A NAT for reliable HBV DNA Screening of Plasma

Hong SeungHwa,Kang HyeNa,Noh HyangSoon,Lee NamKyoung,Kim SoonNam,Hong ChoongMan,Lee KiHong,Oh HoJung,Yoo SiHyung,Lee SeokHo,Choi SeungEun,Shin InSoo 大韓藥學會 2002 大韓藥學會 總會 및 學術大會 Vol.2002 No.2

Health Promotion and Health Service/Program Use on an Urban Campus in the U.S.

Hong Seokho 한국보건사회연구원 2015 保健社會硏究 Vol.35 No.3

이 연구의 목적은 도시대학 캠퍼스를 중심으로 학생들의 건강을 증진시키기 위해 교내에서 제공 되어지고 있는 건강관련 보건서비스(혹은 프로그램)의 이용실태와 영향 요인들을 분석하기 위함이다. 임의 선택된 2400명의 학생 중에 536명(응답률 22%)이 2011년 가을학기의 온라인 서베이에 응답하였고 결측값을 제외한 429 케이스를 본 연구 분석을 위하여 사용하였다. 교내 서비스에 대한 이용패턴을 분석하기 위해 엔더슨의 건강행동모델(Andersen의 의료이용모형)을 적용하였다. 교내 건강서비스 이용 인자를 찾기 위해 이용그룹과 이용하지 않은 그룹간의 비교를 위해 위계적 로지스틱 회귀 분석 기법이 이용되었다. 구체적인 서비스 별 인자는 서로 다른 패턴으로 발견이 되었다. 학생 보건센터(student health service) 이용은 높은 인지도, 전일제, 높은 학년의 학생 일수록 이용 확률이 높은 것으로 나타났다. 흥미롭게도 남녀 사교클럽(Greek organization)의 학생일수록 보건센터 방문 확률이 높았으나, 자원봉사단체의 학생일수록 방문 확률이 낮았다. 학생 상담실(counseling Center)이용은 많은 나이, 소수 인종, 학교와의 높은 접근성, 높은 학년, 그리고 낮은 정신건강 지표의 학생 일수록 이용 확률이 높은 것으로 나타났다. 건강관련 강좌(wellness hub)의 이용은 높은 신체건강 지표와 소수인종의 학생일수록 이용 확률이 높은 것으로 나타났다. 수면실과 안마의자 서비스 (relaxation room)는 낮은 접근성과 음악과 미술관련 동아리 회원일수록 낮게 나타났다. 다양한 교내 서비스를 필요로 하는 학생들에게 적절하게 서비스가 제공되기 위해 서비스 별 체계적인 접근법을 가지고 학생들에게 홍보되어져야 한다. The purpose of the present study was to find the main predictors for the utilization of campus health-related services in order to figure out the help-seeking process of urban graduate students seeking a healthy life. Of 2,400 students, 536 were randomly selected (response rate: 22%) and recruited. Selected students responded to an online survey for September and October in 2011. In order to figure out the main predictors and their effects, five sets of binary hierarchical logistic regression analyses were conducted among students who used the campus services and other students who did not use them. Generally, system adjustments and social activities were closely associated with the utilization of campus health-related services. In specific service use, all had different predictor patterns. The student health service was more associated with awareness of service and system adjustments such as attendance status and semester. The counseling center was more related to age, race, physical accessibility and system adjustments, and emotional need. Minority group and physical health status were important predictors of the wellness hub use. A relaxation room was related to physical accessibility and social activities. This study suggests that the Andersen Behavioral Model can be applicable in informal campus health-related service use. Various services are needed with different approaches to encourage students in need to use services.

