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허성남,민경희,유재근,최순영 숙명여자대학교 자연과학연구소 1999 자연과학논문집 Vol.- No.10
팔당호의 생태학적 환경요인의 계절적인 변화를 분석하므로서 이것으로 인한 식물성 플랑크톤의 연관성을 고찰과 함께 오염과정을 파악하여 수질오염의 예방의 기초자료를 얻고자 본 실험을 수행하였다. 1994년 4월부터 1995년 10월까지 계절별로 3개 지점에서 시료를 채취하여 표층부와 저층부의 수질을 분석하였다 용존산소(DO)와 pH는 각각 7.0-11.6 ㎎/ℓ와 6.9-8.9의 범위이었으며 COD와 BOD농도는 각각 1.6-4.8 ㎎/ℓ와 0.8-2.9 ㎎/ℓ이었으며,SS는 1.8-8.6 ㎎/ℓ의 농도차를 보여 주었다. 또한 NH₃-N와 PO₄-P의 농도는 각각 0.161-0.478 ㎎/ℓ와 0.003-0.073 ㎎/ℓ이었다. 표층의 chlorophyll a의 농도는 낮게는 북한강 유입부위인 12.3 ㎎/ℓ로부터 가장 높은 경안천 유입부위 23.8 ㎎/ℓ의 범위를 보여주고 있다. pH,전도도, COD, BOD, SS ,총인, chlorophyll a의 농도는 하계절에 가장 높았으며 이 결과로 미루어 보아 하계절에는 하천에 오염물질이 가장 많이 유입되는 것으로 추정된다. Station별로 조사한 결과 이들의 농도는 경안천 하류인 St. 3가 남한강 하류인 St. 2이나 댐지역의 St. 4보다 높은 결과를 보여 주었으므로 경안천 상류에서 오염원이 가장 많이 유입되는 것으로 추정된다. 아울러 종속영양세균의 분포도 경안천이 다른 지역보다 가장 높게 나타났다. Seasonal variation of water quality was examined in the reservoir, lake Paldang on Han River, the inlet stations from three rivers, North Han River, South Han liver, Kyung-An stream, and the station of dam area of the lake. Water samples were collected bimonthly from surface and bottom layers at four different stations of the lake Paldang from April 1993 to August 1994. With respect to the seasonal variation, pH, conductivity, and concentrations of COD, BOD, SS, total phosphorus, and chlorophyll a were higher in summer compared with those of autumn or other seasons, while concentrations of DO, NH₃-N, and PO₄-P were increased in winter. Concentrations of COD, BOD, chlorophyll a, total nitrogen and phosphorus were the highest at the station of Kyung-An stream rather than those of other three stations. The average concentrations of chlorophyll a surface water were from 12.3 ㎎/ℓ at North Han River to 23.8 ㎎/ℓ at Kyung-An stream. Heterotrophic bacterial distribution showed higher at the station of Kyung-An steam than those of South Han River and the dam station of the lake.
Diesel 機關에 있어서 熱發生率을 利用한 最適 噴射時期 및 壓力에 關한 實驗的 硏究
盧相舜,吳永澤,許柄武 全北大學校 1987 論文集 Vol.29 No.-
The purpose of this study is to examine the effects of many factors by means of rate of heat release calculation in Diesel Engine. In this study, first, rate of heat release was calculated with computer process, next, the effects of those factors to rate of heat release were examined, and then, the effects of injection pressure and injection time to rate of heat release were analyzed. The concluding remarks are as the followings. 1. To calculate the precise rate of heat rlease, compensation of air column vibration and the method of least square were used. 2. On calculating the rate of beat release, specific heat is variable with function of temperature. 3. Controlling of engine performance and smoke was shown to a fixed quantity by means of analyzing indicator diagram. 4. BTDC 9˚CA and 12.7 MPa are most suitable injection time and injection pressure to get a max. engine performance. 5. When the injection time is earlier than that time, knocking was occured, because of high combustion max.-pressure and high rate of pressure rise. When the injetion time is later than TDC, the engine performance due to decreasing the degree of constant volume is decreased. 6. In case too low injection pressure, engine performance is decreased, and the injection pressure is too high engine performance is also decreased.
