Protective effects of a novel herbal decoction on focal cerebral ischemia in a rodent model

Kim, Myung-Gyou,Choi, Jae-Hwan,Lim, Jong-Pil,Kim, Dae-Keun,Shin, Tae-Yong,Boo, Yungmin,Kim, Sun-Yeou,Kim, Hocheol,Ha, Eunyoung,Park, Hun-Kuk,Kim, Jeongseon,Lim, Ha-Sup,Kim, Ee-Hwa,Kim, Jeung-Beum,Leem Informa UK (TaylorFrancis) 2007 Neurological research Vol.29 No.suppl1

PAL-XFEL 빔라인 허치 구조물 개발

김승남(Seungnam Kim),김명진(Myeongjin Kim),김성한(Seonghan Kim),김영찬(Yeongchan Kim),신호철(Hocheol Shin),김지화(Jihwa Kim),김경숙(Kyeongsuk Kim),김광우(Kwangwoo Kim),엄인태(Intae Eom) 한국소음진동공학회 2016 한국소음진동공학회 논문집 Vol.26 No.5

The hutches which are installed in the beamline are largely classified into two, i.e XPP (X-ray pump probe) and CXI (Coherent X-ray image). Laser room is installed on the hutch and provides laser to XPP and CXI simultaneously. And two hutches have heavy crane to install some optics equipments. Safety and reliability of hutch structures should be taken into account for the precise operating of the laser facilities, so vibration analysis is essential to do this. The main purpose of vibration analysis is to install hutch structures with large stiffness. We have changed materials specification several times to install hutch structures having strong stiffness. Now hutch structures were installed and checked vibration status at laser room and XPP hutch. The results of laser table and robot arm satisfy vibration criteria. This paper explains about the design and vibration analysis of hutch structures.

Lung Transplantation in Six Patients with Idiopathic Pulmonary Artery Hypertension

( Hyeonhwa Kim ),( Dongkwan Kim ),( Sehoon Choi ),( Geundong Lee ),( Dongkyu Oh ),( Hocheol Kim ),( Jaeseung Lee ),( Sungho Jung ),( Pilje Kang ),( Won Kim ),( Seungil Park ),( Sangbum Hong ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0

Idiopathic pulmonary artery hypertension (IPAH) is an incurable and invariably fatal disease. Lung transplantation is a useful therapeutic option in patients who are unresponsive to medical treatment; however, lung transplantation performed for pulmonary hypertension is associated with significantly high perioperative mortality rates. We report a case series of six patients who underwent lung transplantation for IPAH between October 2008 and June 2021. Patients’ median age was 28.5 years, and the study included 5 of 6 women (83%). Pre-transplantation hemodynamic parameters showed mean right atrial pressure of 12.0±7.1 mmHg and mean pulmonary artery pressure of 62.2±29.5 mmHg. Two of six patients received extracorporeal membrane oxygenation (ECMO) therapy as a bridge to transplantation over 14 and 17 days, and four patients underwent elective transplantation. Two patients required prolonged postoperative venoarterial (V-A) ECMO support. Grade 3 primary graft dysfunction occurred in one patient; however, the clinical course improved following prolonged V-A ECMO therapy. Five patients (83.3%) required intervention for postoperative bleeding control; one of these patients died of uncontrolled bleeding concomitant with disseminated intravascular coagulation, on the 14th postoperative day, and we observed no other perioperative deaths. One patient died of carbapenem-resistant Acinetobacter baumannii bacteremia, a year postoperatively. The 1-month, 6-month, and 1-year survival rates were 83.3%, 83.3%, and 66.7%, respectively. In view of the poor prognosis of IPAH, lung transplantation (1-year mortality rates <40%) merits consideration as a useful therapeutic option in this patient population. However, postoperative bleeding tends to occur in most patients; therefore, close monitoring is important during post-transplantation management.