Acculturation of Older Asian Immigrants Focusing on Chinese, Filipino, Japanese, Korean, and Vietnamese

Seokho Hong 청주대학교 사회과학연구소 2023 한국사회과학연구 Vol.44 No.2

미국에서 거주하고 있는 아시아계 노인 이민자 수가 증가함에 따라 이들의 문화 적응에 대한 관심이 높아지고 있다. 본 연구는 캘리포니아에 거주하는 아시아계 이민 노인들 중 5개 소수 민족 하위 그룹(중국인, 일본인, 한국인, 필리핀인, 베트남인) 간의 인구학적 특성과 문화 적응의 차이를 비교하여 사회복지 실천적, 정책적 방안을 도출하는 것을 목표로 한다. 2차 데이터 분석이 사용되었으며 California Health Interview Survey(CHIS)에서 3,453명의 아시안 노인 이민자 샘플을 대상으로 최종 분석하였다. ANOVA 및 카이 제곱 테스트를 사용하여 그룹 차이를 조사했습니다. 연구 결과는 다음과 같다. 우선, 아시아 이민 노인들은 인종별 샘플은 동질적이지 않았다. 구체적으로 살펴보면, (1) 일본과 필리핀의 고령 이민자들은 중국, 한국, 베트남의 고령자들보다 더 나은 사회경제적 지위와 더 많은 자원을 가지고 있으며, (2) 고령의 일본 이민자들은 가장 문화에 잘 적응한 반면, 한국과 베트남의 고령 이민자들은 문화 적응 수준이 가장 낮게 나타났다. 또한 아시아 이민 노인들은 서로 다른 문화 적응 및 이민 경험을 가지고 있는 것으로 나타났다. 본 연구는 아시아 노인 이민자를 위한 문화적으로 경쟁력 있고 유능한 서비스 및 프로그램과 관련 정책으로 적극적인 개입의 중요성을 강조하는 제언을 제시하였다. As the elderly immigrants population increases in the US, attention to their acculturation has been increasing. This study aims to identify differences of demographic characteristics and acculturation among five Asian ethnic subgroups from China, Japan, Korea, Philippines, and Vietnam in California. The secondary data analysis was used, and a sample of 3,453 older Asian immigrants was analyzed from the California Health Interview Survey (CHIS). ANOVA and chi square tests to examine group differences were used. The older Asian immigrant samples were not homogeneous; (1) Japanese and Filipino immigrants had better socioeconomic status and resources than others and (2) older Japanese immigrants were most acculturated whereas older Korean and Vietnamese immigrants were least acculturated. Older Asians had different acculturation and immigration experiences. This study underscores to provide cultural adaptation services and programs for older Asian immigrants, and to build policy initiatives.

Crystal Structure of LysB4, an Endolysin from Bacillus cereus-Targeting Bacteriophage B4

Hong, Seokho,Son, Bokyung,Ryu, Sangryeol,Ha, Nam-Chul Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.1

Endolysins are bacteriophage-derived enzymes that hydrolyze the peptidoglycan of host bacteria. Endolysins are considered to be promising tools for the control of pathogenic bacteria. LysB4 is an endolysin produced by Bacillus cereus-infecting bacteriophage B4, and consists of an N-terminal enzymatic active domain (EAD) and a C-terminal cell wall binding domain (CBD). LysB4 was discovered for the first time as an L-alanoyl-D-glutamate endopeptidase with the ability to breakdown the peptidoglycan among B. cereus-infecting phages. To understand the activity of LysB4 at the molecular level, this study determined the X-ray crystal structure of the LysB4 EAD, using the full-length LysB4 endolysin. The LysB4 EAD has an active site that is typical of LAS-type enzymes, where $Zn^{2+}$ is tetrahedrally coordinated by three amino acid residues and one water molecule. Mutational studies identified essential residues that are involved in lytic activity. Based on the structural and biochemical information about LysB4, we suggest a ligand-docking model and a putative endopeptidase mechanism for the LysB4 EAD. These suggestions add insight into the molecular mechanism of the endolysin LysB4 in B. cereus-infecting phages.