방사선 조사가 배양된 조골세포의 apoptosis와 세포주기의 변화 및 석회화 결절 형성에 미치는 영향에 관한 연구
이영미,최항문,허민석,이삼선,최순철,박태원 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.3
Purpose : The study was aimed to detect the induction of apoptosis, cell cycle arrest and calcified nodule formation after irradiation on primarily cultured osteoblasts. Materials and Methods : Using rat calvarial osteoblasts, the effects of irradiation on apoptosis, cell cycle arrest, and calcified nodule formation were studied. The single irradiation of 10 and 20 Gy was done with 5.38 Gy/min dose rate using the 137Cs cell irradiator at 4th and 14th day of culture. Apoptosis induction and cell cycle arrest were assayed by the flowcytomtry at 1,2,3, and 4 days after irradiation. The formation of calcified nodules was observed by alizarin red staining at 1,3,10,14 days after irradiation at 4th day of culture, and at 1,4,5 days after irradiation at 14th day of culture. Results : Apoptosis was not induced by 10 or 20 Gy independent of irradiation and culture period. Irradiation did not induced Gl arrest in post-irradiated ostedblasts. After irradiation at 4th-day of culture, G2 arrest was induced but it was not statistically significant after irradiation at 14th-day of culture. In the case of irradiated cells at 4th day of culture, calcified nodules were not formed and at 14th-day of culture after irradiation, calcified nodule formation did not affected. Conclusion : Taken together, these results suggest that Irradiation at the dose of 10-20 Gy would not affect apoptosis induction of osteoblasts. Cell cycle and calcified nodule formation were influenced by the level of differentiation of osteblasts. (Korean J Oral Maxillofac Radiol 2000; 30: 189-198)
모터 제어ㆍ감시를 위한 Monitoring Device 개발
정도영,박영록,심낙순,박종국,허규환 삼척대학교 산업과학기술연구소 2001 産業科學技術硏究論文集 Vol.6 No.1
A motor used as power source by factory has a relay panels up to recently. But they have the problems of operating and maintenance, such as mechanical contracts, etc. They also need some additional relays to check the over load, over currents and ground faults. But in the development of computers the relay panels to control motor are changed by electrical motor controllers with microprocessor or DSP. They can check the event, the over load, over currents and ground faults and also supervise the trend and state of operating. In this paper, a monitoring devices for the control and supervision of motor has been presented and developed.
Heo, Soon-Young,Ahn, Kwang-Sung,Kang, Jee-Hyun,Shim, Ho-Sup The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.2
Recent studies on nuclear transfer and induced pluripotent stem cells have demonstrated that differentiated somatic cells can be returned to the undifferentiated state by reversing their developmental process. These epigenetically reprogrammed somatic cells may again be differentiated into various cell types, and used for cell replacement therapies through autologous transplantation to treat many degenerative diseases. To date, however, reprogramming of somatic cells into undifferentiated cells has been extremely inefficient. Hence, reliable markers to identify the event of reprogramming would assist effective selection of reprogrammed cells. In this study, a transgene construct encoding enhanced green fluorescent protein (EGFP) under the regulation of human Oct4 promoter was developed as a reporter for the reprogramming of somatic cells. Microinjection of the transgene construct into pronuclei of fertilized mouse eggs resulted in the emission of green fluorescence, suggesting that the undifferentiated cytoplasmic environment provided by fertilized eggs induces the expression of EGFP. Next, the transgene construct was introduced into human embryonic fibroblasts, and the nuclei from these cells were transferred into enucleated porcine oocytes. Along with their in vitro development, nuclear transfer embryos emitted green fluorescence, suggesting the reprogramming of donor nuclei in nuclear transfer embryos. The results of the present study demonstrate that expression of the transgene under the regulation of human Oct4 promoter coincides with epigenetic reprogramming, and may be used as a convenient marker that non-invasively reflects reprogramming of somatic cells.
Soon Young Heo,Kwang Sung Ahn,Jee Hyun Kang,Hosup Shim 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.2
Recent studies on nuclear transfer and induced pluripotent stem cells have demonstrated that differentiated somatic cells can be returned to the undifferentiated state by reversing their developmental process. These epigenetically reprogrammed somatic cells may again be differentiated into various cell types, and used for cell replacement therapies through autologous transplantation to treat many degenerative diseases. To date, however, reprogramming of somatic cells into undifferentiated cells has been extremely inefficient. Hence, reliable markers to identify the event of reprogramming would assist effective selection of reprogrammed cells. In this study, a transgene construct encoding enhanced green fluorescent protein (EGFP) under the regulation of human Oct4 promoter was developed as a reporter for the reprogramming of somatic cells. Microinjection of the transgene construct into pronuclei of fertilized mouse eggs resulted in the emission of green fluorescence, suggesting that the undifferentiated cytoplasmic environment provided by fertilized eggs induces the expression of EGFP. Next, the transgene construct was introduced into human embryonic fibroblasts, and the nuclei from these cells were transferred into enucleated porcine oocytes. Along with their in vitro development, nuclear transfer embryos emitted green fluorescence, suggesting the reprogramming of donor nuclei in nuclear transfer embryos. The results of the present study demonstrate that expression of the transgene under the regulation of human Oct4 promoter coincides with epigenetic reprogramming, and may be used as a convenient marker that non-invasively reflects reprogramming of somatic cells.