Surface Plasmon Resonance 기술을 이용한 인체분비물 혈액형 분석에 대한 연구

홍승범,최동호,안희중,이동석,김응수,최상규,이영보 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

삼환계 항우울제(Dothiepin)가 우울증 환자의 수면에 미치는 영향 : 수면 다원 검사에 의한 연구 A Polysomnographic Study

홍승철,한진희,이성필,방승규 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.4

우울증환자군에서 삼환계 항우울제인 dothiepin 투여 후의 수면이 변화양성을 알아보며, 치료 후의 호전정도를 예측할 수 있는 치료 전과 치료 후의 수면변인을 조사하기 위하여 본 연구를 시행하였다. 수면상태는 수면다원검사를 실시하여 평가하였고, 우울증상의 심각도와 임상양상의 정도는 Hamilton 우울척도를 이용하여 평가하였다. 연구대상은 최소 14일 이상 약물을 투여 받지 않은 우울증 환자 16명(남자 7명, 여자 9명, 연령분포 23∼64세)으로 구성되었다. 수면다원검사는 약물을 투여하기 전과 약물투여 후 1주와 3주에 시행하여 다음과 같은 결과를 얻었다. 1) 항우울제 투여 1주와 3주 후 수면 연속성은 기준수면에 비하여 총수면 시간이 유의하게 증가하였고 수면잠재기는 단축되었으며, 수면도중 각성시간은 감소하였고, 수면효율은 증가되었다. 2) 항우울제 투여 1주와 3주 후 수면구조에서는 기준수면에 비하여 2단계 수면이 유의하게 증가하였다. 3) 항우울제 투여 1주와 3주 후 급속안구운동 수면은 기준수면에 비하여 유의하게 감소하였고, 급속안구운동 수면잠재기는 증가하였다. 4) 항우울제 투여 1주 후와 3주 후의 수면을 비교시 수면잠재기가 3주에 유의하게 감소된 외에는 수면 변인의 차이를 보이지 않았다. 5) 우울증상의 평가를 위하여 약물투여 1주 후와 3주 후에 시행한 Hamilton 우울척도 점수는 약물투여 전에 비하여 줄어들어 우울증상이 호전됨을 나타내었다. 6) 항우울제 투여 3주 후 치료반응이 좋았던 군의 수면특징은 기준수면에서 수면도중 각성시간이 증가된 경향을 나타내었고, 약물투여 1주 후의 수면에서는 4단계 수면의 양이 유의하게 감소되었다. 이상의 결과를 볼 때, 우울증환자에서 삼환계 항우울제인 dothiepin 투여 후의 수면변화는 치료초기에 나타났다. 기준수면에서 각성기간의 증가와 1주 후의 수면에서 4단계 수면의 감소는 약물치료에 좋은 반응과 관련된 수면변인으로 생각되었다. Objectives : This study was designed to investigate 1) sleep changes after antidepressant (dothiepin) treatment, and 2) sleep variables which seem to be associated with clinical response in the depressed patients. Methods : The subjects consisted of 16 patients who fullfilled the criteria for major depression by the Diagnostic and statistical Manual, (4th edition). Their sleep was recorded using polysomnography at the baseline and after one week and three weeks of dothiepin treatment. All subjects were further interviewed using Hamilton Rating Scale for Depression (HRSD) to rate the severity of their depression. High response to the drug was defined as a reduction of more than 50% of the HRSD score. Results : The results were as follows : 1) Depressed patients after dothiepin treatment showed more total sleep time(p=0.019), shorter sleep latency(p=0.05), less awake time(p=0.033), more sleep efficiency(p=0.018), more stage 2 sleep(p=0.002), less REM time(p=0.000), and longer REM sleep latency(p=0.004) than before treatment. 2) There were no differences in sleep variables between those who received 1 week and 3 weeks of dothiepin treatment except for the shortening of sleep latency after 3 weeks(p<0.05). 3) Depressive symptom scores on HRSD were reduced after 1 week and 3 weeks of dothiepin treatment as compared with the baseline. 4) High responders showed a tendency of increased wake time(p=0.054), while their stage 4 sleep decreased after 1 week of dothiepin treatment as compared with the low responders(p=0.0136). Conclusion : These results suggest that sleep of the depressed patients after dothiepin treatment tends to be normalized and sleep changes seem to appear early in the treatment phase. In addition, clinical response might be associated with greater wake time at the baseline and lesser stage 4 sleep after 1 week of dothiepin treatment.