Comparison on predictors of mental health service use among Asian older adults

Seokho Hong 한국사회복지학회 2019 Asian Social Work and Policy Review Vol.13 No.1

Studies have mostly examined mental health service use of older Asian immigrant combining all Asian Americans into one group whereas immigration backgrounds and socioeconomic status of each Asian minority group are different. Therefore, this study aimed to identify predictors of mental health service use within specific ethnic groups among older Asian adults focusing on Chinese, Japanese, Korean, Filipino, and Vietnamese in California. The Behavioral Model for Vulnerable Populations (BMVP) is used to guide the secondary data analysis of a sample of 3,453 older Asian immigrants from the California Health Interview Survey (CHIS). Logistic and linear regression analyses are performed to examine predictors of mental health service use and the frequency of mental health service use, respectively. As results, mental health‐seeking patterns differ by ethnicity within the older Asian immigrant sample, not being married (Korean), higher levels of acculturation (Filipino), lower levels of neighborhood cohesion (Korean and Vietnamese), higher levels of perceived safety (Korean) and lower levels of perceived safety (Vietnamese), higher levels of mental distress (Korean and Filipino), and having perceived need (all) were related to more visits for mental health services. The study findings highlight the necessity of cultural competency services and programs for each Asian ethnicity.

Comfort with discussions about death, religiosity, and attitudes about end‐of‐life care

Seokho Hong,John G. Cagle 한국사회복지학회 2019 Asian Social Work and Policy Review Vol.13 No.2

In end-of-life (EOL) care research, death anxiety and religiosity are often overlooked. Terror management theory (TMT) may provide a useful conceptual model with which to examine how comfort discussing death and religiosity influence attitudes related to EOL care. A telephone-based survey was conducted among community-dwelling adults in the Unites States. Via random sampling, with over-sampling of Hispanics/African Americans, 123 completed survey interviews (response rate = 46%) were ana-lyzed. Respondents were more likely to have better attitudes toward EOL care if they were older or white, religiously active, and comfortable with the subject of death. Religiosity and comfort discussing death were correlated with each other and re-mained significant predictors of attitudes about EOL care even without demographic covariates. Findings suggest that promoting an open dialogue about mortality may improve attitudes about EOL care and utilization of palliative care services. The study also provides evidence about the utility and applicability of TMT for EOL care.

Structure-based protein engineering of bacterial β-xylosidase to increase the production yield of xylobiose from xylose

Hong, Seokho,Kyung, Myungok,Jo, Inseong,Kim, Yong-Ro,Ha, Nam-Chul Elsevier 2018 Biochemical and biophysical research communication Vol.501 No.3

<P><B>Abstract</B></P> <P>Xylobiose consists of two molecules of xylose and has been highly recognized as a food supplement because it possesses high prebiotic functions. β-xylosidase exhibits enzymatic activity to hydrolyze xylobiose, and the enzyme can also catalyze the reverse reaction in the presence of high concentrations of xylose. Previously, β-xylosidase from <I>Bacillus pumilus</I> IPO (BpXynB), belonging to GH family 43, was employed to produce xylobiose from xylose. To improve the enzymatic efficiency, this study determined the high-resolution structure of BpXynB in a complex with xylobiose and engineered BpXynB based on the structures. The structure of BpXynB deciphered the residues involved in the recognition of the xylobiose. A site-directed mutation at the residue for xylobiose recognition increased the yield of xylobiose by 20% compared to a similar activity of the wild type enzyme. The complex structure of the mutant enzyme and xylobiose provided the structural basis for a higher yield of the engineered protein. This engineered enzyme would enable a higher economic production of xylobiose, and a similar engineering strategy could be applied within the same family of enzymes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The crystal structures of β-xylosidase from <I>Bacillus pumilus</I> in complex with xylobiose were determined. </LI> <LI> β-xylosidase was engineered to increase the yield of xylobiose based on the determined structures. </LI> <LI> The protein engineering strategy used in this study could be applied within the same family of enzymes. </LI> </UL> </P>

Impacts of Language Use, Family, School, and Neighborhood on Mental Distress: Analyzing Data on Immigrant Youth in California

Hong Seokho,Lee Sang Jung,Harrington Donna,Kim Moo-hyun 한국보건사회연구원 2017 保健社會硏究 Vol.37 No.2