Neuroprotective effect of HT008-1, a prescription of traditional Korean medicine, on transient focal cerebral ischemia model in rats

Bu, Youngmin,Kwon, Sungeun,Kim, Yun Tai,Kim, Mi-Yeon,Choi, Hoyoung,Kim, Jae Goo,Jamarkattel-Pandit, Nirmala,Doré,, Sylvain,Kim, Sung-Hoon,Kim, Hocheol John Wiley Sons, Ltd. 2010 Phytotherapy research Vol.24 No.8

<P>HT008-1 is one of the prescriptions used in Traditional Korean Medicine for the treatment of mental and physical weakness. It is composed of Panax ginseng, Acanthopanax senticosus, Angelica sinensis and Scutellaria baicalensis, which have been reported to have various pharmacological effects on the central nervous system. The study investigated whether HT008-1 has neuroprotective effects in a focal cerebral ischemia rat model. Stroke was induced in rats by 2 h of middle cerebral artery occlusion (MCAo) followed by 22 h of reperfusion. HT008-1 (30, 100 and 300 mg/kg) and the component herbs (300 mg/kg) were administered orally twice at 0 and 2 h after ischemia. Oral administration of 300 mg/kg HT008-1 reduced brain infarction by 45.7%, prolonged the latency time by 24.8% in the rotarod test, and enhanced the score by 17.0% in the balance beam test. Only P. ginseng and S. baicalensis showed protective effects, and HT008-1 showed a greater effect than its component herbs. HT008-1 down-regulated the COX-2 and OX-42 expression in the penumbra region. In conclusion, the results show that HT008-1 may be effective in a rat stroke model by an antiinflammatory mechanism and may improve sensory-motor function by reducing damage in the cortex and caudoputamen. Copyright © 2010 John Wiley & Sons, Ltd.</P>

Epitaxial van der Waals Contacts between Transition-Metal Dichalcogenide Monolayer Polymorphs

Lee, Chang-Soo,Oh, Seung Jae,Heo, Hoseok,Seo, Seung-Young,Kim, Juho,Kim, Yong Hyeon,Kim, Donghwi,Ngome Okello, Odongo Francis,Shin, Hocheol,Sung, Ji Ho,Choi, Si-Young,Kim, Jun Sung,Kim, Jong Kyu,Jo, M American Chemical Society 2019 Nano letters Vol.19 No.3

<P>We have achieved heteroepitaxial stacking of a van der Waals (<I>vdW</I>) monolayer metal, 1T’-WTe<SUB>2</SUB>, and a semiconductor, 2H-WSe<SUB>2</SUB>, in which a distinctively low contact barrier was established across a clean epitaxial <I>vdW</I> gap. Our epitaxial 1T’-WTe<SUB>2</SUB> films were identified as a semimetal by low temperature transport and showed the robust breakdown current density of 5.0 × 10<SUP>7</SUP> A/cm<SUP>2</SUP>. In comparison with a series of planar metal contacts, our epitaxial <I>vdW</I> contact was identified to possess intrinsic Schottky barrier heights below 100 meV for both electron and hole injections, contributing to superior ambipolar field-effect transistor (FET) characteristics, i.e., higher FET mobilities and higher on-off current ratios at smaller threshold gate voltages. We discuss our observations around the critical roles of the epitaxial <I>vdW</I> heterointerfaces, such as incommensurate stacking sequences and absence of extrinsic interfacial defects that are inaccessible by other contact methods.</P> [FIG OMISSION]</BR>

Human lung microbial communities are associated with clinical outcome in patients with idiopathic pulmonary fibrosis

( Hocheol Kim ),( Hee-young Yoon ),( Kwanghun Choi ),( Jin-woo Bae ),( Jin Woo Song ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.0