경식도 심초음파도의 Direct Planimetry를 이용하여 측정한 대동맥판 면적의 심박출량 및 좌심실 구출율의 변화에 따른 정확도

진승원,김종진,김희열,박지원,전두수,윤호중,박준철,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

Hanger로 보강된 스터드 접합부의 인장거동에 관한 실험적 연구

박휘규,김승훈,이리형,이용택,황홍순,이정희 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

This paper presents the tensile behavior of the stud connection between reinforced concrete(RC) and steel members. Hanger reinforcements are placed around the studs to transfer the tensile loads to the opposite side of the concrete member. Six specimens, of which variables were arrangement details of hanger reinforcements and studs. Tensile test results show that hanger reinforcements are effective to increase tensile strength and ductility. Test tensile strengths of specimens are compared with the nominal strengths by ACI codes(ACI 318-02, ACI 349-99) and PCI 1999.

動力耕耘機用 디젤 機關의 騷音特性

崔圭洪,李承奇,安義成 건국대학교 산업기술연구원 1994 건국기술연구논문지 Vol.19 No.-

국내에서 생산보급되고 있는 動力耕耘機用 8PS 디젤기관의 騷音 狀態를 파악하고자 消音器 전방 1m 위치 및 운전자 위치에서의 모터 구동시와 연소 구동시 각 회전수별 騷音을 측정 비교 분석하고 기관에서 발생하는 소음중 에너지 비율이 가장 높은 排氣音을 제어하기 위해 소음기를 부착시와 소음기 제거시 및 空洞의 體積을 변화시킨 경우의 각 回轉數別 騷音度, 燃料消費率, 出力 등을 측정 분석하여 현 소형 디젤기관의 騷音 減少方案 모색의 기초자료로 제시하고자 실시한 시험의 결과는 다음과 같다. 1) 機關의 回轉數가 증가함에 따라 騷音은 높아지는 것으로 나타났으며, 기존 機關의 경우 機械騷音은 85dB 이상으로 이의 騷音低減對策이 필요하다고 생각된다. 2) 消音器 附着時와 除去時 무부하 운전시와 전부하 운전시 騷音은 소음기 부착시가 제거시에 비해 각각 4, 13.1dB 정도 낮게 나타나 비교적 소음감쇄 효과가 높았다. 3) 空洞體積 變化에 따른 消音器別 騷音은 기존의 소음기 T-Ⅰ 보다 空洞의 체적을 증가시킨 T-Ⅱ, T-Ⅲ가 운전자 위치 및 소음기 전방 1m 위치에서 無負荷 運轉時 각각 1.9∼2.3dB 및 2∼2.8dB, 全負荷 運轉時 각각 4.9∼5dB, 7.1∼7.5dB 감소한 것으로 나타났다. 4) 각 회전수별 燃料消費率은 T-Ⅰ, T-Ⅱ, T-Ⅲ가 거의 같은 경향을 나타냈으나, 소음기를 제거한 경우는 약 17g/PS·hr 정도 낮게 나타났다. 또, 출력은 소음기를 부착한 경우가 제거한 경우에 비해 각각 0.55PS 낮게 나타났다. 5) 排氣騷音을 최대한으로 감소시키기 위하여 금후 機關出力 감소가 적고, 騷音 減少效果가 높은 形態의 消音器 開發硏究가 이루어져야 할 것으로 생각된다. This study was conducted to evaluate the noise characteristic of 8PS agricultural diesel engine through the modification, was found to provide the foundamental data that could be used to reduce the noise of the engine. To evaluate the noise characteristics on the agricultural diesel engine by the muffler, noise level, specific fuel consumption and output were measured in case of the with and without muffler and three different volume of muffler cavity. The results of the study are summarized as follows: The noise increased by the increament of revolution velocity. The mechanical noise of power tiller indicated more than 85dB at 1,800∼2,200rpm. Therefore, to minimized bearing ability of operator, it was thought that the countermeasure of noise reduction need. The noise level with the muffler was smaller than those without muffler by 4dB, 13.1dB. respectively. In case of increased the volume of muffler cavity, the noise level at the position of working seat was decreased 1.9∼2.3dB at the time of no-load condition and 4.9∼5dB at the time of full load, respectively. The specific fuel consumption of the without muffler type was 17g/PS hr lower than the with muffler type. The output of the without muffler type was 0.55PS higher than the with muffler type, respectively. To reduce of the exhaust noise, it is necessary to continue researches on the relationship between complex factors of the noise.