본 연구는 미국내의 이민 청소년들의 정신 건강적 특징과 이민 경험을 파악하고, 이들의 심리적 피로감에 영향을 미치는 요인을 생태체계적 관점에서 분석하는 것이다. 캘리포니아 헬스 인터뷰 서베이(CHIS)로부터 미국에서 태어나지 않은 이민청소년 348명에 대한 데이터 정보를 분석에 사용하였다. 이민 청소년들의 심리적 피로감에 영향을 미칠 수 있는 개인 특성(나이, 성별, 인터뷰 언어, 미국 거주 기간), 가정(어른의 보살핌, 가정에서 사용하는 언어), 학교(학교에서의 지지, 또래 괴롭힘 유무), 그리고 지역사회 수준의 변수(이웃간 응집도)들을 위계적 다중 회귀모형을 통하여 분석하였다. 이민청소년들의 평균연령은 14.5세(표준편차= 1.7)이고 여성이 53.4%를 차지하였다. 응답자들 가운데 31.6%가 인터뷰 언어로 영어가 아닌 다른 언어를 사용하였고, 72.7%가 집에서 영어를 사용하지 않는 것으로 나타났다. 또한 대상자의 거의 절반에 가까운 48.6%가 미국에서 10년이상 거주하는 것으로 보고하였고, Kessler 등(2004)이 제시한 기준을 적용하면, 약 2%의 이민 청소년들이 심리적 피로감 검사에서 점수 13점 이상의 심각한 심리적 피로감을 가지고 있는 것으로 나타났다. 인터뷰 언어로 영어가 아닌 다른 언어를 사용했을 경우, 집에서는 영어를 사용하는 경우, 학교에서 지지와 지원이 높은 수준일 경우, 또래 괴롭힘을 당한 경험이 없는 경우, 그리고 이웃간의 응집도가 높은 수준일 경우 이민청소년들은 심리적 피로감이 낮게 나타났다. 본 연구의 결과들은 이민청소년들의 정신건강에 영향을 미치는 사용 언어, 그리고 학교와 이웃관련 요인들을 다룬 선행 연구와 이론에 함의를 제공한다. 또한 학교와 지역사회의 실천현장에서 이민청소년의 새로운 환경에 대한 적응 과정에서 겪게 되는 심리적 피로감을 감소시키기 위한 효과적인 프로그램과 서비스 개발의 필요성 또한 강조한다. This study aims to identify experiences of immigration and mental health related characteristics of non U.S.-born adolescents and to examine predictors related to mental distress within a social ecological perspective. Secondary data of 348 non U.S.-born adolescents from the CHIS 2011-2012 Adolescents Survey were used for this study. Impacts of individual, family, school, and community factors on mental distress of non U.S.-born adolescents were examined using hierarchical multiple regression. The average age of the adolescents was 14.5 years (SD = 1.7), and more than half were girls (53.4%). Of the participants, 31.6% were interviewed in a language other than English and 72.7% did not use English at home. Nearly half have been living in the U.S. for over 10 years (48.6%). Approximately 2% had a score of 13 or over indicating serious mental distress. Being interviewed in a language other than English, speaking English at home, having higher levels of school support and neighborhood cohesion, and not being threatened by peers were associated with current lower levels of mental distress in non U.S.-born adolescents. The findings of this study contribute to the literature and theory focusing on impacts of acculturation, school, and neighborhood on mental health of immigrant adolescents. Schools and communities may be the channels to help immigrant adolescents` acculturation process and ultimately to reduce their mental distress.

A NAT for reliable HCV RNA detection from plasma and plasma-derived medicinal products

Hong SeungHwa,Yoo SiHyung,Jung SaRah,Park SuJin,Kim SunNam,Hong ChoongMan,Lee KiHong,Oh HoJung,Kang HyeNa,Lee SeokHo,Hong SungRan,Choi SeungEun,Shin InSoo 大韓藥學會 2002 大韓藥學會 總會 및 學術大會 Vol.2002 No.2

전복 보호 해석 모델 개발에 관한 연구

홍석호(Seokho Hong),임장묵(Jangmook Lim),박형욱(Hyungwook Park) 한국자동차공학회 2008 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Rollover Protection CAE model of SUV was developed using MADYMO in this paper. The vehicle model includes a detailed description of the suspension system. The model was correlated by Drop Test. The correlated model was used to simulate untripped (Embankment, Corkscrew) and tripped (Curb-Trip, Soil-Trip) rollover, the results of simulation was compared with experimental data obtained from these test modes. Use of the MADYMO based models help to support rollover sensing algorithm development and set up test Matrix for rollover sensor testing.