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Scavenging Effect of Plant-Derived Materials on Free Radieals and Active Oxygen Species
Lee, Hoi Seon,Lee, Sung Eun,Ahn, Young Joon 한국농화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.1
The in vitro antioxidative activities of the 33 plant-derived essential oils and 37 phytochemicals including 3 Mentha arvensis leaf-, 2 Thymus vulgaris leaf- and 2 Syzygium aromaticum flower-derived isolates were determined by the inhibition of linoleic acid autoxidation, the generation of superoxide anion and scavenging of DPPH radical. They were then compared to those of the widely used plantderived antioxidants (pyrogallol and quercetin) and synthetic antioxidant BHT. At a concentration of 0.01%, potent antioxidative effect was observed in the essential oils from Cinnamomum cassia roots, Mentha arvensis leaves, Ginkgo biloba fruits, and Syzygium aromaticum flowers. Of the phytochemicals used, eugenol and isoeugenol at 0.01% showed potent antioxidative activity, and their activities were comparable to those of pyrogallol, quercetin, and BHT. The Cinnamomum root-, the Mentha leaf-, the Ginkgo fruit-, and the Syzygium flower-derived materials may be a good source for an alternative to the currently used antioxidants.
Cytotoxic Activity of Leguminous Seed Extracts against Human Tumor Cell Lines
( Hoi Seon Lee,Jeong Ock Lee,Hee Kwon Lee,Jong Hwan Oh,Young Joon Ahn ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.4
The cytotoxic activity of methanol extracts of 25 leguminous seeds in vitro was evaluated by sulforhodamine B assay, using the five human solid A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line arid leguminous seed used. Extracts of Canavalia lineata and Glycine soja revealed potent cytotoxic activity against A549 arid SK-MEL-2 cell lines. Moderate activity was observed in the extracts of Cassia obtusifolia and Glyeine max var. chungtae, and C. lineata and Vigna angulasis against SK-MEL-2 and HCT-15 cell lines, respectively. The other seed extracts were ineffective against model tumor cell lines. Because of their potent cytotoxic activities, the activity of each solvent fraction from C. lineata and G. soja was determined and the potent activity was produced from their chloroform fractions. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new types of anti-tumor agents.
Attractive Responses of High Power Light Emitting Diodes against Plutella xylostella Adult
Hoi-Seon Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10
To evaluate potential attractive activity of high power light emitting diodes (HPLEDs) against Plutella xylostella (Lepidoptera: Plutellidae), the specific wavelengths, illuminance intensities (20, 40, 60, 80, 100 lux), and light exposure times were investigated at 5 minute intervals. The evaluated specific wavelengths were blue (450±10 nm), green (520±5 nm), yellow (590±5 nm), red (660±10 nm), and white (450-630 nm). Based on the highest attraction rate, the HPLEDs treated to 60 lux intensity against P. xylostella were significantly more attractive than other illuminance intensities when light exposure time was 10 min. Attraction rate under optimal conditions showed that the green HPLED had the highest attraction rate (98.3%), followed by red HPLED (89.3%), blue HPLED (86.7%), yellow HPLED (76.7%), and white HPLED (70.0%), respectively. These results indicated that phototatic effect of the green HPLED against P. xylostella showed the greatest attraction at 60 lux intensity and 10 min light exposure time. Further research needs to be performed to evaluate the phototatic behavioral responses to single and multiple HPLED sources in the field.
Suppression Effect of Purpurin Derivatives on Nitric Oxide Synthase
Lee, Hoi-Seon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
The inhibitory effects of Rubia tinctorum root-derived materials against nitric oxide (NO) production were assessed by evaluation of NO production and inducible NO synthase (iNOS) expression, and compared to those of trihydroxyanthraquinone derivatives. The inhibitory effect of 1,2,4-trihydroxyanthraquinone (purpurin) from R. tinctorum roots against NO production was 60.7 and 38.5% at 2.5 and $1.25{\mu}g/mL$, respectively. Inhibitory effect of 1,2,7-trihydroxyanthraquinone was 52.5% at $2.5{\mu}g/mL$. Suppression effects of 1,2,4-trihydroxyanthraquinone on iNOS expression were confirmed by western blot analysis.