자외선/과산화수소 고도산화공정에 의한 하수 2차 처리수 중의 용존유기물 분해에 미치는 부유물질과 과산화수소 농도의 영향

안규홍,이석헌,안석,김기팔,정민우,맹승규 대한상하수도학회 2002 상하수도학회지 Vol.16 No.3

Secondary effluent of activated sludge process was treated by UV/H_2O_2 advanced oxidation process to investigate the effect of suspended matter and H_2O_2 concentration. With low dosage of UV radiation, the concentration of the dissolved organic compounds measured as DOC could be increased with and without hydrogen peroxide. It was suggested that the cause of DOC increase in treated samples was the decomposition of suspended microbial particles and the incomplete oxidation of dissolved organic compounds. The removal of suspended solid significantly enhanced the degradation of DOC in both UV and catalyzed UV oxidation. Color and odor of secondary effluent was effectively removed. Turbidity was decreased by oxidation and the particle size distribution was shifted to lower range. With increased H_2O_2 dosage (30∼120mg/l), the removal of DOC was enhanced. To obtain the required quality meeting the regulated level for water reuse, additional treatment for turbidity rejection was required.

축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

麥類條播機用 上向切削 로터리의 碎土性과 所要動力에 關한 硏究(Ⅰ)

崔圭洪,洪顯裕,李承奇 建國大學校 附設 農業資源開發硏究所 1993 農資源開發論集 Vol.18 No.-

본 시험은 麥類畦立細條播機 逆回轉 로터리 作業時 耕深을 16cm로 고정하고 耕耘 피치 및 토양수분 차이가 PTO軸의 토크와 所要動力 排水溝의 형상 및 쇄토율 등에 미치는 영향을 구명하기 위하여 벼 수확을 마치고 벼그루가 남아 있는 埴壤土의 논에서 시험을 실시하였으며 주요 결과는 다음과 같다. 1) PTO軸의 平均토크 및 最大토크는 토양수분에 따라 차이는 있지만 逆回轉 로터리의 耕耘 토크는 耕耘 피치 6cm이하에서는 耕耘 피치 증가에 따라 직선적으로 증가하지만 耕耘 피치 6cm이상에서는 耕耘 피치 증가에 관계없이 거의 일정한 경향이었다. 2) 作業速度와 排水溝 殘存흙 및 覆土狀態는 逆回轉 로터리의 주행속도가 느릴수록 또 PTO回轉速度가 빠를수록 양호하여 주행속도 1단 PTO 3단에서 가장 좋게 나타났다. 3) 쇄토율은 耕耘 피치가 증가할수록 또 토양수분이 높을수록 쇄토율은 떨어지는 경향이었으며, 충분한 碎土程度를 얻기 위하여는 耕耘 피치를 3cm이하로 하고 가능한 토양수분 함량 31.7%(d.b) 이하에서 작업하는 것이 좋을 것으로 판단되었다. 4) PTO軸의 所要動力은 작업속도 1∼4단 로터리 變速段數 1∼3段에서의 PTO軸 所要動力은 토양수분 38.0%(d.b)일 때 5.1∼14.5ps, 35.5%(d.b)의 경우 7.9∼14.2ps, 31.7%(d.b)일 때는 4.6∼12.7ps로 나타나 토양수분이 증가할수록 소용동력은 증가하다가 다시 감소하는 경향이었다. 5) 覆土狀態는 耕耘 피치가 적을수록 양호하여 주행속도 1단 PTO 3단의 경우 가장 좋았으며, 작업능률면을 고려할 때 排水溝 程度, 覆土狀態, 所要動力 및 作業能率 등을 고려할 때 畦立 로터리 파종작업시 PTO變速段數는 3단으로 하고 주행단수는 토양수분 함량 31.7%(d.b) 내외에서는 3단, 토양수분함량 35.5%(d.b) 내외에서는 2단, 토양수분 함량 38.0%(d.b) 이상에서는 1단으로 작업하여야 할 것으로 판단된다. This study was carried out to investigate the soil pulverization rate and power requirement of up-cut rotary of wheat and barley drill seeder for tractor by tilling pitch and soil moisture content. The experiment was conducted in the paddy field of clay roam remaining stem of paddy rice after harvesting. The results are summarized as follows: 1) Average and maximum torque of PTO shaft was increased in proportion with tilling pitch in less than 6cm, but was apt to definite over than 6cm of tilling pitch. 