Purpose: Human lung microbiome are reported to be associated with survival, and acute exacerbation in patients with idiopathic pulmonary fibrosis (IPF). However, composition and clinical impact of microbiobial communities in the IPF lung tissues are unknown. The study aimed to investigate the association between lung tissue microbiome and clinical outcome in IPF patients. Methods: This study included patients with IPF (n=20; 10 survivors and 10 non-survivors) and age- and sex-matched controls (n=20). The genomic DNA was extracted from human lung tissues and amplified using fusion primers targeting variable V3 and V4 regions of the 16S RNA genes with indexing barcodes. Results: The median follow-up period was 5.4 years. The mean age of IPF subjects was 63.3 years, and 65% were male. There were no significant differences in alpha diversity indices between IPF patients and controls, and between survivors and non-survivors among IPF patients. In IPF lung, the relative abundance of genus Lactobacillus, Paracoccus, and Akkermansia increased, whereas that of genus Caulobacter, Azonexus, Undibacterium decreased compared with that in controls. In IPF non-survivors, genus Streptococcus and Bifidobacterium significantly increased than those in IPF survivors. In IPF patients, forced vital capacity (FVC) showed positive correlation with relative abundance of genus Curvibacter (r = 0.590) and Thioprofundum (r = 0.458), and negative correlation with that of genus Anoxybacillus (r= - 0.509), Enterococcus (r = - 0.593), Akkermansia (r = - 0.505), and Clostridium (r = - 0.445). In the multivariate Cox analysis adjusted by age, sex, smoking, and FVC, the relative abundance of genus Streptococcus (hazard ratio [HR]: 1.993, p = 0.044), Sphingomonas (HR: 57.590, p = 0.024), and Clostridium (HR: 37.189, p = 0.038) were significantly associated with IPF mortality. Conclusions: Our results suggest that lung tissue microbial communities could affect disease severity and prognosis in patients with IPF.

Meta-analysis of genome-wide SNP- and pathway-based associations for facets of neuroticism

Kim, Song E,Kim, Han-Na,Yun, Yeo-Jun,Heo, Seong Gu,Cho, Juhee,Kwon, Min-Jung,Chang, Yoosoo,Ryu, Seungho,Shin, Hocheol,Shin, Chol,Cho, Nam H,Sung, Yeon Ah,Kim, Hyung-Lae Nature Publishing Group 2017 Journal of human genetics Vol.62 No.10

<P>Neuroticism is a heritable personality trait that is comprised of distinct sub-factors, or facets. Sub-factors of neuroticism are linked to different emotional states or psychiatric symptoms and studying the genetic variants associated with these facets may help reveal the biological mechanisms underlying psychiatric disorders. In the present study, a meta-analysis of genome-wide association studies for six facets of neuroticism was performed in 5584 participants from three cohorts. Additionally, a Gene Set Enrichment Analysis was conducted to find biological pathways associated with each facet. Six neuroticism facets (N1: anxiety, N2: angry hostility, N3: depression, N4: self-consciousness, N5: impulsivity and N6: vulnerability) were assessed using the Korean version of the Revised NEO Personality Inventory. In the single-nucleotide polymorphism-based analysis, results showed genome-wide significance for N2 within the <I>MIR548H3</I> gene (rs1360001, <I>P</I>=4.14 × 10<SUP>−9</SUP>). Notable genes with suggestive associations (<I>P</I><1.0 × 10<SUP>−6</SUP>) were <I>ITPR1</I> for N1, <I>WNT7A</I> for N2, <I>FGF10</I> and <I>FHIT</I> for N3, <I>DDR1</I> for N4, <I>VGLL4</I> for N5 and <I>PTPRD</I> for N6. In the pathway-based analysis, the axon guidance pathway was identified to be associated with multiple facets of neuroticism (N2, N4 and N6). The focal adhesion and extracellular matrix receptor interaction pathways were significantly associated with N2 and N3. Our findings revealed genetic influences and biological pathways that are associated with facets of neuroticism.</P>

Mori Folium and Mori Fructus Mixture Attenuates High-Fat Diet-Induced Cognitive Deficits in Mice

