. The test item was sit and reach and the results were as the follows; 1. The group of PKF showed higher than the groups of arch of foot beating technique and forward walk grabbed with ankle in the change of sit and reach test score. The change of sit and reach test score was a significant difference in correlation among the group·among the exercise period ·among the group and exercise period. 2. The group of arch of foot beating technique showed higher than the group of PNF and forward walk grabbed with ankle in the change visual analogue scale of low back pain. The change of visual analogue scale of low back pain was a significant difference in correlation among the group·among the exercise period·among the group and exercise period.