2) The working speed, status of remaining and covering soil in drainage was good in proportion with decrease the travelling speed of up-cut rotary and increae the revolutions per minute. Therefore, the working status in drainage was best in forward first gear and PTO third stage. 3) The soil pulverization rate was decreased according to increase the tilling pitch and soil moisture content. It was concluded that tilling work in 31.7 percent of soil moisture content and less than 3cm of tilling pitch would be good. 4) The power requirement of PTO shaft by soil moisture content were 5.2∼14,5ps in 38.0 percent, 7.9∼14.2ps in 35.1 percent and 4.6∼12.7ps in 31.7 percent soil moisture content. So the power requirement of PTO shaft was apt to increase in proportion with soil moisture content the first and decrease the last. 5) The status of soil covering was good in forward first gear and PTO third stage. When it was considered the status of drainage, covering the soil and power requirement in working performance, it was concluded that the PTO stage would be third and working speed would be forward third gear in 31.7 percent, forward second gear in 35.5 percent and forward first gear in 38.0 percent of moisture content.

주암호 상류지류의 수질에 관한 연구 : 사평천을 중심으로 On The Sapyung River

주흥규,홍승호,백종오 조선대학교 약학연구소 1998 藥學硏究誌 Vol.19 No.1

The water quality of Sapyung stream, one of four branches of Lake Chuam, was investigated in August and October. 1995. The results are as follows : 1. The pH of all the investigated sites was ranged from 5.5 to 7.6 which is similar to ordinary streams in Korea, which is an evidence that there it was no source to change the pH significantly 2 In the first investigation DO was ranged from 5.5 to 7.4 ppm, which was slightly lower ppm than other streams, because it was higher atmospheric temperature(35℃) and water temperature(30.5℃) It was ranged from 7.3 to 7.4 ppm in the second investigation, indicating that site is relatively stable and clear area. 3. BOD during all the investigation was ranged from 1.0 to 2.7ppm, indication that it is good water quality. 4. NH₃-N, indicator of water pollution was ranged from 0.03 to 0.07ppm, It is believed that the source was not the contamination of the water 5. NO₂-N and NO₃-N were slightly higher in the first investigation than the second investigation. This is a result of better nitrification in the first investigation because it was longer daylight time and higher water temperature. 6. Chlorine ion concentraton, total hardness, and PO₄-P were detected homogeneously in all investigated area. It is believed that they are due to characteristics of the ground and are not the evidence of point source contamination when compared to other streams. 7. The level of contamination due to general bacteria and E. coli was much lower than other streams, however, in the detection of 7.9x10² E. coli at sites 5, water quality of main stream of Lake chuam needs to be monitored closely.