Kim, Hyo Geun,Jeong, Hyun Uk,Park, Gunhyuk,Kim, Hocheol,Lim, Yunsook,Oh, Myung Sook Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P>Obesity has become a global health problem, contributing to various diseases including diabetes, hypertension, cancer, and dementia. Increasing evidence suggests that obesity can also cause neuronal damage, long-term memory loss, and cognitive impairment. The leaves and the fruits of <I>Morus alba</I> L., containing active phytochemicals, have been shown to possess antiobesity and hypolipidemic properties. Thus, in the present study, we assessed their effects on cognitive functioning in mice fed a high-fat diet by performing immunohistochemistry, using antibodies against c-Fos, synaptophysin, and postsynaptic density protein 95 and a behavioral test. C57BL/6 mice fed a high-fat diet for 21 weeks exhibited increased body weight, but mice coadministered an optimized Mori Folium and Mori Fructus extract mixture (2 : 1; MFE) for the final 12 weeks exhibited significant body weight loss. Additionally, obese mice exhibited not only reduced neural activity, but also decreased presynaptic and postsynaptic activities, while MFE-treated mice exhibited recovery of these activities. Finally, cognitive deficits induced by the high-fat diet were recovered by cotreatment with MFE in the novel object recognition test. Our findings suggest that the antiobesity effects of MFE resulted in recovery of the cognitive deficits induced by the high-fat diet by regulation of neural and synaptic activities.</P>

Key compound groups for the neuroprotective effect of roots of Polygonum cuspidatum on transient middle cerebral artery occlusion in Sprague-Dawley rats.

Kim, Jinmo,Kim, Mi-Yeon,Leem, Kang-Hyun,Moon, Sangkwan,Jamakattel-Pandit, Nirmala,Choi, Hoyoung,Kim, Hocheol,Bu, Youngmin Taylor Francis Health Sciences 2010 NATURAL PRODUCT RESEARCH Vol.24 No.13

<P>Polygonum cuspidatum is a potent anti-oxidant herb that is well known for its various bioactivities. The current study investigates which compound group is most effective, to establish the key compound groups for quality assessment, especially in terms of neuroprotective effects. The roots of P. cuspidatum were extracted with 85% methanol and fractionated with hexane, ethyl acetate, n-butanol and water. Each fraction was applied to an in vitro radical scavenging assay, a lipid peroxidation assay in brain homogenates and an in vivo assay using a transient focal cerebra ischemia model induced by a middle cerebral artery occlusion in a Sprague-Dawley rat. The ethyl acetate fraction was the most effective fraction in both in vitro and in vivo assays, having the highest stilbene and anthraquinone contents. These results suggest that stilbenes and anthraquinones may be key compound groups for the quality assessment of the anti-oxidative and neuroprotective effects of P. cuspidatum.</P>

Correlation between gut microbiota and personality in adults: A cross-sectional study

Kim, Han-Na,Yun, Yeojun,Ryu, Seungho,Chang, Yoosoo,Kwon, Min-Jung,Cho, Juhee,Shin, Hocheol,Kim, Hyung-Lae Elsevier 2018 Brain, behavior, and immunity Vol.69 No.-

<P><B>Abstract</B></P> <P>Personality affects fundamental behavior patterns and has been related with health outcomes and mental disorders. Recent evidence has emerged supporting a relationship between the microbiota and behavior, referred to as brain-gut relationships. Here, we first report correlations between personality traits and gut microbiota. This research was performed using the Revised NEO Personality Inventory and the sequencing data of the 16S rRNA gene in 672 adults. The diversity and the composition of the human gut microbiota exhibited significant difference when stratified by personality traits. We found that personality traits were significantly correlated with diversity of gut microbiota, while their differences were extremely subtle. High neuroticism and low conscientiousness groups were correlated with high abundance of Gammaproteobacteria and Proteobacteria, respectively when covariates, including age, sex, BMI and nutrient intake, were controlled. Additionally, high conscientiousness group also showed increased abundance of some universal butyrate-producing bacteria including Lachnospiraceae. This study was of observational and cross-sectional design and our findings must be further validated through metagenomic or metatranscriptomic methodologies, or metabolomics-based analyses. Our findings will contribute to elucidating potential links between the gut microbiota and personality, and provide useful insights toward developing and testing personality- and microbiota-based interventions for promoting health.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Personality traits were correlated with gut microbiota composition. </LI> <LI> Gammaproteobacteria was increased in high neuroticism group. </LI> <LI> Low conscientiousness group showed increased abundance of Proteobacteria. </LI> <LI> The low conscientiousness group showed decreased abundance of Lachnospiraceae. </LI> </UL> </P>